Capacitance DNA bio-chips improved by new probe immobilization strategies

Label-free DNA detection plays a crucial role in developing point-of-care biochips. Capacitance detection is a promising technology for label-free detection. However, data published in literature often show evident time drift, large standard deviation, scattered data points, and poor reproducibility. To address these problems, mercapto-hexanol or similar alkanethiols are usually considered as blocking agents. The aim of the present paper is to investigate new blocking agents to further improve DNA probe surfaces. Data from AFM, SPR, florescence microscopy, and capacitance measurements are used to investigate new lipoate and ethylene-glycol molecules. The new surfaces offer further improvements in terms of diminished detection errors. Film structures are investigated at the nano-scale to justify the detection improvements in terms of probe surface quality. This study demonstrates the superiority of lipoate and ethylene-glycol molecules as blocking candidates when immobilizing molecular probes onto spot surfaces in label-free DNA biochip.     

Suppressed Lattice Disorder for Large Emission Enhancement and Structural Robustness in Hybrid Lead Iodide Perovskite Discovered by High-Pressure Isotope Effect

The soft nature of organic–inorganic halide perovskites renders their lattice particularly tunable to external stimuli such as pressure, undoubtedly offering an effective way to modify their structure for extraordinary optoelectronic properties. Here, using the methylammonium lead iodide as a representative exploratory platform, it is observed that the pressure-driven lattice disorder can be significantly suppressed via hydrogen isotope effect, which is crucial for better optical and mechanical properties previously unattainable. By a comprehensive in situ neutron/synchrotron-based analysis and optical characterizations, a remarkable photoluminescence (PL) enhancement by threefold is convinced in deuterated CD₃ND₃PbI₃, which also shows much greater structural robustness with retainable PL after high peak-pressure compression–decompression cycle. With the first-principles calculations, an atomic level understanding of the strong correlation among the organic sublattice and lead iodide octahedral framework and structural photonics is proposed, where the less dynamic CD₃ND₃⁺ cations are vital to maintain the long-range crystalline order through steric and Coulombic interactions. These results also show that CD₃ND₃PbI₃-based solar cell has comparable photovoltaic performance as CH₃NH₃PbI₃-based device but exhibits considerably slower degradation behavior, thus representing a paradigm by suggesting isotope-functionalized perovskite materials for better materials-by-design and more stable photovoltaic application.     

Pectic Galactan Polysaccharide-Based Gene Delivery System for Targeting Neuroinflammation

Treating neuroinflammation-related injuries and disorders through manipulation of neuroinflammation functions is being heralded as a new therapeutic strategy. In this study, a novel pectic galactan (PG) polysaccharide based gene therapy approach is developed for targeting reactive gliosis in neuroinflammation. Galectin-3 (Gal-3) is a cell protein with a high affinity to β-galactoside sugars and is highly expressed in reactive gliosis. Since PG carries galactans, it can target reactive gliosis via specific carbohydrate interaction between galactan and Gal-3 on the cell membrane, and therefore can be utilized as a carrier for delivering genes to these cells. The carrier is synthesized by modifying quaternary ammonium groups on the PG. The resulting quaternized PG (QPG) is found to form complexes with plasmid DNA with a mean diameter of 100 nm and have the characteristics required for targeted gene therapy. The complexes efficiently condense large amounts of plasmid per particle and successfully bind to Gal-3. The in vivo study shows that the complexes are biocompatible and safe for administration and can selectively transfect reactive glial cells of an induced cortical lesion. The results confirm that this PG-based delivery system is a promising platform for targeting Gal-3 overexpressing neuroinflammation cells for treating neuroinflammation-related injuries and neurodegenerative diseases.     

A polyfluoroalkyl imidazolium ionic liquid as iodide ion source in dye sensitized solar cells

We report on the use of a fluorinated imidazolium ionic liquid as a source of iodide ions in solvent-based electrolytes for DSSCs. Efficient dye regeneration and fast charge transport in the fluorinated electrolyte result in an overall improvement of the device performances compared to conventional hydrogenated ionic liquids.     

Transport layer enhancements on a satellite‐based mobile broadband system for high‐speed trains

The great diffusion of wireless portable and handheld devices, along with the growing availability of applications on the Internet, led to a significant increase in the demand of access. Users require to continue business or private activities when moving, with performance at least comparable with that experienced over fixed networks. A very interesting scenario encompasses broadband and uninterrupted access over high‐speed trains. Developments of new telecommunication standards as well as innovative network architectures give the potential to fully match the aforementioned requirements. Nevertheless, there is a significant number of issues, especially related to system integration, mobility management, and propagation channel conditions, that need a careful analysis with the aim to support the design of real systems. This paper deals with this challenging scenario proposing a satellite‐based architecture in which DVB‐S2 and DVB‐RCS standards, MIPv6 at layer 3 (for handover management), and an enhanced TCP version at layer 4, named TCP Noordwijk, allow to achieve good performance. An NS‐2 enhanced simulator has supported the whole analysis. Results show the effectiveness of the proposed scheme against different modulation and coding configurations and compared with a selected set of TCP‐enhanced versions. Copyright © 2012 John Wiley & Sons, Ltd.     

Robust security framework for DVB‐RCS satellite networks (RSSN)

Satellites represent a solution for Internet access in locations with no other telecom infrastructures, for example, on high mobility platforms such as planes, ships or high‐speed trains, or for disaster recovery applications. However, due to peculiar characteristics, satellite networks are prone to different security threats. In this paper, we introduce a novel, robust security architecture for securing digital video broadcasting‐return channel via satellite satellite networks, inspired by the robust security mechanism available in the Institute of Electrical and Electronic Engineers (IEEE) 802.11i wireless local area network. We propose an efficient authentication and key management mechanism, which is exploited through three round‐trips only, demonstrating that it is as secure as IEEE 802.11i. Furthermore, the simulation results show that the proposed security framework needs a very small data overhead and shows better performance than internet protocol security (IPSec), which is commonly used as an end‐to‐end security solution over internet protocol satellite networks. Copyright © 2015 John Wiley & Sons, Ltd.     

Reduced Impact of Induced Gate Noise on Inductively Degenerated LNAs in Deep Submicron CMOS Technologies

Designers of radio-frequency inductively-degenerated CMOS low-noise-amplifiers have usually not followed the guidelines for achieving minimum noise figure. Nonetheless, state-of-the-art implementations display noise figure values very close to the theoretical minimum. In this paper, we point out that this is due to the effect of the parasitic overlap capacitances in the MOS device. In particular, we show that overlap capacitances lead to a significant induced-gate-noise reduction, especially when deep sub-micron CMOS processes are used.Paolo Rossi was born in Milan, Italy, in 1975. He received the Laurea degree (summa cum laude) in electrical engineering from the University of Pavia, Pavia, Italy, in 2000, where he is currently working toward the Ph.D. degree. His research interests are in the field of analog integrated circuits for wireless transceivers in CMOS and BiCMOS technology, with particular focus on the analysis and design of LNA and mixer for multi-standard applications.Francesco Svelto received the Laurea and Ph.D. degrees in electrical engineering from the University of Pavia, Pavia, Italy, in 1991 and 1995, respectively. From 1996 to 1997, he held a grant from STMicroelectronics to design CMOS RF circuits. In 1997, he was appointed Assistant Professor at the University of Bergamo, Italy, and in 2000, he joined the University of Pavia, where he is an Associate Professor. His current research interests are in the field of RF design and high-frequency integrated circuits for telecommunications. Dr. Svelto has been a member of the technical program committee of the IEEE Custom Integrated Circuits Conference since 2000 and the Bipolar/BiCMOS Circuits and Technology Meeting (BCTM) since 2003, and the European Solid State Circuits Conference in 2002. He served as Guest Editor of the March 2003 special issue of the IEEE Journal of Solid-State Circuits, of which he is currently an Associate Editor.Andrea Mazzanti was born in Modena (Italy) in 1976. He received the Laurea degree (summa cum Laude) in Electrical Engineering from the University of Modena and Reggio Emilia, Modena, Italy in 2001. Since 2001 he is pursuing his PhD in Electrical Engineering at University of Modena and Reggio Emilia, Italy. His major research interest are modelling of microwave semiconductor devices and design of CMOS RF integrated circuits, with particular focus on low noise oscillators and analog frequency dividers. During the summer of 2003 he was with Agere Systems, Allentown, PA as an internship student, working on the design of an highly integrated CMOS FM transmitter.Pietro Andreani received the M.S.E.E. from the University of Pisa, Italy, in 1988. He joined the Dept. of Applied Electronics, Lund University, Sweden, in 1990, where he contributed to the development of software tools for digital ASIC design. After working at the Dept. of Applied Electronics, University of Pisa, as a CMOS IC designer during 1994, he rejoined the Dept. of Applied Electronics in Lund as an Associate Professor, where he was responsible for the analog IC course package between 1995 and 2001, and where he received the Ph.D. degree in 1999. He is currently a Professor at the Center for Physical Electronics, ØrstedDTU, Technical University of Denmark, Kgs. Lyngby, Denmark, with analog/RF CMOS IC design as main research field.     

Stimulation of the activity of the IAA-oxidising enzyme - system in Lens culinaris roots by dalapon and perfluidone

The activity of IAA-oxidising enzymes from Lens roots is enhanced in vitro by dalapon (2,2-dichloropropionic acid) and perfluidone (1,1,1 -trifluoro-N-[2-methyl-4-(phenylsulphonyl)phenyl]methanesulphonamide). When concentrations are greater than 10^?8 M, both these herbicides act as activators. In vivo higher levels of the enzymatic activity are obtained when seed germination is carried out in the presence of 10^?5 M perfluidone or 10^?3 M dalapon. Gas-liquid chromatography is used for IAA determination.