Exotic droplets formed in microfluidic chips with uniform wettability

Microdroplets are widely used in industrial processes. In the absence of external forces, droplets generally assume a spherical shape. Here, we present two methods to form non-spherical droplets in microfluidic glass chips. A uniformly hydrophobized chip has been successfully used to dynamically control the width and length of elongated water-in-oil droplets; on a hydrophilic chip, we generated non-spherical double droplets that consisted of two different oils in a water continuous phase.     

Use of life cycle assessments to evaluate the environmental footprint of contaminated sediment remediation

Ecological and human risks often drive the selection of remedial alternatives for contaminated sediments. Traditional human and ecological risk assessment (HERA) includes assessing risk for benthic organisms and aquatic fauna associated with exposure to contaminated sediments before and after remediation as well as risk for human exposure but does not consider the environmental footprint associated with implementing remedial alternatives. Assessment of environmental effects over the whole life cycle (i.e., Life Cycle Assessment, LCA) could complement HERA and help in selecting the most appropriate sediment management alternative. Even though LCA has been developed and applied in multiple environmental management cases, applications to contaminated sediments and marine ecosystems are in general less frequent. This paper implements LCA methodology for the case of the polychlorinated dibenzo-p-dioxins and -furans (PCDD/F)-contaminated Grenland fjord in Norway. LCA was applied to investigate the environmental footprint of different active and passive thin-layer capping alternatives as compared to natural recovery. The results showed that capping was preferable to natural recovery when analysis is limited to effects related to the site contamination. Incorporation of impacts related to the use of resources and energy during the implementation of a thin layer cap increase the environmental footprint by over 1 order of magnitude, making capping inferior to the natural recovery alternative. Use of biomass-derived activated carbon, where carbon dioxide is sequestered during the production process, reduces the overall environmental impact to that of natural recovery. The results from this study show that LCA may be a valuable tool for assessing the environmental footprint of sediment remediation projects and for sustainable sediment management.     

Testing Methods for PUF-Based Secure Key Storage Circuits

Design for test is an integral part of any VLSI chip. However, for secure systems extra precautions have to be taken to prevent that the test circuitry could reveal secret information. This paper addresses secure test for Physical Unclonable Function based systems. It investigates two secure Built-In Self-Test (BIST) solutions for Fuzzy Extractor (FE) which is the main component of PUF-based systems. The schemes target high stuck-at-fault (SAF) coverage by performing scan-chain free functional testing, to prevent scan-chain abuse for attacks. The first scheme reuses existing FE blocks (for pattern generation and compression) to minimize the area overhead, while the second scheme tests all the FE blocks simultaneously to minimize the test time. The schemes are integrated in FE design and simulated; the results show that for the first test scheme, a SAF fault coverage of 95 % can be realized with no more than 47.1k clock cycles at the cost of a negligible area overhead of only 2.2 %; while for the second test scheme a SAF fault coverage of 95 % can be realized with 3.5k clock cycles at the cost of 18.6 % area overhead. Higher fault coverages are possible to realize at extra cost (i.e., either by extending the test time, or by adding extra hardware, or a combination of both).     

Borosilicate nanoparticles prepared by exothermic phase separation

Nanoparticles play an important role in chemical and biological sciences due to their ability to bind and concentrate many molecules on their surface. Polymers and silica are widely used to make nanoparticles, but efforts to make nanoparticles from borosilicate glass--which exhibits high tolerance to chemicals and solvents, combined with excellent mechanical and thermal stability--have proved unsuccessful. Here we show that borosilicate nanoparticles (100-500 nm in size) can be synthesized by simply mixing a silicon-boron binary oxide solution, prepared using non-aqueous organic solvents, with water. This induces a vigorous exothermic phase separation in which borosilicate nanoparticles burst out of a silica phase. In addition to potential applications in the life sciences, monodisperse borosilicate particles could also have applications in the production of photonic bandgap devices with high optical contrast, contrast agents for ultrasonic microscopy or chemical filtration membranes.     

Neighbourhood ties and employment: a test of different hypotheses across neighbourhoods

Abstract

This study examines to which extent neighbourhood ties relate to employment status for the less-well educated inhabitants of 71 neighbourhoods in the Dutch city of Rotterdam. Previous research has produced different expectations as to whether having contact with neighbours is either positively or negatively related to being employed and how this relation differs across neighbourhoods. Two waves from the Neighbourhood Profile survey (N?=?8507) were used, which included measures of the contact frequency with neighbours and their willingness to help. We find that for the less-well educated neighbourhood ties have a modest negative relation to employment. Moreover, this relation does not vary across neighbourhoods with different socioeconomic statuses, with the exception of part-time working men. Our research implies that neighbourhood ties in mixed neighbourhoods do not positively relate to employment for the less-well educated, thereby questioning policy assumptions about ‘social mix’. Contributions to the field of neighbourhood studies are made by employing measures of the social networks mechanism and taking into account the conditionality of effects across neighbourhoods.     

Estimating the angle of attack from blade pressure measurements on the National Renewable Energy Laboratory phase VI rotor using a free wake vortex model: yawed conditions

Wind turbine design codes for calculating blade loads are usually based on a blade element momentum (BEM) approach. Since wind turbine rotors often operate in off‐design conditions, such as yawed flow, several engineering methods have been developed to take into account such conditions. An essential feature of a BEM code is the coupling of local blade element loads with an external (induced) velocity field determined with momentum theory through the angle of attack. Local blade loads follow directly from blade pressure measurements as performed in the National Renewable Energy Laboratory (NREL) phase IV campaign, but corresponding angles of attack cannot (on principle) be measured. By developing a free wake vortex method using measured local blade loads, time‐dependent angle of attack and induced velocity distributions are reconstructed. In a previous paper, a method was described for deriving such distributions in conjunction with blade pressure measurements for the NREL phase VI wind turbine in axial (non‐yawed) conditions. In this paper, the same method is applied to investigate yawed conditions on the same turbine. The study considered different operating conditions in yaw in both attached and separated flows over the blades. The derived free wake geometry solutions are used to determine induced velocity distributions at the rotor blade. These are then used to determine the local (azimuth time dependent) angle of attack, as well as the corresponding lift and drag for each blade section. The derived results are helpful to develop better engineering models for wind turbine design codes. Copyright © 2008 John Wiley & Sons, Ltd.     

Poly(ethylene glycol)-based stable isotope labeling reagents for the quantitative analysis of low molecular weight metabolites by LC-MS

Stable isotope labeling (SIL) in combination with liquid chromatography-mass spectrometry is one of the most widely used quantitative analytical methods due to its sensitivity and ability to deal with extremely complex biological samples. However, SIL methods for metabolite analysis are still often limited in terms of multiplexing, the chromatographic properties of the derivatized analytes, or their ionization efficiency. Here we describe a new family of reagents for the SIL of primary amine-containing compounds based on pentafluorophenyl-activated esters of 13C-containing poly(ethylene glycol) chains (PEG) that addresses these shortcomings. A sequential buildup of the PEG chain allowed the introduction of various numbers of 13C atoms opening extended multiplexing possibilities. The PEG derivatives of rather hydrophilic molecules such as amino acids and glutathione were successfully retained on a standard C18 reversed-phase column, and their identification was facilitated based on m/z values and retention times using extracted ion chromatograms. The mass increase due to PEG derivatization moved low molecular weight metabolite signals out of the often noisy, low m/z region of the mass spectra, which resulted in enhanced overall sensitivity and selectivity. Furthermore, elution at increased retention times resulted in efficient electrospray ionization due to the higher acetonitrile content in the mobile phase. The method was successfully applied to the quantification of intracellular amino acids and glutathione in a cellular model of human lung epithelium exposed to cigarette smoke-induced oxidative stress. It was shown that the concentration of most amino acids increased upon exposure of A549 cells to gas-phase cigarette smoke with respect to air control and cigarette smoke extract and that free thiol-containing species (e.g., glutathione) decreased although disulfide bond formation was not increased. These labeling reagents should also prove useful for the labeling of peptides and other compounds containing primary amine functionalities.