Amino acid alphabet size in protein evolution experiments: better to search a small library thoroughly or a large library sparsely?

We compare the results obtained from searching a smaller librarythoroughly versus searching a more diverse, larger library sparsely.We study protein evolution with reduced amino acid alphabets,by simulating directed evolution experiments at three differentalphabet sizes: 20, 5 and 2. We employ a physical model forevolution, the generalized NK model, that has proved successfulin modeling protein evolution, antibody evolution and T-cellselection. We find that antibodies with higher affinity arefound by searching a library with a larger alphabet sparselythan by searching a smaller library thoroughly, even with well-designedreduced libraries. We also find ranked amino acid usage frequenciesin agreement with observations of the CDR-H3 variable regionof human antibodies.     

Life-cycle greenhouse gas emissions and energy balances of sugarcane ethanol production in Mexico

The purpose of this work was to estimate GHG emissions and energy balances for the future expansion of sugarcane ethanol fuel production in Mexico with one current and four possible future modalities. We used the life cycle methodology that is recommended by the European Renewable Energy Directive (RED), which distinguished the following five system phases: direct Land Use Change (LUC); crop production; biomass transport to industry; industrial processing; and ethanol transport to admixture plants. Key variables affecting total GHG emissions and fossil energy used in ethanol production were LUC emissions, crop fertilization rates, the proportion of sugarcane areas that are burned to facilitate harvest, fossil fuels used in the industrial phase, and the method for allocation of emissions to co-products. The lower emissions and higher energy ratios that were observed in the present Brazilian case were mainly due to the lesser amount of fertilizers applied, also were due to the shorter distance of sugarcane transport, and to the smaller proportion of sugarcane areas that were burned to facilitate manual harvest. The resulting modality with the lowest emissions of equivalent carbon dioxide (CO_2e) was ethanol produced from direct juice and generating surplus electricity with 36.8 kgCO_2e/GJ_ethanol. This was achieved using bagasse as the only fuel source to satisfy industrial phase needs for electricity and steam. Mexican emissions were higher than those calculated for Brazil (27.5 kgCO_2e/GJ_ethanol) among all modalities. The Mexican modality with the highest ratio of renewable/fossil energy was also ethanol from sugarcane juice generating surplus electricity with 4.8 GJ_ethanol/GJ_fossil.     

The role of technology transfer for the development of a local wind component industry in Chile

This paper contributes to the debate about climate change technology transfer by analysing barriers and enablers for a Chilean company starting up the production of wind blades. Literature on the role of technology transfer for the development and deployment of local renewable energy technologies in developing countries often refers to success stories in Brazil, India and China. Instead, this case study highlights the different challenges faced by smaller emerging economies. The paper argues that successful technology transfer in a smaller economy like Chile requires: a minimum internal demand and access to regional markets to attract foreign knowledge providers; a focus in the types of technologies where the recipient country or company have a competitive advantage; and active learning processes by the recipient company. Lessons are drawn for improving the design and implementation of technology-push and market-pull policies in small or medium emerging economies.     

NUMERICAL SIMULATION OF THE EFFECTS OF BUOYANCY FORCES AND FLOW CONTROL DEVICES ON FLUID FLOW AND HEAT TRANSFER PHENOMENA OF LIQUID STEEL IN A TUNDISH

Fluid flow and heat transfer of liquid steel in a tundish of a continuous slab caster was numerically simulated. The importance of natural flow convection was established through a dimensionless number given by the ratio Gr/Re². Buoyancy forces proved to be as important as inertial ones, especially in the extremes of the tundish far from the entering liquid jet. The usual flow control devices like weirs and dams were not as effective as turbulence inhibitors, a kind of impact pad with lips, interior squared angles, and a square cross section. The simulations indicated that this device helps to decrease fluid turbulence in the zone of the entering liquid jet and has damping effects of step inputs of cold or hot steel, allowing for better control of the casting temperature.     

Hydrogeographical Regionalisation: An Approach for Evaluating the Effects of Land Cover Change in Watersheds. A Case Study in the Cuitzeo Lake Watershed, Central Mexico

Efficient natural resources management, including continental water at watershed level, requires understanding the arrangement of landscape attributes in a region. The geographical analysis of landscape attributes is a useful approach to delineate relatively homogeneous watersheds or regions. This research was carried out in order to evaluate the effect of land cover through time on regionalisation modelling in the poorly-gauged Cuitzeo Lake Watershed, and to develop models to create two hydrogeographical regionalisations for the years 1975 and 2000. The inputs required by the regionalisation methodology were integrated in a GIS and validated before carrying out statistical regionalisation procedures (cluster analysis and PCA). GIS operations were done in Arc View 3.2 and statistical analyses in PC-ORD. Median Euclidean distances with mean distance linkage methods were used. A 75% of similarity was chosen as the threshold to generate regions. Importance rankings of regions were obtained using multicriteria evaluation methods. Based on the analysis, 38 of the 52 subwatersheds belonging to the Cuitzeo Lake Watershed were clustered; eight groups were defined in 1975 and nine in 2000. According to PCA, the strongest positive variables are associated to morphometric, geologic, land cover and soil attributes. Fourteen subwatersheds were never clustered. Four subwatersheds changed of cluster between 1975 and 2000. This change is explained because of rainfed agricultural parcel abandonment, and subsequent shrubland growth. This means that the hydrogeographical regionalisation is sensitive to land cover change processes. The methodological approach applied in this research is a low-cost and fast alternative for evaluating the impact of land cover and land use change on hydrogeographical regionalisation; in consequence, data and information generated during the analysis were made available to local authorities so that they can improve both water resources planning and their informational baseline for decision making and for development of environmental policies in the Cuitzeo Lake Watershed.     

A practical approach to electrode-skin impedance unbalance measurement

Unbalance between electrode-skin impedances is a major problem in biopotential recordings, leading to increased power-line interference. This paper proposes a simple, direct method to measure that unbalance at power-line frequency (50-60 Hz), thus allowing the determination of actual recording conditions for biopotential amplifiers. The method is useful in research, amplifier testing, electrode design and teaching purposes. It has been experimentally validated by using both phantom impedances and real electrode-skin impedances.     

A proactive backup scheme for reliable real-time transmission

Reliable transmission is a key issue for distributed real-time applications. The concept of Real-time Dependable Channel was introduced to provide availability to real-time transmission. Two aspects are important for the efficiency of a Real-time dependable channel: assuring the end-to-end delay bound and optimising the utilisation of network resources. A packet can miss its delay bound for two reasons: network congestion or network failure. The classic solution to this problem has been the use of Backup Channels which introduces the notion of availability at the expense of increasing the use of network resources. However, this over-provisioning of resources is potentially wasted, since the failure rate is very low.     

One year of ICTP diploma courses on-line using the automated EyA recording system

The 12-month pre-Ph.D. ICTP Diploma Courses in the fields of Condensed Matter Physics, High Energy Physics, Mathematics, Earth System Physics and Basics Physics have been recorded using the automated, low cost recording system called EyA developed in-house. We discuss the technical details on how these recordings were implemented, together with some web usage statistics and students feedback. As yet, no similar endeavor has been made to put on-line a complete high-level Diploma Programme, due to the high costs involved when using alternative recording solutions. These recordings are freely available on the website www.ictp.tv.