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51.
Karolina Ossowska Agata Motyka-Pomagruk Natalia Kaczyska Agnieszka Kowalczyk Wojciech Sledz Ewa Lojkowska Zbigniew Kaczyski 《International journal of molecular sciences》2022,23(4)
Pectobacterium parmentieri is a pectinolytic plant pathogenic bacterium causing high economic losses of cultivated plants. The highly devastating potential of this phytopathogen results from the efficient production of plant cell wall-degrading enzymes, i.e., pectinases, cellulases and proteases, in addition to the impact of accessory virulence factors such as motility, siderophores, biofilm and lipopolysaccharide (LPS). LPS belongs to pathogen-associated molecular patterns (PAMPs) and plays an important role in plant colonization and interaction with the defense systems of the host. Therefore, we decided to investigate the heterogeneity of O-polysaccharides (OPS) of LPS of different strains of P. parmentieri, in search of an association between the selected genomic and phenotypic features of the strains that share an identical structure of the OPS molecule. In the current study, OPS were isolated from the LPS of two P. parmentieri strains obtained either in Finland in the 1980s (SCC3193) or in Poland in 2013 (IFB5432). The purified polysaccharides were analyzed by utilizing 1D and 2D NMR spectroscopy (1H, DQF-COSY, TOCSY, ROESY, HSQC, HSQC-TOCSY and HMBC) in addition to chemical methods. Sugar and methylation analyses of native polysaccharides, absolute configuration assignment of constituent monosaccharides and NMR spectroscopy data revealed that these two P. parmentieri strains isolated in different countries possess the same structure of OPS with a very rare residue of 5,7-diamino-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (pseudaminic acid) substituted in the position C-8: →3)-β-d-Galf-(1→3)-α-d-Galp-(1→8)-β-Pse4Ac5Ac7Ac-(2→6)-α-d-Glcp-(1→6)-β-d-Glcp-(1→. The previous study indicated that three other P. parmentieri strains, namely IFB5427, IFB5408 and IFB5443, exhibit a different OPS molecule than SCC3193 and IFB5432. The conducted biodiversity-oriented assays revealed that the P. parmentieri IFB5427 and IFB5408 strains possessing the same OPS structure yielded the highest genome-wide similarity, according to average nucleotide identity analyses, in addition to the greatest ability to macerate chicory tissue among the studied P. parmentieri strains. The current research demonstrated a novel OPS structure, characteristic of at least two P. parmentieri strains (SCC3193 and IFB5432), and discussed the observed heterogenicity in the OPS of P. parmentieri in a broad genomic and phenotype-related context. 相似文献
52.
Celina Kruszniewska-Rajs Barbara Strzaka-Mrozik Magdalena Kimsa-Dudek Agnieszka Synowiec-Wojtarowicz Elwira Chrobak Ewa Bbenek Stanisaw Boryczka Stanisaw Guszek Joanna Magdalena Gola 《International journal of molecular sciences》2022,23(5)
Betulin and its derivatives, 28-propyne derivative EB5 and 29-diethyl phosphonate analog ECH147, are promising compounds in anti-tumor activity studies. However, their effect on kidney cells has not yet been studied. The study aimed to determine whether betulin and its derivatives—EB5 and ECH147—influence the viability and oxidative status of human renal proximal tubule epithelial cells (RPTECs). The total antioxidant capacity of cells (TEAC), lipid peroxidation product malondialdehyde (MDA) level, and activity of antioxidant enzymes (SOD, CAT, and GPX) were evaluated. Additionally, the mRNA level of genes encoding antioxidant enzymes was assessed. Cisplatin and 5-fluorouracil were used as reference substances. Betulin and its derivatives affected the viability and antioxidant systems of RPTECs. Betulin strongly reduced TEAC in a concentration-dependent manner. All tested compounds caused an increase in MDA levels. The activity of SOD, CAT, and GPX, and the mRNA profiles of genes encoding antioxidant enzymes depended on the tested compound and its concentration. Betulin showed an cisplatin-like effect, indicating its nephrotoxic potential. Betulin derivatives EB5 and ECH147 showed different impacts on the antioxidant system, which gives hope that these compounds will not cause severe consequences for the kidneys in vivo. 相似文献
53.
Emilia Pers-Kamczyc Ewa Mderek Jacek Kamczyc 《International journal of molecular sciences》2022,23(6)
Although seed quality and quantity, as well as reproductive performance are important life history stages of plants, little is known about the reproductive responses of trees to environmental changes such as increased anthropogenic deposition of nitrogen (N) and phosphorus (P). Dioecious plants are good models with which to test the environmental impact on female or male reproductive responses individually. We analysed effects of different long-term nutritional availability on the reproductive performance of two dioecious species (Taxus baccata L. and Juniperus communis L.) characterised by different life histories. By using pot experiments with vegetatively propagated plants grown in different fertilisation conditions, we observed an increase in plant growth and strobili production but a decrease in seed efficiency. Seeds produced by fertilised plants had greater seed mass. Fertiliser addition did not change C or N content nor the C/N ratio of T. baccata seeds, but increased N content and the N/P ratio; however, it did lower the C/N ratio in J. communis. Fertilisation did not change the metabolite profile in T. baccata but 18 metabolites were changed in J. communis. The study revealed new links between species life history, environmental changes, and reproduction. The findings imply that future environmental conditions may alter both seed productivity, and quality, as well as plant reproductive behaviour. 相似文献
54.
Izabela Barya Ewa Stycze-Binkowska Elbieta Puciennik Katarzyna Kola Andrzej K. Bednarek 《International journal of molecular sciences》2022,23(6)
Recent reports indicate that the hypoxia-induced factor (HIF1α) and the Warburg effect play an initiating role in glucotoxicity, which underlies disorders in metabolic diseases. WWOX has been identified as a HIF1α regulator. WWOX downregulation leads to an increased expression of HIF1α target genes encoding glucose transporters and glycolysis’ enzymes. It has been proven in the normoglycemic mice cells and in gestational diabetes patients. The aim of the study was to determine WWOX’s role in glucose metabolism regulation in hyperglycemia and hypoxia to confirm its importance in the development of metabolic disorders. For this purpose, the WWOX gene was silenced in human normal fibroblasts, and then cells were cultured under different sugar and oxygen levels. Thereafter, it was investigated how WWOX silencing alters the genes and proteins expression profile of glucose transporters and glycolysis pathway enzymes, and their activity. In normoxia normoglycemia, higher glycolysis genes expression, their activity, and the lactate concentration were observed in WWOX KO fibroblasts in comparison to control cells. In normoxia hyperglycemia, it was observed a decrease of insulin-dependent glucose uptake and a further increase of lactate. It likely intensifies hyperglycemia condition, which deepen the glucose toxic effect. Then, in hypoxia hyperglycemia, WWOX KO caused weaker glucose uptake and elevated lactate production. In conclusion, the WWOX/HIF1A axis downregulation alters glucose metabolism and probably predispose to metabolic disorders. 相似文献
55.
56.
Agnieszka Łącz Łukasz Łańcucki Radosław Lach Bartosz Kamecki Ewa Drożdż 《International Journal of Hydrogen Energy》2018,43(18):8999-9005
Series of single-phase materials with assumed formula SrTi1?xCrxO3 (where x = 0, 1, 4, 6 mol.%) were obtained by sol-gel method. The structure and microstructure of materials were characterised by X-ray diffraction and scanning electron microscopy methods. Moreover, the study of electrical properties and evaluation of chemical stability in CO2/H2O atmosphere was performed by electrochemical impedance spectroscopy and thermogravimery methods, respectively. The possibility of participation of Cr-doped strontium titanate in oxidation–reduction processes was analysed by temperature-programed reduction (TPR) and temperature-programed oxidation (TPOx) measurements. The changes of lattice parameters together with XPS analysis, the Seebeck coefficient measurements results and TPR profiles obtained for SrTi1?xCrxO3 materials prove the presence of chromium on +3 and +6 oxidation stages. Thus, chromium can be treated as both acceptor- and donor-type dopant in the SrTiO3 structure. The Cr3+/Cr6+ ratio strongly affects the electrical properties, as the change of conduction mechanism was observed. The results of performed stability test clearly indicate that incorporation of chromium into SrTiO3 structure results with decrease of chemical stability in CO2 atmosphere. 相似文献
57.
Grażyna Budryn Dorota Żyżelewicz Ewa Nebesny Joanna Oracz Wiesława Krysiak 《Food research international (Ottawa, Ont.)》2013,50(1):149-160
This study investigated the influence of antioxidants of green tea aqueous extract (GTE) and green coffee aqueous extract (GCE) in concentrations of 0.25, 0.5 and 1 g·100 g? 1 on the nutritional properties of fine yeast pastry fried products such as donuts. Yeast donuts were made according to the traditional processing technology. In fried donuts physical properties (water activity, pH, hardness and color with the use of instrumental methods) as well as sensory properties were established. Moreover, quality of fat extracted from donuts (composition of fatty acids and peroxide value), content of acrylamide, composition of polyphenols and antioxidant activity were analyzed. Obtained extracts of GTE and GCE contained 26 and 41% of polyphenols, respectively, and the degree of polyphenol degradation during production of donuts was less when the dilution in dough was greater. Both tests indicated that the highest antioxidant activity had donuts with the highest analyzed addition of extract, however depending on the applied test or GTE or GCE had better antioxidant properties. Fatty acid composition was the most beneficial when the addition of GTE and GCE was on a level of 0.25 and 0.5 g·100 g? 1, respectively. In fried donuts relatively low amounts of acrylamide were obtained, amounting to 18–46 μg·kg? 1. Only the lowest addition of GTE and the highest addition of GCE caused an increase of acrylamide content in donuts, while all other levels of added extracts caused a decrease in the content of formed acrylamide. 相似文献
58.
Ewa Bogel-?ukasik Susana Santos Manuel Nunes da Ponte 《The Journal of Supercritical Fluids》2010,54(2):210-12548
Ionic liquids as coated catalysts or additives tremendously alter the selectivity pattern of the heterogeneous solid catalyst in the selective hydrogenation of limonene. The conventional monometallic ruthenium over alumina catalyst combined with an ionic liquid enables the one-pot synthesis of the intermediate p-menthene through limonene hydrogenation in supercritical carbon dioxide. Among eight screened imidazolium ILs, [C10mim]NTf2 was employed as additive, or as the coating agent of ruthenium catalyst in the reaction under supercritical conditions.The coating of the heterogeneous catalyst allows the selective production of p-menthene and increases the conversion level of limonene (>99%) compared to the conversion of limonene in the reaction carried out in the presence of an ionic liquid as an additive. Results of the catalyst recycling indicate that there is no depletion of catalyst reactivity even after four successive cycles of operation under the studied reaction conditions. Further hydrogenation of p-menthene is strongly inhibited by employing an ionic liquid. The solubility of limonene or p-menthene in an ionic liquid governs the selective hydrogenation of limonene towards p-menthene. 相似文献
59.
Large-scale applications can be expressed as a set of tasks with data dependencies between them, also known as application
workflows. Due to the scale and data processing requirements of these applications, they require Grid computing and storage
resources. So far, the focus has been on developing easy to use interfaces for composing these workflows and finding an optimal
mapping of tasks in the workflow to the Grid resources in order to minimize the completion time of the application. After
this mapping is done, a workflow execution engine is required to run the workflow over the mapped resources. In this paper,
we show that the performance of the workflow execution engine in executing the workflow can also be a critical factor in determining
the workflow completion time. Using Condor as the workflow execution engine, we examine the various factors that affect the
completion time of a fine granularity astronomy workflow. We show that changing the system parameters that influence these
factors and restructuring the workflow can drastically reduce the completion time of this class of workflows. We also examine
the effect on the optimizations developed for the astronomy application on a coarser granularity biology application. We were
able to reduce the completion time of the Montage and the Tomography application workflows by 90% and 50%, respectively. 相似文献
60.
Ewa Dryzek 《Journal of Materials Science》2003,38(18):3755-3763
The paper presents the experimental results of positron annihilation measurements of the subsurface zones in aluminum and aluminum alloy exposed to the indentation of the small steel ball or blasting by the silicon carbide particles. The measurements of the Doppler broadening of the annihilation line and positron lifetime (PL) enabled the depth profile of open volume defects induced by indentation and blasting processes to be obtained. The coincidence of the von Mises criterion for yield with the onset of the increase of the defect concentration at certain depth was detected. It was established that the defects created in the pure aluminum and the aluminum alloy detected by positrons are different. However, the kinds of induced defects do not change with depth. The PL measurements indicate the presence of vacancy clusters in samples exposed to blasting. The vacancy clusters created in the aluminum alloy are larger than in the pure aluminum. 相似文献