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71.
Nakamoto Y Sakata M Sumiya H Shimizu K Irifune T Matsuoka T Ohishi Y 《The Review of scientific instruments》2011,82(6):066104
Nano-polycrystalline diamonds (NPDs) consist of nanosized diamond grains oriented in random directions. They have high toughness and isotropic mechanical properties. A NPD has neither the cleavage feature nor the anisotropy of hardness peculiar to single-crystal diamonds. Therefore, it is thought to be useful as a diamond anvil. We previously reported the usefulness of a NPD as an anvil for high-pressure development. In this study, some additional high-pressure generating tests using diamond anvils of various shapes prepared from NPDs were conducted to investigate the advantage of using NPDs for anvil applications. The results revealed that the achievable pressure value of a NPD anvil with a culet size of more than 300 μm is about 1.5 to 2 times higher than that of single-crystal diamond anvils, indicating that NPD anvils have considerable potential for large-volume diamond anvils with large culet sizes. 相似文献
72.
Mai Morozumi Hirohisa Izumi Takashi Shimizu Yasuhiro Takeda 《Journal of dairy science》2021,104(6):6463-6471
Extracellular vesicles (EV) are important for delivering biologically active substances to facilitate cell-to-cell communication. Milk-derived EV are widely known because of their potential for immune enhancement. However, procedures for isolating milk-derived EV have not been fully established. To obtain pure milk-derived EV and accurately reveal their function, such procedures must be established. The aim of the present study was to compare methods using commercially available kits for isolating milk-derived EV. Initially, we investigated procedures to remove casein, which is the major obstacle in determining milk-derived EV purity. We separated whey using centrifugation only, acetic acid precipitation, and EDTA precipitation. Then, we isolated milk-derived EV by ultracentrifugation, membrane affinity column, size exclusion chromatography (SEC), polymer-based isolation, or phosphatidylserine-affinity isolation. Using EV count per milligram of protein, which is a good indicator of purity, we determined that acetic acid precipitation was the best method for removing casein. Using nanoparticle tracking analysis, protein quantity analysis, and RNA quantity analysis, we comprehensively compared each isolation method for its purity and yield. We found that SEC-based qEV column (Izon Science) could collect purer milk-derived EV at higher quantities. Thus, a combination of acetic acid precipitation and qEV can effectively isolate high amounts of pure extracellular vesicles from bovine milk. 相似文献
73.
74.
F. Yuasa E. de Doncker N. Hamaguchi T. Ishikawa K. Kato Y. Kurihara J. Fujimoto Y. Shimizu 《Computer Physics Communications》2012,183(10):2136-2144
A new approach is presented to evaluate multi-loop integrals, which appear in the calculation of cross-sections in high-energy physics. It relies on a fully numerical method and is applicable to a wide class of integrals with various mass configurations. As an example, the computation of two-loop planar and non-planar box diagrams is shown. The results are confirmed by comparisons with other techniques, including the reduction method, and by a consistency check using the dispersion relation. 相似文献
75.
Adhesion between polymer surface modified by graft polymerization and tissue during surgery using an ultrasonically activated scalpel device
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Kwangwoo Nam Takuya Iwata Tsuyoshi Kimura Hiroki Ikake Shigeru Shimizu Toru Masuzawa Akio Kishida 《应用聚合物科学杂志》2014,131(20)
In the field of surgery, achieving adhesion between a polymer implant and tissue poses a challenge considering that suturing is not appropriate for the stability of such implants. An ultrasonically activated scalpel that generates heat by mechanical vibration and promotes adhesion between a polymer implant and native tissue by pressing the two materials together has very good potential for application in the field. To determine the type of polymer that is suitable for the purpose, we investigated polyethylene (PE) and polystyrene (PS) films, the surfaces of which were activated by corona discharge. Graft polymerization was then performed on the corona‐treated surfaces to vary their properties. The corona‐treated PE and PS films grafted with poly(acrylic acid), poly(methacrylic acid), poly(vinyl benzylacrylic acid), and poly(hydroxylethyl acrylate), respectively, adhered to the tissue when the ultrasonically activated scalpel was applied. The heat generated by the mechanical vibration and the applied pressure enabled the carboxyl or hydroxyl groups to bond with the proteins in the extracellular matrix. We therefore concluded that it was possible to integrate this technique in the development of new types of polymer devices that could be stably implanted in a living body. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40885. 相似文献
76.
Kenji Imai Koji Takai Tatsunori Hanai Makoto Shiraki Yusuke Suzuki Hideki Hayashi Takafumi Naiki Youichi Nishigaki Eiichi Tomita Masahito Shimizu Hisataka Moriwaki 《International journal of molecular sciences》2014,15(7):11294-11306
Obesity-related metabolic abnormalities, including adipokine imbalance and chronic inflammation, are involved in liver carcinogenesis. Chemerin, a novel adipokine, plays a critical role in adipogenesis, energy metabolism, and inflammation. We evaluated the impact of serum chemerin levels on liver functional reserves in hepatocellular carcinoma (HCC) patients and on the recurrence and prognosis of HCC. This study included 44 patients with any stage of HCC who underwent curative treatment at Gifu Municipal Hospital (Gifu, Japan) between 2006 and 2007. Recurrence-free survival and overall survival were estimated using the Kaplan-Meier method. Serum albumin levels (Pearson’s correlation coefficient; r = 0.3110, p = 0.0399), platelet counts (r = 0.4159, p = 0.0050), and prothrombin times (r = 0.3775, p = 0.0115) were significantly correlated with serum chemerin levels in patients with HCC, and they were inversely correlated with Child-Pugh scores (r = −0.3732, p = 0.0126), serum alanine aminotransferase levels (r = −0.3864, p = 0.0105), and total bilirubin levels (r = −0.4023, p = 0.0068). Among these variables, a multiple comparison test identified that platelet counts and total bilirubin levels were associated with serum chemerin levels (p < 0.0083). No significant correlation was found between serum chemerin levels and recurrence-free survival (p = 0.3691) or overall survival (p = 0.7916). In HCC patients, serum chemerin concentrations were correlated with liver functional reserves and platelet counts, but not with recurrence or prognosis. 相似文献
77.
78.
Kazuyuki Ishikawa Naohiro Kameta Masaru Aoyagi Masumi Asakawa Toshimi Shimizu 《Advanced functional materials》2013,23(13):1606-1606
79.
Kazuyuki Ishikawa Naohiro Kameta Mitsutoshi Masuda Masumi Asakawa Toshimi Shimizu 《Advanced functional materials》2014,24(5):603-609
Boroxines, (R‐BO)3, which can be easily synthesized via a dehydration reaction of boronic acids, R–B(OH)2, selectively self‐assemble in toluene into nanofibers, nanorods, nanotapes, and nanotubes, depending on the aromatic substituent (R). Spectroscopic measurements show that the nanotube consists of a J‐aggregate of the boroxine. Humidification converts the morphology from the nanotube to a sheet as a result of the hydrolysis of the boroxine components and subsequent molecular‐packing rearrangement from the J‐aggregate to an H‐aggregate. Such a transformation leads to the compulsive release of guest molecules encapsulated in the hollow cylinder of the nanotube. The hydrolysis and the molecular‐packing rearrangement described above are suppressed by coordination of pyridine to the boron atom, with the resulting moiety acting as a Lewis acid of the boroxine component. The pyridine‐coordinated nanotube is transformed into a helical coil by humidification. Guest release during the nanotube‐to‐helical‐coil transformation is much slower than during the nanotube‐to‐sheet transformation, but faster than from a nanotube that did not undergo morphological transformation. The storage and release of guest molecules from the boroxine nanotubes can be precisely controlled by adjusting the moisture level and the concentration of Lewis bases, such as amines. 相似文献
80.
Midori Takasaki Kentaro Hara Yutaka Ohkoshi Takayuki Fujii Hiroyasu Shimizu Masaharu Saito 《Polymer Engineering and Science》2014,54(11):2605-2609
Thermoplastic polyurethane fiber webs were prepared using a laser‐heated electrospinning process combined with air blowing. The effect of spinning conditions such as air flow rate and air temperature on fiber diameter and molecular weight was investigated. Although the average fiber diameter decreased with increased air flow rate at each air temperature, the diameter increased when the air flow rate was >15 NL min?1. In addition, the fiber was comparatively thicker with an increase in the air temperature. The variation in the fiber diameter tends to increase with the air flow rate, and a reduction in the molecular weight of the fiber by thermal degradation was suppressed. The thinnest and most uniform fiber with a diameter of 0.9 µm and a diameter coefficient variation of 15% was obtained at an air temperature of 25°C under an air flow rate of 15 NL min?1. This fiber also had a minimum of decreased molecular weight. POLYM. ENG. SCI., 54:2605–2609, 2014. © 2013 Society of Plastics Engineers 相似文献