Ohmic‐assisted hydrodistillation of essential oils from Zataria multiflora Boiss (Shirazi thyme)

Ohmic‐assisted hydrodistillation (OAHD) is a combination of ohmic heating and distillation, and could be considered as a novel method for the extraction of essential oils. Major problems with traditional methods are long extraction time and lower purity of the extract. In this study, OAHD was applied as an economic and green technology for the extraction of essential oils from Zataria multiflora Boiss. (Shirazi thyme) aerial parts and the results were compared to those obtained from hydrodistillation (HD) as a conventional method. The results showed that OAHD method had the extraction time of 32.21 ± 2.59 min while this value was about 57.21 ± 2.33 min for hydrodistillation (HD). Scanning electron micrographs of thyme leaves showed a sudden eruption of essential oil glands and their surrounding area for OAHD samples. GC–MS analysis indicated that both methods of OAHD and HD can extract the same compounds.     

TP53 Targeted Deep Sequencing of Cell-Free DNA in Esophageal Squamous Cell Carcinoma Using Low-Quality Serum: Concordance with Tumor Mutation

Circulating cell-free DNA (cfDNA) is emerging as a potential tumor biomarker. CfDNA-based biomarkers may be applicable in tumors without an available non-invasive screening method among at-risk populations. Esophageal squamous cell carcinoma (ESCC) and residents of the Asian cancer belt are examples of those malignancies and populations. Previous epidemiological studies using cfDNA have pointed to the need for high volumes of good quality plasma (i.e., >1 mL plasma with 0 or 1 cycles of freeze-thaw) rather than archival serum, which is often the main available source of cfDNA in retrospective studies. Here, we have investigated the concordance of TP53 mutations in tumor tissue and cfDNA extracted from archival serum left-over from 42 cases and 39 matched controls (age, gender, residence) in a high-risk area of Northern Iran (Golestan). Deep sequencing of TP53 coding regions was complemented with a specialized variant caller (Needlestack). Overall, 23% to 31% of mutations were concordantly detected in tumor and serum cfDNA (based on two false discovery rate thresholds). Concordance was positively correlated with high cfDNA concentration, smoking history (p-value = 0.02) and mutations with a high potential of neoantigen formation (OR; 95%CI = 1.9 (1.11–3.29)), suggesting that tumor DNA release in the bloodstream might reflect the effects of immune and inflammatory context on tumor cell turnover. We identified TP53 mutations in five controls, one of whom was subsequently diagnosed with ESCC. Overall, the results showed that cfDNA mutations can be reliably identified by deep sequencing of archival serum, with a rate of success comparable to plasma. Nonetheless, 70% non-identifiable mutations among cancer patients and 12% mutation detection in controls are the main challenges in applying cfDNA to detect tumor-related variants when blindly targeting whole coding regions of the TP53 gene in ESCC.     

Influence of drying and extraction methods on yield and chemical composition of the essential oil of Satureja hortensis

The aerial parts of Satureja hortensis, cultivated in Iran (Research Station of Alborz, Karaj), were collected at the full-flowering stage and dried by three different drying methods (sun-drying, shade-drying and oven-drying at 45 °C). The essential oils of every treatment were obtained by hydro-distillation of the aerial parts. In addition, the essential oil of shade-dried sample was obtained by two other distillation methods (water- and steam-distillation and direct steam-distillation). The oils were analyzed by capillary GC and GC–MS. Statistical analysis showed no significant difference between oil yield (w/w) of the oven-dried sample (1.06%) compared to shade-dried (0.94%) and sun-dried (0.87%). The oil content of the shade-dried sample, obtained by hydro-distillation, was higher (0.94%) than that of the steam-distilled (0.27%). Twenty-three components were identified in the oil of S. hortensis in the different drying methods, including carvacrol (46.0–48.1%) and γ-terpinene (37.7–39.4%) as main components. Seventeen compounds were characterized in the oils of different distillation methods, including carvacrol (12.3–46%) and γ-terpinene (37.7–70.4%). Although the drying methods had no significant effect on oil composition of S. hortensis, the distillation changed the percentage of main components sharply (significant at 1%). The steam-distillation method produced the lowest amount of carvacrol and highest amount of γ-terpinene. The results showed that extraction by hydro-distillation gave the best results for S. hortensis, based on oil yield and carvacrol percentage.     

Repeated loading of soil containing granulated rubber and multiple geocell layers

Sandy soil/aggregate, such as might be required in a pavement foundation over a soft area, was treated by the addition of one or more geocell layers and granulated rubber. It was then subjected to cyclic loading by a 300 mm diameter plate simulative of vehicle passes. After an initial study (that established both the optimum depth of the uppermost geocell layer and of the geocell inter-layer spacing should be 0.2 times plate diameter), repeated loading was applied to installations in which the number of geocell layers and the presence or absence of shredded rubber layers in the backfill was changed. The results of the testing reveal the ability of the composite geocell-rubber-soil systems to ‘shakedown’ to a fully resilient behavior after a period of plastic deformation except when there is little or no reinforcement and the applied repeated stresses are large. When shakedown response is observed, then both the accumulated plastic deformation prior to a steady-state response being obtained and the resilient deformations thereafter are reduced. Efficiency of reinforcement is shown to decrease with number of reinforcement layers for all applied stress levels and number of cycles of applied loading. The use of granulated rubber layers are shown to reduce the plastic deformations and to increase the resilient displacements compared to the comparable non-rubber construction. By optimal use of geocells and granulated rubber, deformations can be reduced by 60–70% compared with the unreinforced case while stresses in the foundation soil are spread much more effectively. On the basis of the study, the concept of combining several geocell layers with shredded rubber reinforcement is recommended for larger scale trials and for economic study.     

A Novel 2.5–3.1 GHz Wide-Band Low-Noise Amplifier in 0.18 \upmu \hbox {m} CMOS

Aiming for the simultaneous realization of constant gain, accurate input and output impedance matching and minimum noise figure (NF) over a wide frequency range, the circuit topology and detailed design of wide broadband low noise amplifier (LNA) are presented in this paper. A novel 2.5–3.1 GHz wide-band LNA with unique characteristics has been presented. Its design and layout are done by TSMC 0.18  \(\upmu \hbox {m}\) technology. Common gate stage has been used to improve input matching. In order to enhance output matching and reduce the noise as well, a buffer stage is utilized. Mid-stages which tend to improve the gain and reverse isolation are exploited. The proposed LNA achieves a power gain of 15.9 dB, a NF of 3.5 dB with an input return loss less than \(-\) 11.6, output return loss of \(-\) 19.2 to \(-\) 19 and reverse isolation of \(-\) 38 dB. The LNA consumes 54.6 mW under a supply voltage of 2 V while having some acceptable characteristics.     

A Comprehensive Experimental Study on Finishing Aluminum Tube by Proposed UAMAF Process

In this paper, a new noncontact ultrasonic magnetic abrasive finishing mechanism is presented. An ultrasonic vibration producer is used to vibrate the permanent magnets. The ferromagnetic steel grits in the created magnetic field form a flexible finishing tool. To take advantage of cavitation collapse pressure, the finishing zone components are immersed in water. The present work also studies the effect of parameters, i.e., time duration for finishing and working gap between magnetic poles and the workpiece on the surface roughness (Ra). The microscopic pictures and the roughness profile diagrams demonstrate the advantages of the proposed method.     

Performance Analysis of Contact Baking of Flat Bread in an Indirect-Heating Oven

A significant portion of Iranian flat breads are produced in semi-industrial, indirect-heating ovens. Therefore, an efficient oven design and a proper selection of operating conditions are crucial to improve the product quality and reduce energy consumption. In the present study, a mathematical model is developed to simulate a semi-industrial, indirect-heating, continuous oven performance during contact baking of an Iranian flat bread, referred to as Taftoon. Individual modes of heat transfer are considered among various components of the baking system to estimate the system performance and the bread quality in terms of design and operating conditions. The predictions of this model are in good agreement with the experimental data. Numerical results indicate that conduction is the primary heat transfer mechanism. Furthermore, the effects of dough thickness, conveyer speed, and input air velocity on the quality of the bread are studied and appropriate ranges for the parameters are determined.     

An efficient method of designing dual‐ and wide‐band power dividers with arbitrary power division

Capability of microstrip nonuniform transmission lines (MNTLs) for construction of dual‐band and broadband unequal Wilkinson power dividers with arbitrary‐way, arbitrary frequency band operations, and arbitrary power divisions is evaluated. Also, the MNTL transformers are introduced for dual‐band/broadband matching of the unequal output impedances of the MNTL power divider with arbitrary output terminal impedances. The strip width of MNTLs is considered variable and is written as a truncated Fourier series expansion. To show the validity of the design procedure, three experimental MNTL Wilkinson power dividers, which are dual‐band two‐ and three‐way power dividers with different power divisions working at 1 and 3.4 GHz and one broadband equal power divider working from 0.4 to 1.8 GHz, have been designed and fabricated. In the first ones with power division of 1.5, outputs isolation and ports matching of less than ?30 dB are achieved. Next, an extended recombinant structure is presented for achieving three‐way MNTL power dividers with dual‐band operation. The measured isolation between outputs and ports matching are better than 30 dB and measured forward transmissions are between ?4.87 and ?5.45 in two passbands of the divider. Also, for the proposed broadband divider, the measured isolation between the outputs is better than 13 dB in 127% desired bandwidth. © 2012 Wiley Periodicals, Inc. Int J RF and Microwave CAE, 2013.     

In‐stream construction‐induced suspended sediment in riverine ecosystems

Construction activities within the wetted‐perimeter of rivers, referred to as in‐stream construction, are prevalent economically and environmentally motivated activities having direct interactions with sensitive lotic environments. Currently, there is a paucity of research related to in‐stream construction activities and their effects on aquatic ecosystems. In‐stream construction‐induced suspended sediment may result in harmful effects to aquatic flora and fauna. Regulatory frameworks worldwide focus primarily on concentration, with limited consideration for duration and no consideration for spatial extents of suspended sediment exposures. This research develops theoretical concentration, duration, and spatial extent exposure risk relationships for riverine ecosystems to demonstrate the influence of each mechanism during typical in‐stream construction activities. To reduce exposure risk, concentration and duration may be considered pragmatically, based on anticipated activity characteristics and site conditions. Spatial exposure characteristics are important to consider, as illustrated by our finding that activities located near the channel centerline may result in greater exposure risk than similar activities conducted near the streambank. Current regulatory frameworks worldwide do not sufficiently consider all exposure risk mechanisms present during in‐stream construction‐induced suspended sediment releases, possibly inhibiting efforts to reduce adverse environmental effects. This research improves our understanding of suspended sediment in lotic environments and may help environmental managers better evaluate and manage in‐stream construction activities.