Modeling the Combining Site of the Human Asialoglycoprotein Receptor

A model for the carbohydrate recognition domain (CRD) and combining site of the human asialoglycoprotein (ASGP) receptor has been computed on the basis of the close sequence homology with the mannose-binding lectin (MBP), whose three-dimensional structure in complex with a ligand has been determined by crystallographic methods (Weis, W.I.; Drickamer, K.; Hendrickson, W.A. Nature 1992, 360: 127). Within the limitations of modeling methods, the model is compatible with data on ligand binding of the family C-type lectins, of which the MBP and the ASGP receptor are members. The model derived can serve as a guide for designing site-directed mutagenesis experiments in order to further elucidate the origins of specificity of the ASGP receptor toward galactose. In particular, the model focuses attention on the possible role of position 207 (MBP sequence numbering) in promoting galactose binding.     

Structural models of critical regions in old-type r.c. frames with smooth rebars

Structural assessment of existing reinforced concrete constructions under gravity loads and seismic actions has a high social and economical impact; actually in many European countries, most of the buildings dates back to 1960s and 1970s and cannot ensure satisfactory seismic response, since many areas have been later classified as seismic or since design has been carried out according to obsolete codes. These structures are generally reinforced with smooth bars that exhibit poor bond and need specific anchoring end details. In the present paper, some key aspects of structural models of smooth reinforcement for old-type r.c. frame analysis are reported. Results of experimental tests on smooth reinforcement and circular hook anchoring devices are also used to discuss some aspects of behavioural models of beam to column critical regions.     

The ideal reporting interval for an epidemic to objectively interpret the epidemiological time course

The reporting interval of infectious diseases is often determined as a time unit in the calendar regardless of the epidemiological characteristics of the disease. No guidelines have been proposed to choose the reporting interval of infectious diseases. The present study aims at translating coarsely reported epidemic data into the reproduction number and clarifying the ideal reporting interval to offer detailed insights into the time course of an epidemic. We briefly revisit the dispersibility ratio, i.e. ratio of cases in successive reporting intervals, proposed by Clare Oswald Stallybrass, detecting technical flaws in the historical studies. We derive a corrected expression for this quantity and propose simple algorithms to estimate the effective reproduction number as a function of time, adjusting the reporting interval to the generation time of a disease and demonstrating a clear relationship among the generation-time distribution, reporting interval and growth rate of an epidemic. Our exercise suggests that an ideal reporting interval is the mean generation time, so that the ratio of cases in successive intervals can yield the reproduction number. When it is impractical to report observations every mean generation time, we also present an alternative method that enables us to obtain straightforward estimates of the reproduction number for any reporting interval that suits the practical purpose of infection control.     

The effect of surface preparation on the bond interface between FRP sheets and concrete members

Fiber-reinforced polymer (FRP) composite wraps have been established as an effective method for rehabilitation and strengthening of concrete structures. They are being increasingly used as an alternative to steel for reinforcing and strengthening of concrete structures. This paper presents the experimental and analytical results of the influence of concrete surface treatment and the type of FRP sheets on the bonding strength of concrete-FRP sheet. The FRP sheets were bonded to concrete beams in two opposite sides using an epoxy resin. Variables included the type of fiber (C1, C5, and GE) and the surface treatment (water jet and sanding). With changing the surface treatment of concrete surface preparation and the type of fiber sheets, the bonding strength, bonding load–strain response and failure modes were investigated. The concrete specimens with surface roughened with water jet showed much better bonding strength than those roughened with an ordinary sander. Equations for predicting the bond load failure of concrete specimens externally bonded with carbon and glass fiber sheets compared well with those of experimental.     

Patenting and licensing by Spanish firms: Available survey evidence

This paper presents the results of the OEPM (Spanish Patent and Trademark Office)/OECD (Organisation for Economic Co-operation and Development) survey on the economic and financial uses of patents carried out in 2008 among Spanish business applicants of OEPM patents. It also summarizes evidence from previous surveys. All available information indicates that only a few Spanish firms holding patents license them out. Results from the OEPM/OECD survey also reveal that obtaining public support is the first financial use of patents for Spanish firms. Responses to the same OECD questionnaire by EPO patent applicants from several European countries are quite different: licensing out is an extended practice among EPO patent holders from different countries, including Spain, and convincing venture capitalists and private investors are their two most important financial uses of patents. In our view this suggests that there are significant differences across European countries as regards the development of national technology markets and results from international surveys are driven by responses from the largest and more developed countries. The drivers and actors in markets for patents that only protect inventions nationally may be quite distinct from those in markets for patents that protect inventions regionally, such as EPO patents within Europe.     

A microfluidic platform using molecular beacon-based temperature calibration for thermal dehybridization of surface-bound DNA

This work presents a simple microfluidic device with an integrated thin-film heater for studies of DNA hybridization kinetics and double-stranded DNA melting temperature measurements. The heating characteristics of the device were evaluated with a novel, noninvasive indirect technique using molecular beacons as temperature probes inside reaction chambers. This is the first microfluidic device in which thermal dehybridization of surface-bound oligonucleotides was performed for measurement of double-stranded DNA melting temperatures with +/- 1 degrees C precision. Surface modification and oligonucleotide immobilization were performed by continuously flowing reagents through the microchannels. The resulting reproducibility of oligonucleotide surface densities, at 9% RSD, was better than for the same modification chemistries on glass slides in unstirred reagent solutions (RSD=20%). Moreover, the surface density of immobilized DNA probe molecules could be varied controllably by changing the concentration of the reagent solution used for immobilization. Thus, excellent control of surface characteristics was made possible, something which is often difficult to achieve with larger devices. Solid-phase hybridization reactions, a fundamental aspect of microarray technologies often taking several hours in conventional systems, were reduced to minutes in this device. It was also possible to determine forward rate constants for hybridization, k. These varied from 820,000 to 72,000 M(-1) s(-1), decreasing as surface densities increased. Surface densities could therefore be optimized to obtain rapid hybridization using such an approach. Taken together, this combined microfluidic/small-volume heating approach represents a powerful tool for surface-based DNA analysis.     

The anti-symmetry principle for quasi-static crack propagation in Mode III

In this note we study a basic propagation criterion for quasi-static crack evolution in Mode III. Using classical techniques of complex analysis, the assumption of stable growth is expressed in terms of the parameters defining the elastic field around the tip. We explore the consequences of the local condition obtained and analyse its role as a crack propagation law. In particular, we herein extend to bounded domains a number of results previously obtained for the whole plane.