CALMODULIN1 and WRKY53 Function in Plant Defense by Negatively Regulating the Jasmonic Acid Biosynthesis Pathway in Arabidopsis

Jasmonic acid (JA) is an important hormone that functions in plant defense. cam1 and wrky53 mutants were more resistant to Spodoptera littoralis than in the wild-type (WT) Arabidopsis group. In addition, JA concentration in cam1 and wrky53 mutants was higher compared with the WT group. To explore how these two proteins affect the resistance of Arabidopsis plants, we used a yeast two-hybrid assay, firefly luciferase complementation imaging assay and in vitro pull-down assay confirming that calmodulin 1 (CAM1) interacted with WRKY53. However, these two proteins separate when calcium concentration increases in Arabidopsis leaf cells. Then, electrophoretic mobility shift assay and luciferase activation assay were used to verify that WRKY53 could bind to lipoxygenases 3 (LOX3) and lipoxygenases 4 (LOX4) gene promoters and negatively regulate gene expression. This study reveals that CAM1 and WRKY53 negatively regulate plant resistance to herbivory by regulating the JA biosynthesis pathway via the dissociation of CAM1-WRKY53, then the released WRKY53 binds to the LOXs promoters to negatively regulate LOXs gene expression. This study reveals WRKY53′s mechanism in insect resistance, a new light on the function of WRKY53.     

根据原油成熟度参数确定排烃门限的新方法——基于渤海海域莱州湾凹陷KL6D构造区烃源岩评价与油源对比

针对渤海海域莱州湾凹陷钻井少、有机地球化学资料不够丰富的特点,基于KL6D构造区烃源岩评价与油源对比,提出了一种根据原油甾烷异构化成熟度参数C29ααα20S/(20S+20R)确定排烃门限的新方法。根据KL6D构造区烃源岩和已发现原油的C29ααα20S/(20S+20R)成熟度参数与深度拟合的线性公式,确定该构造及围区的排烃门限深度为3 320 m,该深度以下烃源岩对研究区油气成藏有显著贡献。本文方法确定的排烃门限能直观地刻画研究区有效烃源岩,特别适用于少井和浅井地区。     

Topset-to-forest rollover trajectories as reliable predictors of sediment-volume partitioning into deep-lake areas

Topset-to-forest rollover trajectories and their relation to sediment-and sand-budget partitioning into deep-lake areas are far from being well understood,as co...     

基于改进BP算法的网络态势预测模型

准确的网络态势预测可以有效的减小网络拥塞程度.本文对网络拓扑和流量数据进行分析,提取了节点数、OD流数、大象流数、总流量等反应网络态势的参数,建立了基于BP神经网络算法的预测模型进行模拟,能够有效的预测上述参数的发展趋势,在网络管理中有较好的应用前景.     

Improving Lipid Production of Yarrowia lipolytica by the Aldehyde Dehydrogenase-Mediated Furfural Detoxification

Yarrowia lipolytica, the non-conventional yeast capable of high lipogenesis, is a microbial chassis for producing lipid-based biofuels and chemicals from renewable resources such as lignocellulosic biomass. However, the low tolerance of Y. lipolytica against furfural, a major inhibitory furan aldehyde derived from the pretreatment processes of lignocellulosic biomass, has restricted the efficient conversion of lignocellulosic hydrolysates. In this study, the furfural tolerance of Y. lipolytica has been improved by supporting its endogenous detoxification mechanism. Specifically, the endogenous genes encoding the aldehyde dehydrogenase family proteins were overexpressed in Y. lipolytica to support the conversion of furfural to furoic acid. Among them, YALI0E15400p (FALDH2) has shown the highest conversion rate of furfural to furoic acid and resulted in two-fold increased cell growth and lipid production in the presence of 0.4 g/L of furfural. To our knowledge, this is the first report to identify the native furfural detoxification mechanism and increase furfural resistance through rational engineering in Y. lipolytica. Overall, these results will improve the potential of Y. lipolytica to produce lipids and other value-added chemicals from a carbon-neutral feedstock of lignocellulosic biomass.     

New global synchronization analysis for complex networks with coupling delay based on a useful inequality

This paper is concerned with the problem of global synchronization for a general complex networks. Based on a useful inequality and Kronecker product technique, a new criterion is obtained, which has fewer unknown variables and is a significant improvement in the performance. Synchronization criteria are derived by some new mathematical skills and Schur complement. The result is expressed by linear matrix inequalities, which can be easily computed and checked in practice. Finally, numerical examples will be used to show the effectiveness of the obtained result.     

The microstructure and tensile property degradation of a gamma-TiAl alloy during isothermal and cyclic high temperature exposures

The microstructure and tensile property changes of a γTiAl-based alloy, Ti-47Al-2Cr-2Nb-0.15B, during isothermal and cyclic high temperature exposures have been investigated. Microstructure coarsening, including the fracture, dissolution and spheroidization of α₂ lamellae, is considered to be the main cause of the evident tensile property decreases of the alloy after isothermal and cyclic high temperature exposures. Dislocation emission near the broken lamellae is also an important phenomenon in the microstructure degradation during cyclic high temperature exposure, which originated from the thermal mismatch between α₂ and γ phases.     

折弯机矩形复合导轨系统

在分析折弯机滑块几种导轨系统的基础上,提出了矩形复合导轨系统。该系统能够随着滑块的倾斜而旋转,实现油缸运行方向和工作台长度方向的导向,同时将卸载时的振动冲击降到最小,并消除滑块的侧偏移。通过实践使用验证,得出矩形复合导轨系统是折弯机滑块导轨系统中理想的导向方式的结论。     

A Low-Complexity Precoding Scheme for Downlink Massive MU-MIMO Systems with Low-Resolution DACs

In this paper, we investigate the problem of downlink precoding for the narrowband massive multi-user multiple-input multiple-output (MU-MIMO) system with low-resolution digital-to-analog converters (DACs). We introduce a low-complexity precoding scheme based on the alternating direction method of multipliers (ADMM) framework in this work. An efficient gradient descent (GD) algorithm with adaptive step-size determination mechanism (ASGD) is proposed to alleviate the computational complexity bottleneck of the inherent matrix inversion. Numerical results demonstrate that the ASGD precoder achieves an attractive trade-off between the performance and computational complexity compared with other counterparts.