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排序方式: 共有2611条查询结果,搜索用时 15 毫秒
51.
Murtas C Bruschi M Carnevali ML Petretto A Corradini E Prunotto M Candiano G degl'Innocenti ML Ghiggeri GM Allegri L 《Proteomics. Clinical applications》2011,5(1-2):90-97
Renal auto-immune diseases represent a major source of morbidity in humans. For many years the knowledge on mechanisms of auto-immunity involving the kidney has been uniquely based on animal models. However, these findings often could not be readily translated to humans owing to notably difference in antigen expression by human podocytes. One example is Heymann nephritis (HN), the experimental model of human membranous glomerulonephritis (MGN), which is obtained in rats by injecting antibodies against megalin, a protein that is not present in human glomeruli. Human studies could not be done in the past since sequencing required too much material exceeding what obtainable from tissue biopsies in vivo. Research is now on the way to identify auto-antigens and isolate specific auto-antibodies in humans. New technology developments based on tissue microdissection and proteomical analysis have facilitated the recent discoveries, allowing direct analysis of human tissue in vivo. Major advances on the pathogenesis of MGN, the prototype for the formation and glomerular deposition of auto-antibodies, are now in progress. Two independent groups have, in fact, demonstrated the existence of specific IgG(4) against phospholipase A2 receptor, aldose reductase and Mn-superoxide dismutase in glomerular eluates and in plasma of a prominent part of patients with MGN, suggesting a major role of these proteins as auto-antigens in human MGN. This review will focalize these aspects outlining the contribution of proteomics in most recent developments. 相似文献
52.
SNPs are positions of the DNA sequences where the differences among individuals are embedded. The knowledge of such SNPs is crucial for disease association studies, but even if the number of such positions is low (about 1% of the entire sequence), the cost to extract the complete information is actually very high. Recent studies have shown that DNA sequences are structured into blocks of positions, that are conserved during evolution, where there is strong correlation among values (alleles) of different loci. To reduce the cost of extracting SNPs information, the block structure of the DNA has suggested to limit the process to a subset of SNPs, the so-called Tag SNPs, that are able to maintain the most of the information contained in the whole sequence. In this paper, we apply a technique for feature selection based on integer programming to the problem of Tag SNP selection. Moreover, to test the quality of our approach, we consider also the problem of SNPs reconstruction, i.e. the problem of deriving unknown SNPs from the value of Tag SNPs and propose two reconstruction methods, one based on a majority vote and the other on a machine learning approach. We test our algorithm on two public data sets of different nature, providing results that are, when comparable, in line with the related literature. One of the interesting aspects of the proposed method is to be found in its capability to deal simultaneously with very large SNPs sets, and, in addition, to provide highly informative reconstruction rules in the form of logic formulas. 相似文献
53.
Pasquale De Meo Giovanni Quattrone Domenico Ursino 《User Modeling and User-Adapted Interaction》2010,20(1):41-86
In this paper we propose a query expansion and user profile enrichment approach to improve the performance of recommender
systems operating on a folksonomy, storing and classifying the tags used to label a set of available resources. Our approach
builds and maintains a profile for each user. When he submits a query (consisting of a set of tags) on this folksonomy to
retrieve a set of resources of his interest, it automatically finds further “authoritative” tags to enrich his query and proposes
them to him. All “authoritative” tags considered interesting by the user are exploited to refine his query and, along with
those tags directly specified by him, are stored in his profile in such a way to enrich it. The expansion of user queries
and the enrichment of user profiles allow any content-based recommender system operating on the folksonomy to retrieve and
suggest a high number of resources matching with user needs and desires. Moreover, enriched user profiles can guide any collaborative
filtering recommender system to proactively discover and suggest to a user many resources relevant to him, even if he has
not explicitly searched for them. 相似文献
54.
A new method, based on the maximum likelihood principle, through the numerical Expectation–Maximization algorithm, is proposed to estimate traffic matrices when traffic exhibits long-range dependence. The methods proposed so far in the literature do not account for long-range dependence. The method proposed in the present paper also provides an estimate of the Hurst parameter. Simulation results show that: (i) the estimate of the traffic matrix is more efficient than those obtained via existing techniques; (ii) the estimation error of the traffic matrix is lower for larger values of the true traffic intensity; (iii) the estimate of the Hurst parameter is slightly negatively biased. 相似文献
55.
Mauro Baluda Pietro Braione Giovanni Denaro Mauro Pezzè 《Software Quality Journal》2011,19(4):725-751
Structural code coverage criteria have been studied since the early seventies, and now they are well supported by commercial
and open-source tools and are commonly embedded in several advanced industrial processes. Most industrial applications still
refer to simple criteria, like statement and branch coverage, and consider complex criteria, like modified condition decision
coverage, only rarely and often driven by the requirements of certification agencies. The industrial value of structural criteria
is limited by the difficulty of achieving high coverage, due to both the complexity of deriving test cases that execute specific
uncovered elements and the presence of many infeasible elements in the code. In this paper, we propose a technique that both
generates test cases that execute yet uncovered branches and identifies infeasible branches that can be eliminated from the
computation of the branch coverage. In this way, we can increase branch coverage to closely approximate full coverage, thus
improving its industrial value. The algorithm combines symbolic analysis, abstraction refinement, and a novel technique named
coarsening, to execute unexplored branches, identify infeasible ones, and mitigate the state space explosion problem. In the paper,
we present the technique and illustrate its effectiveness through a set of experimental results obtained with a prototype
implementation. 相似文献
56.
Irene Taurino Sandro Carrara Mauro Giorcelli Alberto Tagliaferro Giovanni De MicheliAuthor vitae 《Sensors and actuators. B, Chemical》2011,160(1):327
In this study, we report on multi-walled carbon nanotubes fabricated on silicon substrate with four different orientations via chemical vapor deposition. It is well-known that chemical treatments improve the nanotube electrochemical reactivity by creating edge-like defects on their exposed sidewalls. Before use, we performed an acid treatment on carbon nanotubes. To prove the effect of the treatment on these nanostructured electrodes, contact angles were measured. Then, sensitivities and detection limits were evaluated performing cyclic voltammetry. Two target molecules were used: potassium ferricyanide, an inorganic electroactive molecule, and hydrogen peroxide that is a product of reactions catalyzed by many enzymes, such as oxidases and peroxidases. Carbon nanotubes with tilted tips become hydrophilic after the treatment showing a contact angle of 22° ± 2°. This kind of electrode has shown also the best electrochemical performance. Sensitivity and detection limit values are 110.0 ± 0.5 μA/(mM cm2) and 8 μM for potassium ferricyanide solutions and 16.4 ± 0.1 μA/(mM cm2) and 24 μM using hydrogen peroxide as target compound. Considering the results of wettability and voltammetric measurements, nanotubes with tilted tips-based electrodes are found to be the most promising for future biosensing applications. 相似文献
57.
In recent years, Grid systems and Peer to Peer networks are the most commonly-used solutions to achieve the same goal: the sharing of resources and services in heterogeneous, dynamic, distributed environments. Many studies have proposed hybrid approaches that try to conjugate the advantages of the two models. This paper proposes an architecture that integrates the P2P interaction model in Grid environments, so as to build an open cooperative model wherein Grid entities are composed in a decentralized way. In particular, this paper focuses on a QoS aware discovery algorithm for P2P Grid systems, analyzing protocol and explaining techniques used to improve its performance. 相似文献
58.
Gianclaudio Malgieri Giovanni Comandé 《Information & Communications Technology Law》2017,26(3):229-249
This article offers a new perspective on the boundaries between health and non-health data in the age of ‘Quantified-Self’ apps: the ‘data-sensitiveness-by-computational-distance’ approach-or, more simply, the ‘sensitive-by-distance’ approach. This approach takes into account two variables: the intrinsic sensitiveness (a static variable) of personal data and the computational distance (a dynamic variable) between some kinds of personal data and pure health (or sensitive) data, which depends upon computational capacity. From an objective perspective, computational capacity depends on the level of development of data retrieval technologies at a certain moment, the availability of ‘accessory data’, and the applicable legal restraints on processing data. From a subjective perspective, computational capacity depends on the specific data mining efforts (or the ability to invest in them) taken by a given data controller: economic resources, human resources, and the use of accessory data. A direct consequence of the expansion of augmented humanity in collecting and inferring personal data is the increasing loss of health data processing ‘legibility’ for data subjects. In order to address this issue, we propose exploiting the existing legal tools in the General Data Protection Regulation to empower data subjects (the right to data access, the right to know the logic involved in automated decision-making, data portability, etc.). 相似文献
59.
Francesco Marra Giovanni Poggi Carlo Sansone Luisa Verdoliva 《Multimedia Tools and Applications》2017,76(4):4765-4781
Camera model identification has great relevance for many forensic applications, and is receiving growing attention in the literature. Virtually all techniques rely on the traces left in the image by the long sequence of in-camera processes which are specific of each model. They differ in the prior assumptions, if any, and in how such evidence is gathered in expressive features. In this work we study a class of blind features, based on the analysis of the image residuals of all color bands. They are extracted locally, based on co-occurrence matrices of selected neighbors, and then used to train a classifier. A number of experiments are carried out on the well-known Dresden Image Database. Besides the full-knowledge case, where all models of interest are known in advance, other scenarios with more limited knowledge and partially corrupted images are also investigated. Experimental results show these features to provide a state-of-the-art performance. 相似文献
60.