Power generation of microbial fuel cells (MFCs) with low cathodic platinum loading

This study aims at investigating the effects of platinum (Pt) loadings on the cathodic reactions in Single Chamber Microbial Fuel Cells (SCMFCs) and developing cost-effective MFC operational protocols. The power generation of SCMFCs was examined with different Pt loadings (0.005–1 mgPt/cm²) on cathodes. The results showed that the power generation of the SCMFCs with 0.5–1 mgPt/cm² were the highest in the tests, decreased 10–15% at 0.01–0.25 mgPt/cm², and decreased further 10–15% at 0.005 mgPt/cm². The SCMFCs with Pt-free cathode (graphite) had the lowest power generation. In addition, the power generation of SCMFCs with different Pt loadings were compared in raw wastewater (Chemical oxygen demand (COD): 0.36 g/L) and wastewater enriched with sodium acetate (COD: 2.95 g/L). The solution conductivity in SCMFCs decreased with the degradation of organic substrates. Daily polarization curves (V–I) showed a decrease in current generation and an increase in ohmic losses over the operational period (8 days). The SCMFCs (with 0.5–1 mgPt/cm² at cathode) fed with wastewater and sodium acetate (NaOAc) reached the highest power generation (786 mW/m²), while the SCMFCs (with 0.5–1 mgPt/cm² at cathode) fed only with wastewater obtained the lower power generation (81 mW/m²). The study demonstrated that lowering the Pt loadings in two magnitude orders (1 to 0.01, 0.5 to 0.005 mgPt/cm²) only reduced the power generation of 15–30%, and this reduction of the power generation become less substantial with the decrease in the solution conductivity of SCMFCs.     

Chemical hydrogels based on a hyaluronic acid-graft-α-elastin derivative as potential scaffolds for tissue engineering

In this work hyaluronic acid (HA) functionalized with ethylenediamine (EDA) has been employed to graft α-elastin. In particular a HA-EDA derivative bearing 50 mol% of pendant amino groups has been successfully employed to produce the copolymer HA-EDA-g-α-elastin containing 32% w/w of protein. After grafting with α-elastin, remaining free amino groups reacted with ethylene glycol diglycidyl ether (EGDGE) for producing chemical hydrogels, proposed as scaffolds for tissue engineering. Swelling degree, resistance to chemical and enzymatic hydrolysis, as well as preliminary biological properties of HA-EDA-g-α-elastin/EGDGE scaffold have been evaluated and compared with a HA-EDA/EGDGE scaffold. The presence of α-elastin grafted to HA-EDA improves attachment, viability and proliferation of primary rat dermal fibroblasts and human umbilical artery smooth muscle cells. Biological performance of HA-EDA-g-α-elastin/EGDGE scaffold resulted comparable to that of a commercial collagen type I sponge (Antema®), chosen as a positive control.     

The role played by the alpha-helix in the unfolding pathway and stability of azurin: switching between hierarchic and nonhierarchic folding

The role played by the alpha-helix in determining the structure, the stability and the unfolding mechanism of azurin was addressed by studying a helix-depleted azurin variant produced by site-directed mutagenesis. The protein structure was investigated by CD, 1D (1)H NMR, fluorescence spectroscopy measurements and MD simulations, whilst EPR, UV-visible and cyclic voltammetry experiments were carried out to investigate the geometry and the properties of the Cu(II) site. The effects of the alpha-helix depletion on the thermal stability and the unfolding pathway of the protein were determined by DSC, UV/visible and fluorescence measurements at increasing temperature. The results show that, in the absence of the alpha-helix segment, the overall protein structure is maintained, and that only the Cu site is slightly modified. In contrast, the protein stability is diminished by about 60% with respect to the wild-type azurin. Moreover, the unfolding pathway of the mutant azurin involves the presence of detectable intermediates. In comparison with previous studies concerning other small beta-sheet cupredoxins, the results as a whole support the hypothesis that the presence of the alpha-helix can switch the folding of azurin from a hierarchic to a nonhierarchic mechanism in which the highly conserved beta-sheet core provides a scaffold for cooperative folding of the wild-type protein.     

Evolution and control of oxygen order in a cuprate superconductor

The disposition of defects in metal oxides is a key attribute exploited for applications from fuel cells and catalysts to superconducting devices and memristors. The most typical defects are mobile excess oxygens and oxygen vacancies, which can be manipulated by a variety of thermal protocols as well as optical and d.c. electric fields. Here we report the X-ray writing of high-quality superconducting regions, derived from defect ordering, in the superoxygenated layered cuprate, La?CuO(4+y). Irradiation of a poor superconductor prepared by rapid thermal quenching results first in the growth of ordered regions, with an enhancement of superconductivity becoming visible only after a waiting time, as is characteristic of other systems such as ferroelectrics, where strain must be accommodated for order to become extended. However, in La?CuO(4+y), we are able to resolve all aspects of the growth of (oxygen) intercalant order, including an extraordinary excursion from low to high and back to low anisotropy of the ordered regions. We can also clearly associate the onset of high-quality superconductivity with defect ordering in two dimensions. Additional experiments with small beams demonstrate a photoresist-free, single-step strategy for writing functional materials.     

Palladium(II)/Copper Halide/Solvent Combination for Selective Intramolecular Domino Reactions of Indolecarboxylic Acid Allylamides: An Unprecedented Arylation/Esterification Sequence

Intramolecular oxidative palladium‐catalyzed reactions of indolylallylamides in the presence of the couple bis(acetonitrile) palladium dichloride and copper(II) halide are described. Starting from 2‐ and 3‐indolylallylamides and involving in both cases the C‐3 position of the indole nucleus, variously substituted β‐carbolinones were obtained by arylation/halogenation, arylation/esterification or arylation/carboalkoxylation processes. On the other hand, an unusual aminohalogenation/halogenation sequence performed on 2‐indolylallylamides gave rise to pyrazino[1,2‐a]indole products. The carboesterification process is the result of an unknown path that involves the DMF or DMA used as solvent. The outcome of the reactions of the 3‐indolylallylamides arises from a totally selective 1,2‐migration of the acyl group on the supposed spiro intermediates formed from the nucleophilic attack of the C‐3 indole position.     

Patient dose in digital projection radiography

In projection radiography, two types of digital imaging systems are currently available, computed radiography (CR) and direct radiography (DR): a difference between them can be stated in terms of dose and image quality. In the Radiology Emergency Department of our hospital, a flat-panel DR equipment (Siemens Axiom Aristos FX) and two CR systems (Kodak CR-850) are employed. In 2006, five standard radiographic examinations (abdomen, chest, lumbar spine, pelvis, skull) were considered: doses delivered to patients in terms of both entrance skin dose (ESD) and effective dose (E) were calculated and compared in order to study the dosimetric discrepancies between CR and DR. Assessment of image quality is undertaken by Consultant Radiologists to ensure that the quality criteria for diagnostic radiographic images of the European guidelines were met. Results showed that both ESD and E in DR are lower than that in CR; all images met the criteria in the European Guidelines for both modalities and were used for reporting by the radiologists. Since the operators are the same and the image quality is comparable in both modalities, this study shows that in the considered examinations, DR can perform better than CR from a dosimetric point of view.     

Unified theory for confinement of RC solid and hollow circular columns

Hollow bridge piers are incorporated in tall bridges to maximize the structural efficiency of the strength-mass and stiffness-mass ratios. Column jacketing with fiber reinforced polymer (FRP) composite materials has been extensively studied in recent years but it is uncertain how FRP jackets may perform when retrofitting hollow concrete columns since the topic has been little researched.A confinement model for circular hollow sections is proposed which can be extended to the case of solid sections. The model is able to estimate confinement effectiveness and to plot stress–strain relationships, which are different in the case of solid and hollow sections.     

A LC-HRMS After QuEChERS Cleanup Method for the Rapid Determination of Dye Residues in Fish Products

The reliability of a rapid LC-HRMS method was studied in order to find a sensitive and accurate, simple, cheap, and rapid method for screening and quantitative determination of malachite green (MG), leucomalachite green (LMG), brilliant green (BG), crystal violet (CV), and leucocrystal violet (LCV) in fish muscle. All the analytes were extracted using a mixture of acetonitrile and citrate buffer, while the cleanup phase was carried out by Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method. All the data analyzed were acquired using both full MS and dd-MS² modes. Good specificity, precision (relative standard deviation <11% for each sample tested), recovery (10–60%), decision limit (CCα between 0.55 and 0.62 μg kg^?1), detection capabilities (CCβ between 0.65 and 0.78 μg kg^?1), and ruggedness were achieved for the reliability of the method. Satisfactory results were obtained during the linearity test in the range of 0.10–2 ng mL^?1 (r ² > 0.999). Best recoveries were obtained by the QuEChERS cleanup method for all the analytes examined, presenting values between 70 and 104%. The application of 70,000 FWHM mass resolution and narrow mass windows significantly improved the selectivity of the method, leading to simultaneous screening and quantification of dye residues in comparison to other methods proposed in literature. The optimized method proposed in this work enables a simple and fast preparation; it offers exceptional sensitivity and selectivity and maximizes efficiency and reproducibility with a low consumption of reagents. Finally, the present method was successfully employed to detect dye residues in 73 fish samples, as provided for the national residue control plan.     

Standard output versus standard gross margin,a new paradigm in the EU farm economic typology: what are the implications for wine-grape growers?

A new farm economic typology has recently been introduced in the EU. This study compares the economic performance of wine-grape growers in four important quality wine areas of Sicily taking into account the different EU economic typology standards of standard gross margin (the old method) and standard output (the new method). The objective of this study is to identify the differences between the two methods and to ascertain the potential implications for small wine-grape growers’ profitability and their access to public support. We seek to determine whether restrictions can be identified that limit the potential benefits from rural development policies.

For this purpose, we applied the economic criteria of the EU to a representative sample of Sicilian wine-grape growers in order to compare the economic profitability of wine-grape farms in protected designation of origin areas. This comparative approach has allowed us to categorize the economic size of the sample in accordance with recent EU modifications and discuss the possible impacts that the adoption of the new economic criteria would have on access to the Rural Development Programme measures for wine-grape growers.     

Derivative FTIR spectroscopy for cluster analysis and classification of morocco olive oils

Fourier transform infrared (FTIR) spectra were employed for differentiation and classification of olive oils from several producing regions of Morocco. A preliminary treatment of the FTIR data was done by a derivative elaboration based on the Savitzky–Golay algorithm to reduce the noise and extract a largest number of analytical information from the spectra. A multivariate statistical procedure based on cluster analysis (CA) coupled to partial least squares-discriminant analysis (PLS-DA), was elaborated, providing an effective classification method. On the basis of a hierarchical agglomerative CA and principal component analysis (PCA), four distinctive clusters were recognised. The PLS-DA procedure was then applied to classify samples from the same regions, picked in different times, or unknown olive oil samples. The model was optimised by applying the Martens’ Uncertainty Test that provided to select the wavelength zones giving the most useful analytical information. The proposed method furnished results reliable in classifying olive oils from different lands with the advantages of being rapid, inexpensive and requiring no prior separation procedure.