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31.
Morris Richard W.; Furlong Teri M.; Westbrook R. Frederick 《Canadian Metallurgical Quarterly》2005,31(1):40
Rats were shocked in a context and then exposed to that context in the absence of shock. Shorter intervals between these extinction trials produced more long-term freezing than did longer ones, and shorter intervals between the final extinction trial and test produced more freezing than did longer ones. A short interval between a context extinction trial and test with an extinguished conditioned stimulus (CS) produced more freezing than did a longer one, and a short interval between a nonreinforced context exposure and an extinguished CS reinstated freezing when the CS was tested 24 hr later. The results suggest that recent fear acts to favor subsequent retrieval of the memory formed at conditioning rather than extinction and to render the retrieved memory more salient. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
32.
Identification of genes regulated by interleukin-1beta in human endometrial stromal cells 总被引:4,自引:0,他引:4
Rossi M Sharkey AM Viganò P Fiore G Furlong R Florio P Ambrosini G Smith SK Petraglia F 《Reproduction (Cambridge, England)》2005,130(5):721-729
Interleukin-1beta (IL-1b) is an important immune regulatory factor that in human endometrium plays a role in both menstruation and implantation in the event of pregnancy. It promotes inflammatory-like processes and also stimulates tissue remodelling. We present a cDNA microarray study documenting the major effects of IL-1beta on gene expression in stromal cells from human endometrium. Endometrial stromal cells from five normal healthy women at the mid secretory phase were cultured with or without IL-1beta at 50 and 500 pg/ml for 48 h. cDNA microarrays were used to compare the levels of gene expression in total RNA isolated from cells stimulated with IL-1beta. These cDNA arrays were produced containing 15 164 sequence-verified clones, which included genes known to be important in angiogenesis, immune modulators, apoptosis, cell signalling, extra-cellular matrix (ECM) remodelling and cell cycle regulation. Genes which were regulated by IL-1beta were identified by analysis of the microarray data using the Significance Analysis of Microarrays software package. Upregulated (n = 23) and downregulated (n = 6) different genes were observed, which changed at least 3-fold, at a false discovery rate of less than 2% (P < 0.02). Our results have identified genes regulated by IL-1beta, which are involved in leukocyte recruitment, ECM remodelling and other cellular functions. Changes in three genes, IL-8, colony-stimulating factor 2 and aldoketo reductase family 1 member 1, which were upregulated by IL-1beta, were verified using real-time PCR. Novel functions regulated by IL-1beta in endometrium, including genes involved in free radical protection, and fatty acid metabolism were also identified. These results also provide new insights into the role of IL-1beta in disorders of the endometrium, especially in implantation-related infertility and endometriosis, in which this cytokine plays a major role. 相似文献
33.
Furlong ET Burkhardt MR Gates PM Werner SL Battaglin WA 《The Science of the total environment》2000,248(2-3):135-146
Sulfonylurea (SU), imidazolinone (IMI), and sulfonamide (SA) herbicides are new classes of low-application-rate herbicides increasingly used by farmers. Some of these herbicides affect both weed and crop species at low dosages and must be carefully used. Less is known about the effect of these compounds on non-crop plant species, but a concentration of 100 ng/l in water has been proposed as the threshold for possible plant toxicity for most of these herbicides. Hence, analytical methods must be capable of detecting SUs, IMIs, and SAs at concentrations less than 100 ng/l in ambient water samples. The authors developed a two-cartridge, solid-phase extraction method for isolating 12 SU, 3 IMI, and 1 SA herbicides by using high-performance liquid chromatography/electrospray ionization-mass spectrometry (HPLC/ESI-MS) to identify and quantify these herbicides to 10 ng/l. This method was used to analyze 196 surface- and ground-water samples collected from May to August 1998 throughout the Midwestern United States, and more than 100 quality-assurance and quality-control samples. During the 16 weeks of the study, the HPLC/ESI-MS maintained excellent calibration linearity across the calibration range from 5 to 500 ng/l, with correlation coefficients of 0.9975 or greater. Continuing calibration verification standards at 100-ng/l concentration were analyzed throughout the study, and the average measured concentrations for individual herbicides ranged from 93 to 100 ng/l. Recovery of herbicides from 27 reagent-water samples spiked at 50 and 100 ng/l ranged from 39 to 92%, and averaged 73%. The standard deviation of recoveries ranged from 14 to 26%, and averaged 20%. This variability reflects multiple instruments, operators, and the use of automated and manual sample preparation. Spiked environmental water samples had similar recoveries, although for some herbicides, the sample matrix enhanced recoveries by as much as 200% greater than the spiked concentration. This matrix enhancement was sample- and compound-dependent. Concentrations of herbicides in unspiked duplicate environmental samples were typically within 25% of each other. The results demonstrate the usefulness of HPLC/ESI-MS for determining low-application-rate herbicides at ambient concentrations. 相似文献
34.
35.
A bacterial identification method in which multiple enzyme activities are measured simultaneously and in vivo with electrospray ionization-mass spectrometry (ESI-MS) is described. Whole-cell bacteria are immobilized onto a filter support and incubated with a mixture of substrates. Each substrate is chosen to measure a specific enzyme activity of a targeted bacterium and to produce a tag of unique molecular weight. After a predetermined incubation time, the solution is filtered, and the supernatant consisting of a mixture of released tags and unhydrolyzed substrates is directly analyzed, without chromatographic separation, by ESI-MS. Bacteria remain viable on the filter for further analyses. The method was tested by measuring the aminopeptidase activity of the bacteria Escherichia coli, Bacillus subtilis, Bacillus cereus, and Pseudomonas aeruginosa. The resulting aminopeptidase enzyme profiles allowed the differentiation between the four bacteria tested. The method is rapid, since a multiplex advantage is realized when assaying for multiple enzymes, and it is amenable to automation via a flow injection analysis setup. 相似文献
36.
Lynnworth L. C. Cohen R. Rose J. L. Kim J. O. Furlong E. R. 《IEEE sensors journal》2006,6(6):1488-1496
This paper was motivated by the possibility of extracting from a vortex-shedding strut, in addition to flow velocity V, information on fluid density rho or temperature T, and combining them to obtain mass flowrate. Shedder shapes were diamond and bluff polygon. These shapes are compared as vortex shedders in flowing air or water. V is obtained from the shedding frequency f. In water, V ranged from 0.5 to 4 m/s and, in air, from 0.3 to 15 m/s. Clamp-on ultrasonic transducers generated and, on the diagonally opposite side of the pipe, received the beam that obliquely traversed the wake of the shedder. A continuous-wave transmission across the fluid was modulated by vortices passing through the beam. The modulation frequency yielded f. In air, the bluff polygon yielded f over a 50 : 1 flow range, which was better than the diamond's flow range of 20 : 1. Whether the shedder was a diamond or a bluff polygon, and the fluid air or water, f correlated approximately linearly with the flow velocity V. Using one path of an ultrasonic tag clamp-on flowmeter system, the measured vortex-shedding frequencies were found to be in reasonable agreement with computational-fluid-dynamic predictions for diamond and for bluff-polygon struts. Collectively, the pipe Reynolds number (Re) range was 1000-200 000. With both shedders, operation was demonstrated in laminar- and turbulent-flow regimes. In water flow tests, rotating the diamond (aspect ratio=3) through 90deg about its axis, from broadside to airfoil, diminished the Strouhal number by 17%. When the diamond shedder was tested as a torsional density sensor in flowing air or water, no torsional transit time effect of V was observed, confirming for the first time a 1989 prediction. The negative result in flowing water implies that there were no attached bubbles or microbubbles 相似文献
37.
CE Furlong WF Li LG Costa RJ Richter DM Shih AJ Lusis 《Canadian Metallurgical Quarterly》1998,19(4-5):645-650
Thin-layer chromatographic (TLC)-UV densitometric and gas-chromatographic-mass spectrometric detection (GC-MSD) methods were developed for simultaneous quantification of morphine and codeine in poppy capsules (Papaver somniferum). Morphine and codeine were isolated by extraction with chloroform: isopropanol (3:1, v/v) at pH = 8.5 and by solid-phase extraction on Snap-Cap cartridges at pH = 8.5. The TLC-UV densitometric quantification was performed by external standard method on silica gel plates using ethyl acetate: toluene: methanol: ammonia (68:17:10:5, v/v) as developing solvent and UV detection at 275 nm. For the GC-MSD analysis, the drugs were derivatized with acetic anhydride: pyridine (1:1, v/v) and separated on a 30 m HP5 capillary column. The quantification was performed using nalorphine as internal standard. 相似文献
38.
A national reconnaissance of pharmaceuticals and other organic wastewater contaminants in the United States--I) groundwater 总被引:4,自引:0,他引:4
Barnes KK Kolpin DW Furlong ET Zaugg SD Meyer MT Barber LB 《The Science of the total environment》2008,402(2-3):192-200
As part of the continuing effort to collect baseline information on the environmental occurrence of pharmaceuticals, and other organic wastewater contaminants (OWCs) in the Nation's water resources, water samples were collected from a network of 47 groundwater sites across 18 states in 2000. All samples collected were analyzed for 65 OWCs representing a wide variety of uses and origins. Site selection focused on areas suspected to be susceptible to contamination from either animal or human wastewaters (i.e. down gradient of a landfill, unsewered residential development, or animal feedlot). Thus, sites sampled were not necessarily used as a source of drinking water but provide a variety of geohydrologic environments with potential sources of OWCs. OWCs were detected in 81% of the sites sampled, with 35 of the 65 OWCs being found at least once. The most frequently detected compounds include N,N-diethyltoluamide (35%, insect repellant), bisphenol A (30%, plasticizer), tri(2-chloroethyl) phosphate (30%, fire retardant), sulfamethoxazole (23%, veterinary and human antibiotic), and 4-octylphenol monoethoxylate (19%, detergent metabolite). Although sampling procedures were intended to ensure that all groundwater samples analyzed were indicative of aquifer conditions it is possible that detections of some OWCs could have resulted from leaching of well-construction materials and/or other site-specific conditions related to well construction and materials. Future research will be needed to identify those factors that are most important in determining the occurrence and concentrations of OWCs in groundwater. 相似文献
39.
The friction of a copper surface, which is exposed to diethyl disulfide (DEDS), dimethyl disulfide (DMDS) or dimethyl trisulfide (DMTS) while rubbing by a tungsten carbide pin, are explored in an ultrahigh vacuum tribometer to investigate the effect of the sulfur-to-carbon stoichiometry on gas-phase lubrication. Surface analyses by Auger spectroscopy of the wear scars after rubbing reveal that the amount of sulfur increases with sulfur content of the gas-phase lubricant, as anticipated. It is found that the friction reduction depends on the normal load, where the friction coefficient tends to decrease more at lower loads, and that the load at which the most friction-reducing tribofilm is formed depends on the stoichiometry of the gas-phase lubricant. DEDS (with a sulfur to carbon ratio of 0.5) only reduces the friction coefficient to a minimum value of ~0.28 at a normal load of 0.25 N, for DMDS (S/C = 1.0) friction is reduced to ~0.28 for loads below ~0.7 N, and DMTS (S/C = 1.5) reduces friction to this value for all loads tested. 相似文献
40.
Pamela I. Otto Simone E.F. Guimarães Lucas L. Verardo Ana Luísa S. Azevedo Jeremie Vandenplas Aline C.C. Soares Claudia A. Sevillano Renata Veroneze Maria de Fatima A. Pires Célio de Freitas Márcia Cristina A. Prata John Furlong Rui S. Verneque Marta Fonseca Martins João Cláudio C. Panetto Wanessa A. Carvalho Diego O.R. Gobo Marcos Vinícius G.B. da Silva Marco A. Machado 《Journal of dairy science》2018,101(12):11020-11032