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991.
A novel, microwave-assisted, high-temperature UV digestion procedure was developed for the accelerated decomposition of interfering dissolved organic carbon (DOC) prior to trace element analysis of liquid samples such as, industrial/municipal wastewater, groundwater, and surface water, body fluids, infusions, beverages, and sewage. The technique is based on a closed, pressurized, microwave digestion device. UV irradiation is generated by immersed electrodeless Cd discharge lamps (228 nm) operated by the microwave field in the oven cavity. To enhance excitation efficiency an antenna was fixed on top of the microwave lamp. The established immersion system enables maximum reaction temperatures up to 250-280 degrees C, resulting in a tremendous increase of mineralization efficiency. Compared to open UV digestion devices, decomposition time is reduced by a factor of 5 and the maximum initial concentration of DOC can be raised by at least a factor of 50. The system's performance on a real-type sample was evaluated for the mineralization of skimmed milk (IRMM, CRM 151) and subsequent determination of trace elements using standard spectroscopic techniques. Recovery for Cd (109%), Cu (112%), Fe (99%), and Pb (96%) showed good agreement with the 95% confidence interval of the certified values. 相似文献
992.
Loudwig S Nicolet Y Masson P Fontecilla-Camps JC Bon S Nachon F Goeldner M 《Chembiochem : a European journal of chemical biology》2003,4(8):762-767
The photoregulation of the catalytic activity of butyrylcholinesterase (BChE) was investigated by treating the enzyme with a newly developed carbamylating reagent, N-methyl-N-(2-nitrophenyl)carbamoyl chloride, which has proved to be an efficient photoremovable alcohol-protecting group. BChE was efficiently inhibited in a time- and concentration-dependent manner, and the enzyme could be protected against inhibition by active-site-specific ligands (that is, tacrine). The inactivation kinetics showed a biphasic character, which can be analyzed as the result of the existence of two conformational states in solution. Pseudo-irreversible inactivation of BChE, which results from catalytic serine carbamylation, was suggested by recovery of the enzyme activity after dilution and was demonstrated by X-ray crystallography. Remarkably, the 3D structure of the carbamylated BChE conjugate showed a nonambiguous carbamylation of the catalytic serine residue as the only chemical modification on the protein. The photoreversibility of the enzyme inactivation was analyzed by irradiating the inactivated enzyme at 365 nm and was shown to reach completion in some conditions. The efficient and specific "caging" of BChE, together with the availability of carbamylated BChE crystals, will offer a unique possibility to study the catalytic properties of this enzyme by kinetic crystallography after cryophotolytic uncaging of the enzyme conjugate crystals. 相似文献
993.
Hirschberger Gilad; Florian Victor; Mikulincer Mario 《Canadian Metallurgical Quarterly》2005,50(3):246
Objective: To examine whether death anxiety underlies emotional reactions toward people with disabilities (PWDs). Study Design: In Study 1, participants were primed with thoughts of death, read a vignette describing a PWD, and completed a compassion scale. Study 2 replicated the design of Study 1 and also examined the impact of target ethnicity. Studies 3 and 4 assessed the impact of physical disability on death-related cognitions and on fear of personal death. Results: Men reacted to death primes by emotionally withdrawing from a PWD and reporting less compassion, whereas women exhibited more compassionate responses. These differences in compassion were reflected in the death-related cognitions and fears sparked by physical disability. Conclusions: The human need to manage the terror of death shapes emotional reactions toward PWDs. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
994.
Large Grid systems not only provide massive aggregated computing power but also an unprecedented amount of distributed storage space. Unfortunately, the dynamic behavior of the Grid, caused by varying resource availability, unpredictable data updates, and the impact of local site policies makes it difficult to exploit the full capabilities of Data Grids.We present an analytical model for determining the optimal number of replica servers, catalog servers, and catalog sizes to guarantee a given overall reliability in the face of unreliable components. Our model captures the characteristics of peer-to-peer-like environments as well as that of Grid systems. Empirical simulations confirm the accuracy of our analytical model. 相似文献
995.
996.
Dieter Schmalstieg Gerhard Schall Daniel Wagner István Barakonyi Gerhard Reitmayr Joseph Newman Florian Ledermann 《Computer Graphics and Applications, IEEE》2007,27(4):48-57
Mobile augmented reality requires georeferenced data to present world-registered overlays. To cover a wide area and all artifacts and activities, a database containing this information must be created, stored, maintained, delivered, and finally used by the client application. We present a data model and a family of techniques to address these needs. 相似文献
997.
Florian ‘Floyd’ Mueller Gunnar Stevens Alex Thorogood Shannon O’Brien Volker Wulf 《Personal and Ubiquitous Computing》2007,11(8):633-645
Sport is a domain full of movement-based interactions. These interactions typically have positive health effects as well as
an impact on social bonding. We have investigated ways in which computer augmented devices can lead to new sport experiences
and explored opportunities to combine physical activities with remote social bonding. Three prototypes have been implemented
which showcase movement-based interaction in sports. “Breakout for Two” allows geographically distant users to play a physically
exhausting ball game together. “FlyGuy” gives users a hang-glide experience controllable through body movement. “Push’N’Pull”
uses isometric exercise equipment over a network to encourage users to complete a cooperative game whilst performing intense
muscular actions. A comparison of these applications shows that such movement-based interaction in a networked environment
allows players in different locations to achieve a work out and also to socialize. Based on these projects, we conclude with
practical design implications for future Exertion Interfaces. 相似文献
998.
We consider the application of revenue management to a make-to-order manufacturing company with limited inventory capacity. Orders with different profit margins arrive stochastically over an infinite time horizon and the company has to decide which orders to accept and which orders to reject. We model the problem with a discrete-time Markov decision process and propose a heuristic procedure. In numerical tests we show the potential benefit of using revenue management instead of a FCFS policy and assess the performance of the heuristic procedure.We thank two anonymous referees for their valuable suggestions that helped to improve the quality of the paper. 相似文献
999.
A multifunctional hybrid glass fiber-reinforced polymer (GFRP)/steel joint has been developed for the transfer of compression and shear forces in thermal insulation sections of concrete slab structures used in building construction. The new pultruded cellular GFRP element improves considerably the energy savings of buildings due to its low thermal conductivity. The quasi-static behavior of the GFRP element in insulating and load-transferring joints at the fixed support of cantilever beams was investigated. Two loading modes were investigated: a moment dominant mode and a shear dominant mode. Results show that the GFRP element is not critical at the ultimate limit state. Ductile failure occurs either in the concrete during yielding of the steel bars, or only in the steel bars that penetrate the hybrid GFRP/steel joint. In moment mode, the GFRP element only transfers the compressive forces from the bending moments. In shear mode, in addition to the moment transfer, about 43–63% of the shear forces are transferred in the element webs at ultimate limit state due to tilting of the element. The application proves that multifunctionality can lead to competitive solutions for GFRP composites used in load-carrying components and can compensate for the relatively high material cost. 相似文献
1000.
Hiss JA Bredenbeck A Losch FO Wrede P Walden P Schneider G 《Protein engineering, design & selection : PEDS》2007,20(3):99-108
Identification of molecular features that determine peptide interaction with major histocompatibility complex I (MHC I) is essential for vaccine development. We have developed a concept for peptide design by combining an agent-based artificial ant system with artificial neural networks. A jury of feedforward networks classifies octapeptides that are recognized by mouse MHC I protein H-2K(b). Prediction accuracy yielded a correlation coefficient of 0.94. Peptides were designed in machina by the artificial ant system and tested in vitro for their MHC I stabilizing effect. The behavior of the search agents during the design process was controlled by the jury network. The experimentally determined prediction accuracy was 89% for the designed stabilizing and 95% for the non-stabilizing peptides. Novel H-2K(b) stabilizing peptides were conceived that reveal extensions of known residue motifs. The combined network-agent system recognized context dependencies of residue positions. A diverse set of novel sequences exhibiting substantial activity was generated. 相似文献