Towards standardisation of proof load testing: pilot test on viaduct Zijlweg

Proof load tests of bridges can be very useful for structures with a lack of information, or for structures of which the effect of material degradation is difficult to assess. Contrary to diagnostic load testing, proof load testing is not well-defined in current standards in terms of required load and analysis of measurements. The risk related to the high loads used in proof load testing requires standardisation for these tests. The paper highlights important considerations for proof load testing that may lead to the development of guidelines in the Netherlands, by illustrating a pilot study on the viaduct Zijlweg in the Netherlands. This reinforced concrete bridge rates too low in shear. Topics of interest are the required load that the bridge has to withstand to be approved by the load test and the interpretation of the measurements during the test to avoid permanent damage to the structure. These measurements were compared to the stop criteria from existing codes for buildings, to examine if recommendations for the use with bridges can be formulated. The final result of the test on this case study is that the capacity of the viaduct is proven to be sufficient for shear and bending moment.     

Numerical and experimental study of conjugate heat transfer in a horizontal air cavity

The demand for general reduction of the energy consumption in civil engineering leads to more frequent use of insulating materials with air gaps or cavities. Heat transfer through a constructional part can be decreased by adding an air gap and low emissivity reflective foils to the structure. In the first part of this paper, the impacts of cavity thickness and inner surface emissivity on combined conduction, convection and radiation heat transfer was experimentally explored in the case of constructional part with a horizontal cavity subjected to constant downward heat flux. The heat flow meter Netzsch HFM 436 Lambda was used for steady-state measurements. Results suggest that the studied parameters seriously affect the combined heat transfer in the composed structure. In the second part the paper reports the numerical study of two-dimensional conjugate heat transfer in closed horizontal cavity having air as the intervening medium. Numerical models validated by related experimental results were performed to further investigate the effect of radiation heat transfer. It was found that in general, the total heat flux through the composed structure decreases with increasing air cavity thickness, which is significant especially when low emissivity inner surfaces are taking into account. The direction of heat flow (downward or upward heat flow) has a significant impact on the convection heat transfer. An important contribution from the present work is the analysis of the optimal thickness of the cavity at different boundary conditions. The optimal thickness of the enclosure with low emissivity surfaces is 16 mm when subjected to upward heat flux.     

Precise segmentation of the bulbar conjunctiva for hyperaemia images

Hyperaemia is an excess of blood in a tissue that causes the appearance of an unusual red hue in the affected area. It is a common occurrence in the bulbar conjunctiva, where it can be related to multiple pathologies, such as conjunctivitis or dry eye syndrome. Specialists grade hyperaemia by means of a tedious, subjective, non-repeatable and time-consuming process. These drawbacks can be solved with the automatisation of the process by means of image processing techniques. The automatic segmentation of the conjunctiva is an important part of the process, as it ensures the absence of noise in posterior stages of the methodology. However, there are several issues of illumination and focus in the input videos that difficult the process. In this work, several segmentation algorithms are proposed and compared in order to obtain an accurate location of the bulbar conjunctiva.     

Morphological co-processing unit for embedded devices

This paper focuses on the development of a fully programmable morphological coprocessor for embedded devices. It is a well-known fact that the majority of morphological processing operations are composed of a (potentially large) number of sequential elementary operators. At the same time, the industrial context induces a high demand on robustness and decision liability that makes the application even more demanding. Recent stationary platforms (PC, GPU, clusters) no more represent a computational bottleneck in real-time vision or image processing applications. However, in embedded solutions such applications still hit computational limits. The morphological co-processing unit (MCPU) replies to this demand. It assembles the previously published efficient dilation/erosion units with geodesic units and ALUs to support a larger collection of morphological operations, from a simple dilation to serial filters involving a geodesic reconstruction step. The coprocessor has been integrated into an FPGA platform running a server that is able to respond to client’s requests over the ethernet. The experimental performance of the MCPU measured on a wide set of operations brings as results in orders of magnitude better than another embedded platform, built around an ARM A9 quad-core processor.     

Flow and spawning habitat relationships for Dolly Varden: Understanding habitat–population dynamics in the Canadian Western Arctic

Northern form Dolly Varden (Salvelinus malma malma) have been designated as a species of Special Concern in Canada due to declines in population abundance and potential threats. Concern over detrimental effects of low flows on population abundance prompted research on how variability in discharge regimes influence habitat availability. Habitat suitability indices for prespawning and spawning adult anadromous Dolly Varden from two streams were integrated into a two‐dimensional hydrodynamic habitat model to assess the effect of flow variability on usable habitat. Regional hydrographs were used to identify an ecologically relevant range of flows that provided optimal spawning habitat for these populations and examine the relationship between abundance and discharge. Adults spawned in the tail end of pools at moderate water depths and water velocities, and used pebble‐ to cobble‐sized substrate for building redds; whereas, prespawning adults occupied deeper pools with moderate velocities and used cobble for cover. Model outputs showed that spawning habitat availability was optimized at flow rates between 1.6 and 3.0 m³/s and between 1.0 and 6.0 m³/s in Fish Hole Creek (FHC) and Little Fish Creek, respectively. A positive relationship between flows during the fall spawning period and abundance of the FHC population suggests that higher flows coinciding with optimal habitat availability may have contributed to positive recruitment. To strengthen and refine this habitat–population relationship for Dolly Varden in this area requires investigation of a broader suite of variables associated with environmental regimes and physical habitat in reaches used for spawning.     

Waste waters from the leachate of mushroom as vine foliar treatments: influence on grape volatile composition over two consecutive seasons

Edible mushrooms production presents a potential problem for the environment, due to the large amount of waste generated. These wastes contain nitrogenous matter, which could be used as biostimulant in plants. The aim of this work was to study the effect of waste waters, from the mushroom production process, applied at veraison and 1 week later to grapevine leaves, on grape volatile compounds over two vintages. The results evidenced that mushroom water treatment decreased the grape content of certain terpenoids, C₁₃ norisoprenoids, benzenoid compounds and esters, increasing C6 compounds content during the first season. Treated mushroom water application slightly affected grape volatile composition. This was the treatment that least affected the content of grape volatile composition. Few differences were found after the biostimulation during the second vintage. The season was the dominant factor of grape volatile content variation compared to the treatment and their interaction.     

Current and future experimental approaches in the study of grape and wine polyphenols interacting gut microbiota

Interactions between polyphenols and gut microbiota are indeed a major issue of current interest in food science research. Knowledge in this subject is progressing as the experimental procedures and analysis techniques do. The aim of this article is to critically review the more leading-edge approaches that have been applied so far in the study of the interactions between grape/wine polyphenols and gut microbiota. This is the case of in vitro dynamic gastrointestinal simulation models that try to mitigate the limitations of simple static models (batch culture fermentations). More complex approaches include the experimentation with animals (mice, rats, pigs, lambs and chicks) and nutritional intervention studies in humans. Main advantages and limitations as well as the most relevant findings achieved by each approach in the study of how grape/wine polyphenols can modulate the composition and/or functionality of gut microbiota, are detailed. Also, common findings obtained by the three approaches (in vitro, animal models and human nutritional interventions) such as the fact that the Firmicutes/Bacteroidetes ratio tends to decrease after the feed/intake/consumption of grape/wine polyphenols are highlighted. Additionally, a nematode (Caenorhabditis elegans) model, previously used for investigating the mechanisms of processes such as aging, neurodegeneration, oxidative stress and inflammation, is presented as an emerging approach for the study of polyphenols interacting gut microbiota. © 2020 Society of Chemical Industry     

The effect of human chorionic gonadotrophin on the expression of progesterone receptors in human luteal cells in vivo and in vitro

The human corpus luteum expresses genomic progesterone receptors (PRs) suggesting that progesterone may have an autocrine or paracrine role in luteal function. We hypothesised that the reduction in luteal PR reported in the late-luteal phase augmented progesterone withdrawal and had a role in luteolysis. We therefore tested the hypothesis that luteal rescue with human chorionic gonadotrophin (hCG) would maintain PR expression. PR was immunolocalised to different cell types in human corpora lutea (n = 35) from different stages of the luteal phase and after luteal rescue with exogenous hCG. There was no change in the staining intensity of theca-lutein cell or stromal cell PR throughout the luteal phase or after luteal rescue. In the late-luteal phase, granulosa-lutein cell PR immunostaining was reduced (P < 0.05) but the trend to reduction was also seen after luteal rescue with hCG (P = 0.055). To further investigate the effect of hCG on granulosa-lutein cell PR expression, an in vitro model system of cultured human luteinised granulosa cells was studied. Cells were cultured for 12-13 days exposed to different patterns of hCG and aminoglutethamide to manipulate progesterone secretion (P < 0.0001). Expression of PR A/B and PR B isoforms was examined by quantitative real-time RT-PCR. PR A/B mRNA was lower (P < 0.05) after 11-13 days of culture than after 7 days of culture. This reduction could not be prevented by hCG in the presence (P < 0.05) or absence (P < 0.05) of stimulated progesterone secretion. The expression of PR B mRNA showed a similar pattern (P = 0.054). Simulated early pregnancy in vivo and hCG treatment of luteinised granulosa cells in vitro did not appear to prevent the down-regulation of PR seen during luteolysis.