Recently worldwide food safety authorities indicated the rise of foodborne outbreaks linked to Salmonella: this highlighted the need to intensify monitoring and apply more targeted controls to help manage the spread of the disease. The aim of this study was to assess the prevalence and distribution of Salmonella serotypes in 7 slaughterhouses, located in different areas of Naples province (Regione Campania, Italy). Meat samples collected from the slaughterhouses were submitted for standardized microbiological analysis in 2015. Results of routine testing for Salmonella spp. were analyzed and then compared to biochemical and molecular evaluations. Salmonella spp. were detected in 12% of 320 samples examined (39/320) and the isolation rates ranged from 87% (32 samples) for raw poultry meat to 13% (7 samples) for pork meat. Biochemical serotyping showed that approximately 50% of the isolates belonged to Salmonella enterica serotype Choleraesuis. Rapid detection methods, such as molecular analysis (polymerase chain reaction and gel electrophoresis), able to confirm food matrices contamination, represent a valid support to the fast identification of Salmonella species. A further aspect of the study consisted, indeed, on analyzing isolated strains through molecular evaluations. By amplifying bacterial DNA—using invA primers, selective for Salmonella—it was possible, in less than 3 h, to classify the isolates as Salmonella spp., confirming the results of microbiological outcomes. Results of distribution analysis, supported by rapid molecular approaches, showed the difficulty of reducing Salmonella risk on food chain. This emphasized the importance of periodic surveillance to prevent outbreaks. 相似文献
Migration of nanomaterials from food containers into food is a matter of concern because of the potential risk for exposed consumers. The aims of this study were to evaluate silver migration from a commercially available food packaging containing silver nanoparticles into a real food matrix (chicken meat) under plausible domestic storage conditions and to test the contribution of such packaging to limit food spoilage bacteria proliferation. Chemical analysis revealed the absence of silver in chicken meatballs under the experimental conditions in compliance with current European Union legislation, which establishes a maximum level of 0.010 mg kg–1 for the migration of non-authorised substances through a functional barrier (Commission Regulation (EU) No. 10/2011). On the other hand, microbiological tests (total microbial count, Pseudomonas spp. and Enterobacteriaceae) showed no relevant difference in the tested bacteria levels between meatballs stored in silver-nanoparticle plastic bags or control bags. This study shows the importance of testing food packaging not only to verify potential silver migration as an indicator of potential nanoparticle migration, but also to evaluate the benefits in terms of food preservation so as to avoid unjustified usage of silver nanoparticles and possible negative impacts on the environment. 相似文献
Metal shear panels represent a highly performing anti seismic system for medium–high rise buildings located in strong earthquake prone zones. A new configuration of dissipative shear panel is presented in the current paper. The base shear plate is made of a low-strength material, namely the heath treated EN-AW-1050A aluminium alloy, which has been already used by the authors for other types of dissipative shear panels. In this proposed new system, the main buckling modes are inhibited by the application of external devices placed in parallel to the base plate and not directly loaded by the external forces. Two shear panel samples are tested as prototypes, which are based on two different plate buckling inhibition strategies. In the former the out-of-plane displacements of only some parts of the base plate are restrained, whereas in the latter the restraining action of the external device is extended to the whole base plate. Experimental tests are presented in order to prove the performance of the proposed devices in terms of dissipative capacity and stiffness. In addition, the obtained results are compared with the ones related to other more conventional shear panel typologies, characterized by the same geometry and material, but in which buckling phenomena were mitigated by means of welded stiffeners, according to a more traditional practice. 相似文献
Liquid feeds are injected into fluidized bed reactors such as fluid cokers, fluid catalytic crackers and gas-phase polymerization reactors. In these industrial processes, it is of crucial importance to optimize the contact between the injected liquid and the bed solids to minimize agglomeration, to ensure good reactor operability, and to allow reactions to proceed under minimum heat and mass transfer limitations in order to maximize the yields of valuable products. It has been shown [P. House, M. Saberian, C. Briens, F. Berruti, E. Chan, Injection of a liquid spray into a fluidized bed: particle-liquid mixing and impact on fluid coker yields. Industrial & Engineering Chemistry Research 43 (2004) 5663-5669., S. Bruhns, J. Werther, An investigation of the mechanism of liquid injection into fluidized beds. AIChE Journal 51 (2005) 766-775] that the nozzle technology and the operating conditions have a significant effect on the quality of the liquid-solid interaction resulting from the injection of gas-atomized liquid feed. The goal of this study was to develop a rapid and reliable experimental technique to assess the liquid-solid contact efficiency resulting from the injection of a liquid feed into a fluidized bed. Air-fluidized silica sand particles were first charged by triboelectrification as a result of their random collisions with the inner walls of the fluidized bed. Immediately after the injection of water through an aerated nozzle, the fluidization air was stopped and the wetted bed solids were allowed to settle. While the bed was defluidized, the triboelectric charges accumulated on the particles migrated to a grounded electrode through the low-resistance paths offered by the conductive liquid. A stronger electric current flowing through the electrode indicated that the liquid was more evenly distributed on the solid particles. The intensity of the current flowing through the electrode was, therefore, used to define a spray nozzle performance index. This technique was used to examine the effect of increasing the nozzle aeration, and, specifically, the gas-to-liquid mass ratio (G/L) through the nozzle on the liquid-solid contact efficiency. The results showed that changing the nozzle geometry can change how the contact efficiency between atomized liquid and fluidized solids varies with (G/L), especially at relatively high G/L ratios. A model of the time-evolution of the electric current generated during defluidization of the bed solids is also presented. 相似文献
An efficient copper catalyst for the one-pot one-step hydrogenation?+?esterification of unsaturated free fatty acids is described. The high selectivity in hydrogenation promoted by copper, combined with the high activity in esterification observed with solid mixed oxides allows one to directly obtain stabilized methyl esters. 相似文献
Surface properties of epoxy coatings are modified by PDMS additives in cationic UV curing of a cycloaliphatic epoxy resin. The cured films show a very high hydrophobicity that does not depend on PDMS concentration, indicating that a threshold is reached even at 0.3 wt% additive. A slight increase of the water contact angle as a function of PDMS molecular weight is observed. The additive selectively modified the air‐side of the film, while the glass‐side retains the surface properties of the pure resin. This segregation phenomenon permits to obtain highly hydrophobic films with still good adhesion properties on polar substrates, which is an important advantage over common surface‐modified resins.
The aim of the study was to establish whether the visual cleanliness of cattle slaughtered was correlated to hide and carcass contamination as indicated by aerobic colony count (ACC), Enterobacteriaceae count (EC) and Escherichia coli count (ECC). Cattle in a slaughterhouse were visually inspected and assigned to a category from 1 (very clean) to 5 (very dirty) based on cleanliness. Fifteen animals for each category were randomly selected, hide and carcass sampled and analyzed for ACC, EC and ECC. Results showed that increasing dirt on cattle was associated with higher ACC, EC and ECC on hide and carcasses. Carcass ACC and ECC belonging to animals classified in cleanliness categories 3, 4 or 5 have a higher probability of exceeding the limits set by the Reg. EU 2073/2005. The study supports the conclusion that the pre-slaughter visual evaluation of animal cleanliness and application of corrective actions can be an effective aid to reduce carcass contamination. 相似文献