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21.
Yong Pan Chen Zhu Wen Bin Zeng Pei Fu Chi Chen Bao Ming Xu Zhong Feng Gao 《Chembiochem : a European journal of chemical biology》2021,22(24):3431-3436
Rapid and sensitive point-of-care testing (POCT) is an extremely critical mission in practical applications, especially for rigorous military medicine, home health care, and in the third world. Here, we report a visual POCT method for adenosine triphosphate (ATP) detection based on Taylor rising in the corner of quadratic geometries between two rod surfaces. We discuss the principle of Taylor rising, demonstrating that it is significantly influenced by contact angle, surface tension, and density of the sample, which are controlled by ATP-dependent rolling circle amplification (RCA). In the presence of ATP, RCA reaction effectively suppresses Taylor-rising behavior, due to the increased contact angle, density, and decreased surface tension. Without addition of ATP, untriggered RCA reaction is favorable for Taylor rising, resulting in a significant height. With this proposed method, visual sensitive detection of ATP without the aid of other instruments is realized with only a 5 μL droplet, which has good selectivity and a low detection limit (17 nM). Importantly, this visual method provides a promising POCT tool for user-friendly molecular diagnostics. 相似文献
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热轧态中锰TRIP钢首先经650 ℃退火2 h,随后在550 ℃进行等温时效热处理,采用场发射扫描电镜(FE-SEM)研究该钢中P的偏聚和时效析出行为的变化情况。结果表明,中锰TRIP钢中P在晶界的偏聚是一种非平衡偏聚现象,临界时间约为50 h,与理论计算结果48 h较为吻合。在局部偏聚区域内,C与P存在共偏聚的关系,即P偏聚量高的地方,C含量也高。而合金元素Nb具有抑制P偏聚的效果,在20~70 h时效时间内,可以相对降低6.57%~19.5%的最大P偏聚量。根据电子背散射衍射(EBSD)菊池线分析,P偏聚量低于2.28at%时,P为固溶状态,高于2.28at.%时,P为析出状态。 相似文献
25.
Ningning Fu Jiaxing Li Ming Wang Lili Ren Youqing Luo 《International journal of molecular sciences》2020,21(22)
An obligate mutualistic relationship exists between the fungus Amylostereum areolatum and woodwasp Sirex noctilio. The fungus digests lignin in the host pine, providing essential nutrients for the growing woodwasp larvae. However, the functional properties of this symbiosis are poorly described. In this study, we identified, cloned, and characterized 14 laccase genes from A. areolatum. These genes encoded proteins of 508 to 529 amino acids and contained three typical copper-oxidase domains, necessary to confer laccase activity. Besides, we performed molecular docking and dynamics simulation of the laccase proteins in complex with lignin compounds (monomers, dimers, trimers, and tetramers). AaLac2, AaLac3, AaLac6, AaLac8, and AaLac10 were found that had low binding energies with all lignin model compounds tested and three of them could maintain stability when binding to these compounds. Among these complexes, amino acid residues ALA, GLN, LEU, PHE, PRO, and SER were commonly present. Our study reveals the molecular basis of A. areolatum laccases interacting with lignin, which is essential for understanding how the fungus provides nutrients to S. noctilio. These findings might also provide guidance for the control of S. noctilio by informing the design of enzyme mutants that could reduce the efficiency of lignin degradation. 相似文献
26.
Single image super resolution (SISR) is an important research content in the
field of computer vision and image processing. With the rapid development of deep
neural networks, different image super-resolution models have emerged. Compared to
some traditional SISR methods, deep learning-based methods can complete the superresolution tasks through a single image. In addition, compared with the SISR methods
using traditional convolutional neural networks, SISR based on generative adversarial
networks (GAN) has achieved the most advanced visual performance. In this review, we
first explore the challenges faced by SISR and introduce some common datasets and
evaluation metrics. Then, we review the improved network structures and loss functions
of GAN-based perceptual SISR. Subsequently, the advantages and disadvantages of
different networks are analyzed by multiple comparative experiments. Finally, we
summarize the paper and look forward to the future development trends of GAN-based
perceptual SISR. 相似文献
27.
This study was undertaken to develop a modified atmosphere package to control microbial growth in ready‐to‐eat (RTE) products stored at ambient temperature. Ethanol and/or limonene associated with modified atmosphere (CO2 : O2 : N2 = 30% : 5% : 65%) was used to inhibit the growth of total air‐borne microorganisms and Escherichia coli in RTE products stored at 25°C. The results indicated that 0.05% ethanol vapour in the headspace was effective to inhibit the growth of air‐borne microorganisms and E. coli at 25°C for 72 h in a model study, and the effectiveness was related to ethanol content. Both 73 ppm limonene and 0.05% ethanol vapour enhanced the bacteriostatic effect of modified atmosphere in RTE sushi roll products, and no off‐flavour was detected using this formulated gas; however, no significant inhibitory effect was observed for RTE cold noodle products. This study concludes that combinations of carbon dioxide, ethanol or limonene vapours are effective to inhibit microbial growth in RTE food at ambient temperature, and the outcome may be due to the hurdle effect. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
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在充分调研阵列声波测井分波提取方法的基础上,针对阿特拉斯公司的交叉多极子阵列声波测井(XMACⅡ)提供的单极波形信息,研究纵波时差提取及高分辨率处理方法。首先利用相关-互功谱法高精度地提取纵波时差,然后针对XMACⅡ仪器的接收阵列,研究组合子阵列的方法,形成共发射子阵列和共接收子阵列,最后利用子阵列对提取的纵波时差做高分辨率处理。处理现场实际测井资料,并将高分辨率处理成果与引进的eXpress软件处理成果、实测的国产高分辨率声波(纵波)测井曲线及岩心纵波时差对比分析,表明该方法提取的纵波时差曲线的纵向分辨率有很大提高,时差计算准确可靠,提取的单极纵波时差与国产高分辨率声波时差及岩心纵波时差的平均相对误差均小于7%。该方法具有较高的实用价值和广阔的应用前景。 相似文献
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