Disruption of cellular translational control by a viral truncated eukaryotic translation initiation factor 2alpha kinase homolog

Phosphorylation of eukaryotic translation initiation factor 2alpha (eIF2alpha) is a common cellular mechanism to limit protein synthesis in stress conditions. Baculovirus PK2, which resembles the C-terminal half of a protein kinase domain, was found to inhibit both human and yeast eIF2alpha kinases. Insect cells infected with wild-type, but not pk2-deleted, baculovirus exhibited reduced eIF2alpha phosphorylation and increased translational activity. The negative regulatory effect of human protein kinase RNA-regulated (PKR), an eIF2alpha kinase, on virus production was counteracted by PK2, indicating that baculoviruses have evolved a unique strategy for disrupting a host stress response. PK2 was found in complex with PKR and blocked kinase autophosphorylation in vivo, suggesting a mechanism of kinase inhibition mediated by interaction between truncated and intact kinase domains.     

Fracture analysis of materials with periodic microstructure by the representative cell method

The periodic structure of some natural and especially man-made materials can be manifested not only on an atomic but also on a larger scale. Investigation of mechanical properties of these materials usually hinges on well-developed homogenization methods. On the other hand, these methods are not suitable for fracture analysis where the knowledge of the local stress-strain fields near a flaw (a crack) is required. The result is obtained by the use of the representative cell method based on the discrete Fourier transform. This method enables one to determine the exact stress distribution in a periodic structure subjected to arbitrary loading. Direct application of the method is impossible since the crack violates the translational symmetry defined by the material microstructure. This obstacle is overcome by application of the fictitious loading to the uncracked body at the line where the crack is to be located. The amplitude of the loading is adjusted in order to fulfill the boundary conditions imposed on the crack faces. The compatibility equation for deriving this amplitude is obtained by the use of the corresponding Green function, which is found in a closed form. Fracture problems for the two types of materials with a periodic microstructure are considered. The first one is a composite material consisting of dissimilar isotropic elastic layers arranged periodically. The second periodic microstructure is a 2D infinite beam lattice modeling a cellular material. The analysis of the failure process in the latter case shows that in contrast to the case of homogeneous material, the crack propagation path is not defined by the condition of zero Mode II stress intensity factor.     

Prophylactic Greenfield filters: acute complications and long-term follow-up

The efficacy of prophylactic vena caval filters (VCF) in reducing morbidity and mortality from pulmonary embolism (PE) in high-risk trauma patients has been shown, but minimal follow-up data is currently available. VCFs were prophylactically placed in 110 patients between August 1991 and June 1995. There was an early VCF complication rate of 7%. Twenty-two patients died; the remaining 88 patients formed the basis for the follow-up study. Forty-five patients were located and interviewed by phone, and 30 of these patients (34%) returned for evaluation. The mean follow-up time was 18 months (range, 4-42 months). There was no incidence of caval thrombosis on follow-up. Eleven patients had physical findings, and duplex evidence consistent with postphlebitic syndrome. An additional three patients had evidence of old deep venous thrombosis (DVT) by duplex, but no significant symptomatology. VCF are effective in preventing PE related deaths and have few major complications. The long-term morbidity associated with posttraumatic venous thrombosis is significant. This morbidity is related not to PE or VCF, but to the underlying DVT. Improved strategies against DVT are necessary.     

Connectivity Based k-Hop Clustering in Wireless Networks

In this paper we describe several new clustering algorithms for nodes in a mobile ad hoc network. The main contribution is to generalize the cluster definition and formation algorithm so that a cluster contains all nodes that are at distance at most k hops from the clusterhead. We also describe algorithms for modifying cluster structure in the presence of topological changes. We also proposed an unified framework for most existing and new clustering algorithm where a properly defined weight at each node is the only difference in otherwise the same algorithm. This paper studied node connectivity and node ID as two particular weights, for k=1 and k=2. Finally, we propose a framework for generating random unit graphs with obstacles.     

Some aspects of the biology of Dermestes maculatus Degeer (Coleoptera,Dermestidae) in dried fish

The development of Dermestes maculatus Deg. in dried fish has been studied under uncontrolled laboratory conditions. Females laid eggs within 12 hr of copulation, and oviposition was improved by the presence of free water. Hatching occurred about 48 hr after oviposition. The characteristic appearance of the egg and the changes that occurred during incubation and hatching are described. Larval development was completed in 33·5 days during which seven moults occurred and a body length of 14 mm was attained. Larval life was prolonged by crowding. When intact pieces of fish were available, the last instar larva bored into one of them and pupated within the hardened larval skin, but when ground fish was provided, a quiescent prepupal stage was observed. The adult emerged about 11 days after the last instar larval stage, irrespective of the mode of pupation.     

Conduction in thin polymer films

Thin polymer films have been made from a variety of starting monomers and their electrical conduction has been studied.The films are produced both by ultraviolet surface photolysis and by a cold-cathode glow-discharge technique. The samples are made in the form of a capacitor sandwich on a glass substrate with aluminium electrodes. The whole sample is made in a high-vacuum system, without breaking vacuum, using out-of-contact masking techniques.Generally, depending on the starting monomer, the polymer films are short-free down to a thickness of about 100 Å, and electric fields up to 10⁷ V/cm may be sustained.The current/voltage characteristics of the films have been investigated as a function of temperature and thickness. One theory to account for the conduction in these materials is presented and compared with other approaches to the problem.     

An analytical model for snapback in n-channel SOI MOSFET's

An analytical snapback model for n-channel silicon-on-insulator (SOI) transistors with body either tied to the source or floating is been presented. The snapback is modeled as a nonlinear feedback system leading to negative transconductances from which the jump in current can occur at the point of instability. The crux of this model is based on the strong dependence of the transistor threshold voltage on the body potential when the body potential is above the transistor surface potential at strong inversion. No parasitic bipolar action is invoked to account for the snapback phenomena. The model correctly predicts the occurrence of hysteresis/latch phenomena and the conditions under which the current jump occurs despite some gross approximations in the electric field and the injection level. Results obtained from this model show good agreement with experimental data measured from SIMOX devices fabricated on 0.3-μm epi film     

Learning and chronobiological regulation following experimental damage to the coronary-artery wall of albino rats]

Forty male albino rats were used to study the relationship between experimentally (concentrated hydrochloric acid) produced local structural changes of the coronary artery wall and central nervous information processing and chronobiological events, respectively. The alterations of the coronary arteries caused by this treatment did not produce infarction but initiated on the whole length of the arterial branch proliferative processes that are complete within 12 weeks following the intervention. Central nervous information processing and chronoregulatory processes showed, immediately after exposure to hydrochloric acid, pronounced malfunctions followed by a stage of hyperreactivity (4 weeks following the damage). At 8 weeks from the intervention, the parameters of information processing and chronobiological events of the CNS again equalled the control values. Since the central nervous functions were back to normal earlier than the structural alterations, the results are interpreted as reflecting CNS-controlled interactions between the complex of visceral afference and efference, the regulation of which is determined by the CNS after coronary damage in such a way as to restore the adaptational capacity of the organism by a predominance of sanogenetic processes.     

Fast multiplication in VLSI using wave pipelining techniques

Wave pipelining is a design methodology that can increase the clock frequency of digital systems. Also known asmaximum-rate pipelining, it has long been considered a technique for approaching the physical speed limit of a digital circuit. Unlike conventional pipelining, wave pipelining does not require internal clocked elements to increase throughput. The synchronization of internal computations is achieved by balancing inherent RC delays of combinational logic elements, thus allowing circuits to be pipelined at a very fine-grain level. In this article, we describe the design of a 16×16 wave-pipelined multiplier using a 1.0 μm CMOS process. The multiplier is designed using a conventional static CMOS technology. Simulation results show a speedup of about 7× over a nonpipeline implementation.     

Organic Phenolic Configurationally Locked Polyene Single Crystals for Electro‐optic and Terahertz Wave Applications

We investigate a configurationally locked polyene (CLP) crystal 2‐(3‐(4‐hydroxystyryl)‐5,5‐dimethylcyclohex‐2‐enylidene)malononitrile (OH1) containing a phenolic electron donor, which also acts as a hydrogen bond donor. The OH1 crystals with orthorhombic space group Pna2₁ (point group mm2) exhibit large second‐order nonlinear optical figures of merit, high thermal stability and very favorable crystal growth characteristics. Higher solubility in methanol and a larger temperature difference between the melting temperature and the decomposition temperature of OH1 compared to analogous CLP crystals, are of advantage for solution and melt crystal growth, respectively. Acentric bulk OH1 crystals of large sizes with side lengths of up to 1 cm with excellent optical quality have been successfully grown from methanol solution. The microscopic and macroscopic nonlinearities of the OH1 crystals are investigated theoretically and experimentally. The OH1 crystals exhibit a large macroscopic nonlinearity with four times larger powder second harmonic generation efficiency than that of analogous CLP crystals containing dimethylamino electron donor. A very high potential of OH1 crystals for broadband THz wave emitters in the full frequency range of 0.1–3 THz by optical rectification of 160 fs pulses has been demonstrated.