Regulation of bovine adipose tissue metabolism during lactation. 6. Cellularity and hormone-sensitive lipase activity as affected by genetic merit and energy intake

Subcutaneous adipose tissue cell size and density and hormone-sensitive lipase activity were determined during late pregnancy and first lactation in Holstein heifers. Animals were daughters of bulls with either +791 (high line) or +390 (low line) kg Predicted Difference (1974 base) for milk. Each line consumed either a 71%: 29% (high energy) or a 36%:63% (low energy) barley concentrate and alfalfa hay diet from 0 to 140 d lactation. Heifers fed the low energy ration ate 33% less NE1 and produced 20% less milk with 52.7% greater milk fat percentage during 28 to 140 d of lactation than heifers fed the high energy ration. Prepartum, animals of the high line had similar adipocyte volume and density to low line animals. However, during early lactation, high line animals had a greater decrease in volume and a larger increase in density than low line animals. There was a delayed recovery of volume in high line, compared with low line animals in late lactation. Adipocyte volume also was decreased by dietary energy restriction. Hormone-sensitive lipase activity was similar in both genetic lines prepartum and increased in all groups at 60 d postpartum. Animals of high line or fed low energy rations had increased activity per gram during early, but not later, lactation. Activity per cell and per milligram cytoplasm protein increased in all groups in early lactation and were highest in high line animals fed the low energy ration.     

Growth of gram-negative bacteria in dry cow secretion

Gram-negative bacteria (n = 192) isolated from infected bovine mammary glands were tested for growth in a pooled source of dry cow secretion. Growth of Klebsiella pneumoniae in dry cow secretion was greater than growth of Escherichia coli and Klebsiella oxytoca. Escherichia coli originating during the early dry period exhibited greater growth in dry cow secretion than those originating around calving or during lactation. Klebsiella pneumoniae growth did not differ with time of origin of intramammary infection. Escherichia coli, K. oxytoca, and K. pneumoniae growth in a synthetic medium was reduced by apolactoferrin plus Ig. Growth reduction was greatest for E. coli. Citrate reversed growth inhibition. The inhibitory properties of dry cow secretion for E. coli may contribute to the low number of naturally occurring intramammary infections originating during the early part of the dry period. Inhibitory properties of dry cow secretion are partially explained by lactoferrin acting in conjunction with antibody to prevent iron acquisition by many gram-negative bacteria.     

Protective effect of Staphylococcus chromogenes infection against Staphylococcus aureus infection in the lactating bovine mammary gland

The susceptibility of uninfected or Staphylococcus chromogenes-infected quarters to challenge with Staphylococcus aureus was measured. Seventeen S. chromogenes-infected quarters were challenged by infusion of S. aureus into the teat sinus; 47% (8 of 17) became infected and all 18 uninfected quarters challenged similarly with S. aureus became infected. No differences in daily milk yield were seen between uninfected quarters and S. chromogenes-infected quarters prior to S. aureus infusion. Postinfusion, milk yield for S. aureus-infected, S. chromogenes-infected, and S. chromogenes- and S. aureus-infected quarters differed. Somatic cell counts were elevated in S. chromogenes-infected quarters compared with uninfected quarters prior to S. aureus infusion. Somatic cell counts were not different between S. aureus- and S. chromogenes- and S. aureus-infected quarters postinfusion, but were different for S. chromogenes-infected quarters. Chloride concentrations in S. chromogenes- and S. aureus-infected quarters were different from either S. aureus-infected or S. chromogenes-infected quarters. Staphylococcus aureus colony forming units in quarters with preexisting S. chromogenes infections were lower than S. aureus colony-forming units in previously uninfected quarters. Possible protective mechanisms induced by S. chromogenes against superinfection by S. aureus are discussed.     

Effect of nordihydroguaiaretic acid on luteal phase length and oxytocin-induced release of prostaglandin F2 alpha in heifers

Two experiments were conducted to determine if intrauterine infusion of nordihydroguaiaretic acid, a lipoxygenase pathway inhibitor, would delay luteolysis (Experiment 1) and inhibit oxytocin-induced release of prostaglandin F2 alpha (as measured by the stable prostaglandin F2 alpha metabolite, 15-keto-13,14-dihydroprostaglandin F2 alpha) in plasma on d 16 (d 0 = estrus) of the estrous cycle (Experiment 2). Nordihydroguaiaretic acid (20 mg) or saline was infused twice daily into the uterus on d 14 to 23 (Experiment 1) or d 14 to 20 (Experiment 2) postestrus, respectively. In Experiment 1 and 2, mean concentration of progesterone was higher and luteolysis was delayed in nordihydroguaiaretic acid-infused heifers compared with saline-infused heifers. In Experiment 2, saline or oxytocin (100 IU, i.v.) was injected into each heifer on d 16 postestrus to stimulate the release of prostaglandin F2 alpha from the uterus. Mean concentration of 15-keto-13,14-dihydroprostaglandin F2 alpha increased within 1.5 h postinjection in heifers infused with saline, whereas concentration of 15-keto-13,14-dihydroprostaglandin F2 alpha in nordihydroguaiaretic acid-infused heifers did not increase within the same time period. Thus, nordihydroguaiaretic acid may inhibit both the lipoxygenase and cyclooxygenase pathways of arachidonic acid metabolism and therefore delay luteolysis.     

An ecological analysis of risk factors for postpartum disorders of Holstein-Friesian cows from thirty-two New York farms

Path analysis and multiple logistic-normal regression were used to model the interrelationships between stated herd management practices and herd 30-d postpartum incidence rates of dystocia, retained placenta, and clinical postpartum metritis, cystic ovary, milk fever, ketosis, left displaced abomasum, and mastitis. Management risk factors were obtained from a personal interview questionnaire. Data were from 2141 multiparous Holstein calvings from May 1981 through April 1982 in 32 commercial herds in the vicinity of Cornell University. Farms where the stated policy was to administer low calcium diets to the dry cows experienced more dystocia and retained placenta. More dystocia was experienced on farms where the stated policy was to administer extra vitamin D to dry cows, where dry cows were housed with the milking cows, or where calving occurred in maternity pens rather than stanchions. There was an increased likelihood of milk fever and left displaced abomasum on farms on which the stated policy was to lead feed. Farms where dairy farmers treated cases of retained placenta had more retained placenta than farms on which the treatment was done by the veterinarian. Farms reporting the practice of feeding the cows to increase their weight during the dry period had increased odds of ketosis.     

Digital video libraries and the Internet

Digital video libraries are positioned to be a major driving application for the Next Generation Internet Initiative. Likewise, the advancements in the communications infrastructure will be of great benefit to digital video libraries. This report investigates some of the recent technologies for handling video in digital video libraries and reviews the NGII's planned advancements in network communications technologies in order to examine their impact on deploying digital video libraries on the Internet     

一种适合VoIP电话应用的低成本PoE接口

本文介绍了Power Integrations(PI)开发的一种适用于电话上的以太网供电(PoE)分立接口电路,可帮助OEM厂商满足其设计和成本目标.     

1/f noise in large-area Hg<Subscript>1−x</Subscript>Cd<Subscript>x</Subscript>Te photodiodes

The 1/f noise in photovoltaic (PV) molecular-beam epitaxy (MBE)-grown Hg_1−xCd_xTe double-layer planar heterostructure (DLPH) large-area detectors is a critical noise component with the potential to limit sensitivity of the cross-track infrared sounder (CrIS) instrument. Therefore, an understanding of the origins and mechanisms of noise currents in these PV detectors is of great importance. Excess low-frequency noise has been measured on a number of 1000-μm-diameter active-area detectors of varying “quality” (i.e., having a wide range of I-V characteristics at 78 K). The 1/f noise was measured as a function of cut-off wavelength under illuminated conditions. For short-wave infrared (SWIR) detectors at 98 K, minimal 1/f noise was measured when the total current was dominated by diffusion with white noise spectral density in the mid-10⁻¹⁵A/Hz^1/2 range. For SWIR detectors dominated by other than diffusion current, the ratio, α, of the noise current in unit bandwidth i_n(f = 1 Hz, V_d = −60 mV, and Δf = 1 Hz) to dark current I_d(V_d = −60 mV) was α_SW-d = i_n/I_d ∼ 1 × 10⁻³. The SWIR detectors measured at 0 mV under illuminated conditions had median α_SW-P = i_n/I_ph ∼ 7 × 10⁻⁶. For mid-wave infrared (MWIR) detectors, α_MW-d = i_n/I_d ∼ 2 × 10⁻⁴, due to tunneling current contributions to the 1/f noise. Measurements on forty-nine 1000-μm-diameter MWIR detectors under illuminated conditions at 98 K and −60 mV bias resulted in α_MW-P = i_n/I_ph = 4.16 ± 1.69 × 10⁻⁶. A significant point to note is that the photo-induced noise spectra are nearly identical at 0 mV and 100 mV reverse bias, with a noise-current-to-photocurrent ratio, α_MW-P, in the mid 10⁻⁶ range. For long-wave infrared (LWIR) detectors measured at 78 K, the ratio, α_LW-d = i_n/I_d ∼ 6 × 10⁻⁶, for the best performers. The majority of the LWIR detectors exhibited α_LW-d on the order of 2 × 10⁻⁵. The photo-induced 1/f noise had α_LW-P = i_n/I_ph ∼ 5 × 10⁻⁶. The value of the noise-current-to-dark-current ratio, α appears to increase with increasing bandgap. It is not clear if this is due to different current mechanisms impacting 1/f noise performance. Measurements on detectors of different bandgaps are needed at temperatures where diffusion current is the dominant current. Excess low-frequency noise measurements made as a function of detector reverse bias indicate 1/f noise may result primarily from the dominant current mechanism at each particular bias. The 1/f noise was not a direct function of the applied bias.     

Far-infrared detector based on HgTe/HgCdTe superlattices

HgTe/Hg_0.05Cd_0.95Te superlattices (SLs) were grown on (112)B oriented Cd_0.96Zn_0.04 Te substrates using molecular beam epitaxy (MBE). The SLs, consisting of 100 periods of 80-Å-thick HgTe wells alternating with 77-Å-thick Hg_0.05Cd_0.95Te barriers, were designed to operate as detectors in the far-infrared (FIR) region. Infrared absorption spectroscopy, high-resolution transmission electron microscopy (TEM), Hall effect measurements, and x-ray diffraction were used to characterize the superlattice layers. A series of annealing experiments were initiated to quantify the temperature-dependent interdiffusion of the HgTe wells and Hg_0.05Cd_0.95Te barriers and consequently their degradation, which shifts the absorption edges of the SLs to higher energies, since a high-temperature ex situ anneal is normally required in order to produce the p-type material required for a photovoltaic detector. Results from infrared absorption spectroscopy, TEM, and Hall effect measurements for the annealed samples are presented. A FIR SLs single-element photoconductive (PC) device was designed and fabricated. Both material characterization and device testing have established the applicability of the HgTe/Hg_0.05Cd_0.95Te SLs for the FIR region.