Supercritical carbon dioxide extraction ofDimorphotheca pluvialis oil seeds

The possibility of extraction ofDimorphotheca oil with supercritical carbon dioxide is demonstrated in this article. Before extraction, the seeds have to be pretreated to improve extraction yield. Experiments showed that the best pretreatment procedure forDimorphotheca was heating the seeds under reduced pressure to 100°C for 60 min, followed by flaking or milling. To give an impression about the efficiency of the supercritical extraction, a mathematical model has been developed to estimate the overall mass transfer coefficient (A _p K). Also, an empirical relation betweenA _p K and the interstitial velocity has been found. The physical properties of the supercriticalDimorphotheca oil are in good agreement with those of conventionally extracted oil, except for a lower phospholipid content. It is expected that further refining of supercriticalDimorphotheca oil will be marginal.     

A unified approach to the recognition of complex actions from sequences of zone-crossings

We present a method for the recognition of complex actions. Our method combines automatic learning of simple actions and manual definition of complex actions in a single grammar. Contrary to the general trend in complex action recognition that consists in dividing recognition into two stages, our method performs recognition of simple and complex actions in a unified way. This is performed by encoding simple action HMMs within the stochastic grammar that models complex actions. This unified approach enables a more effective influence of the higher activity layers into the recognition of simple actions which leads to a substantial improvement in the classification of complex actions. We consider the recognition of complex actions based on person transits between areas in the scene. As input, our method receives crossings of tracks along a set of zones which are derived using unsupervised learning of the movement patterns of the objects in the scene. We evaluate our method on a large dataset showing normal, suspicious and threat behaviour on a parking lot. Experiments show an improvement of ~ 30% in the recognition of both high-level scenarios and their composing simple actions with respect to a two-stage approach. Experiments with synthetic noise simulating the most common tracking failures show that our method only experiences a limited decrease in performance when moderate amounts of noise are added.     

Taxonomic data integration from multilingual Wikipedia editions

Information systems are increasingly making use of taxonomic knowledge about words and entities. A taxonomic knowledge base may reveal that the Lago di Garda is a lake and that lakes as well as ponds, reservoirs, and marshes are all bodies of water. As the number of available taxonomic knowledge sources grows, there is a need for techniques to integrate such data into combined, unified taxonomies. In particular, the Wikipedia encyclopedia has been used by a number of projects, but its multilingual nature has largely been neglected. This paper investigates how entities from all editions of Wikipedia as well as WordNet can be integrated into a single coherent taxonomic class hierarchy. We rely on linking heuristics to discover potential taxonomic relationships, graph partitioning to form consistent equivalence classes of entities, and a Markov chain-based ranking approach to construct the final taxonomy. This results in MENTA (Multilingual Entity Taxonomy), a resource that describes 5.4 million entities and is one of the largest multilingual lexical knowledge bases currently available.     

Purification and preliminary characterization of a frog-derived proteinaceous chemoattractant eliciting prey attack by checkered garter snakes (Thamnophis marcianus)

A potent proteinaceous chemoattractant, eliciting prey attack by checkered garter snakes (Thamnophis marcianus) was isolated from aqueous washes of the common frogRana temporaria and purified by preparative continuous-elution electrophoresis. The biological activity of the frog crude extract or of the purified chemoattractive protein, measured by a snake bioassay, was unaffected by freezing, lyophilization, or dialysis but was lost after proteolytic digestion. The purified chemoattractant is glycosylated, has an apparent molecular mass of 24 kDa, estimated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE), and a pI of 4.8. It gave one spot in two-dimensional electrophoresis. The bioassay showed that this protein is highly attractive to snakes. The lowest concentration yielding positive responses in the snake bioassay was approximately 25 µg/ml. These results suggest that a water-soluble Mr 24 kDa glycoprotein molecule produced by the common frog may be a vomeronasal stimulus used by checkered garter snakes for prey recognition.     

Larvicidal Effect of a Cell-Wall Fraction Isolated from Alder Decaying Leaves

Decaying alder leaves in water from Alpine Aedes breeding sites, particularly their toxicity to larval Culicidae, were investigated and characterized with comparative toxicological and chemical methods. Bioassays that used third-instar Aedes aegypti as a reference species indicated that the larvicidal effect of crude leaf litter varied with decaying age of the litter, while no toxicity was detected from leaching water of the mosquito breeding sites. Ten-month-old leaf litter was the most toxic. Comparison of the different soluble and insoluble fractions obtained after sequential extraction of decomposed litter allowed us to localize the toxicity factor to an insoluble cell-wall fraction. The toxicity seems to be linked to phenolic activity. It is higher than that found for tannic acid solutions used as a reference to mimic the larvicidal effects of the molecules naturally occurring in decaying litter. The pattern of establishing the larvicidal effect of alder leaf litter in water of Alpine Aedes breeding sites is discussed.     

Spin coating of hydrophilic polymeric films for enhanced centrifugal flow control by serial siphoning

In this paper, we implement rotational flow control on a polymeric microfluidic “lab-on-a-disc” platform by combining serial siphoning and capillary valving for sequential release of a set of on-board stored liquid reagents into a common (assay) channel. The functionality of this integrated, multi-step, multi-reagent centrifugal assay platform critically depends on the capability to establish very reproducible, capillary-driven priming of the innately only weakly hydrophilic siphon microchannels made from common poly(methyl methacrylate) (PMMA) substrates. Due to the relatively high contact angle of the native PMMA substrate, it was practically impossible to ensure sequential release of on-board stored reagents using the capillary-driven serial siphon valves. In this work, we demonstrate that spin-coated hydrophilic films of poly(vinyl alcohol) (PVA) and (hydroxypropyl)methyl cellulose (HPMC) provide stable contact angles on PMMA substrates for more than 60 days. The deposited films were characterized using contact angle measurements, surface energy calculations and X-ray photoelectron spectroscopy spectra. The PVA and HPMC films reduced the water contact angle of the PMMA substrate from 68° to 22° and 27° while increasing their surface energies from 47 to 62 and 57 mN m^?1, respectively. On the centrifugal microfluidic platform, the films were validated to enable the effective and reproducible priming of the serial siphon microchannels at low rotational frequencies while ensuring that the in-line capillary valves are not opened until their respective burst frequencies are passed. Furthermore, the biocompatibility of the proposed surface modification method was examined, and the platform was used to run a sandwich immunoassay for the detection of human immunoglobulin G, and its performance was proven to be comparable to dynamic coating using surfactants.     

Introduction of disulfide bonds into Bacillus subtilis neutral protease

The effects of engineered disulfide bonds on autodigestion andthermostability of Bacillus subtilis neutral protease (NP-sub)were studied using site-directed mutagenesis. After modellingstudies two locations that might be capable of forming disulfidebonds, both near previously determined autodigestion sites inNP-sub, were selected for the introduction of cysteines. Analysisof mutant enzymes showed that disulfide bonds were indeed formedin vivo, and that the mutant enzymes were fully active. Theintroduced disulfides did not alter the autodigestion patternof the NP-sub. All mutant NP-subs exhibited decreased thermostability,which, by using reducing agents, was shown to be caused by theintroduction of the cysteines and not by the formation of thedisulfides. Mutants containing one cysteine exhibited intermoleculardisulfide formation at elevated temperatures, which, however,was shown not to be the cause of the decreased thermostability.Combining the present data with literature data, it would seemthat the introduction of disulfide bridges is unsuitable forthe stabilization of proteases. Possible explanations for thisphenomenon are discussed.     

A Cmos Interface for Thermocouples with Reference-Junction Compensation

This paper presents a CMOS interface for a thermocouple, includingreference-junction compensation. The interface contains a first-orderoscillator whose period is modulated by the signal from the thermocouple(V_X) and two other signals generated internally. One is abase-emitter voltage (V_BE) and the other is a PTAT voltage(V_PTAT). Linear combinations of the periods corresponding tothese two voltages are made by a µC, to obtain a reference signal anda temperature-dependent signal. The internal signals are required to provideauto-calibration for offset and gain and to measure the interface internaltemperature. Dynamic Element Matching is applied to generate an accurate andreliable PTAT voltage. The inaccuracy in the base-emitter voltage iscalibrated using a vertical PNP substrate transistor with multi-emitterareas. The applied modulator used in the circuit has a second-orderfiltering which suppresses low-frequency (1/f) noise. This filteringproperty enables the use of a low-cost CMOS process for the implementationof the circuit. The interface is able to measure a voltage(V_X) in the range of –27 mV to 100 mV, the measuredaccuracy of the system over a temperature range of –25°C to75°C is 550 ppm of V_X ± 2µV. The inaccuracyin determining the reference-junction temperature is 0.6 K. The measuringtime is 50 ms.     

Electrical Systems for Automatic Handling and Electric Heating of Slabs

The availability of large quantities of electrical power and sophisticated means of control has justified the use of induction methods in heating large steel slabs to rolling mill temperatures. Capable of delivering 210 MW, a unique power system, comprising power transformers, autotransformers, static switches, and capacitor banks, provides eighteen induction heaters with controlled electrical energy. The entire system can deliver 600 ton/h of steel to the rolling mill. The basic overall automatic control scheme involves the operations of slab handling control, heater control, static power switching, and computer control. The control functions, including phase balance and demand limit control, are described and discussed. The features and operation of the static power switch, capable of switching close to 10 000 A, are presented. Used as a protective device, the switch can clear up to 210 000-A faults in less than one cycle.     

An End to End Adaptable Architecture for Streaming Media over IP Networks

Wireless networks differ in bandwidth, size and access costs each requiring a set of protocol functions to enable devices to communicate efficiently. Portable multimedia devices such as PDA's and laptops will also vary greatly however all these devices will require optimal multimedia delivery. A traditional method is for sources to limit their transmission rates to accommodate lower bandwidth links, even though high-bandwidth connectivity is available to many participants. This method similar to others does not provide optimum throughput to heterogeneous clients due to its quest for a common denominator bandwidth. In addition, due to the divergence of users and applications, traditional protocol stacks are frequently enriched with additional functionality such as transport protocol functionality, synchronization and presentation coding which can lead to a performance bottleneck due to the insufficient processing power and memory of portable devices.Micro-protocols attempt to eradicate this bottleneck by optimising the protocol stack to the functionality that is actually required by the application. A side effect of this is that it allows a device such as a PDA to offer protocol functions, which would not normally be available due to its memory constraints achievable by downloading necessary micro-protocols for new environments and discarding previous micro-protocols. Multicast media groups overcome the heterogeneous client problem where clients subscribe to different quality of services in accordance with resource availability and move between groups according to bandwidth availability.Chameleon is 100% Java middleware for multimedia streaming to heterogeneous mobile clients, which allows the dynamic configuration of protocols with respect to application requirements and available network resources. We evaluate the dynamic reconfigurability of the middleware in order to demonstrate runtime adaptation. We especially concentrate on the primary quality transformation technique (PQT) of the middleware which enables clients to subscribe to media groups in accordance with available resources and network capacity.