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991.
The deployment of electric taxi fleets is highly desirable from a sustainable point of view. Nevertheless, the weak autonomy of this kind of taxis requires a careful operation. The management of such a fleet looking to prevent possible run out of power is studied in this paper. The related question of locating charging terminals for the taxis is also addressed. Methods for dealing with these tasks are proposed and their efficiency is proved through simulations. 相似文献
992.
Krishnaswamy Hariharan Ngoc-Trung Nguyen Nirupam Chakraborti Frédéric Barlat 《Materials and Manufacturing Processes》2015,30(4):403-413
The texture induced anisotropy of yield strength in cold rolled sheet metals is modeled using anisotropic yield criteria. The classical and other optimization methods used so far to determine the yield coefficients are limited by fixed set of experimental data, initial guess values, and pre-determined weight factors. A robust multiobjective optimization based on evolutionary algorithm proposed in this paper minimizes the error in yield stress and plastic strain ratio simultaneously and thereby overcomes the limitations in the approaches used before. The new approach is tested using Hill48 and Barlat89 yield criteria for five different materials from literature. The new approach is observed to improve the prediction capability of yield coefficients when compared to earlier approaches. The Pareto frontier obtained in the new approach can serve as a comparative tool to evaluate the accuracy of different yield criteria. 相似文献
993.
Eva Frühwald 《Holz als Roh- und Werkstoff》2007,65(6):411-418
In timber drying, mechanical properties may be changed due to treatment temperature and treatment duration. In general, when increasing the kiln temperature, drying time is decreased and some timber properties are negatively affected. In this study, the effect of different drying temperatures (80, 120 and 170 °C) on equilibrium moisture content and sorption rate, on bending strength and stiffness was investigated for Norway spruce and larch from four proveniences and the results were compared to those obtained for heat-treated wood (Thermowood ®). The experiments confirm earlier research that both treatment temperature and treatment duration affect the properties. High-temperature drying or treatment can be optimized for several applications, yielding strong but not so durable timber or vice versa. 相似文献
994.
Maria N. Criado Francisco J. Barba Ana Frígola Dolores Rodrigo 《Food and Bioprocess Technology》2014,7(5):1518-1525
The effect of different concentrations of Stevia rebaudiana Bertoni (Stevia) on polyphenoloxidase and peroxidase activities, antioxidant capacity and other bioactive compounds of a mixture of fruits, during 10 days of incubation at 10 and 37 °C, was studied. A significant decrease (p?<?0.05) in both enzyme activities was observed when Stevia was added, showing an inhibitory effect. Samples with a high Stevia concentration showed the highest total phenolic content and antioxidant capacity, whereas non-significant changes were found in the ascorbic acid and carotenoid contents of the samples with Stevia added. A negative significant dependence (p?<?0.05) during incubation was obtained between enzyme activity and antioxidant capacity, so that antioxidant capacity increases as enzyme activity decreases. Therefore, enzyme activity could be used as an indirect index of antioxidant capacity. The results clearly showed that Stevia had significant potential for use not only as a sweetener but also as a natural preservative agent. 相似文献
995.
Abstract: Addition of wheat bran to flours modifies their expansion properties after cooking extrusion. This can be attributed to changes in the melt shear viscosity at the die. The effect of wheat bran concentration added to achieve 2 levels of dietary fibers of 12. 6% and 24.4%, and process conditions on the shear viscosity of wheat flour was therefore assessed using an online twin‐slit rheometer. The shear viscosity measured at 30 s?1 ranged from 9.5 × 103 to 53.4 × 103 Pa s. Regardless of the process conditions and bran concentration, the extruded melts showed a pseudoplastic behavior with a power law index n ranging from 0.05 to 0.27. Increasing the barrel temperature of the extruder from 120 to 180 °C, the water content from 18% to 22% or the screw speed from 400 to 800 rpm significantly decreased the melt shear viscosity at the extruder exit. The addition of bran significantly increased the melt shear viscosity only at the highest bran concentration. The effect was process condition dependant. Mathematical interpretations, based upon observations, of the experimental data were carried out. They can be used to predict the effect of the process conditions on the melt shear viscosity at the die of extruded wheat flour with increasing bran concentration. The viscosity data will be applied in future works to study the expansion properties of extruded wheat flour supplemented with bran. Practical Application: Incorporation of wheat bran, a readily available and low cost by‐product, in extruded puffed foods is constrained due to its negative effect on the product texture. Understanding the effect of wheat bran on rheological properties of extruded melts, driving the final product properties, is essential to provide solutions to the food industry and enhance its use. 相似文献
996.
Capuano F Bond NJ Gatto L Beaudoin F Napier JA Benvenuto E Lilley KS Baschieri S 《Analytical chemistry》2011,83(24):9267-9272
Oil bodies (OBs) are plant cell organelles that consist of a lipid core surrounded by a phospholipid monolayer embedded with specialized proteins such as oleosins. Recombinant proteins expressed in plants can be targeted to OBs as fusions with oleosin. This expression strategy is attractive because OBs are easily enriched and purified from other cellular components, based on their unique physicochemical properties. For recombinant OBs to be a potential therapeutic agent in biomedical applications, it is necessary to comprehensively analyze and quantify both endogenous and heterologously expressed OB proteins. In this study, a mass spectrometry (MS)-based method was developed to accurately quantify an OB-targeted heterologously expressed fusion protein that has potential as a therapeutic agent. The effect of the chimeric oleosin expression upon the OB proteome in transgenic plants was also investigated, and the identification of new potential OB residents was pursued through a variety of liquid chromatography (LC)-MS/MS approaches. The results showed that the accumulation of the fusion protein on OBs was low. Moreover, no significant differences in the accumulation of OB proteins were revealed between transgenic and wild-type seeds. The identification of five new putative components of OB proteome was also reported. 相似文献
997.
In situ tracking of enzymatic breakdown of starch granules by synchrotron UV fluorescence microscopy
Tawil G Jamme F Réfrégiers M Viksø-Nielsen A Colonna P Buléon A 《Analytical chemistry》2011,83(3):989-993
Synchrotron UV fluorescence microscopy was used for the first time to visualize the adsorption and diffusion of an enzyme while degrading a solid substrate. The degradation pathway of single starch granules by two amylases, optimized for biofuel production and industrial starch hydrolysis, was followed by tryptophan fluorescence (excitation at 280 nm, emission filter at 300-400 nm) and visible light imaging. Thus, both the adsorption of enzyme onto starch granules at 283 nm resolution and the resulting morphological changes were recorded at different stages of hydrolysis. It is the first time that amylases were localized on starch without staining or adding a fluorescent probe at such high resolution. This technique presents a very high potential for imaging proteins in complex systems. Its sensitivity was demonstrated by the detection of GBSS (the granular bound starch synthase) at high recording times, GBSS being present at very low levels in maize starch granules. 相似文献
998.
An analysis of 2000–2007 single vehicle rollover fatalities in three Australian states was carried out using data from the Australian National Coroners Information System. In this paper, successive selection criteria were applied to the initial dataset to analyse:
-
Occupant fatalities in single passenger car crashes (1743 cases), -
Occupant fatalities in single passenger car rollovers (474 cases),
999.
Monroe MR Reddington AP Collins AD LaBoda C Cretich M Chiari M Little FF Unlü MS 《Analytical chemistry》2011,83(24):9485-9491
Using a microarray platform for allergy diagnosis allows for testing of specific IgE sensitivity to a multitude of allergens, while requiring only small volumes of serum. However, variation of probe immobilization on microarrays hinders the ability to make quantitative, assertive, and statistically relevant conclusions necessary in immunodiagnostics. To address this problem, we have developed a calibrated, inexpensive, multiplexed, and rapid protein microarray method that directly correlates surface probe density to captured labeled secondary antibody in clinical samples. We have identified three major technological advantages of our calibrated fluorescence enhancement (CaFE) technique: (i) a significant increase in fluorescence emission over a broad range of fluorophores on a layered substrate optimized specifically for fluorescence; (ii) a method to perform label-free quantification of the probes in each spot while maintaining fluorescence enhancement for a particular fluorophore; and (iii) a calibrated, quantitative technique that combines fluorescence and label-free modalities to accurately measure probe density and bound target for a variety of antibody-antigen pairs. In this paper, we establish the effectiveness of the CaFE method by presenting the strong linear dependence of the amount of bound protein to the resulting fluorescence signal of secondary antibody for IgG, β-lactoglobulin, and allergen-specific IgEs to Ara h 1 (peanut major allergen) and Phl p 1 (timothy grass major allergen) in human serum. 相似文献
1000.
Digital in-line holography (DIH) with a divergent beam is used to measure size and concentration of cavitation bubbles (6-100 μm) in hydrodynamic facilities. A sampling probe is directly inserted in the cavitation tunnel, and the holograms of the bubbles are recorded through a transparent test section specially designed for DIH measurements. The recording beam coming from a fiber-coupled laser diode illuminates the sample volume, and holograms are recorded by a CMOS camera. From each hologram, the sampling volume can be reconstructed slice by slice by applying a wavelet-based reconstruction method. Because of the geometry of the recording beam, a magnification ratio must be introduced for recovering the 3D location and size of each bubble. The method used for processing holograms recorded in such a configuration is presented. Then, statistical results obtained from 5000 holograms recorded under different pressures in the cavitation tunnel are compared and discussed. 相似文献