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81.
Kaluhannadige R. E. Padmathilake Wannakuwattewaduge Gerard Dilantha Fernando 《International journal of molecular sciences》2022,23(7)
Leptosphaeria maculans causes blackleg disease, which is one of the most destructive diseases of canola (Brassica napus L.). Due to the erosion of the current resistance in B. napus, it is pivotal to introduce new resistant genotypes to the growers. This study evaluated the potential of Rlm7 gene as resistance to its corresponding avirulence AvrLm7 gene is abundant. The Rlm7 line was inoculated with L. maculans isolate with AvrLm7; UMAvr7; and the CRISPR/Cas9 knockout AvrLm7 mutant, umavr7, of the same isolate to cause incompatible and compatible interactions, respectively. Dual RNA-seq showed differential gene expressions in both interactions. High expressions of virulence-related pathogen genes-CAZymes, merops, and effector proteins after 7-dpi in compatible interactions but not in incompatible interaction—confirmed that the pathogen was actively virulent only in compatible interactions. Salicyclic and jasmonic acid biosynthesis and signaling-related genes, defense-related PR1 gene (GSBRNA2T00150001001), and GSBRNA2T00068522001 in the NLR gene family were upregulated starting as early as 1- and 3-dpi in the incompatible interaction and the high upregulation of those genes after 7-dpi in compatible interactions confirmed the early recognition of the pathogen by the host and control it by early activation of host defense mechanisms in the incompatible interaction. 相似文献
82.
Anna Vallverdú-Queralt José Fernando Rinaldi de Alvarenga Ramon Estruch Rosa M. Lamuela-Raventos 《Food chemistry》2013
Sofrito is a key component of the Mediterranean diet, a diet that is strongly associated with a reduced risk of cardiovascular events. In this study, different Mediterranean sofritos were analysed for their content of polyphenols and carotenoids after a suitable work-up extraction procedure using liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (LC/ESI-LTQ-Orbitrap-MS) and liquid chromatography/electrospray ionisation tandem triple quadrupole mass spectrometry (LC/ESI-MS-MS). In this way, 40 polyphenols (simple phenolic and hydroxycinnamoylquinic acids, and flavone, flavonol and dihydrochalcone derivatives) were identified with very good mass accuracy (<2 mDa), and confirmed by accurate mass measurements in MS and MS2 modes. The high-resolution MS analyses revealed the presence of polyphenols never previously reported in Mediterranean sofrito. The quantification levels of phenolic and carotenoid compounds led to the distinction of features among different Mediterranean sofritos according to the type of vegetables (garlic and onions) or olive oil added for their production. 相似文献
83.
Drug-induced liver injury (DILI) is one of the leading causes of acute liver injury. Many factors may contribute to the susceptibility of patients to this condition, making DILI a global medical problem that has an impact on public health and the pharmaceutical industry. The use of mesenchymal stem cells (MSCs) has been at the forefront of regenerative medicine therapies for many years, including MSCs for the treatment of liver diseases. However, there is currently a huge gap between these experimental approaches and their application in clinical practice. In this concise review, we focus on the pathophysiology of DILI and highlight new experimental approaches conceived to improve cell-based therapy by the in vitro preconditioning of MSCs and/or the use of cell-free products as treatment for this liver condition. Finally, we discuss the advantages of new approaches, but also the current challenges that must be addressed in order to develop safer and more effective procedures that will allow cell-based therapies to reach clinical practice, enhancing the quality of life and prolonging the survival time of patients with DILI. 相似文献
84.
85.
Pooyan Ayar Fernando Moreno-Navarro Miguel Sol-Sánchez Mª Carmen Rubio-Gámez 《Materials and Structures》2018,51(1):25
Recovery capability of bituminous materials plays a significant role in the development of new technologies for extending the service life of asphalt pavements. This capability originates from various phenomena such as thixotropy, cooling, relaxation of hardening, or healing. However, their real effect on mechanical response is not clear. This article aims to investigate how rest periods (RPs) available between traffic loads can contribute to the damage recovery of bituminous materials. For this purpose, different types and durations of RPs were applied during the laboratory evaluation of fatigue resistance of these materials using the University of Granada Fatigue Asphalt Cracking Test method. The results indicate that the addition of RPs to the loading regime could lead to an extension in the fatigue life of bituminous materials. Additionally, an increase in the RP duration showed a positive impact on the resistance of the materials against cyclic loading. Nonetheless, these benefits are not only related to the recovery of lost properties during RPs, but also a growth in the amount of plastic deformations as a result of the applying RPs could delay the appearance of damages (i.e. cracking). Consequently, the bituminous material can tolerate a higher number of load cycles during fatigue test. 相似文献
86.
Fabíolo Moraes Amaral Luís Fernando C. Alberto Josaphat R. R. Gouveia Jr. 《Dynamical Systems: An International Journal》2018,33(1):113-135
A complete characterization of the stability boundary of an asymptotically stable equilibrium point in the presence of type-k saddle-node non-hyperbolic equilibrium points, with k ≥ 0, on the stability boundary is developed in this paper. Under the transversality condition, it is shown that the stability boundary is composed of the stable manifolds of the hyperbolic equilibrium points on the stability boundary, the stable manifolds of type-0 saddle-node equilibrium points on the stability boundary and the stable centre and centre manifolds of the type-r saddle-node equilibrium points with r ≥ 1 on the stability boundary. This characterization is the first step to understanding the behaviour of stability regions and stability boundaries in the occurrence of saddle-node bifurcations on the stability boundary. 相似文献
87.
Energy-saving scheduling on IaaS HPC cloud environments based on a multi-objective genetic algorithm
Vila Sergi Guirado Fernando Lerida Josep L. Cores Fernando 《The Journal of supercomputing》2019,75(3):1483-1495
The Journal of Supercomputing - Nowadays, cloud computing is a growing scenario applied to many scientific and manufacturing areas due to its flexibility for adapting to highly demanding computing... 相似文献
88.
The purpose of this paper is to present an extension of the generalised supertwisting algorithm (STA) to the multivariable framework. We begin by introducing an algorithm that may be deemed as a linear, quasicontinuous, or discontinuous multivariable system, depending on the functions that define them. For the class represented by such an algorithm we prove the robust, Lyapunov stability of the origin and characterise the perturbations that preserve its stability. In particular, when its vector field is discontinuous or quasicontinuous our algorithm is endowed with finite‐time stability. Due to its resemblance to the scalar case, we denote such finite‐time stable systems as generalised multivariable STA. Furthermore, the class of finite‐time stable systems comprise the currently available versions of STAs. To finalise, by means of simulation examples, we show that our proposed finite‐time stable algorithms are well suited for signals online differentiation and highlight their dynamical traits. 相似文献
89.
The objective of this article is to provide a generalized framework of a novel method that investigates the problem of combining and fusing different types of measurements for pose estimation. The proposed method allows to jointly minimize the different metric errors as a single measurement vector in n-dimensions without requiring a scaling factor to tune their importance. This paper is an extended version of previous works that introduced the Point-to-hyperplane Iterative Closest Point (ICP) approach. In this approach, an increased convergence domain and a faster alignment were demonstrated by considering a four-dimensional measurement vector (3D Euclidean points + Intensity). The method has the advantages of the classic point-to-plane ICP method, but extends this to higher dimensions. For demonstration purposes, this paper will focus on a RGB-D sensor that provides colour and depth measurements simultaneously and an optimal error in higher dimensions will be minimized from this. Results on both, simulated and real environments will be provided and the performance of the proposed method will be carried on real-time visual SLAM. 相似文献