Impact of an exercise program on acylcarnitines in obesity: a prospective controlled study

Background

Acylcarnitine (AC) transport dysfunction into the mitochondrial matrix is one of the pathophysiological mechanisms of type 2 diabetes mellitus (DM). The effect of an aerobic exercise (AE) program on this condition in obese subjects without DM is unclear.

Methods

A prospective, randomized, longitudinal, interventional study in a University Research Center involved a 10-week AE program in 32 women without DM and a body mass index (BMI) greater than 27?kg/m². (Cases n?=?17; Controls n?=?15). The primary objective was to evaluate the influence of a controlled AE program on beta-oxidation according to modifications in short, medium, and long-chain ACs. Secondary objectives were to define the behavior of amino acids, and the correlation between these modifications with metabolic and anthropometric markers.

Results

The proportion of dropouts was 17% and 6% in controls and cases, respectively. In cases there was a significant reduction in total carnitine (30.40 [95% CI 28.2 to 35.6]) vs. (29.4 [CI 95% 25.1 to 31.7]) p?=?0.0008 and long-chain AC?C14 (0.06 [95% CI 0.05 to 0.08]) vs. (0.05 [95% CI 0.05 to 0.09]) p?=?0.005 and in C18 (0.31 [95% CI 0.27 to 0.45]) vs. (0.28 [95% CI 0.22 to 0.32]) p?=?0.03. Free fatty acid levels remained without change during the study in both groups.

Conclusion

In conclusion, a controlled 10-week AE program improved beta-oxidation by reducing long-chain ACs. This finding highlights the importance that AE might have in avoiding or reverting lipotoxicity, and in consequence, improving insulin sensitivity and pancreatic beta cell functional reserve.     

Synthesis and Investigation of Tryptophan–Amphotericin B Conjugates

Anchors aweigh! The synthesis of tryptophan–amphotericin B conjugates (see figure) is described. The membrane‐anchoring effect of tryptophane was thus combined with the pore‐formation effect of amphotericin B leading to high channel activity in sterol‐free liposomes.

     

Improved cellulose formation by an Acetobacter xylinum mutant limited in (keto)gluconate synthesis

A mutation and enrichment strategy is described that allows the iso-lation of Acetobacter xylinum mutant strains which are restricted in (keto)gluconate synthesis and produce cellulose more efficiently, even under oxidative conditions. Acetobacter xylinum mutant strains obtained in this way may be very useful in the study of cellulose (over)synthesis and (keto)gluconate metabolism in Acetobacter xylinum, as well as in the improvement of bacterial cellulose fermentation processes.     

Recovery of semivolatile organic compounds during sample preparation: implications for characterization of airborne particulate matter

Semivolatile compounds present special analytical challenges not met by conventional methods for analysis of ambient particulate matter (PM). Accurate quantification of PM-associated organic compounds requires validation of the laboratory procedures for recovery over a wide volatility and polarity range. To meet these challenges, solutions of n-alkanes (nC12-nC40) and polycyclic aromatic hydrocarbons PAHs (naphthalene to benzo[ghi]perylene) were reduced in volume from a solvent mixture (equal volumes of hexane, dichloromethane and methanol), to examine recovery after reduction in volume. When the extract solution volume reached 0.5 mL the solvent was entirely methanol, and the recovery averaged 60% for n-alkanes nC12-nC25 and PAHs from naphthalene to chrysene. Recovery of higher MW compounds decreased with MW, because of their insolubility in methanol. When the walls of the flasks were washed with 1 mL of equal parts hexane and dichloromethane (to reconstruct the original solvent composition), the recovery of nC18 and higher MW compounds increased dramatically, up to 100% for nC22-nC32 and then slowly decreasing with MW due to insolubility. To examine recovery during extraction of the components of the High Capacity Integrated Gas and Particle Sampler, the same standards were used to spike its denuders and filters. For XAD-4 coated denuders and filters, normalized recovery was >95% after two extractions. Recovery from spiked quartz filters matched the recovery from the coated surfaces for alkanes nC18 and larger and for fluoranthene and larger PAHs. Lower MW compounds evaporated from the quartz filter with the spiking solvent. This careful approach allowed quantification of organics by correcting for volatility- and solubility-related sample preparation losses. This method is illustrated for an ambient sample collected with this sampler during the Texas Air Quality Study 2000.     

Brush-like fibers on the human chromosome periphery

A brush-like border apparently composed of fibers protruding from metaphase chromosomes of human lymphocytes was observed for the first time using transmission (TEM) and scanning electron microscopy (SEM). On the basis of size and sensitivity to colcemid, the fibers may be related to microtubules and spindle organization. The brush-like fibers were observed when chemically fixed metaphase chromosome spreads were placed on glass slides and maintained under "wet" conditions (not allowed to air dry after fixation for conventional cytogenetic protocols) until postfixation protocols for TEM and SEM were performed. The purpose of this study was to establish the occurrence of the brush-like fibers and to determine the effects of colcemid on these fibers.     

Fermentation patterns of gramicidin S formation by Bacillus brevis ATCC 9999 in high-yielding synthetic medium

Addition of the gramicidin S (GS)-constituent amino acids, other than the limiting precursor L -phenylalanine, to the high-yielding chemically defined F3/6 and G3/6 media, enhanced growth and volumetric GS-production by Bacillus brevis ATCC 9999 considerably, but did not yield a higher specific GS-production level. L -Leucine alone could duplicate this stimulatory effect in G3/6 medium. Replacing the fructose component of F3/6 medium by these four amino acids yielded a high specific GS-production level, but resulted in poor growth and low volumetric antibiotic production. Nutrient-utilisation patterns in F3/6 medium revealed that B. brevis initially grew at the expense of L -glutamine and L -arginine. After a diauxic lag period, D -fructose was consumed together with L -histidine. L -Proline was mainly used during the stationary phase. L -Methionine was broken down gradually throughout the whole fermentation cycle. L -Phenylalanine was metabolised only after GS formation started, and its disappearance was proportional to the amount of GS produced. Lowering the aeration rate caused an acidification of the medium, resulting in a slower and incomplete, although similar, nutrient-utilisation pattern. At a controlled pH of 7.3, under lowered aeration, utilisation patterns were again comparable with those of a fully aerated fermentation, but GS levels were enhanced considerably (0.220 mg of GS mg^?1 dry cell wt). Depending on environmental culture conditions, B. brevis also excreted different amino acids (L -lysine, L -alanine, L -valine, L -serine), which were in turn metabolised during late growth and differentiation stages. The onset of GS synthesis occurred on depletion of L -glutamine and L -arginine. Soluble GS synthetase 1 and 2 peaks coincided with ‘diauxic’ lag phases; this supported the idea that a high growth rate is incompatible with GS synthetase formation.     

Dynamic portfolio choice: a simulation-and-regression approach

Simulation-and-regression algorithms have become a standard tool for solving dynamic programs in many areas, in particular financial engineering and computational economics. In virtually all cases, the regression is performed on the state variables, for example on current market prices. However, it is possible to regress on decision variables as well, and this opens up new possibilities. We present numerical evidence of the performance of such an algorithm, in the context of dynamic portfolio choices in discrete-time (and thus incomplete) markets. The problem is fundamentally the one considered in some recent papers that also use simulations and/or regressions: discrete time, multi-period reallocation, and maximization of terminal utility. In contrast to that literature, we regress on decision variables and we do not rely on Taylor expansions nor derivatives of the utility function. Only basic tools are used, bundled in a dynamic programming framework: simulations—which can be black-boxed—as a representation of exogenous state variable dynamics; regression surfaces, as non-anticipative representations of expected future utility; and nonlinear or quadratic optimization, to identify the best portfolio choice at each time step. The resulting approach is simple, highly flexible and offers good performance in time and precision.     

On‐Chip Self‐Assembly of a Smart Hybrid Nanocomposite for Antitumoral Applications

A hybrid nanocomposite comprised by porous silicon nanoparticles and a stimuli responsive polymeric material, polyethylene glycol‐block‐poly(L‐histidine), is spontaneously formed by nanoprecipitation in a flow‐focusing microfluidic chip. The nanocomposite presents a novel hybrid compound micelle structure with a great robustness for therapeutic applications. Therefore, the nanocomposite is developed and tested as a “smart” multistage drug delivery system (MDDS) in response to some of the current problems that cancer treatment presents. Based on the stimuli‐responsive behavior of the nanocomposite, a chemotherapeutic agent is successfully loaded into the nanosystem and released upon changes in the pH‐values. The nanocomposite demonstrates enhanced stability in plasma, narrow size distribution, improved surface smoothness, and high cytocompatibility. Furthermore, the nanocomposite presents reduced nanoparticle internalization by phagocytic macrophage cells and pH‐dependent cell growth inhibition capacity. Overall, the developed hybrid nanocomposite shows very promising features for its further development as a “smart” pH‐responsive MDDS.     

Sheep milk kefir sweetened with different sugars: Sensory acceptance and consumer emotion profiling

The objective of this study was to determine the sensory acceptance and emotional profile of sheep milk kefir sweetened with different sugars (demerara sugar, brown sugar, fructose, coconut sugar, and honey, 100 g/L). Consumers (n = 100) assessed sensory acceptance (appearance, aroma, taste, texture, and overall impression), and expressed their emotions (satisfied, active, loving, calm, comfortable, energetic, happy, healthy, refreshing, disgusted, worried, and upset). The emotions “satisfied,” “active,” “comfortable,” “energetic,” “healthy,” and “refreshing” were found between moderate and very high levels, indicating that they are important emotions for the characterization and sensory acceptance of kefir samples. The use of different sugars had no influence on the intensity of the emotions “calm,” “comfortable,” “happy,” or “disgusted,” and resulted in a greater sense of satisfaction. The use of demerara sugar or fructose did not alter the acceptance of the products or the intensity of emotions. The use of brown sugar decreased acceptance (taste, texture, and overall impression) and the intensity of the emotions “active,” “loving,” “energetic,” “healthy,” and “refreshing.” The use of coconut sugar decreased acceptance (appearance, aroma, and taste) and the intensity of the emotions “refreshing” and “upset.” The use of honey improved acceptance in appearance and aroma but reduced the intensity of the emotions “active,” “loving,” “energetic,” and “healthy.” Based on sensory data, it is recommended to use demerara sugar or fructose as a substitute for sucrose. In conclusion, the study of emotions can be used as an additional tool for obtaining data related to the sensory acceptance of products.     

Effect of probiotic Minas Frescal cheese on the volatile compound and metabolic profiles assessed by nuclear magnetic resonance spectroscopy and chemometric tools

This study aimed to evaluate the effect of Lacticaseibacillus casei 01 as a probiotic culture on the production of volatile organic compounds and metabolic profile of Minas Frescal cheese. Lactose (α-lactose and β-lactose), fatty acids (unsaturated and saturated), citric acid, tryptophan, and benzoic acid were the main compounds. Compared with the control cheese, probiotic cheese was characterized by the highest concentration of tryptophan and presented a higher number of volatile acids. The control cheese was characterized by the highest concentration of benzoic acid and fatty acids, resulting in a higher number of volatile alcohols and esters. No differences were observed for α-lactose, β-lactose, and citric acid contents. A clear separation of probiotic and control Minas Frescal cheese was obtained using ¹H nuclear magnetic resonance spectra, demonstrating that the addition of probiotic culture altered the metabolic profile of Minas Frescal cheese. Overall, the findings suggested that the addition of probiotic culture promoted the proteolysis in the fresh cheeses, decreased the lipolysis, and altered the volatile compounds. Furthermore, nuclear magnetic resonance spectroscopy coupled to chemometrics tools could be used to differentiate probiotic and conventional cheeses.