Lactobacillus fermentum ACA-DC 179 displays probiotic potential in vitro and protects against trinitrobenzene sulfonic acid (TNBS)-induced colitis and Salmonella infection in murine models

Lactobacillus fermentum ACA-DC 179, Lactobacillus plantarum ACA-DC 287 and Streptococcus macedonicus ACA-DC 198 were studied for their probiotic potential. Firstly, strains were screened for antimicrobial activity towards a broad range of target strains, including lactic acid bacteria, food spoilage and pathogenic bacteria. L. fermentum ACA-DC 179 was active against five streptococci, including the two pathogenic strains Streptococcus oralis LMG 14532T and Streptococcus pneumoniae LMG 14545T. S. macedonicus ACA-DC 198 was active against the majority of the strains tested, including not only lactic acid bacteria but also many food spoilage or pathogenic species. The three potential probiotic strains were found to survive variably at pH 2.5 and were unaffected by bile salts. Only S. macedonicus ACA-DC 198 exhibited bile salt hydrolase activity, while none of the strains was haemolytic. Moreover, strains exhibited variable susceptibility towards commonly used antibiotics. L. plantarum ACA-DC 287 and S. macedonicus ACA-DC 198 induced the secretion of the pro-inflammatory cytokines IL-12, IFN-gamma and TNF-alpha by human peripheral blood mononuclear cells. Also elevated levels of the anti-inflammatory IL-10 were observed with L. fermentum ACA-DC 179. This strain consequently was found to significantly reduce colitis in a TNBS-induced colitis mouse model. Furthermore, L. fermentum ACA-DC 179 was successfully applied in an experimental Salmonella-infection mouse model. To conclude, strain L. fermentum ACA-DC 179 possesses desirable probiotic properties, such as antimicrobial activity and immunomodulation in vitro, which were confirmed in vivo by the use of animal models.     

Engineering the xenobiotic substrate specificity of maize glutathione S-transferase I

Glutathione S-transferases (GSTs) are a heterogeneous family of enzymes that catalyse the conjugation of glutathione (GSH) to electrophilic sites on a variety of hydrophobic substrates. In the present study three amino acid residues (Trp12, Phe35 and Ile118) of the xenobiotic binding site (H-site) of maize GST I were altered in order to evaluate their contribution to substrate binding and catalysis. These residues are not conserved and hence may affect substrate specificity and/or product dissociation. The results demonstrate that these residues are important structural moieties that modulate an enzyme's catalytic efficiency and specificity. Phe35 and Ile118 also participate in k(cat) regulation by affecting the rate-limiting step of the catalytic reaction. The effect of temperature on the catalytic activity of the wild-type and mutant enzymes was also investigated. Biphasic Arrhenius and Eyring plots for the wild-type enzyme showed an apparent transition temperature at 35 degrees C, which seems to be the result of a change in the rate-limiting step of the catalytic reaction. Thermodynamic analysis of the activity data showed that the activation energy increases at low temperatures, whereas the entropy change seems to be the main determinant that contributes to the rate-limiting step at high temperatures.     

Occurrence and fate of endocrine disrupters in Greek sewage treatment plants

The occurrence of five endocrine-disrupting compounds (EDCs), namely 4-n-nonylphenol (4-n-NP), nonylphenol monoethoxylate (NP1EO), nonylphenol diethoxylate (NP2EO), triclosan (TCS) and bisphenol A (BPA), was assessed in the raw, treated wastewater and sewage sludge of eight sewage treatment plants (STPs) in Greece. The analytes were extracted by solid-phase extraction (dissolved phase) or sonication (solid phase). Qualitative and quantitative analyses were performed by gas chromatography-mass spectrometry (GC-MS). The average concentrations in the raw and treated wastewater ranged from 0.23 (4-n-NP) to 5.76microgL(-1) (NP1EO) and from 0.15 (BPA) to 1.84microgL(-1) (NP2EO), respectively. A great part of the detected EDCs was sorbed on suspended solids. In sewage sludge, the average concentrations ranged between 0.17 (4-n-NP) and 12.3microgg(-1)dw (NP1EO). Analysis of daily mass flows in STP of Athens showed that, with the exception of 4-n-NP, all other EDCs were significantly removed (>85%) during wastewater treatment. Regarding the fate of these compounds, a significant part ranging from 45% (for TCS) to more than 70% (for NP1EO, NP2EO and BPA) was transformed by abiotic or biotic mechanisms, while the rest was accumulated in sewage sludge or disposed to the environment via the effluents. Calculation of risk quotients showed the existence of possible threat due to the presence of certain EDCs in treated wastewater and sludge.     

Employee perceptions on the implementation of robotic manufacturing technology.

[Correction Notice: An erratum for this article was reported in Vol 71(3) of Journal of Applied Psychology (see record 2008-10743-001). Several crucial horizontal rules were omitted from Table 2. The corrected table appears in the erratum.] A review of the industrial robotics literature identified 4 areas of employee concern during the implementation of robots: general robotics orientation, job security, management concern, and expected changes. A principal factors analysis of a 58-item questionnaire generated to measure these dimensions extracted 4 factors that reproduced the a priori conceptual areas. Composite scales formed from items loading on these factors yielded acceptable reliabilities. A discriminant analysis using the scale scores indicated significant group differences among 316 manufacturing employees in 3 occupational classes—assembly line workers, job setters, and skilled trades. These results, corroborated by a content analysis of an open-ended question, show that low-skill workers reacted negatively toward the implementation of robots, perceiving them largely as threats to their job security. High-skill workers reacted more positively toward the robots and perceived the implementation as providing opportunities to expand their skills. (31 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Perceived versus objective urban opportunities and the migration of Venezuelan youths

A multivariate regression model incorporating both objective economic opportunity and objective quality-of-life measures is able to explain 65% of the variation in young adult in-migration rates for thirty Venezuelan cities in 1971. The objective variables mean annual temperature, level of secondary education, and objective income at the destination are largely responsible for this explanation. A second model, incorporating variables which measure perceived economic opportunity and perceived quality-of-life at the urban destination is then formulated. This perceptual model raises the level of explanation to 83%. Moreover, analysis of the regression coefficients for the objective variables in both models indicates that attractive factors are perceived correctly by migrants while repulsive factors are not.     

Dynamic elastic-plastic response of a containment vessel to fluid pressure pulses

The dynamic analysis of the wall of a fluid-filled unstiffened nuclear containment vessel, to the fluid pressure exerted on it when the relief valve discharge piping is cleared, is extended into the plastic range using two versions of an elastic-plastic shell theory.     

Rule-based query personalization in digital libraries

Searching a digital library is typically a tedious task. A system can improve information access by building on knowledge about a user acquired in a user profile in order to customize information access both in terms of the information returned in response to a query (query personalization) as well as in terms of the presentation of the results (presentation personalization). In this paper, we focus on query personalization in digital libraries; in particular, we address structured queries involving metadata stored in relational databases. We describe the specification of user preferences at the level of a user profile and the process of query personalization with the use of query-rewriting rules.     

Vibrational spectroscopic study on the origin of stress oscillation during step-wise stretching in poly(ethylene terephthalate)

The origin of the phenomenon of stress oscillation during step-wise stretching at room temperature for amorphous poly(ethylene terephthalate) (PET) was investigated using vibrational spectroscopy. For the first time, transmission Fourier transform infrared (FT-IR), attenuated total reflection (ATR) FT-IR, and micro-Raman spectroscopies were used in order to investigate the correlation of the orientation of the molecular chains, their conformational transformations, and the appearance of stress-induced crystallization to the phenomenon of stress oscillation during the step-wise stretching procedure. The phenomenon of stress oscillation occurs when amorphous PET is exposed to mechanical stress during which the extension rate is increased in a step-wise manner. This phenomenon leads to the formation of a pattern of opaque and transparent stripes ('striated' or oscillating region), clearly distinguished from the unstretched ('bulk') and the 'necking' regions. Both infrared and Raman spectroscopic investigations revealed that the main conformational transformations and a significant increase of the crystallinity occur simultaneously in the 'striated' region. Polarized infrared experiments showed the presence of increased molecular orientation, which is more profound for the 'intense striated' region. Finally, micro-Raman spectroscopy allowed the study of opaque and transparent stripes individually and showed that the opaque stripes are more crystalline. Thus, our findings provide conclusive experimental support for the theory, which directly correlates the appearance of the stress-oscillation phenomenon with the induction of crystallinity and heat release and is based on Barenblatt's model. Our study also provides new conformational assignments for the infrared bands in PET for the high-frequency region from 3200 to 3800 cm(-1). Specifically, the bands at 3336 cm(-1) and at 3298 cm(-1) have been attributed to the trans and gauche conformations, respectively.     

The Constant Shape Parameter Assumption in Weibull Regression

ABSTRACT

The usual assumption in Weibull regression is that the scale parameter is a function of the predictor variables, and the shape parameter is constant. We consider the problem of estimating parameters in the presence of a nonconstant shape parameter and the effect of assuming a constant shape parameter when it really is not constant. We consider both classical and Bayesian methods of estimation. The misspecification of a constant shape parameter can lead to a loss of power for tests regarding the slope parameters. We find that prediction intervals can be inaccurate when the shape parameter is incorrectly assumed to be constant.     

Risky and aggressive driving in young adults: Personality matters

Young, novice drivers constitute a disproportionate percentage of fatalities and injuries in road traffic accidents around the world. This study, attempts to identify motivational factors behind risky driving behavior, and examines the role of personality, especially sensation seeking, impulsivity and sensitivity to punishment/reward in predicting negative driving outcomes (accident involvement and traffic offences) among young drivers. Gender and driver's age are additional factors examined in relation to driving outcomes and personality. Adopting the contextual mediated model of traffic accident involvement (Sümer, 2003), the study is based on the theory that personality, age and gender represent distal factors that predict accident involvement indirectly through their relationship with stable tendencies towards aberrant driving behavior. Results from correlations and Structural Equation Modeling using AMOS 6 indicated that direct personality effects on driving outcomes were few, whereas personality had significant correlations with aberrant driving behavior, showing that personality is a distal but important predictor of negative driving outcomes. These high risk traits appear to be at a peak among young male drivers. Thus, personality is important in understanding aggressive and risky driving by young adults and needs to be taken into consideration in designing targeted accident prevention policies.