Comparison of virulence and antibiotic resistance genes of food poisoning outbreak isolates of Staphylococcus aureus with isolates obtained from bovine mastitis milk and pig carcasses

Staphylococcus aureus is the etiological agent in a variety of infections in humans and livestock and produces enterotoxins leading to staphylococcal food poisoning (SFP), one of the most prevalent foodborne intoxication diseases worldwide. Pork and bovine milk are considered possible sources of SFP because pig skin is often colonized by S. aureus and bovine mastitis caused by S. aureus is common, but conclusive data are limited. The objective of the present study was to compare S. aureus isolates associated with cases of SFP with isolates obtained from bovine mastitis milk and pig carcasses. DNA microarray analysis and spa gene typing were performed with 100 S. aureus isolates: 20 isolates related to outbreaks of SFP in humans, 39 isolates obtained from pig carcasses, and 41 isolates collected from bovine mastitis milk. No overlap in spa types was observed for SFP isolates (t008, t015, t018, t024, t056, t084, t279, t377, t383, t648, t733, t912, t1239, t1270, t4802, and t6969) and isolates gathered from milk or pork. The porcine isolates were assigned to t034, t208, t337, t524, t899, t1939, t2922, t2971, t4475, and t7006, and the bovine isolates belonged to t267, t524, t529, t1403, t2953, t7007, t7008, and t7013. Comparison of microarray profiles revealed similar virulence gene patterns for isolates collected from the same host (pigs or cattle) but few similarities between SFP isolate profiles and the profiles of isolates obtained from bovine mastitis milk and pig carcasses. Although only some bovine and porcine isolates possessed the β-lactamase gene blaZ (milk, 24%; pork, 28%), significantly higher numbers of SFP isolates contained blaZ (90%). Investigations of these isolates provided no evidence that pork or bovine mastitis milk represent common sources of SFP.     

Global food markets,trade and the cost of climate change adaptation

Achieving food security in the face of climate change is a major challenge for humanity in the 21st century but comprehensive analyses of climate change impacts, including global market feedbacks are still lacking. In the context of uneven impacts of climate change across regions interconnected through trade, climate change impact and adaptation policies in one region need to be assessed in a global framework. Focusing on four Eastern Asian countries and using a global integrated modeling framework we show that i) once imports are considered, the overall climate change impact on the amount of food available could be of opposite sign to the direct domestic impacts and ii) production and trade adjustments following price signals could reduce the spread of climate change impacts on food availability. We then investigated how pressure on the food system in Eastern Asia could be mitigated by a consumer support policy. We found that the costs of adaptation policies to 2050 varied greatly across climate projections. The costs of consumer support policies would also be lower if only implemented in one region but market price leakage could exacerbate pressure on food systems in other regions. We conclude that climate adaptation should no longer be viewed only as a geographically isolated local problem.     

Ethyl Hydroxyethyl Cellulose—A Biocompatible Polymer Carrier in Blood

The biocompatibility of carrier nanomaterials in blood is largely hampered by their activating or inhibiting role on the clotting system, which in many cases prevents safe intravascular application. Here, we characterized an aqueous colloidal ethyl hydroxyethyl cellulose (EHEC) solution and tested its effect on ex vivo clot formation, platelet aggregation, and activation by thromboelastometry, aggregometry, and flow cytometry. We compared the impact of EHEC solution on platelet aggregation with biocompatible materials used in transfusion medicine (the plasma expanders gelatin polysuccinate and hydroxyethyl starch). We demonstrate that the EHEC solution, in contrast to commercial products exhibiting Newtonian flow behavior, resembles the shear-thinning behavior of human blood. Similar to established nanomaterials that are considered biocompatible when added to blood, the EHEC exposure of resting platelets in platelet-rich plasma does not enhance tissue thromboplastin- or ellagic acid-induced blood clotting, or platelet aggregation or activation, as measured by integrin α_IIbβ₃ activation and P-selectin exposure. Furthermore, the addition of EHEC solution to adenosine diphosphate (ADP)-stimulated platelet-rich plasma does not affect the platelet aggregation induced by this agonist. Overall, our results suggest that EHEC may be suitable as a biocompatible carrier material in blood circulation and for applications in flow-dependent diagnostics.     

Towards Certificate-Based Authentication for Future Mobile Communications

Certificate-based authentication of parties provides a powerful means for verifying claimed identities, since communicating partners do not have to exchange secrets in advance for authentication. This is especially valuable for roaming scenarios in future mobile communications where users authenticate to obtain network access—service access may potentially be based thereon in integrated approaches—and where the number of access network providers and Internet service providers is expected to increase considerably. When dealing with certificates, one must cope with the verification of complete certificate paths for security reasons. In mobile communications, additional constraints exist under which this verification work is performed. These constraints make verification more difficult when compared to non-mobile contexts. Mobile devices may have limited capacity for computation and mobile communication links may have limited bandwidth. In this paper, we propose to apply PKI servers—such as implemented at FhG-SIT—that allow the delegation of certificate path validation in order to speed up verification. Furthermore, we propose a special structure for PKI components and specific cooperation models that force certificate paths to be short, i.e., the lenghts of certificate paths are upper-bounded to certain small values depending on the conditions of specific cases. Additionally, we deal with the problem of users who do not have Internet access during the authentication phase. We explain how we solved this problem and show a gap in existing standards.     

Autonomic nervous function during haemodialysis assessed by spectral analysis of heart-rate variability

To investigate the effect of neuronal differentiation on the capacity of antisense oligonucleotides (AS-ODNs) to suppress the production of acetylcholinesterase (AChE) in rat pheochromocytoma cells, we tested seven 3'-phosphorothioated AS-ODNs targeted to ACHEmRNA and two control ODNs. Three different administration protocols were used: oligonucleotides were added at 1 microM for 24 hours to nondifferentiated PC12 cells, together with nerve growth factor (NGF) or 24 hours following NGF-induced cholinergic differentiation. The content of free thiol groups in lysed cells was measured to evaluate cell number, therefore, survival, and the rate of acetylthiocholine hydrolysis was the measure of AChE activity. Among nondifferentiated cells, over 95% survived treatment with 8 of 9 of the ODNs. Moreover, two AS-ODN suppressed AChE activity in non-differentiated PC12 cells by 16%-20% as compared with 10% suppression by control ODNs (P < or = 0.01). When added concurrently with NGF, one other AS-ODN suppressed AChE activity significantly better (28%) than the control ODNs (16%). Moreover, when added following NGF treatment, which induced a significant increase in AChE activity, four different AS-ODNs but not the control ODNs suppressed 20%-35% of the enhanced AChE activity (p < or = 0.01). Reduced levels of AChE mRNA but no difference in actin mRNA levels were observed by following the kinetics of RT-PCR amplification in differentiated PC12 cells treated with these four AS-ODNs, as compared with control cells. Our findings demonstrate a differentiation-related increase in the susceptibility of PC12 cells to inhibition by specific AS-ODNs, suggesting the use of this model system to select AS-ODNs for suppression of AChE levels in the treatment of neurodegenerative diseases associated with cholinergic malfunction.     

Synthesis of carbon nanotubes with and without catalyst particles

The initial development of carbon nanotube synthesis revolved heavily around the use of 3d valence transition metals such as Fe, Ni, and Co. More recently, noble metals (e.g. Au) and poor metals (e.g. In, Pb) have been shown to also yield carbon nanotubes. In addition, various ceramics and semiconductors can serve as catalytic particles suitable for tube formation and in some cases hybrid metal/metal oxide systems are possible. All-carbon systems for carbon nanotube growth without any catalytic particles have also been demonstrated. These different growth systems are briefly examined in this article and serve to highlight the breadth of avenues available for carbon nanotube synthesis.     

Spherical polymer brushes with vinylimidazolium-type poly(ionic liquid) chains as support for metallic nanoparticles

We present here the synthesis of spherical polyelectrolyte brushes by photo-emulsion polymerization with a solid polystyrene core (diameter ～ 100 nm) onto which chains of vinylimidazolium-type poly(ionic liquid)s have been densely grafted. The as-synthesized brush particles were employed as nanoreactors for the generation and immobilization of metal nanoparticles. Transmission electron microscopy characterization shows that Au and Pd nanoparticles with diameter of 2.1 ± 0.2 nm and 2.5 ± 0.3 nm are homogeneously embedded inside the PIL brushes, respectively. The study of swelling behavior of the brush particles before and after metal deposition indicates obviously ion-specific effect. The composite particles exhibit a long-term colloidal stability in aqueous solutions as well as in ionic liquids. Catalytic activity of the as-synthesized metal nanocomposite particles is investigated by using the reduction of 4-nitrophenol with NaBH₄ as a model reaction, which can be compared directly with reported systems. In addition, it is found that the rate constant k_app of PIL-metal nanocatalyst could be modulated by salt concentration.     

The effect of betaine on in vitro fermentation of carbohydrate and protein combinations under osmotic stress in pigs

BACKGROUND: There is evidence that dietary betaine might improve nutrient digestibilities in pigs due to its osmoprotective properties. This in vitro study was performed to assess the potential of supplemented betaine sources to affect fermentation characteristics of different carbohydrate and protein combinations, under conditions of osmotic stress. In vitro fermentation was performed by use of the modified Hohenheim gas test with pig faeces as microbial inoculum. RESULTS: At the end of in vitro fermentation under osmotic stress conditions, differences (P < 0.05) in gas production, ammonia concentration, and concentration of short‐chain fatty acids were observed for the different carbohydrates, while for the two proteins only differences (P < 0.05) in ammonia and short‐chain fatty acid concentration were obtained. Supplementation of a native betaine source rather than addition of pure betaine increased propionic acid (P < 0.05) and butyric acid (P < 0.05) production. CONCLUSION: These results suggest that only supplementation of a native betaine source but not of pure betaine products affects microbial fermentation under osmotic stress. Furthermore, both the carbohydrate and protein source play an important role in modifying microbial activity under osmotic stress conditions. It can also be concluded that the modified Hohenheim gas test is suitable to scrutinise fermentation activity of different assay substrates under osmotic stress. Copyright © 2012 Society of Chemical Industry     

Immune Cells Invade the Collateral Circulation during Human Stroke: Prospective Replication and Extension

It remains unclear if principal components of the local cerebral stroke immune response can be reliably and reproducibly observed in patients with acute large-vessel-occlusion (LVO) stroke. We prospectively studied a large independent cohort of n = 318 consecutive LVO stroke patients undergoing mechanical thrombectomy during which cerebral blood samples from within the occluded anterior circulation and systemic control samples from the ipsilateral cervical internal carotid artery were obtained. An extensive protocol was applied to homogenize the patient cohort and to standardize the procedural steps of endovascular sample collection, sample processing, and laboratory analyses. N = 58 patients met all inclusion criteria. (1) Mean total leukocyte counts were significantly higher within the occluded ischemic cerebral vasculature (I) vs. intraindividual systemic controls (S): +9.6%, I: 8114/µL ± 529 vs. S: 7406/µL ± 468, p = 0.0125. (2) This increase was driven by neutrophils: +12.1%, I: 7197/µL ± 510 vs. S: 6420/µL ± 438, p = 0.0022. Leukocyte influx was associated with (3) reduced retrograde collateral flow (R² = 0.09696, p = 0.0373) and (4) greater infarct extent (R² = 0.08382, p = 0.032). Despite LVO, leukocytes invade the occluded territory via retrograde collateral pathways early during ischemia, likely compromising cerebral hemodynamics and tissue integrity. This inflammatory response can be reliably observed in human stroke by harvesting immune cells from the occluded cerebral vascular compartment.