Structural properties of silver doped hydroxyapatite and their biocompatibility

The aim of this study was to obtain a novel hydroxyapatite-based material with high biocompatibility. The structural properties of the samples were well characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and X-ray Photoelectron Spectroscopy (XPS). The X-ray diffraction studies revealed the characteristic peaks of hydroxyapatite in each sample. Other phases or impurities were not observed. The scanning electron microscopy observations suggest that the doping components have no influence on the surface morphology of the samples, which reveals a homogeneous aspect of the synthesized particles for all samples. The presence of calcium (Ca), phosphor (P), oxygen (O) and silver (Ag) in the Ag:HAp is confirmed by energy dispersive X-ray (EDAX) and X-ray Photoelectron Spectroscopy analyses. Nanocrystalline silver doped HAp stimulated viability and potentiated the activation of murine macrophages.     

Synthesis of novel DC-SIGN ligands with an alpha-fucosylamide anchor

The dendritic cell-specific intercellular adhesion molecule (ICAM) 3-grabbing nonintegrin (DC-SIGN) is a C-type lectin that appears to perform several different functions. Besides mediating adhesion between dendritic cells and T lymphocytes, DC-SIGN recognizes several pathogens some of which, including HIV, appear to exploit it to invade host organisms. The intriguing diversity of the roles attributed to DC-SIGN and their therapeutic implications have stimulated the search for new ligands that could be used as biological probes and possibly as lead compounds for drug development. The natural ligands of DC-SIGN consist of mannose oligosaccharides or fucose-containing Lewis-type determinants. Using the known 3D structure of the Lewis-x trisaccharide, we have identified some monovalent alpha-fucosylamides that bind to DC-SIGN with inhibitory constants 0.4-0.5 mM, as determined by SPR, and have characterized their interaction with the protein by STD NMR spectroscopy. This work establishes for the first time alpha-fucosylamides as functional mimics of chemically and enzymatically unstable alpha-fucosides and describes interesting candidates for the preparation of multivalent systems able to block the receptor DC-SIGN with high affinity and with potential biomedical applications.     

Fruit production and consumption: practices,preferences and attitudes of women in rural western Kenya

This study identified practices, preferences, attitudes and decision-making for fruit consumption, fruit production, and use of income generated from these activities and assessed the contribution of fruits to closing nutritional gaps of members of smallholder farming households in Western Kenya during two different seasons. Two cross-sectional surveys were conducted, one in July/August 2013 (S1) and the other in February/March 2014 (S2). The same respondents, women responsible for food preparation in the household, were interviewed during S1 and S2 with a total of 272 women participating. Semi-structured questionnaires were used to collect data on practices, preferences and attitudes related to fruit production and consumption including a quantitative 24-h recall. On the day prior to the interview, only 25% of women during S1 and 37% during S2 had consumed fruits. Fruit consumption in grams per day differed significantly (p < 0.001) between the two seasons and was higher in S2 (mean = 106 g/d; SD = 208 g/d) compared to S1 (mean = 49 g/d; SD = 101 g/d). During both seasons, 80% or more respondents did not reach the recommended amount of 200 g of fruits per day and also had low vitamin A and C intakes, especially during S1. Simultaneously, 80% of women indicated that the household grew fruit, yet fruit production and consumption diversity were low. About 20% of respondents identified fruit consumption taboos for different household members and 37% viewed the consumption of wild fruits negatively. Integrated agriculture-nutrition programs should consider local practices, preferences and cultural attitudes in order to promote the consumption of a diversity of nutrient rich fruits and consequently better diets and nutrition.     

Stability of a chitosan layer deposited onto a polyethylene surface

A two‐step procedure was applied to obtain antimicrobial films; this procedure involved a corona treatment of the polyethylene (PE) surface and its chemical activation with 1‐ethyl‐3‐[3‐dimethylaminopropyl] carbodiimide hydrochloride and N‐hydroxysuccinimide, and this led to the covalent bonding of chitosan on the PE surface. Electrochemical methods were used to investigate the stability of the deposited chitosan layer. The potentiometric and polyelectrolyte titrations showed that some amount of chitosan desorbed faster from the surface until equilibrium was reached and also that the grafted chitosan layer was more stable than the physically adsorbed one. The chitosan immobilized on the PE surface exhibited the expected antibacterial activity when tested against three bacteria, which included two Gram‐negative bacteria, Salmonella enteritidis and Escherichia coli, and one Gram‐positive bacterium, Listeria monocytogenes. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 2444–2457, 2013     

TG/FTIR/MS study on the influence of nanoparticles content upon the thermal decomposition of starch/poly(vinyl alcohol) montmorillonite nanocomposites

The effect of the nanoclay content on the thermal decomposition of nanocomposites based on poly(vinyl alcohol)/thermoplastic starch, as intercalated hybrids, has been established. The changes in the decomposition products distribution and their evolution have been investigated by coupled thermogravimetric analysis, Fourier transform infrared spectroscopy, and mass spectrometry. Detailed analysis of the in situ vapor phase showed that the poly(vinyl alcohol)/starch/clay nanocomposites display a completely different distribution pattern of degradation product, depending on nanoclay content. By in situ vapor phase FTIR and MS spectroscopic techniques, both decomposition compounds of the constituent polymers and some new ones, depending on the nanoparticles content, are identified. The effect of the increase in nanoparticles content consists mainly in variation of some volatile compounds evolution, such as formic acid, water, formaldehyde, propionic acid, methanol, acetic acid carbon dioxide, benzene, etc., which in the case of nanocomposites is very complex. Thus, a content of 2–4 wt% organically-modified montmorillonite hinders the decomposition of the poly(vinyl alcohol)/starch blend. Both characteristic temperature of evolution of the main compounds increases by increasing its content and evolution starting time is delayed; while the untreated nanoclay acts like a catalyst, which decreases characteristic temperatures and evolution time with increasing its content. The temperature dependence of the maximum evolution rate of various compounds on the nanoclay content is very complex as, in the case of nanocomposites, of both primary and secondary reactions and transport phenomena occur simultaneously. Generally, this behavior is related to the dispersion of nanoclays in the polymeric matrix.     

Effect of the surface modification of the synthetic meshes used in the surgical treatment of pelvic organ prolapse on the tissue adhesion and clinical functionality

Stress urinary incontinence and pelvic organ prolapse represent important conditions affecting adult women’s health. Lately, the properties and performance of the polymer materials used in the treatment of pelvic organ prolapse have been improved by modulating from their formula. Materials investigated in our study are polypropylene meshes used by clinicians in surgical treatment of urinary incontinence and utero-vaginal prolapse. In order to make a comparative analysis, we confronted the structure and surface morphology of native synthetic polypropylene meshes with explanted fragments from the same mesh type after clinical use. FTIR investigations revealed little change in structure after implantation, somehow insignificant. Scanning electron microscopy (SEM) demonstrates modification in surface morphology even just by passing through the transobturator passage, whereas after implantation the surface deteriorates. SEM on explanted meshes proves the encapsulation process of the mesh to be more similar to a foreign body reaction rather than to the adhesion and integration of the synthetic mesh into the organism. Histological analysis of the surrounding tissues near explanted mesh was made in some relevant clinical cases, because the tissue response is an important factor in order to evaluate the tissue adhesion at the polypropylene mesh surface. Based on our experiments and clinical experience we’ve designed an adhesion score. It applies to meshes used in pelvic organ prolapse surgery measuring the following important factors: tenacity, extent of adhesion, macroscopic and microscopic aspects and ease of dissection from surrounding tissues.     

Development of a stability intelligent control system for turning

This paper presents a stability control system based on a new strategy, with application in turning. It works by permanent assessment of the system operating point position relative to the stability limit, and process parameter permanent, in-process modification such as this point is always placed in the stable domain zone that gives the highest process performance. In the case of turning, here approached: (1) this zone is the stability limit proximity; (2) the system operating point position is determined by assessing a cutting force monitored signal feature; and (3) this position is changed by modifying the cutting edge setting angle, the feed rate, and the cutting speed, as it follows: when the risk of overpassing the stability limit is imminent, the setting angle is increased, followed by a feed rate diminishing and then by a worked piece rotation speed reduction, while immediately after surpassing the risk, the three variables modification is reversed, this way the chatter onset being permanently avoided and the performance kept in every moment at the highest possible level. The system was experimentally implemented on a transversal lathe. The results of tests on dedicatedly designed specimens are showing a machining productivity significant increase, in conditions of a stable cutting process. The system is simple, and it can be easily added to the existing CNC machine tools, without important modifications.