一致持久的带外虚拟化系统

存储虚拟化能够全面提升存储区域网络的服务质量,而带外虚拟化与带内虚拟化相比具有性能高和扩展性好等优点．提出了运用按序操作、REDO日志以及日志完整性鉴别,共同保证带外虚拟化跨越系统崩溃或断电事故后仍旧可用;设计了一种基于关系模型的磁盘上虚拟化元数据组织方式,它具有可读性好和容易修改的优点;给出了通过分析磁盘分区表兼容典型遗留存储系统的有效方法,它具有庞大遗留系统零切割时间的特点．基于上述关键技术实现的原型系统,在典型实验中表现了很好的性能．     

高压线路加装避雷器可减少线路雷击跳闸率

减少线路雷击跳闸率已成为人们共同关注的课题。国内外运行经验表明，加装线路避雷器可有效降低线路雷击跳闸率。浙江电力部门针对雷击跳闸频繁的线路，在易击段加装了线路型避雷器，积累了新的线路防雷经验。     

ATM无源光网络中直流耦合突发模式光接收机分析

本文根据异步传输模式(ATM)无源光网络(APON)的实际要求,对直流耦合突发模式光接收机进行了理论分析,结果表明基于动态峰值检测的突发模式接收机其灵敏度代价和传输容量代价过高,而基于局部峰值检测的突发模式接收机则可较好满足APON的要求.     

基于双CPU技术的泵和马达控制系统的研究与设计

为获取泵和马达的静态与动态特性,设计了基于ADμC812和TMS320F206的双CPU结构的多传感器融合与控制系统。介绍了该系统的体系结构、硬件配置、接口电路、工作原理和工作流程。实现了传感器信息融合的高速高精度采集、复杂算法的大数据量实时计算以及模拟与开关量并有的多通道控制等功能,系统硬件结构简单。该系统作为泵、马达综合试验台的子系统,用于泵、马达性能试验。应用表明该系统能够满足试验要求,具有很好的可靠性和实时性。     

Study on destructions and reinforcement measures of mined constructions

Ground movement and deformation caused by underground mining would destroy the constructions located within subsidence area. Constructions would suffer different destructions with different location. The effect of ground deformation caused by underground mining to constructions was analyzed. Analyzed the destructive types of constructions in different ground movement and deformation. Taking a two-storey building for example, the walls were reinforced by computation before mining. To protect the construction the reinforcement measures to the construction before mining were studied.     

白藜芦醇糖苷类衍生物的设计与合成

以白藜芦醇及其类似物紫檀芪为原料,采用Schmidt成苷法、相转移催化法,分别与溴代糖和乙酰氨基糖反应,得紫檀芪乙酰葡萄糖苷和(E)-5,4'-二叔丁基二甲基硅基-3-(2"-乙酰氨基-3",4",6"-三乙酰葡萄糖二苯乙烯,其结构经红外光谱、核磁共振氢谱和质谱表征.     

Generation and Identification of the Number of Copies of Exogenous Genes and the T-DNA Insertion Site in SCN-Resistance Transformation Event ZHs1-2

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) causes an estimated economic loss of about USD 3 billion each year in soybean (Glycine max L.) production worldwide. Overexpression of resistance genes against SCN provides a powerful approach to develop SCN resistance cultivars in soybean. The clarification of molecular characterization in transformation events is a prerequisite for ecological risk assessment, food safety, and commercial release of genetically modified crops. Here, we generated transgenic events harboring the BCN (beet cyst nematode) resistance Hs1^pro−1 gene using the Agrobacterium-mediated method in soybean, evaluated their resistance to SCN infection, and clarified the molecular characterization of one of the transformation events. Five independent and stable inheritable transformation events were generated by an Agrobacterium-mediated transformation method. SCN resistance tests showed the average number of developed females per plant and female index (FI) in T4 ZHs1-1, ZHs1-2, ZHs1-3, ZHs1-4, and ZHs1-5 transformation events were significantly lower than that in the nontransgenic control. Among these, the ZHs1-2 transformation event had the lowest number of developed females per plant and FI. Southern hybridization showed the exogenous target Hs1^pro−1 gene was inserted in one copy and the Bar gene was inserted two copies in the ZHs1-2 transformation event. The exogenous T-DNA fragment was integrated in the reverse position of Chr02: 5351566–5231578 (mainly the Bar gene expression cassette) and in the forward position of Chr03: 17083358–17083400 (intact T-DNA, including Hs1^pro−1 and Bar gene expression cassette) using a whole genome sequencing method (WGS). The results of WGS method and Southern hybridization were consistent. All the functional elements of exogenous T-DNA fragments were verified by PCR using specific primer pairs in the T5 and T6 ZHs1-2 transformation events. These results demonstrated that the overexpression of Hs1^pro−1 gene enhanced SCN resistance, and provide an important reference for the biosafety assessment and the labeling detection in transformation event ZHs1-2.     

Processing,Quality,Safety,and Acceptance of Meat Analogue Products

Rapid global population growth has caused an increasing need for products containing protein.Meat products are the most common high-protein food source,but impa...     

Development of a Multi-Epitope Vaccine for Mycoplasma hyopneumoniae and Evaluation of Its Immune Responses in Mice and Piglets

Mycoplasma hyopneumoniae (Mhp), the primary pathogen causing Mycoplasma pneumonia of swine (MPS), brings massive economic losses worldwide. Genomic variability and post-translational protein modification can enhance the immune evasion of Mhp, which makes MPS prone to recurrent outbreaks on farms, even with vaccination or other treatments. The reverse vaccinology pipeline has been developed as an attractive potential method for vaccine development due to its high efficiency and applicability. In this study, a multi-epitope vaccine for Mhp was developed, and its immune responses were evaluated in mice and piglets. Genomic core proteins of Mhp were retrieved through pan-genome analysis, and four immunodominant antigens were screened by host homologous protein removal, membrane protein screening, and virulence factor identification. One immunodominant antigen, {"type":"entrez-protein","attrs":{"text":"AAV27984.1","term_id":"53987783","term_text":"AAV27984.1"}}AAV27984.1 (membrane nuclease), was expressed by E. coli and named rMhp597. For epitope prioritization, 35 B-cell-derived epitopes were identified from the four immunodominant antigens, and 10 MHC-I and 6 MHC-II binding epitopes were further identified. The MHC-I/II binding epitopes were merged and combined to produce recombinant proteins MhpMEV and MhpMEVC6His, which were used for animal immunization and structural analysis, respectively. Immunization of mice and piglets demonstrated that MhpMEV could induce humoral and cellular immune responses. The mouse serum antibodies could detect all 11 synthetic epitopes, and the piglet antiserum suppressed the nuclease activity of rMhp597. Moreover, piglet serum antibodies could also detect cultured Mhp strain 168. In summary, this study provides immunoassay results for a multi-epitope vaccine derived from the reverse vaccinology pipeline, and offers an alternative vaccine for MPS.     

Spatial distribution of laser energy and its influence on the stability of extreme narrow keyholes during ultra-high power laser welding

The instability of extremely narrow keyholes is the main challenge in high-power laser welding.In this work,the spatial energy distribution and its influence on the stability of extremely narrow keyholes during ultra-high power laser welding are studied.A multiphase flow model that considers the vapor plume impact and the multiple scattering of laser radiation is built to simulate the topology and downstream wave flow on the keyhole wall.Drastic keyhole fluctuation is due to excessive energy acc...