面向对象数据库在压裂措施管理系统中的应用

简要分析了面向对象数据库技术产生的必然性,着重介绍一种面向对象数据库技术——对象关系型数据库,包括它的内容和特点;以及基于实际研发项目全面阐述了压裂措施管理系统中面向对象数据库的统一建模,并对其实现数据共享提供基础性的统一数据结构的支持。     

葡聚糖/PDA复合凝胶的制备、表征及其染料吸附性能研究

以葡聚糖和聚多巴胺(PDA)为原料、环氧氯丙烷(EPI)为交联剂制备葡聚糖/PDA复合凝胶(DPE),并将其应用于亚甲基蓝(MB)的吸附。利用场发射扫描电镜(SEM)、红外光谱仪(FTIR)、电子万能试验机等设备对DPE的结构和性能进行表征。探讨了EPI用量对DPE微观孔径和机械性能的影响,利用紫外可见分光光度计(UV-vis)考察了DPE对MB的吸附性能。结果表明：DPE的微观孔径与EPI添加量成反比,机械性能与EPI添加量成正比。EPI对MB的吸附在80 min左右达到吸附平衡,最大吸附量为36 mg·g^-1,其吸附行为更符合准二级吸附动力学模型,说明DPE对MB的吸附主要为化学吸附。     

基于LoRa传输的高精度水温差监测系统

以高炉出铁口冷却壁为研究对象,设计了一种基于远距离无线通信(LoRa)技术的水温差监测系统。该系统采用一种改进式比率电桥电路,其中温度传感器为PT1000,其测量精度能达到0.01℃,并且将低功耗的LoRa技术作为系统的通信方式,通过RS-485总线将温度数据传输给上位机,记录实时的水温差。最后在某钢厂复杂的环境下,验证了本方案能准确监测冷却壁进出口水温差,对后期分析高炉侵蚀状态具有良好的工程前景。     

Function of Graphene Oxide as the “Nanoquencher” for Hg2+ Detection Using an Exonuclease I-Assisted Biosensor

Graphene oxide is well known for its excellent fluorescence quenching ability. In this study, positively charged graphene oxide (pGO25000) was developed as a fluorescence quencher that is water-soluble and synthesized by grafting polyetherimide onto graphene oxide nanosheets by a carbodiimide reaction. Compared to graphene oxide, the fluorescence quenching ability of pGO25000 is significantly improved by the increase in the affinity between pGO25000 and the DNA strand, which is introduced by the additional electrostatic interaction. The FAM-labeled single-stranded DNA probe can be almost completely quenched at concentrations of pGO25000 as low as 0.1 μg/mL. A simple and novel FAM-labeled single-stranded DNA sensor was designed for Hg²⁺ detection to take advantage of exonuclease I-triggered single-stranded DNA hydrolysis, and pGO25000 acted as a fluorescence quencher. The FAM-labeled single-stranded DNA probe is present as a hairpin structure by the formation of T–Hg²⁺–T when Hg²⁺ is present, and no fluorescence is observed. It is digested by exonuclease I without Hg²⁺, and fluorescence is recovered. The fluorescence intensity of the proposed biosensor was positively correlated with the Hg²⁺ concentration in the range of 0–250 nM (R² = 0.9955), with a seasonable limit of detection (3σ) cal. 3.93 nM. It was successfully applied to real samples of pond water for Hg²⁺ detection, obtaining a recovery rate from 99.6% to 101.1%.     

基于耦合分析的热流系统协同设计

作为综合能源系统的一部分, 热流系统通常在稳态假设下设计其物理系统, 在此基础上设计控制器. 这种 方法忽略了系统与控制器设计间的耦合关系, 导致系统暂态性能不佳. 为此, 本文提出一种协同设计方法, 以系统总 质量、熵产率以及控制效果为设计度量, 基于热流系统数学模型及其熵产率模型, 结合嵌套设计方法和同步设计方 法完成系统设计. 考虑到设计过程中设计参数多、变量范围大的问题, 引入时间序列模式距离, 构造相关度度量函 数来分析物理系统与控制器之间的耦合关系, 从而减少非耦合参数. 同时, 利用数据分析方法进一步缩小了待设计 参数范围. 为验证方法的有效性, 以一个理想热流系统为设计实例, 仿真结果表明, 通过相关度度量函数及其数据分 析, 可以将设计参数个数减少66%, 选取范围缩小26%. 相比于传统顺序设计方法, 经过协同设计方法设计的系统控 制误差可减少29%, 且设计控制器抗扰能力也优于频域方法和SIMC方法.     

Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy

Litter size is an important indicator to measure the production capacity of commercial pigs. Spontaneous embryo loss is an essential factor in determining sow litter size. In early pregnancy, spontaneous embryo loss in porcine is as high as 20–30% during embryo implantation. However, the specific molecular mechanism underlying spontaneous embryo loss at the end of embryo implantation remains unknown. Therefore, we comprehensively used small RNA sequencing technology, bioinformatics analysis, and molecular experiments to determine the microRNA (miRNA) expression profile in the healthy and arresting embryo implantation site of porcine endometrium on day of gestation (DG) 28. A total of 464 miRNAs were identified in arresting endometrium (AE) and healthy endometrium (HE), and 139 differentially expressed miRNAs (DEMs) were screened. We combined the mRNA sequencing dataset from the SRA database to predict the target genes of these miRNAs. A quantitative real-time PCR assay identified the expression levels of miRNAs and mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed target genes of DEMs, mainly enriched in epithelial development and amino acids metabolism-related pathways. We performed fluorescence in situ hybridization (FISH) and the dual-luciferase report gene assay to confirm miRNA and predicted target gene binding. miR-205 may inhibit its expression by combining 3′-untranslated regions (3′ UTR) of tubulointerstitial nephritis antigen-like 1 (TINAGL1). The resulting inhibition of angiogenesis in the maternal endometrium ultimately leads to the formation of arresting embryos during the implantation period. This study provides a reference for the effect of miRNA on the successful implantation of pig embryos in early gestation.     

负载氯喹的骨靶向纳米颗粒打破肿瘤细胞增殖与骨吸收之间的恶性循环

肿瘤细胞增殖与骨吸收之间的恶性循环加剧了骨肿瘤的进展和转移风险.为此,我们设计并制备了聚乙二醇-阿仑膦酸钠修饰的聚多巴胺(PPA)纳米粒子,并在其表面负载自噬抑制剂氯喹(CQ),期望利用该治疗载体(PPA/CQ)打破肿瘤细胞增殖与骨吸收之间的恶性循环,从而有效地治疗骨肿瘤.实验证明,PPA/CQ可以有效地富集到骨组织,...     

酚醛树脂弱凝胶驱注入速度的研究

注入速度是弱凝胶驱开发过程中重要的设计参数,选取成胶时间3d的酚醛树脂弱凝胶体系,考察了注入速度对弱凝胶驱油效果的影响.结果表明,随着注入速度增加,对低渗层易造成伤害,注入速度由1 mL/min提高至12 mL/min时,高渗层的分流率由33.7％增加至54.9％,高渗层封堵效果变差,提高采收率下降了7.74％.提出弱凝胶体系注入速度不宜过大.     

基于Moodle和PhoneGap的移动学习客户端的设计与应用研究

本文将结合用户移动学习现状及偏好,确定移动学习客户端的系统需求,进而基于Moodle和PhoneGap平台,实现对移动学习客户端的设计,并将移动学习客户端应用到实际教学过程中,确定应用成效。