Ultrasonic hydrophone based on short in-fiber bragg gratings

We investigate the feasibility of using in-fiber Bragg gratings for measuring acoustic fields in the megahertz range. We found that the acoustic coupling from the ultrasonic field to the grating leads to the formation of standing waves in the fiber. Because of these standing waves, the system response is complex and, as we show, the grating does not act as an effective probe. However, significant improvement in its performance can be gained by use of short gratings coupled with an appropriate desensitization of the fiber. A noise-limited pressure resolution of approximately 4.5 x 10(-3) atm/ radicalHz was found.     

Performance of an optoelectronic neural network in the presence of noise

Optoelectronic neural networks must not only be highly parallel but also fast to compete with electrical systems. Receiver noise becomes an important consideration at high data rates; so the limits set by noise to network size and speed are analyzed. A network incorporating an array of high-speed multi-quantum-well modulators was constructed. It employed a general method for optical representation of bipolar values, which required only a minimal increase in network dimensions and gave the network immunity to common-mode parameter variations. Different ways of partitioning pattern-recognition problems were compared, and the accuracy of one configuration was tested with the experimental network over a range of noise levels.     

Critical role of CD28/B7 costimulation in the development of human Th2 cytokine-producing cells

CD28 is a major costimulatory signal receptor for T cells. We have used human naive CD4+ cells from cord blood to analyze the effect of the CD28/B7 costimulatory pathway on development of T helper (Th) subsets. We show that CD28 costimulation is critical for development of the Th2 cytokine-producing cells and that in the absence of CD28 costimulation, cells are not primed to produce Th2 cytokines and consequently "default" to the Th1 subset, independent of the presence of exogenous cytokines. After CD28 costimulation, cells differentiate into a subset that produces Th2 cytokines. However, further CD28 costimulation is not required to maintain Th2 cytokine production. We conclude that D28 costimulation is critical for the development of Th0 and Th2 subsets, but not for the maintenance of cytokine production.     

Frequency domain modeling of reradiation in highly scattering media

We present a straightforward procedure for frequency domain modeling of reradiation in a highly scattering medium with an arbitrary, finite three-dimensional geometry. We use a finite difference numerical solver to determine the fluence distribution at the excitation wavelength, which is then coupled to the emission wavelength with an array of equivalent reradiating sources. We then calculate the fluence distribution at the emission wavelength with a second, independent numerical simulation with new optical parameters appropriate to the emission wavelength, using the distributed reradiating sources as the excitation. We compare three-dimensional simulations of a fluorophore distributed in a scattering medium with experimental data. We also compare simulations of the Raman reradiation of small diamonds in a scattering medium with experiment.     

Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited fluorescence

We determined the group-delay dispersion (GDD) of five microscope objectives by measuring the second-order autocorrelation at the focal points of the objectives with two-photon excited fluorescence as the power square sensor. We found that typical microscope lens systems introduce significant GDD (2000-6500 fs(2)). The third-order dispersion determined for these objectives limits the minimum obtainable pulse width at the focal point of an objective to 20-30 fs if not compensated. No significant chromatic aberration or higher-order dispersion effects were found for any of the optical components measured within the wavelength range of 700-780 nm and for pulse widths greater than 50-60 fs.     

Novel materials in magnetic resonance imaging: high permittivity ceramics,metamaterials, metasurfaces and artificial dielectrics

This article reviews recent developments in designing and testing new types of materials which can be: (i) placed around the body for in vivo imaging, (ii) be integrated into a conventional RF coil, or (iii) form the resonator itself. These materials can improve the quality of MRI scans for both in vivo and magnetic resonance microscopy applications. The methodological section covers the basic operation and design of two different types of materials, namely high permittivity materials constructed from ceramics and artificial dielectrics/metasurfaces formed by coupled conductive subunits, either in air or surrounded by dielectric material. Applications of high permittivity materials and metasurfaces placed next to the body to neuroimaging and extremity imaging at 7 T, body and neuroimaging at 3 T, and extremity imaging at 1.5 T are shown. Results using ceramic resonators for both high field in vivo imaging and magnetic resonance microscopy are also shown. The development of new materials to improve MR image quality remains an active area of research, but has not yet found significant use in clinical applications. This is mainly due to practical issues such as specific absorption rate modelling, accurate and reproducible placement, and acceptable size/weight of such materials. The most successful area has been simple “dielectric pads” for neuroimaging at 7 T which were initially developed somewhat as a stop-gap while parallel transmit technology was being developed, but have continued to be used at many sites. Some of these issues can potentially be overcome using much lighter metasurfaces and artificial dielectrics, which are just beginning to be assessed.

     

Team VALOR's ESCHER: A Novel Electromechanical Biped for the DARPA Robotics Challenge

The Electric Series Compliant Humanoid for Emergency Response (ESCHER) platform represents the culmination of four years of development at Virginia Tech to produce a full‐sized force‐controlled humanoid robot capable of operating in unstructured environments. ESCHER's locomotion capability was demonstrated at the DARPA Robotics Challenge (DRC) Finals when it successfully navigated the 61 m loose dirt course. Team VALOR, a Track A team, developed ESCHER leveraging and improving upon bipedal humanoid technologies implemented in previous research efforts, specifically for traversing uneven terrain and sustained untethered operation. This paper presents the hardware platform, software, and control systems developed to field ESCHER at the DRC Finals. ESCHER's unique features include custom linear series elastic actuators in both single and dual actuator configurations and a whole‐body control framework supporting compliant locomotion across variable and shifting terrain. A high‐level software system designed using the robot operating system integrated various open‐source packages and interfaced with the existing whole‐body motion controller. The paper discusses a detailed analysis of challenges encountered during the competition, along with lessons learned that are critical for transitioning research contributions to a fielded robot. Empirical data collected before, during, and after the DRC Finals validate ESCHER's performance in fielded environments.     

A topometric system for wide area augmented reality

We describe a system designed to facilitate efficient communication of information relating to the physical world using augmented reality (AR). We bring together a range of technologies to create a system capable of operating in real-time, over wide areas and for both indoor and outdoor operations. The central concept is to integrate localised mapping and tracking based on real-time visual SLAM with global positioning from both GPS and indoor ultra-wide band (UWB) technology. The former allows accurate and repeatable creation and visualisation of AR annotations within local metric maps, whilst the latter provides a coarse global representation of the topology of the maps. We call this a ‘Topometric System’. The key elements are: robust and efficient vision based tracking and mapping using a Kalman filter framework; rapid and reliable vision based relocalisation of users within local maps; user interaction mechanisms for effective annotation insertion; and an integrated framework for managing and fusing mapping and positioning data. We present the results of experiments conducted over a wide area, with indoor and outdoor operations, which demonstrates successful creation and visualisation of large numbers of AR annotations over a range of different locations.     

Protein Surface Mimetics: Understanding How Ruthenium Tris(Bipyridines) Interact with Proteins

Protein surface mimetics achieve high‐affinity binding by exploiting a scaffold to project binding groups over a large area of solvent‐exposed protein surface to make multiple cooperative noncovalent interactions. Such recognition is a prerequisite for competitive/orthosteric inhibition of protein–protein interactions (PPIs). This paper describes biophysical and structural studies on ruthenium(II) tris(bipyridine) surface mimetics that recognize cytochrome (cyt) c and inhibit the cyt c/cyt c peroxidase (CCP) PPI. Binding is electrostatically driven, with enhanced affinity achieved through enthalpic contributions thought to arise from the ability of the surface mimetics to make a greater number of noncovalent interactions than CCP with surface‐exposed basic residues on cyt c. High‐field natural abundance ¹H,¹⁵N HSQC NMR experiments are consistent with surface mimetics binding to cyt c in similar manner to CCP. This provides a framework for understanding recognition of proteins by supramolecular receptors and informing the design of ligands superior to the protein partners upon which they are inspired.