The effects of insulin on transport and metabolism of glucose in skeletal muscle from hyperthyroid and hypothyroid rats

The effects of insulin on the rates of glucose disposal were studied in soleus muscles isolated from hyper- or hypothyroid rats. Treatment with triiodothyronine for 5 or 10 days decreased the sensitivity of glycogen synthesis but increased the sensitivity of lactate formation to insulin. The sensitivity of 3-O methylglucose to insulin was increased only after 10 days of treatment and was accompanied by an increase in the sensitivity of 2-deoxyglucose phosphorylation; however, 2-deoxyglucose and glucose 6-phosphate in response to insulin remained unaltered. In hypothyroidism, insulin-stimulated rates of 3-O-methylglucose transport and 2-deoxyglucose phosphorylation were decreased; however, at basal levels of insulin, 3-O-methylglucose transport was increased, while 2-deoxyglucose phosphorylation was normal. In these muscles, the sensitivity of lactate formation to insulin was decreased; this defect was improved after incubation of the muscles with prostaglandin E2. The results suggest: (a) in hyperthyroidism, insulin-stimulated rates of glucose utilization in muscle to form lactate are increased mainly because of a decrease in glycogen synthesis; when hyperthyroidism progresses in severity, increases in the sensitivity of glucose transport to insulin and in the activity of hexokinase may also be involved; (b) in hypothyroidism, the decrease in insulin-stimulated rates of glucose utilization is caused by decreased rates of glycolysis; (c) prostaglandins may be involved in the changes in sensitivity of glucose utilization to insulin observed in muscle in altered thyroid states.     

A Lagrangian approach for vorticity intensification in swirling rings

The Lagrangian approach, adopted in vortex methods for incompressible Euler flows, is reconsidered here as the most appropriate technique to track the dynamical evolution of organized vorticity structures in free space. We analyze in this paper a specific initial condition, the swirling ring with azimuthal perturbations, from which originate vortex dynamics events leading to a localized but strong vorticity intensification. The aim is to understand the possible development of a finite time singularity, in the context of inviscid flows, and its relevance for the study of intermittency, as one of the most challenging aspects of turbulence.     

Type 1 astrocytes influence luteinizing hormone-releasing hormone release from the hypothalamic cell line GT1-1: is transforming growth factor-beta the principle involved?

The possible existence of a humoral communication between glial cells and LHRH-secreting neurons has been studied using the LHRH-secreting GT1-1 cell line and type 1 astrocytes. Two different designs have been adopted: 1) GT1-1 cells were coincubated with purified cultures of type 1 rat astrocytes, and 2) GT1-1 cells were exposed to the conditioned medium (CM) in which type 1 rat astrocytes had been grown for 24 h. LHRH was measured by RIA in the medium of the GT1-1 cell cultures at different time intervals. The data show that short periods (1, 3, and 6 h) of either coculture or exposure to previously frozen CM significantly increase the release of LHRH from the GT1-1 cells. However, more prolonged times of coculture (e.g. 2 and 5 days) or exposure to CM (e.g. 48 h) induce a significant decrease in the amount of LHRH in the medium. The stimulatory effect on LHRH release appears to be specific for type 1 astrocytes (either cortical or hypothalamic), because neither the CM of oligodendrocytes nor the CM of LNCaP cells (a cell line derived from a human prostatic cancer) possess stimulating activities. Heating the type 1 astrocyte-CM to 100 C for 10 min does not eliminate the ability of the CM to significantly increase the release of LHRH from GT1-1 cells at 1, 3, and 6 h. Because of the opposite effects encountered in the short and long term experiments, it was hypothesized that the CM might contain, in addition to LHRH-releasing principle(s), LHRH-degrading properties. Known amounts of standard LHRH were then added to type 1 astrocyte-CM, either untreated or submitted to heating at 100 C for 10 min. The amount of LHRH added to untreated CM decreases progressively; on the contrary, the amount of LHRH added to heated CM remains unchanged. These results confirm that one or more heat-sensitive enzymes able to degrade LHRH may be present in the type 1 astrocyte-CM. As previously mentioned, the experiments reported so far were performed using type 1 astrocyte-CM that had been kept frozen for various periods of time, before being tested for its LHRH-releasing activity. Surprisingly, fresh CM proves to be inactive, whereas heated CM is effective; this suggests that the factor involved might be activated by the two opposite experimental procedures.(ABSTRACT TRUNCATED AT 400 WORDS)     

From a boundary integral formulation to a vortex method for viscous flows

For the numerical solution of flow problems past a solid body it is worth to consider boundary integral techniques for their inherent capability to manage efficiently the far-field boundary conditions as well as the approximation of the solid body contour. However, for the analysis of large Reynolds number flows, of major interest in the applications, several computational difficulties appear when using the integral representation for the velocity or for the vorticity field in its classical form with interpolating functions (BEM). In particular, the evaluation of the volume integrals is a serious drawback while the steepness of their kernel introduces artificial diffusion in the calculation. To satisfy the opposite requirements of the advective and of the diffusive part of the Navier-Stokes equations, we adopt an operator splitting scheme according to the Chorin-Marsden product formula (Chorin et al. 1978), together with a proper vorticity generation scheme at the solid boundary. A solution procedure based on the approximation of the vorticity field by a finite number of point vortices (PVM) follows as a natural evolution of the boundary integral formulation.The numerical results given by the two methods for the merging of two like-signed vortices in free space reveal the excessive numerical diffusion of BEM. The better accuracy of PVM is also established through the evaluation of some first integrals of motion. Several results are also reported for flows in presence of solid boundaries where the vorticity generation is crucial. In this case accurate solutions are only obtained with PVM, while BEM is even less satisfactory than in free space. Finally, the proposed vortex-like method (PVM) is tested on the classical problem of the wake behind a cylinder, in comparison with other well established techniques.This work was partly supported by the Italian Ministry for Scientific Research through a MURST grant and by C.N.R. through Progetto Finalizzato Trasporti II.     

In‐Line Monitoring and Control of Conversion and Weight‐Average Molecular Weight of Polyurethanes in Solution Step‐Growth Polymerization Based on Near Infrared Spectroscopy and Torquemetry

Summary: It is well known that the weight‐average molecular weight ( ) is strictly dependent on conversion in step‐growth polymerizations performed in batch and that the is very sensitive to impurities and molar imbalance. This makes the work of controlling a non trivial job. In this paper a new methodology is introduced for in‐line monitoring and control of conversion and of polyurethanes produced in solution step‐growth polymerizations, based on near‐infrared spectroscopy (NIRS) and torquemetry. A calibration model based on the PLS method is obtained and validated for monomer conversion, while the weight‐average molecular weight is monitored indirectly with the relative shear signal provided by the agitator. Control procedures are then proposed and implemented experimentally to avoid gelation and allow for maximization of . The proposed monitoring and control procedures can also be applied to other step growth polymerizations.

Proposed control scheme.     

In vitro evaluation of the microbial contamination on new toothbrushes: a preliminary study

Objective: The presence and survival of microorganisms on toothbrush bristles might play a role on the etiology of oral infections. The aim of this in vitro study was to evaluate the presence of bacterial contamination on new toothbrushes before oral contact. Materials and methods: Forty toothbrushes from five different manufacturers were used in this experimental study. Each manufacturer was divided according to conventional local of obtaining: industry, drugstore, market, and perfumery. The toothbrush heads were completely immersed into tubes containing 5.0 mL of sterile peptonated water (dilution 1:10). A group of eight tubes containing the sterile solution was used as control. After 21 days of anaerobic incubation, occurrence of contamination was visually evaluated and confirmed by light microscopy. Results: Bacterial growth in the medium, indicative of bristles contamination, was found in a total of 19 out of 40 samples (47.5%) evaluated: 6 out of 14 samples (42.85%) from industry group, 4 out of 8 samples (50.0%) from drugstore, 5 out of 10 samples (50.0%) from market, and 4 out of 8 samples (50.0%) from perfumery. Only the toothbrushes with bristles coated with chlorhexidine did not show contamination. The Gram‐negative sporulating bacilli were the most prevalent form recovered. Conclusions: Except for chlorhexidine group, bacterial growth was observed in all groups evaluated irrespective local of obtaining. Microsc. Res. Tech., 2012. © 2011 Wiley Periodicals, Inc.     

On the intersonic crack propagation in an orthotropic medium

Motivaded by recent theoretical studies the elastodynamic response of an orthotropic material with a semi-infinite line crack, which propagates intersonically. is revisited through an approach which differs from those used in previous studies. The near tip stress and displacement fields are obtained for Mode I and Mode II of steady state crack propagation. The strain energy release rate analysis confirms that the Mode I is physically impossible due to the order of stress singularity, which is larger then one half. For Model II the order of stress is less than one half and it is shown that a steady state intersonic propagation is allowed only for a particular crack tip velocity which is a function of the material orthotropy.     

Improving optimum-path forest learning using bag-of-classifiers and confidence measures

Pattern Analysis and Applications - Machine learning techniques have been actively pursued in the last years, mainly due to the great number of applications that make use of some sort of...