Functional analysis of the placenta-specific enhancer of the human glycoprotein hormone alpha subunit gene. Emergence of a new element

Placental expression of the alpha subunit gene of the human glycoprotein hormones requires a multicomponent enhancer composed of tandem cAMP-response elements and an adjacent upstream regulatory element. Based on recent studies indicating that the upstream regulatory element includes binding sites for more than one protein, we investigated how functional activity correlated with these binding sites. Through extensive replacement mutagenesis of the native promoter regulatory region, we provide the first functional map of the upstream regulatory element. Within this region, we find that distinct proteins interact with three overlapping binding sites. While each site is functionally significant, no single site is essential or displays clear dominance. This is surprising since one of the sites binds a placenta-specific protein that heretofore has been regarded as essential for activity of the human alpha subunit placenta-specific enhancer. Consequently, our refined functional map of the upstream regulatory element reveals a complex combinatorial code that directs expression of the human alpha subunit gene to placenta.     

基于改进信号注入配电网单相接地故障定位研究

针对配电网单相接地故障定位难的问题,阐述了传统信号注入法的基本原理,提出了改进信号注入法,建立了matlab/simulink仿真模型。通过Matlab仿真实例验证,该方法有效可行,实现了在线故障定位,有效地缩短了停电时间和减少停电次数,提高了配电网供电质量稳定性。     

全桥拓朴隔离式开关电源主变压器磁设计

全桥拓朴结构的隔离式开关电源能在较小的尺寸下实现较高的功率变换,是高功率密度电源的重要电路结构,其高频主变压器磁心一般选择低损耗功率锰锌软磁铁氧体材料,其设计对电源的尺寸、性能有显著影响.磁设计的目的是用尽可能小的磁心传输尽可能高的功率,但受到两个限制性条件的制约,即磁心的发热和磁饱和.在散热充分的情况下,限制性条件就...     

混凝土坝裂缝变形的不确定性分析模型

混凝土坝裂缝的发生和发展受到不确定因素影响。将大坝系统及其安全监控看作一个受诸多因素影响的不确定性系统,结合裂缝实测资料,应用近似推理模型和信息分配法,建立了裂缝变形的不确定性分析模型。     

鄂尔多斯盆地高自然伽马异常特征

为了加深对盆地深部和浅部高自然伽马异常特征综合分析研究,揭示高自然伽马异常地质成因,从而 促进盆地多种能源矿产协同开发研究,针对收集大量的地球物理测井资料、地质资料及其他相关成果,绘制并分 析了相关成果图。研究中遵循从单孔测井曲线特征分析到典型剖面研究,并按层对高自然伽马的平面分布特征 进行分析研究。化验测试结果和自然伽马能谱测井成果研究表明,在本区,自然伽马异常增大主要是由于铀的 活化和聚集,导致铀元素增加而引起。相关研究表明,盆地深部和浅部的铀异常具有一定的关系,盆地历经的沉 积环境变迁是铀元素富集的主导因素。     

天然气加热炉的腐蚀原因及防护对策

天然气加热炉的腐蚀是一个普遍现象.本文从引起腐蚀的因素人手,详细剖析腐蚀发生的原因,提出监测和治理相结合的方法,系统地介绍了几种常用的防腐技术,包括添加缓蚀剂、使用耐腐蚀材料、界面涂层、电化学防腐及其它相关防腐技术.     

An automatic braking system that stabilizes leukocyte rolling by an increase in selectin bond number with shear

Wall shear stress in postcapillary venules varies widely within and between tissues and in response to inflammation and exercise. However, the speed at which leukocytes roll in vivo has been shown to be almost constant within a wide range of wall shear stress, i.e., force on the cell. Similarly, rolling velocities on purified selectins and their ligands in vitro tend to plateau. This may be important to enable rolling leukocytes to be exposed uniformly to activating stimuli on endothelium, independent of local hemodynamic conditions. Wall shear stress increases the rate of dissociation of individual selectin-ligand tether bonds exponentially (, ) thereby destabilizing rolling. We find that this is compensated by a shear-dependent increase in the number of bonds per rolling step. We also find an increase in the number of microvillous tethers to the substrate. This explains (a) the lack of firm adhesion through selectins at low shear stress or high ligand density, and (b) the stability of rolling on selectins to wide variation in wall shear stress and ligand density, in contrast to rolling on antibodies (). Furthermore, our data successfully predict the threshold wall shear stress below which rolling does not occur. This is a special case of the more general regulation by shear of the number of bonds, in which the number of bonds falls below one.     

低频波动条件下流体平面渗流模型研究

低频振动采油技术已成为一项重要的物理增产、增注措施,但其理论研究水平目前还相对滞后于现场应用.针对这一问题,运用渗流力学、波场传播的基本原理,建立了低频波动条件下地层流体的渗流模型,定量描述了地层、流体参数与振动采油压力波动之间的关系,并利用解析方法分析计算,认为低频波场主要受储层渗透率和振动频率两大因素影响.振动采油现场施工可以通过适当调整振动频率改善施工效果.     

Orally active, hydrolytically stable, semisynthetic, antimalarial trioxanes in the artemisinin family

In only three chemical operations, natural trioxane lactone artemisinin (1) was converted into a series of C-10 carbon-substituted 10-deoxoartemisinin compounds 4-9. The three steps involved lactone reduction, replacement of the anomeric lactol OH by F using diethylaminosulfur trifluoride, and finally boron trifluoride-promoted substitution of F by aryl, heteroaryl, and acetylide nucleophiles. All of these C-10 nonacetal, chemically robust, enantiomerically pure compounds 4-9 have high antimalarial potencies in vitro against Plasmodium falciparum malaria parasites, and furans 5a and 5b and pyrrole 7a are antimalarially potent also in vivo even when administered to rodents orally.     

Cation transport: an example of structural based selectivity

Through the high-resolution structure of the gramicidin A channel in lamellar phase lipids and the characterization of specific ion peptide interactions, fundamental principles for ion channel selectivity and conductance efficiency are illustrated with atomic resolution detail. Delocalized cation binding in the first turn of the helix reduces the unfavorable entropy contribution upon binding. Stepwise dehydration minimizes the energy barrier for cation entry and provides valence selectivity in this channel. Three or more water molecules in the monovalent cation binding site result in flexibility in the cation solvation environment causing weak cation size selectivity. Lack of cation induced structural modification avoids the formation of a significant energy barrier, thus permitting efficient cation transport.