Long-Term Measures of Dyslipidemia,Inflammation, and Oxidative Stress in Rats Fed a High-Fat/High-Fructose Diet

Inflammation and oxidative stress are thought to be involved in, or associated with, the development of obesity, dyslipidemia, hepatic steatosis, and insulin resistance. This work was designed to determine the evolution of inflammation and oxidative stress during onset and progression of hepatic steatosis and glucose intolerance. Seventy-five male Wistar rats were divided to control and high-fat high-fructose (HFHFr) groups. A subgroup of each group was sacrificed at 4, 8, 12, 16, and 20 weeks. HFHFr-fed rats exhibited overweight, glucose intolerance, and hepatic steatosis with increased contents of hepatic diacylglycerols and ceramides. The HFHFr diet increased hepatic interleukin 6 (IL-6) protein and adipose tissue CCL5 gene expression and hepatic nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity but not mitochondrial reactive oxygen species (ROS) production. The HFHFr diet decreased plasma and liver levels of isoprostanoid metabolites as well as plasma thiobarbituric acid-reactive substance (TBARS) levels. Hepatic glutathione content was decreased with a moderate decrease in superoxide dismutase (SOD) and glutathione peroxidase (GPx) with the HFHFr diet. Overall, HFHFr diet led to hepatic lipid accumulation and glucose intolerance, which were accompanied by only moderate inflammation and oxidative stress. Most of these changes occurred at the same time and as early as 8 or 12 weeks of diet treatment. This implies that oxidative stress may be the result, not the cause, of these metabolic alterations, and suggests that marked hepatic oxidative stress should probably occur at the end of the steatotic stage to result in frank insulin resistance and steatohepatitis. These findings need to be further evaluated in other animal species as well as in human studies.     

Comparative study of purine and pyrimidine nucleoside analogues acting on the thymidylate kinases of Mycobacterium tuberculosis and of humans

Thymidine monophosphate kinase (TMPK) from Mycobacterium tuberculosis (TMPKmt) is an attractive target for the design of specific inhibitors. This fact is the result of its key role in the thymidine pathway and of unique structural features in the active site observed by X-ray crystallography, especially in comparison to its human counterpart (TMPKh). Different 5-modified thymidine derivatives, as well as purine and pyrimidine analogues or C-nucleosides were tested on TMPKmt and TMPKh, and the results were rationalized by docking studies. 5-Halogenated 2'-deoxyuridines are the best inhibitors of TMPKmt found and present the highest selectivity indexes in favor of TMPKmt.     

Influence of Host-Plant Surface Chemicals on the Oviposition of the Cereal Stemborer Busseola Fusca

The chemical composition of plant surfaces plays a role in selection of host plants by herbivorous insects. Once the insect reaches the plant, these cues determine host acceptance. Laboratory studies have shown that the stem borer Busseola fusca (Lepidoptera: Noctuidae), an important pest of sorghum and maize in sub-Saharan Africa, is able to differentiate between host and non-host plant species. However, no information is available on the cues used by this insect to seek and accept the host plant. Thus, the role of surface phytochemical stimuli on host selection and oviposition by B. fusca was studied in the laboratory using two host plants, sorghum, Sorghum bicolor, and maize, Zea mays, and one non-host plant, Napier grass, Pennisetum purpureum. The numbers of eggs and egg masses deposited on the three plant species were compared first under no-choice and choice conditions. In both cases, more eggs and egg masses were laid on maize and sorghum than on the non-host. Artificial surrogate stems treated with a water or chloroform surface extract of each plant were then compared with surrogate stems treated with, respectively, water or chloroform as controls, under similar conditions. Surrogate stems treated with plant water extracts did not show an increase in oviposition when compared to controls, indicating that the major compounds in these extracts, i.e., simple sugars and free amino acids, are not significantly responsible for the oviposition preference. By contrast, a chloroform extract of sorghum enhanced oviposition on the surrogate stems compared to the control, while those of maize and Napier grass showed no significant effects. Analysis of the chloroform extract of sorghum showed higher amounts of α-amyrin, ß-amyrin, and n-nonacosane compared to those of maize and Napier grass. A blend of the three chemicals significantly increased oviposition compared to the chloroform-treated control, indicating that these compounds are part of the surface chemical signature of the plant responsible for host recognition and oviposition by B. fusca.     

Towards efficient data distribution on computational desktop grids with BitTorrent

Datacentric applications are still a challenging issue for large-scale distributed computing systems. The emergence of new protocols and software for collaborative content distribution over the Internet offers a new opportunity for efficient and fast delivery of a high volume of data. This paper presents an evaluation of the BitTorrent protocol for computational desktop grids. We first present a prototype of a generic subsystem dedicated to data management and designed to serve as a building block for any desktop grid system. Based on this prototype we conduct experiments to evaluate the potential of BitTorrent compared to a classical approach based on FTP data server. The preliminary results obtained with a 65-node cluster measure the basic characteristics of BitTorrent in terms of latency and bandwidth and evaluate the scalability of BitTorrent for the delivery of large input files. Moreover, we show that BitTorrent has a considerable latency overhead compared to FTP but clearly outperforms FTP when distributing large files or files to a high number of nodes. Tests on a synthetic application show that BitTorrent significantly increases the communication/computation ratio of the applications eligible to run on a desktop grid system.     

On Topological Watersheds

In this paper, we investigate topological watersheds (Couprie and Bertrand, 1997). One of our main results is a necessary and sufficient condition for a map G to be a watershed of a map F, this condition is based on a notion of extension. A consequence of the theorem is that there exists a (greedy) polynomial time algorithm to decide whether a map G is a watershed of a map F or not. We introduce a notion of separation between two points of an image which leads to a second necessary and sufficient condition. We also show that, given an arbitrary total order on the minima of a map, it is possible to define a notion of degree of separation of a minimum relative to this order. This leads to a third necessary and sufficient condition for a map G to be a watershed of a map F. At last we derive, from our framework, a new definition for the dynamics of a minimum.Gilles Bertrand received his Ingénieurs degree from the École Centrale des Arts et Manufactures in 1976. Until 1983 he was with the Thomson-CSF company, where he designed image processing systems for aeronautical applications. He received his Ph.D. from the École Centrale in 1986. He is currently teaching and doing research with the Laboratoire Algorithmique et Architecture des Systémes Informatiques, ESIEE, Paris, and with the Institut Gaspard Monge, Université de Marne-la-Vallée. His research interests are image analysis, discrete topology and mathematical morphology.     

Measurements of the behavioral effects of albino mutation in mice (Mus musculus): Comparisons of coisogenic inbred and hybrid lines.

The effects of the albino gene on mouse behavior were examined, particularly its possible interactions with nonallelic genes (epistasis). More generally, the possible effects of genetic background (inbreeding depression or hybrid vigor) on the effects of the mutation were also considered. Tasks requiring either predominantly motor or predominantly cognitive capacity were studied for coisogenic albino and pigmented mice from either an inbred strain (C57BL/6 c/c vs C57BL/6 +/c) or an F? heterozygous generation (F? c/c vs F? +/c) from a BALB/c?×?C57BL/6 +/c cross. Results show a clear albino gene effect in the 2 lines and provide further evidence that the gene is the effective factor. There was no significant interaction between the mutation and the genotypic group (C57BL/6 or F?), which indicates that the effects of the mutation act approximately in an additive fashion between loci in these groups. (13 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Ahead of time static analysis for automatic generation of debugging interfaces to the Linux kernel

The Linux kernel does not export a stable, well-defined kernel interface, complicating the development of kernel-level services, such as device drivers and file systems. While there does exist a set of functions that are exported to external modules, this set of functions frequently changes, and the functions have implicit, ill-documented preconditions. No specific debugging support is provided. We present Diagnosys, an approach to automatically constructing a debugging interface for the Linux kernel. First, a designated kernel maintainer uses Diagnosys to identify constraints on the use of the exported functions. Based on this information, developers of kernel services can then use Diagnosys to generate a debugging interface specialized to their code. When a service including this interface is tested, it records information about potential problems. This information is preserved following a kernel crash or hang. Our experiments show that the generated debugging interface provides useful log information and incurs a low performance penalty.     

EM procedures using mean field-like approximations for Markov model-based image segmentation

Image segmentation using Markov random fields involves parameter estimation in hidden Markov models for which the EM algorithm is widely used. In practice, difficulties arise due to the dependence structure in the models and approximations are required. Using ideas from the mean field approximation principle, we propose a class of EM-like algorithms in which the computation reduces to dealing with systems of independent variables. Within this class, the simulated field algorithm is a new stochastic algorithm which appears to be the most promising for its good performance and speed, on synthetic and real image experiments.     

Formal verification of conflict detection algorithms

Safety assessment of new air traffic management systems is a main issue for civil aviation authorities. Standard techniques such as testing and simulation have serious limitations in new systems that are significantly more autonomous than the older ones. In this paper, we present an innovative approach for establishing the correctness of conflict detection systems. Fundamental to our approach is the concept of trajectory, and how we represent a continuous physical trajectory by a continuous path in the x-y plane constrained by physical laws and operational requirements. From the model of trajectories, we extract, and formally prove, high-level properties that can serve as a framework to analyze conflict scenarios. We use the AILS (Airborne Information for Lateral Spacing) alerting algorithm as a case study of our approach. Published online: 19 November 2002     

Magnebike: A magnetic wheeled robot with high mobility for inspecting complex‐shaped structures

This paper describes the Magnebike robot, a compact robot with two magnetic wheels in a motorbike arrangement, which is intended for inspecting the inner casing of ferromagnetic pipes with complex‐shaped structures. The locomotion concept is based on an adapted magnetic wheel unit integrating two lateral lever arms. These arms allow for slight lifting off the wheel in order to locally decrease the magnetic attraction force when passing concave edges, as well as laterally stabilizing the wheel unit. The robot has the main advantage of being compact (180 × 130 × 220 mm) and mechanically simple: it features only five active degrees of freedom (two driven wheels each equipped with an active lifter stabilizer and one steering unit). The paper presents in detail design and implementation issues that are specific to magnetic wheeled robots. Low‐level control functionalities are addressed because they are necessary to control the active system. The paper also focuses on characterizing and analyzing the implemented robot. The high mobility is shown through experimental results: the robot not only can climb vertical walls and follow circumferential paths inside pipe structures but it is also able to pass complex combinations of 90‐deg convex and concave ferromagnetic obstacles with almost any inclination regarding gravity. It requires only limited space to maneuver because turning on the spot around the rear wheel is possible. This high mobility enables the robot to access any location in the specified environment. Finally the paper analyzes the maximum payload for different types of environment complexities because this is a key feature for climbing robots and provides a security factor about the risk of falling and slipping. © 2009 Wiley Periodicals, Inc.