Versatile and Easily Designable Polyester-Laser Toner Interfaces for Site-Oriented Adsorption of Antibodies

Laser toners appear as attractive materials for barriers and easily laminated interphases for Lab-on-a-Foil microfluidics, due to the excellent adhesion to paper and various membranes or foils. This work shows for the first time a comprehensive study on the adsorption of antibodies on toner-covered poly(ethylene terephthalate) (PET@toner) substrates, together with assessment of such platforms in rapid prototyping of disposable microdevices and microarrays for immunodiagnostics. In the framework of presented research, the surface properties and antibody binding capacity of PET substrates with varying levels of toner coverage (0–100%) were characterized in detail. It was proven that polystyrene-acrylate copolymer-based toner offers higher antibody adsorption efficiency compared with unmodified polystyrene and PET as well as faster adsorption kinetics. Comparative studies of the influence of pH on the effectiveness of antibodies immobilization as well as measurements of surface ζ-potential of PET, toner, and polystyrene confirmed the dominant role of hydrophobic interactions in adsorption mechanism. The applicability of PET@toner substrates as removable masks for protection of foil against permanent hydrophilization was also shown. It opens up the possibility of precise tuning of wettability and antibody binding capacity. Therefore, PET@toner foils are presented as useful platforms in the construction of immunoarrays or components of microfluidic systems.     

Amorphous Inclusion Complexes: Molecular Interactions of Hesperidin and Hesperetin with HP-Β-CD and Their Biological Effects

This study aimed at obtaining hesperidin (Hed) and hesperetin (Het) systems with HP-β-CD by means of the solvent evaporation method. The produced systems were identified using infrared spectroscopy (FT-IR), X-ray powder diffraction (XRPD), and differential scanning calorimetry (DSC). Moreover, in silico docking and molecular dynamics studies were performed to assess the most preferable site of interactions between tested compounds and HP-β-CD. The changes of physicochemical properties (solubility, dissolution rate, and permeability) were determined chromatographically. The impact of modification on biological activity was tested in an antioxidant study as well as with regards to inhibition of enzymes important in pathogenesis of neurodegenerative diseases. The results indicated improvement in solubility over 1000 and 2000 times for Hed and Het, respectively. Permeability studies revealed that Hed has difficulties in crossing biological membranes, in contrast with Het, which can be considered to be well absorbed. The improved physicochemical properties influenced the biological activity in a positive manner by the increase in inhibitory activity on the DPPH radical and cholinoesterases. To conclude the use of HP-β-CD as a carrier in the formation of an amorphous inclusion complex seems to be a promising approach to improve the biological activity and bioavailability of Hed and Het.     

Cell Death by Entosis: Triggers,Molecular Mechanisms and Clinical Significance

Entosis—a homotypic insertion of one cell into another, resulting in a death of the invading cell—has been described in many reports, but crucial aspects of its molecular mechanisms and clinical significance still remain controversial. While actomyosin contractility of the invading cell is very well established as a driving force in the initial phase, and autophagy induced in the outer cell is determined as the main mechanism of degradation of the inner cell, many details remain unresolved. The multitude of triggering factors and crisscrossing molecular pathways described in entosis regulation make interpretations difficult. The question of the physiological role of entosis also remains unanswered. In this review, we summarize the knowledge of molecular mechanisms and clinical data concerning entosis accumulated so far, highlighting both coherent explanations and controversies.     

Trichoderma: The Current Status of Its Application in Agriculture for the Biocontrol of Fungal Phytopathogens and Stimulation of Plant Growth

Rhizosphere filamentous fungi of the genus Trichoderma, a dominant component of various soil ecosystem mycobiomes, are characterized by the ability to colonize plant roots. Detailed knowledge of the properties of Trichoderma, including metabolic activity and the type of interaction with plants and other microorganisms, can ensure its effective use in agriculture. The growing interest in the application of Trichoderma results from their direct and indirect biocontrol potential against a wide range of soil phytopathogens. They act through various complex mechanisms, such as mycoparasitism, the degradation of pathogen cell walls, competition for nutrients and space, and induction of plant resistance. With the constant exposure of plants to a variety of pathogens, especially filamentous fungi, and the increased resistance of pathogens to chemical pesticides, the main challenge is to develop biological protection alternatives. Among non-pathogenic microorganisms, Trichoderma seems to be the best candidate for use in green technologies due to its wide biofertilization and biostimulatory potential. Most of the species from the genus Trichoderma belong to the plant growth-promoting fungi that produce phytohormones and the 1-aminocyclopropane-1-carboxylate (ACC) deaminase enzyme. In the present review, the current status of Trichoderma is gathered, which is especially relevant in plant growth stimulation and the biocontrol of fungal phytopathogens.     

Electrochemical discolouration and degradation of reactive dichlorotriazine dyes: reaction pathways

Results of electrochemical oxidation of two reactive dichlorotriazine dyes: Reactive Red 2 and Reactive Blue 81, are presented in this paper. Two electrode materials: Ti/TiO₂(70%)–RuO₂(30%) and carbon felt, were applied in the investigations as anodes. Voltammetric measurements show that Reactive Blue 81 electrooxidation proceeds easier and with higher rate than electrooxidation of Reactive Red 2. Both dyes are oxidised irreversibly in at least one electrode step before oxygen evolution starts at the electrode. Effectiveness of electrochemical oxidation under potentiostatic conditions achieved for Reactive Blue 81 was higher than for Reactive Red 2, with application of a carbon felt anode. Pulse radiolysis measurements prove addition of ^•OH radical to the dye molecule and formation of cyclohexadienyl and naphthoxyl radicals. Results of voltammetric analysis, pulse radiolysis measurements and GC–MS identification of intermediate products suggest two possible pathways of the dyes electrochemical oxidation.     

UV stability of polymeric binder films used in waterborne facade paints

The UV-stability study of four polymeric binders for water-borne paints, based on acrylic, styrene-acrylic, vinyl acetate and vinyl acetate-butyl acrylate (co)polymers is described. To the best of our knowledge the UV stability of this set of binders relevant to decorative industry is systematically compared for the first time. Thin films obtained from the respective latexes were irradiated with UV light (λ ≥ 275 nm) and analysed using electrokinetic potential (ζ-potential), FTIR ATR spectroscopy, SEM microscopy and differential scanning calorimetry (DSC). The films obtained from different latexes displayed distinct changes in their surface composition and response to UV-irradiation. Among the studied compositions, the vinyl acetate-based ones seem to be the least prone to UV-degradation in the absence of other components typically present in paint formulations. The analytical techniques employed in this study are shown to be complementary and provide information on different regions of the surface layer, depending on their effective probing depth. Especially, a combination of FTIR and ζ-potential measurements is shown useful to clarify the nature of the carbonyl groups formed during photodegradation.     

Lactic Acid Fermentation of Edible Mushrooms: Tradition,Technology, Current State of Research: A Review

Pickling is one of the methods for preserving food. However, this term may refer to both types of products, that is, to those subjected to lactic acid fermentation and to marinated ones (acidified) that are usually produced by the addition of acetic acid. Various raw materials are subjected to lactic acid fermentation (vegetable and animal origin), which yields food products with high nutritional and dietary value. In many regions of the world, the process of lactic fermentation is also traditionally used to preserve fruiting bodies of edible mushrooms. Mushrooms are appreciated for their organoleptic qualities as well as the presence of many different bioactive substances exhibiting healing and health‐promoting properties. This article reviews the literature related to the use of lactic fermentation in the process of mushroom preservation. Particular attention has been paid to the aspects of the technological process and its impact on the quality and suitability of the final products. Moreover, research results concerning the influence of lactic fermentation on chemical and physical changes in fruiting bodies of edible fungi are also presented.     

Porous carbon nanosheet with high surface area derived from waste poly(ethylene terephthalate) for supercapacitor applications

Converting waste plastics into valuable carbon materials has obtained increasing attention. In addition, carbon materials have shown to be the ideal electrode materials for double-layer supercapacitors owing to their large specific surface area, high electrical conductivity, and stable physicochemical properties. Herein, an easily operated approach is established to efficiently convert waste poly(ethylene terephthalate) beverage bottles into porous carbon nanosheet (PCNS) through the combined processes of catalytic carbonization and KOH activation. PCNS features an ultrahigh specific surface area (2236 m² g⁻¹), hierarchically porous architecture, and a large pore volume (3.0 cm³ g⁻¹). Such excellent physicochemical properties conjointly contribute to the outstanding supercapacitive performance: 169 F g⁻¹ (6 M KOH) and 135 F g⁻¹ (1 M Na₂SO₄). Furthermore, PCNS shows a high capacitance of 121 F g⁻¹ and a corresponding energy density of 30.6 Wh kg⁻¹ at 0.2 A g⁻¹ in the electrolyte of 1 M TEATFB/PC. When the current density increases to 10 A g⁻¹, the capacitance remains at 95 F g⁻¹, indicating the extraordinary rate capability. This work not only proposes a facile approach to synthesize PCNS for supercapacitors, but also puts forward a potential sustainable way to recycle waste plastics and further hopefully mitigates the waste plastics-related environmental issues. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137, 48338.     

Design and Development of a New Type of Hybrid PLGA/Lipid Nanoparticle as an Ursolic Acid Delivery System against Pancreatic Ductal Adenocarcinoma Cells

Despite many attempts, trials, and treatment procedures, pancreatic ductal adenocarcinoma (PDAC) still ranks among the most deadly and treatment-resistant types of cancer. Hence, there is still an urgent need to develop new molecules, drugs, and therapeutic methods against PDAC. Naturally derived compounds, such as pentacyclic terpenoids, have gained attention because of their high cytotoxic activity toward pancreatic cancer cells. Ursolic acid (UA), as an example, possesses a wide anticancer activity spectrum and can potentially be a good candidate for anti-PDAC therapy. However, due to its minimal water solubility, it is necessary to prepare an optimal nano-sized vehicle to overcome the low bioavailability issue. Poly(lactic-co-glycolic acid) (PLGA) polymeric nanocarriers seem to be an essential tool for ursolic acid delivery and can overcome the lack of biological activity observed after being incorporated within liposomes. PLGA modification, with the addition of PEGylated phospholipids forming the lipid shell around the polymeric core, can provide additional beneficial properties to the designed nanocarrier. We prepared UA-loaded hybrid PLGA/lipid nanoparticles using a nanoprecipitation method and subsequently performed an MTT cytotoxicity assay for AsPC-1 and BxPC-3 cells and determined the hemolytic effect on human erythrocytes with transmission electron microscopic (TEM) visualization of the nanoparticles and their cellular uptake. Hybrid UA-loaded lipid nanoparticles were also examined in terms of their stability, coating dynamics, and ursolic acid loading. We established innovative and repeatable preparation procedures for novel hybrid nanoparticles and obtained biologically active nanocarriers for ursolic acid with an IC50 below 20 µM, with an appropriate size for intravenous dosage (around 150 nm), high homogeneity of the sample (below 0.2), satisfactory encapsulation efficiency (up to 70%) and excellent stability. The new type of hybrid UA-PLGA nanoparticles represents a further step in the development of potentially effective PDAC therapies based on novel, biologically active, and promising triterpenoids.