Growth promotion of Bifidobacterium species by poultry bone and meat trimming hydrolyzate

Abstract: The growth of bifidobacteria that are employed in the production of functional food is often slow or limited, even on synthetic media. In this study, we investigated whether a peptide hydrolyzate (functional animal protein [FAP]), from poultry bones and meat trimmings, could be a potential source of growth stimulators. The bifidogenic activity of FAP on 18 strains of Bifidobacterium species was assessed via 2 different techniques: turbidimetric measurements and a direct count by fluorescence microscopy. Growth experiments were performed in B12 broth as the basal medium, B12 broth supplemented with N‐acetylglucosamine, and B12 broth supplemented with FAP. FAP supplementation yielded the highest maximum optical density (OD) and count values. The use of the microscopic fluorescence counts allowed for better evaluation of the extent of growth and assessment of the viability of cells. FAP from poultry bones and meat trimmings has potential as a growth stimulator for different bifidobacteria of human origin. FAP is a promising ingredient for inclusion in industrial media that are used to culture probiotic strains, including bifidobacteria, because it supports growth very well and maintains cells at a high level of viability. Practical Application: Proteinaceous hydrolyzate can be considered a promising ingredient for industrial media that are used to culture probiotic strains, including bifidobacteria, because it improves bacterial growth and maintains cells at a high level of viability.     

Flavour formation in pork semimembranosus: Combination of pan-temperature and raw meat quality

Flavour development and overall eating quality of pork semimembranosus were investigated with regard to different raw meat qualities (feeding/fasting strategy; control/low glycogen level, gender; castrate/female, slaughter live-weight; 84 kg/110 kg) combined with frying temperature (150 °C/240 °C). It was further investigated whether the precursor levels of glycogen, IMP, inosine, and hypoxanthine in the raw meat were correlated to the raw meat quality and fried/grilled attributes. Pork schnitzels were fried on a pan (155 °C) or grill–pan (240–250 °C) to a core temperature of 70 °C. Frying temperature was the one factor with greatest influence on the sensory attributes, and pan-grilled schnitzels had significantly higher scores in fried/grilled attributes regardless of meat quality compared to pan-fried schnitzels. Texture was not appreciably influenced by any treatment. There was no correlation between precursor levels and raw meat qualities or fried sensory attributes. Gender and slaughter live-weight had no pronounced influence on flavour and overall eating quality.     

Database Repairs and Analytic Tableaux

In this article, we characterize in terms of analytic tableaux the repairs of inconsistent relational databases, that is databases that do not satisfy a given set of integrity constraints. For this purpose we provide closing and opening criteria for branches in tableaux that are built for database instances and their integrity constraints. We use the tableaux based characterization as a basis for consistent query answering, that is for retrieving from the database answers to queries that are consistent with respect to the integrity constraints.     

A mass balance model describing multiyear fate of organochlorine compounds in a high Arctic lake

Data collected over a 3-year study of a high arctic watershed and lake are used to understand the fate of organochlorine compounds (OCs) and form the basis of a mass balance contaminant fate model. The model uses the fugacity/aquivalence approach to describe OC dynamics between air, stream inflows and outflow, the water column, and surficial sediments. The steady-state model results indicate that stream inflows contributed from 96 to >99% of total chemical loadings, but 57-98% of total loadings were lost from the lake via the outlet, the percentage of which is controlled by the hydrologic regime of the high arctic lake. Conversely, only 0.4-3.4% of loadings were retained within the sediments due to the high export rate, minimal scavenging from the water column and low organic carbon fraction of the sediments. Using the unsteady-state model, which includes year-round processes, degradation was estimated to account for losses of 7-32% for the more persistent OCs and 42-50% for the less persistent OCs (alpha-HCH, gamma-HCH, and endosulfan I). If loadings were eliminated, water column concentrations would decline with half-lives <1 year for less persistent OCs and 1-2 years for the more persistent OCs, whereas the half-lives for OCs in sediment are 8-25 years.     

Waste from brewing (trub) as a source of protein for the food industry

The use of agro-industrial waste for food enrichment can be limited, due to taste, odour, colour and other unpleasant characteristics. Trub (brewing waste) has important nutrients, such as proteins and carbohydrates, and phytochemical compounds; however, its applications are hindered by the astringent flavour. In order to reduce the bitterness of trub, aqueous extractions were realized at high temperature (100 °C per 1 h) in five steps. Two fractions were obtained; a liquid fraction (containing bitter compounds) and a solid fraction (the trub after the extraction process; TAP). Both fractions and the trub before the extraction process (TBP) were evaluated. The bitterness of TBP was significantly reduced in TAP while the protein content increased; TAP presented a branched network and changes in its structure. The reduction in bitterness and the high protein content allows the use of trub in the food industry.     

Atmospheric distribution and long-range transport behavior of organochlorine pesticides in North America

Annually averaged concentrations and enantiomeric compositions of organochlorine pesticides (OCPs) in air were determined in 2000/2001 at 40 stations across North America using XAD-based passive samplers. Absolute concentration differences acrossthe continent, the skewness and kurtosis of the concentration distribution, the relative abundance of parent compound and metabolites, and the chiral signatures can identify regional sources of OCP to the atmosphere. Specifically, air samples collected in the southeastern United States had elevated concentrations of chlordane-related compounds, higher ratios of trans- to cis-chlordane and heptachlor to heptachlor epoxide, as well as higher enantiomeric fractions of trans-chlordane as compared to other regions, suggesting continued release of microbially unprocessed chlordane to the regional atmosphere. Similarly, greatly elevated concentrations of p,p'-DDT, low relative abundance of the metabolite DDE, and a racemic composition of o,p'-DDT in samples from southern Mexico and Belize indicate recent use of DDT in these regions. Belize is also a potential source region for dieldrin. Reflecting its continued use in North America, endosulfan is now one of the most abundant and ubiquitous OCPs in the continental atmosphere. In contrast to these OCPs, air concentrations of penta- and hexachlorobenzene vary only by factors of 2-4 across the continent, reflecting a long atmospheric residence time and few primary sources. Atmospheric levels of the chlorobenzenes, alpha-endosulfan, and p,p'-DDE increase with elevation in the Canadian Rocky Mountains. Empirical travel distances for the OCPs derived from latitudinal concentration profiles are in good agreementwith model-derived indicators of long-range transport potential and, in particular, lead to a similar categorization of the OCPs. Large-scale passive air sampling networks are suitable for monitoring compliance with, and effectiveness of, regulatory control measures and for establishing experimentally the atmospheric long-range transport behavior of organic air pollutants.     

The influence of spatial ability on gender differences in mathematics college entrance test scores across diverse samples.

The relationship between mental rotation ability and gender differences in Scholastic Aptitude Test-Math (SAT-M) across diverse samples was investigated. Talented preadolescents, college students and high- and low-ability college-bound youths, totaling 760, were administered the Vandenberg Mental Rotation Test. Gender comparisons showed male outperforming female students in both mental rotation and SAT-M for all 3 high-ability groups but not for the low-ability group. For all female samples, mental rotation predicted math aptitude even when SAT-Verbal was entered first into the regression. For male samples, the relationship varied as a function of sample. When mental rotation ability was statistically adjusted for, the significant gender difference in SAT-M was eliminated for the college sample and the high-ability college-bound students. This suggests that spatial ability may be responsible in part for mediating gender differences in math aptitude among these groups. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Naive optics: Understanding the geometry of mirror reflections.

Paper-and-pencil tasks showed that many university students believed that when laterally approaching a mirror, they would see a reflection in the mirror before it was geometrically possible. Participants failed to adequately factor in the observer's location in the room. However, when asked about the behavior of a ray of light, participants knew about the law of reflection. No differences between psychology and physics students were detected, suggesting that the phenomenon is widespread and refractory to training. The findings were replicated with observers making judgments about image locations in a real room using a pretend mirror. Possible heuristics about mirror reflection that might explain the data are discussed. Naive optics is a promising venue to further knowledge of how observers understand basic laws of physics. (PsycINFO Database Record (c) 2010 APA, all rights reserved)