Nine-analyte detection using an array-based biosensor

A fluorescence-based multianalyte immunosensor has been developed for simultaneous analysis of multiple samples. While the standard 6 x 6 format of the array sensor has been used to analyze six samples for six different analytes, this same format has the potential to allow a single sample to be tested for 36 different agents. The method described herein demonstrates proof of principle that the number of analytes detectable using a single array can be increased simply by using complementary mixtures of capture and tracer antibodies. Mixtures were optimized to allow detection of closely related analytes without significant cross-reactivity. Following this facile modification of patterning and assay procedures, the following nine targets could be detected in a single 3 x 3 array: Staphylococcal enterotoxin B, ricin, cholera toxin, Bacillus anthracis Sterne, Bacillus globigii, Francisella tularensis LVS, Yersiniapestis F1 antigen, MS2 coliphage, and Salmonella typhimurium. This work maximizes the efficiency and utility of the described array technology, increasing only reagent usage and cost; production and fabrication costs are not affected.     

A Local Database Manager for Engineering Analysis Systems

Abstract: A local database manager (LDBM) is presented. It is a software development tool consisting of a set of easy-to-use in-core data management subroutines written in FORTRAN 77. One of the main motivations for the development of this LDBM was to enhance the eflcient use of core memory which seems to be obvious with respect to a PC programming environment. However, even on virtual machines, high performance achieved by minimizing page faults is strongly based on a sophisticated database management system (DBMS). In order to accomplish this objective, the LDBM allows the use of different data types according to the requirements of the actual situation and to the existing programming environment. Integer, short integer, real, double precision, complex, double complex and character data can be handled. Moreover, a two-level hierarchical data structure is used which groups related data in a different and more economic way rather than by means of increasing the number of levels in the DIMENSION statement. Of course, old, no longer used data may be deleted, leaving room for the allocation of new data. Using a technique of 'flexible garbage collection', i.e. the ensuing gap after the deletion of a data block is not automatically closed, the time consumed by the LDBM during a deletion operation is actually not affected by the length of data blocks.     

"Choice processing in emotionally difficult decisions": Correction.

Reports an error in the article "Choice Processing in Emotionally Difficult Decisions' by Mary Frances Luce, James R. Bettman, and John W. Payne (Journal of Experimental Psychology: Learning, Memory, and Cognition, 1997, Vol. 23, No. 2, pp. 384–405; see record 1997-03378-007). In Table 7 on page 396, the high conflict/low trade-off difficulty mean for the PATTERN variable was printed as -0.13, but the actual mean is 0.13. A portion of the original abstract follows: Extends the standard effort-accuracy approach to explaining task influences on decision processing by arguing that coping goals will interact with effort-minimization goals for negatively emotion-laden decision tasks. These coping goals may involve both a desire to process in a thorough, accurate manner and a desire to avoid particularly distressing aspects of processing. The authors hypothesized and found in 3 experiments that decision processing under increasing negative emotion both becomes more extensive and proceeds more by focusing on one attribute at a time. In particular, increased negative emotion leads to more attribute-based processing at the beginning of the decision process. The results are inconsistent with views that negative emotion acts only as an incentive or only as a source of decision complexity. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Random model of evaporation of oil at sea

When oil is spilled at sea, a portion of it evaporates. The amount depends upon the oil's physical properties and environmental conditions. This paper explores the effect of a random environment on the evaporation process. A random model is developed to predict the residual volume of oil as a function of elapsed time, initial oil volume and its physical properties, and random environmental factors (wind speed and ambient temperature). A simple example illustrates application of the model. Also, a simulation illustrates the effects of random wind speeds on the residual volume and area of the oil slick as a function of time.     

Dynamic accommodation response in the presence of astigmatism

It has been suggested that in the presence of astigmatism some individuals make cyclic changes in focus over the astigmatic interval to obtain better visual performance. The aim in the present study was to identify such cyclic accommodative behavior and to characterize the variability of the response in the presence of astigmatism. The dynamic accommodation response in the presence of induced astigmatism was recorded objectively with an infrared optometer in seven young adults. Astigmatism led directly to increased accommodative variability in certain individuals. In two of seven participants there was evidence for aperiodic cyclic accommodative responses between different portions of the astigmatic interval. However, the amplitude of these tracking responses was much smaller than the astigmatic interval.     

Two-Color Capillary Electrophoresis with On-Column Excitation and Wave-Guide Based Fluorescent Detection

An optical wave-guide based two-color capillary electrophoresis laser induced fluorescence (CE-LIF) instrument is described. The wave-guide based approach allows for on column excitation and detection with two-color discrimination. The instrument is designed to allow either electrokinetic or hydrodynamic injections. In its present configuration, the attainable limit of detection (LOD, S/N = 3) was 50 X 10⁻²¹ moles of fluorescein with a 488-nm excitation source. This study was designed to test the instrument design for applications in protein analyses. Fluorescent dyes with two different wavelengths were simultaneously separated and detected as were complexes formed by labeled antibodies to NFκB p65 and cdc2p34. Quantification of both proteins in THP-1 cell lysates performed using this approach illustrates a rapid screening application of this instrument.