Low density lipoprotein catabolism is enhanced by the cleaved form of alpha-1-antitrypsin

The frequent occurrence of hypocholesterolaemia following inflammatory processes is well known but unexplained. Elevated plasma levels of serine proteinase inhibitors (serpins) and their complexes with target enzymes have been demonstrated in inflammatory, malignant and infectious diseases which are also often accompanied by low plasma cholesterol levels. Under inflammatory conditions, uncomplexed, but cleaved inactive serpins arising from slow deacylation of the serpin-proteinase complex may be present in the circulation. To determine the influence of native and cleaved forms of serpins, such as alpha-1-antitrypsin (AAT), on lipoprotein metabolism, we investigated the effect of these forms on low density lipoprotein (LDL) catabolism in human HepG2 cell line. We have found that the cleaved form of AAT in concentrations from 125 to 2000 nmol l-1 stimulates LDL binding to the HepG2 cells, by up to 49% with a subsequent increase in LDL uptake and degradation of up to 79 and 65% respectively. Native AAT was also found to increase LDL binding and internalization by 20-25%, independently of the amount of AAT added, an effect most probably due to the cleaved form of AAT produced by local proteolysis of native AAT incubated in the cell culture. Moreover we have shown that the cleaved form of AAT interacts with LDL in vitro, and that such an interaction abolishes AAT ability to stimulate LDL binding and internalization. This study for the first time describes the ability of the cleaved form of AAT to stimulate LDL binding and internalization in HepG2 cell culture, and provides evidence that hypocholesterolaemia occurring during inflammatory processes may be mediated by cleaved forms of serpins.     

视频编码中的块运动估计算法

在视频压缩的一些国际标准，如Ｈ．２６１，Ｈ．２６３，ＭＰＥＧ０－１，ＭＰＥＧ－２，ＨＤＴＶ中，视频系统编码器的复杂性最主要取决于运动估计。下文以ＭＰＥＧ－２编码器为例，通过计算机模拟实验，得出了一些常用运动估计算法的对比实验结果，以及采用常用的几种匹配函数的对比实验结果。     

分离纯化低聚异麦芽糖的酵母菌菌株筛选

对山东寿光市保护地蔬菜施肥状况的调查及产生的问题综合评价表明 :寿光市保护地蔬菜施肥用量很大 ,但极不平衡 ,化学氮肥及磷肥施用量过大造成了土壤的盐化、酸化、中微量元素的缺乏及环境的污染 ,使得蔬菜的产品品质下降 ,蔬菜NO-3 含量较高 ,产投比降低。钾肥用量太少 ,植物抗逆性差 ,病虫害严重。提出了蔬菜合理施肥的几项建议。     

80年代黄河中游来沙减少的原因分析

80年代黄河中游来沙量减少,主要原因是降雨量减少和降雨强度减小,流域综合治理也起到了减沙作用。据分析,降雨因素减沙约占总减沙量的70％,流域综合治理减沙约占30％。     

一机多控视频点播系统

介绍一种自行设计、开发的一机多控视频点播系统，讲述了系统组成及设计方案，它具有服务功能多，操作方便、性能价格比高等特点。     

Achieving real-time object detection and tracking under extreme conditions

In this survey, we present a brief analysis of single camera object detection and tracking methods. We also give a comparison of their computational complexities. These methods are designed to accurately perform under difficult conditions such as erratic motion, drastic illumination change, and noise contamination.     

应用硫酸铵提高乌拉嗄金矿石浮选回收率试验研究

经试验发现应用一种化学肥料硫酸铵可以显著提高乌拉嘎金矿石的浮选回收率。闭路试验尾矿工艺矿物学鉴定证明，以硫酸铵作为调整剂的浮选过程中，微细粒单体金和金与石英边生体被有效回收。     

康山金矿地质地球化学特征及其成因

康山金矿产在豫西熊耳山古隆起区的结晶基底太华群和盖层中元古界熊耳群地层接触界面附近，矿体严格受构造破碎蚀变带控制。流体包裹体，稳定同位素等研究表明，成矿物质来自太华群变质岩，成矿流体的性质与变质热液截然不同，而与花岗岩浆热液相近似。该矿床的形成与燕山期发生的同构造－花岗岩浆期热液活动有关，金沉淀成矿阶段有大气水的混入。     

黑土土壤质量演变初探I：不同开垦年限黑土主要质量指标演变规律

根据土壤质量的定义和研究方法 ,对黑土的主要质量指标演变规律进行初步研究。结果表明 :黑土的颗粒组成受开垦历史影响的程度较小 ,其质地没有发生显著变化 ;黑土的有机质、全氮、全硫和全磷随着黑土开垦时间的增加而不断降低 ;速效磷随黑土开垦时间的增加有增高的趋势。经过统计分析 ,黑土中粉砂(0.05～0.002mm)含量与细砂(0.25～0.05mm)和粘粒(<0.002mm)含量具有相关性,黑土有机质含量与全氮、全硫、全磷和速效钾具有明显的正相关。因此 ,初步认为黑土中粉粒和有机质含量可以作为土壤物理和养分方面的质量指标     

缝洞型碳酸盐岩油藏流动单元概念和研究方法探讨

流动单元对于提高油田采收率具有很大的实际意义,该项研究在砂岩油藏研究中已取得较大进展。对于缝洞型碳酸盐岩油藏,由于岩石结构构造、成岩后生作用、构造断裂作用、溶蚀作用等多因素对储集空间的影响,已有的流动单元概念及研究技术方法难于应用。以塔河油田为例,针对缝洞型碳酸盐岩地质特征,论证了碳酸盐岩储层渗流屏障的存在及其类型,探索性提出区别于砂岩油藏的缝洞型碳酸盐岩储层流动单元的概念。提出的研究思路和方法包括;油藏压力趋势分析法、井间生产干扰分析法、流体性质差异分析法、井间干扰试井法。结合储层发育特征,初步建立了缝洞型碳酸盐岩流动单元的划分标准。