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81.
D Finzi M Hermankova T Pierson LM Carruth C Buck RE Chaisson TC Quinn K Chadwick J Margolick R Brookmeyer J Gallant M Markowitz DD Ho DD Richman RF Siliciano 《Canadian Metallurgical Quarterly》1997,278(5341):1295-1300
The hypothesis that quiescent CD4+ T lymphocytes carrying proviral DNA provide a reservoir for human immunodeficiency virus-type 1 (HIV-1) in patients on highly active antiretroviral therapy (HAART) was examined. In a study of 22 patients successfully treated with HAART for up to 30 months, replication-competent virus was routinely recovered from resting CD4+ T lymphocytes. The frequency of resting CD4+ T cells harboring latent HIV-1 was low, 0.2 to 16.4 per 10(6) cells, and, in cross-sectional analysis, did not decrease with increasing time on therapy. The recovered viruses generally did not show mutations associated with resistance to the relevant antiretroviral drugs. This reservoir of nonevolving latent virus in resting CD4+ T cells should be considered in deciding whether to terminate treatment in patients who respond to HAART. 相似文献
82.
Arif Sasongko Amer Baghdadi Frederic Rousseau Ahmed Amine Jerraya 《Design Automation for Embedded Systems》2003,8(2-3):155-171
Hardware/software covalidation is becoming one of the most critical issues in current System-on-Chip (SoC) design. Nowadays, covalidation is usually performed by cosimulation which is slow and lacks accuracy. The other alternative is to build a hardware prototype specific to the application. However, this alternative is expensive in terms of time, man-power, and cost. As SoCs increase in complexity, validation becomes more and more difficult, time consuming and error prone. Thus, a new approach for covalidation is inescapable. In this paper, we present a novel efficient prototyping approach for complex SoC covalidation. The proposed approach enables systematic prototyping of embedded applications on a reconfigurable platform. The process starts from the RT level model of the application. The application and the reconfigurable platform have to be adapted to obtain the prototype. We decompose the prototyping process into four steps, in order to match the application and the platform. Besides, we propose adapted solutions to deal with constraints typically encountered in existing reconfigurable platforms. The main advantages of this method are: fast and accurate validation, systematic prototyping flow, and large application field. Prototyping of a subset of VDSL using the ARM Integrator platform illustrates the effectiveness of our approach. 相似文献
83.
Jeong Whan Yoon Kwansoo Chung Farhang Pourboghrat Frederic Barlat 《International Journal of Mechanical Sciences》2006,48(12):1416-1428
The conventional practice to predict preform shapes in hydroformimg processes based on finite-element analysis and/or experiment is an iterative procedure and requires many trials. In this paper, a computationally efficient direct design method, which effectively improves the design procedure, was introduced. The direct design method based on ideal forming theory, which was successfully applied for the design of flat blanks for stamping processes, was extended for the design of non-flat preform for tube hydroforming processes. A preform optimization methodology for non-flat blank solutions was proposed based on the penalty constraint method for the cross-sectional shape and length of a tube. The hybrid membrane/shell method was employed to capture thickness effect while maintaining membrane formulation in the ideal forming theory. Several classes of examples were analyzed to verify the current formulation. 相似文献
84.
Massarin S Beaudouin R Zeman F Floriani M Gilbin R Alonzo F Pery AR 《Environmental science & technology》2011,45(9):4151-4158
Recent studies have investigated chronic toxicity of waterborne depleted uranium on the life cycle and physiology of Daphnia magna. In particular, a reduction in food assimilation was observed. Our aims here were to examine whether this reduction could fully account for observed effects on both growth and reproduction, for three successive generations, and to investigate through microscope analyses whether this reduction resulted from direct damage to the intestinal epithelium. We analyzed data obtained by exposing Daphnia magna to uranium over three successive generations. We used energy-based models, which are both able to fit simultaneously growth and reproduction and are biologically relevant. Two possible modes of action were compared - decrease in food assimilation rate and increase in maintenance costs. In our models, effects were related either to internal concentration or to exposure concentration. The model that fitted the data best represented a decrease in food assimilation related to exposure concentration. Furthermore, observations of consequent histological damage to the intestinal epithelium, together with uranium precipitates in the epithelial cells, supported the assumption that uranium has direct effects on the digestive tract. We were able to model the data in all generations and showed that sensitivity increased from one generation to the next, in particular through a significant increase of the intensity of effect, once the threshold for appearance of effects was exceeded. 相似文献
85.
We report on the fabrication of covalently crosslinked and amine-reactive hollow microcapsules using 'reactive' layer-by-layer assembly to deposit thin polymer films on sacrificial microparticle templates. Our approach is based on the alternating deposition of layers of a synthetic polyamine and a polymer containing reactive azlactone functionality. Multilayered films composed of branched poly(ethylene imine) (BPEI) and poly(2-vinyl-4,4-dimethylazlactone) (PVDMA) were fabricated layer-by-layer on the surfaces of calcium carbonate and glass microparticle templates. After fabrication, these films contained residual azlactone functionality that was accessible for reaction with amine-containing molecules. Dissolution of the calcium carbonate or glass cores using aqueous ethylenediamine tetraacetic acid (EDTA) or hydrofluoric acid (HF), respectively, led to the formation of hollow polymer microcapsules. These microcapsules were robust enough to encapsulate and retain a model macromolecule (FITC-dextran) and were stable for at least 22 hours in high ionic strength environments, in low and high pH solutions, and in several common organic solvents. Significant differences in the behaviors of capsules fabricated on CaCO(3) and glass cores were observed and characterized using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). Whereas capsules fabricated on CaCO(3) templates collapsed upon drying, capsules fabricated on glass templates remained rigid and spherical. Characterization using EDS suggested that this latter behavior results, at least in part, from the presence of insoluble metal fluoride salts that are trapped or precipitate within the walls of capsules after etching of the glass cores using HF. Our results demonstrate that the assembly of BPEI/PVDMA films on sacrificial templates can be used to fabricate reactive microcapsules of potential use in a wide range of fields, including catalysis, drug and gene delivery, imaging, and biomedical research. 相似文献
86.
This paper considers pre-impact vehicle maneuvers and analyzes the resulting driver motion from their comfort seating position. Part I of this work consists of analyzing the driver behavior during a crash. The study is conducted using the LAMIH driving simulator and involves 76 participants. The emergency situation is created by a truck emerging from behind a tractor on the opposite side of the road and tearing along the participant. The driver positioning throughout the simulation is recorded via five video cameras allowing view of the front scene, the driver face, feet and pedals, hands on the steering wheel and global lateral view. Data related to braking force, seat pressure, muscular activity for major groups of muscles and actions on the steering wheel are also collected.The typical response to this type of emergency event is to brace rearward into the seat and to straighten the arms against the steering wheel, or, to swerve to attempt to avoid the impacting vehicle. While turning the steering wheel, the forearm can be directly positioned on the airbag module at time of crash which represents a potential injurious situation.These positions are used in Part II to determine scenario of positions for numerical simulation of a frontal collision. 相似文献
87.
Avinash Hariharan Phil Goldberg Frederic Schell Ute Hempel Friedrich Striggow Martin Hantusch Mariana Medina-Sánchez Andrés F. Lasagni Annett Gebert 《Advanced functional materials》2024,34(8):2310607
This study explores the enhancement of biocompatible titanium-based implants through surface functionalization for improved bone healing. Specifically, a near-beta type Ti-13Nb-13Zr alloy is 3D printed using laser powder bed fusion and subsequently textured using nanosecond (ns) and picosecond (ps) direct laser interference patterning (DLIP) to create single-scale and multi-scale surface textures. On these textures, the cell behavior, morphology, metabolic activity and osteogenic differentiation potential of human bone marrow stromal cells are assessed using fluorescence microscopy and MTS assays. Moreover, tissue non-specific alkaline phosphatase activity served as an early osteoblast production marker. Compared to untextured specimens, both types of textures exhibited higher metabolic activity and cell proliferation. Single-scale ns-DLIP textures encouraged cell extensions anchored in groove regions, while ps-DLIP textures with hierarchical structures promoted cell extensions attaching to nanostructures on sidewalls. The groove width and nanotopographies in groove areas facilitated cell spreading. Surface topography, roughness, and surface chemistry (surface energy, wettability) influenced cell adhesion, proliferation, and differentiation. A comprehensive evaluation of DLIP-generated surface textures, including their topography and chemical states, complements the factors affecting in vitro cell behavior. Overall, this research demonstrates the potential of surface-functionalized 3Dprinted titanium for a novel generation of biocompatible implants. 相似文献
88.
89.
Salowa Methnani Jean-Paul Gauthier Frederic Lafont 《International journal of control》2013,86(4):822-833
In this article, we propose a general methodology for identifying and reconstructing sensor faults on dynamical processes. This methodology is issued from the general identification theory developed in the previous papers (Busvelle, E., and Gauthier, J.-P. (2003), ‘On Determining Unknown Functions in Differential Systems, with an Application to Biological Reactor’, ESAIM: Control, Optimisation and Calculus of Variations, 9, 509–553; Busvelle, E., and Gauthier, J.-P. (2004), ‘New Results on Identifiability of Nonlinear Systems’, in 2nd Symposium on Systems, Structure and Control, Oaxaca, Mexico; Busvelle, E., and Gauthier, J.-P. (2005), ‘Observation and Identification Tools for Non Linear Systems. Application to a Fluid Catalytic Cracker’, International Journal of Control, 78, 208–234): in fact, this identification theory also provides a general framework for the problem of ‘observability with unknown inputs’. Indeed, many problems of fault detection can be formulated as such observability problems, the (eventually additive) faults being just considered as unknown inputs. Our application to ‘sensor fault detection’ for wastewater treatment plants (WWTP) constitutes an ideal academic context to apply the theory: first, in this 3-5 case (3 sensors, 5 states), the theory applies generically and, second, any system is naturally under the ‘observability canonical form’ required to apply the basic high-gain observer from Gauthier and Kupka (Gauthier, J.-P., and Kupka, I. (1994), ‘Observability and Observers for Nonlinear Systems’, SIAM Journal on Control, 32, 975–994). A simulation study on the Bleesbrük WWTP is proposed to show the effectiveness of this approach. 相似文献
90.
Sukhvinder Singh Frederic DrossNiels E. Posthuma Robert Mertens 《Solar Energy Materials & Solar Cells》2011,95(4):1151-1156
A record efficiency of 15.8% (independently confirmed at Fraunhofer ISE calibration laboratory) is reported on large area (120 cm2) n-type mc-Si rear junction Si solar cell. Minor modifications to the industrial process for p-type, such as optimization of Al-alloyed screen-printed emitter and phosphorus front surface field, led to an improvement in cell properties. Large improvement in short-circuit current of the cell was possible by decreasing the cell thickness to 130 μm. 相似文献