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European Food Research and Technology - 相似文献
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Wim van Eck 《Computers & Security》1985,4(4):269-286
This paper describes the results of research into the possibility of “eavesdropping” on video display units, by picking up and decoding the electromagnetic interference produced by this type of equipment. During the research project, which started in January, 1983, it became more and more clear that this type of information theft can be committed very easily using a normal TV receiver. 相似文献
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DR Sizemore EA Elsinghorst LC Eck AA Branstrom DL Hoover RL Warren FA Rubin 《Canadian Metallurgical Quarterly》1997,65(1):309-312
Human monocyte-derived macrophages (MDM) provided this laboratory with a tool to develop a primary-cell assay for evaluating the relative virulence of newly constructed Salmonella typhi carrier strains. In this study, the interaction with and survival within MDM were compared for delta aroA143-attenuated strains, wild-type virulent strains, and the current oral-vaccine strain, Ty21a. 相似文献
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Miriam Knie Kathrin Schoppmann Hendrik Eck Bernard Wolfschoon Ribeiro Christian Laforsch 《Microgravity science and technology》2016,28(3):345-349
The Drop Tower Bremen, a ground-based facility enabling research under real microgravity conditions, is an excellent platform for testing new types of experimental hardware to ensure full performance when deployed in costly and rare flight opportunities such as suborbital flights. Here we describe the “Daphnia” experiment which will fly on XCOR Aerospace Lynx Mark I and our experience from the hardware tests with the catapult system at the drop tower. The aim of the “Daphnia” experiment is to obtain data on the biological performance of daphnids and predator-prey interactions in microgravity, which are important for the development of aquatic bioregenerative life support systems (BLSS). The experiment consists of two subunits: The first unit is dedicated to predator-prey interactions, where behavioural analysis should reveal if microgravity interfere with prey (Daphnia) detection or feeding and therefore may interrupt the trophic cascade. The functioning of such an artificial food web is indispensable for a long-lasting BLSS suitable for long-duration manned space missions or Earth-based explorations to extreme habitats. The second unit is designed to investigate the impact of microgravity on gene expression and the cytoskeleton in Daphnia. Next to data collection, the real microgravity conditions at the drop tower have helped to identify the weak points of the “Daphnia” experimental hardware and lead to further improvement. Hence, the drop tower is ideal for testing new experimental hardware which is indispensable before the implementation in suborbital flights. 相似文献
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Adam Eck Leen-Kiat Soh Sam Devlin Daniel Kudenko 《Autonomous Agents and Multi-Agent Systems》2016,30(3):403-445
In this paper, we address the problem of suboptimal behavior during online partially observable Markov decision process (POMDP) planning caused by time constraints on planning. Taking inspiration from the related field of reinforcement learning (RL), our solution is to shape the agent’s reward function in order to lead the agent to large future rewards without having to spend as much time explicitly estimating cumulative future rewards, enabling the agent to save time to improve the breadth planning and build higher quality plans. Specifically, we extend potential-based reward shaping (PBRS) from RL to online POMDP planning. In our extension, information about belief states is added to the function optimized by the agent during planning. This information provides hints of where the agent might find high future rewards beyond its planning horizon, and thus achieve greater cumulative rewards. We develop novel potential functions measuring information useful to agent metareasoning in POMDPs (reflecting on agent knowledge and/or histories of experience with the environment), theoretically prove several important properties and benefits of using PBRS for online POMDP planning, and empirically demonstrate these results in a range of classic benchmark POMDP planning problems. 相似文献
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Waschutza Gero; Li Volkhart; Schafer Thomas; Schomburg Dietmar; Villmann Carmen; Zakaria Hayssam; Otto Bernd 《Protein engineering, design & selection : PEDS》1996,9(10):905-912
Insertion sites for cysteines with optimal stereochemistry forthe formation of unstrained disulfide bridges were identifiedin recombinant human interferon- (rhu-IFN-) by computer modelling.We have engineered two different disulfide cross-linked mutants,containing a pair of symmetry-related disulfide bonds, whichstabilize the N-termini of both monomers of the homodimenc protein.Mutations E7C and S69C allow the formation of an intramonomerdisuffide bond between helices A and D. In contrast, the A17Cand H111C mutations lead to a covalent cross-link between bothmonomers. The AB-loop is linked to helix F. The fluorescenceproperties of native and disulfide cross-linked proteins werestudied as a function of guanidine hydrochloride concentration.Melting temperatures (Tm) were calculated from the decreasein CD ellipticity at 220 nm. The induction of the antiviraleffect was measured using A549 fibroblast cells infected withencephalomyocarditis virus. The ability to induce the expressionof the HLA-DR antigen in Colo 205 cells was determined by fluorescence-activatedcell scanning analysis. The stability of both mutants was stronglyenhanced against temperature- and cosolvent-induced unfolding.The Tm of mutant IFN- E7C/S69C was 15°C. All measured biologicalactivities of this mutant were equal to wild type. In the caseof the other mutant IFN- A17C/H111C, the Tm value was 25°C.This mutation abolishes nearly the entire biological activity(<1%) with no detectable changes of secondary structure inthe CD spectrum. Our results illustrate the importance of theN-terminal helix A and the AB-loop for the unfolding pathwayand thermodynamic stability of rhu-IFN-. 相似文献