Proteomics of plaques and novel sources of potential biomarkers for atherosclerosis

Cardiovascular disease (CVD) is the leading cause of death and loss of productive life years in the world. The underlying syndrome of CVD, atherosclerosis, is a complex disease process, which involves lipid metabolism, inflammation, innate and adaptive immunity, and many other pathophysiological aspects. Furthermore, CVD is influenced by genetic as well as environmental factors. Early detection of CVD and identification of patients at risk are crucial to reduce the burden of disease and to allow personalized treatment. As established risk factors fail to accurately predict which part of the population is likely to suffer from the disease, novel biomarkers are urgently needed. Proteomics can play a significant role in identifying these biomarkers. In this review, we describe the progress made in proteome profiling of the atherosclerotic plaque and several novel sources of potential biomarkers, including circulating cells and plasma extracellular vesicles. The importance of longitudinal biobanking in biomarker discovery is highlighted and exemplified by several plaque proteins identified in the biobank study Athero-Express. Finally, we discuss the PTMs of proteins that are involved in atherosclerosis, which may become one of the foci in the ongoing quest for biomarkers through proteomics of plaque and other matrices relevant to the progression of atherosclerosis.     

The design of a collision avoidance system for use by Pilots operating on the airport ramp and in taxiway areas

Ground collisions have serious implications from both a safety and a commercial perspective. This paper reports on human computer interaction (HCI) research related to the advancement of a collision avoidance system, for use by Pilots operating on the airport ramp and in taxiway areas. Primarily, this paper focuses on the key findings of this research and the emerging HCI design solution.     

Thin and thick YBCO films grown by MOCVD processes. Study of the angular magnetic field dependence of current transport properties

Thin and thick YBCO Films have been grown by Aerosol Assisted as well as by thermal MOCVD. The Aerosol Assisted MOCVD technique allows the growth of YBCO films from a single liquid source at deposition rates of up to 10m/h.Transport measurements (I-V) on etched microbridges using a single pulse technique have been performed. The angular magnetic field dependence J_c() of the critical current density from T_c down to 50 K has been measured. Transport properties are reported and discussed with respect to the microstructural features as determined by X-ray diffraction and electron microscopy.     

An Experimental Economics Investigation of Shipper-carrier Interactions in the Choice of Mode and Shipment Size in Freight Transport

The paper discusses the theoretical and empirical evidence on the subject and concludes that freight mode choice can be best understood as the outcome of interactions between shippers and carriers, and that mode choice depends to a large extent on the shipment size that results from shipper-carrier interactions. These conclusions are supported by economic experiments designed to test the hypothesis of cooperative behavior. This was accomplished by conducting two sets of experiments (ones with the shipper playing the lead role in selecting the shipment size; and others in which the shipment size decision was left to the carriers), and by comparing their results to the ones obtained numerically under the assumption of perfect cooperation. The comparison of results indicated that the experiments converged to the perfect cooperation case. This is in line with the conclusion from game theory that indicates that under typical market conditions the shipper and carrier would cooperate. These results also imply that it really does not matter who “makes” the decision about the shipment size and mode to be used at a given time period, as over time the shipper—that is the customer—ends up selecting the bids more consistent with its own interest. In other words, these results do not support the assumption that freight mode choice is solely made by the carriers.     

Customising agile methods to software practices at Intel Shannon

Tailoring of methods is commonplace in the vast majority of software development projects and organisations. However, there is not much known about the tailoring and engineering of agile methods, or about how these methods can be used to complement each other. This study investigated tailoring of the agile methods, eXtreme programming (XP) and Scrum, at Intel Shannon, and involved experienced software engineers who continuously monitored and reflected on these methods over a 3-year period. The study shows that agile methods may individually be incomplete in supporting the overall development process, but XP and Scrum complement each other well, with XP providing support for technical aspects and Scrum providing support for project planning and tracking. The principles of XP and Scrum were carefully selected (only six of the 12 XP key practices were implemented, for example) and tailored to suit the needs of the development environment at Intel Shannon. Thus, the study refutes the suggestion that agile methods are not divisible or individually selectable but achieve their benefits through the synergistic combination of individual agile practices; rather, this study shows that an a la carte selection and tailoring of practices can work very well. In the case of Scrum, some local tailoring has led to a very committed usage by developers, in contrast to many development methods whose usage is limited despite being decreed mandatory by management. The agile practices that were applied did lead to significant benefits, including reductions in code defect density by a factor of 7. Projects of 6-month and 1-year duration have been delivered ahead of schedule, which bodes well for future ability to accurately plan development projects.     

The Effects of Hippocampal Lesions on Response, Direction, and Place Learning in Rats.

Rats with hippocampal or sham lesions were trained to find food on a T maze located at 2 positions. Response rats were required to make a right or left turn. Direction rats were required to go in a consistent direction (east or west). Place rats were required to go to a consistent location, relative to room cues. One place group had distinguishable start points at the 2 maze positions, whereas another place group had start points facing the same side of the room. Controls took longer to solve a place problem than the response and direction problems when the start points were not distinguishable. Rats with hippocampal lesions were not different than controls on the response problem but were impaired on the direction and place problems. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Slip in Vibrating Wire Experiments on 3He-4He Mixtures

No Heading We present vibrating wire viscometer experiments in concentrated and dilute ³He-⁴He mixtures showing that the slip length may become orders of magnitude larger than the mean free path due to increased specular scattering of the ³He quasiparticles with the superfluid ⁴He film on the wire. The experimental results are in good agreement with a recent theory for slip which accounts for the cylindrical geometry of the wire and for velocity slip in directions normal as well as tangential to the surface of the wire.PACS numbers: 67.60.Fp, 51.20.+d     

夹杂物问题应力场的数值计算

等效夹杂物方法是细观力学中的一种重要方法，文中编制的数值计算程序能在考虑多个夹杂物相互响应的情况下计算材料应力、应变场，并实现了计算结果的三维显示．计算结果与文献中的计算结果非常吻合．本文编制的数值计算程序不仅可以用于研究复合材料的损伤演化及失效，而且利用特殊构型的椭球空洞来模拟裂纹，可以计算裂纹群的应力、应变场。     

Antigens and Antibodies of the Antiphospholipid Syndrome as New Allies in the Pathogenesis of COVID-19 Coagulopathy

High prevalence of both criteria and extra-criteria antiphospholipid antibodies (aPL) has been reported in COVID-19 patients. However, the differences in aPL prevalence decreased when an age-matched control group was included. The association of aPL with thrombotic events in COVID-19 is very heterogeneous. This could be influenced by the fact that most of the studies carried out were conducted on small populations enriched with elderly patients in which aPL was measured only at a single point and they were performed with non-standardized assays. The few studies that confirmed aPL in a second measurement showed that aPL levels hardly changed, with the exception of the lupus anticoagulant that commonly reduced. COVID-19 coagulopathy is an aPL-independent phenomenon closely associated with the onset of the disease. Thrombosis occurs later in patients with aPL presence, which is likely an additional prothrombotic factor. B2-glycoprotein deficiency (mainly aPL antigen caused both by low production and consumption) is very common during the SARS-CoV2 infection and has been associated with a greater predisposition to COVID-19 complications. This could be a new prothrombotic mechanism that may be caused by the blockage of its physiological functions, the anticoagulant state being the most important.     

Acute T-Cell-Driven Inflammation Requires the Endoglycosidase Heparanase-1 from Multiple Cell Types

It has been accepted for decades that T lymphocytes and metastasising tumour cells traverse basement membranes (BM) by deploying a battery of degradative enzymes, particularly proteases. However, since many redundant proteases can solubilise BM it has been difficult to prove that proteases aid cell migration, particularly in vivo. Recent studies also suggest that other mechanisms allow BM passage of cells. To resolve this issue we exploited heparanase-1 (HPSE-1), the only endoglycosidase in mammals that digests heparan sulfate (HS), a major constituent of BM. Initially we examined the effect of HPSE-1 deficiency on a well-characterised adoptive transfer model of T-cell-mediated inflammation. We found that total elimination of HPSE-1 from this system resulted in a drastic reduction in tissue injury and loss of target HS. Subsequent studies showed that the source of HPSE-1 in the transferred T cells was predominantly activated CD4⁺ T cells. Based on bone marrow chimeras, two cellular sources of HPSE-1 were identified in T cell recipients, one being haematopoiesis dependent and the other radiation resistant. Collectively our findings unequivocally demonstrate that an acute T-cell-initiated inflammatory response is HPSE-1 dependent and is reliant on HPSE-1 from at least three different cell types.