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31.
Valeska Cid-Jofr Macarena Moreno Ramn Sotomayor-Zrate Gonzalo Cruz Georgina M. Renard 《International journal of molecular sciences》2022,23(12)
The misuse of psychostimulants is an increasing behavior among young people, highlighting in some countries the abuse of modafinil (MOD) as a neuropotentiator. However, several clinical trials are investigating MOD as an alternative pharmacological treatment for attentional deficit and hyperactivity disorder (ADHD) in children and adolescents. On the other hand, the early use of psychostimulants and the misdiagnosis rates in ADHD make it crucial to investigate the brain effects of this type of drug in young healthy individuals. The aim of this work was to evaluate the effects of chronic MOD treatment on neurochemicals (γ-aminobutyric acid and glutamate), dopamine receptor 2 (D2) expression and behavior (non-selective attention “NSA”) in the mesocorticolimbic system of young healthy Sprague–Dawley rats. Preadolescent male rats were injected with MOD (75 mg/kg, i.p.) or a vehicle for 14 days (from postnatal day 22 to 35). At postnatal day 36, we measured the GLU and GABA contents and their extracellular levels in the nucleus accumbens (NAc). In addition, the GLU and GABA contents were measured in the ventral tegmental area (VTA) and D2 protein levels in the prefrontal cortex (PFC). Chronic use of MOD during adolescence induces behavioral and neurochemical changes associated with the mesocorticolimbic system, such as a reduction in PFC D2 expression, VTA GABA levels and NSA. These results contribute to the understanding of the neurological effects of chronic MOD use on a young healthy brain. 相似文献
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Jorge Barrios Alain Piétrus Gonzalo Joya Aymée Marrero Héctor de Arazoza 《Soft Computing - A Fusion of Foundations, Methodologies and Applications》2013,17(2):239-253
In this paper we deal with the application of differential inclusions to modeling nonlinear dynamical systems under uncertainty in parameters. In this case, differential inclusions seem to be better suited to modeling practical situations under uncertainty and imprecision than formulations by means of fuzzy differential equations. We develop a practical algorithm to approximate the reachable sets of a class of nonlinear differential inclusion, which eludes the computational problems of a previous set-valued version of the Heun’s method. Our algorithm is based on a complete discretization (time and state space) of the differential inclusion and it suits hardware features, handling the memory used by the method in a controlled fashion during all iterations. As a case of study, we formulate a differential inclusion to model an epidemic outbreak of dengue fever under Cuban conditions. The model takes into account interaction of human and mosquito populations as well as vertical transmission in the mosquito population. It is studied from the theoretical point of view to apply the Practical Algorithm. Also, we estimate the temporal evolution of the different human and mosquito populations given by the model in the Dengue 3 epidemic in Havana 2001, through the computation of the reachable sets using the Practical Algorithm. 相似文献
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Ikonen P.M.T. Saenz E. Gonzalo R. Tretyakov S.A. 《Antennas and Propagation, IEEE Transactions on》2007,55(10):2692-2700
We study the characteristics and radiation mechanism of antenna superstrates based on closely located periodical grids of loaded wires. An explicit analytical method based on the local field approach is used to study the reflection and transmission properties of such superstrates. It is shown that as a result of proper impedance loading there exists a rather wide frequency band over which currents induced to the grids cancel each other, leading to a wide transmission maximum. In this regime radiation is produced by the magnetic dipole moments created by circulating out-of-phase currents flowing in the grids. An impedance matrix representation is derived for the superstrates, and the analytical results are validated using full-wave simulations. As a practical application example we study numerically the radiation characteristics of dipole antennas illuminating finite-size superstrates. 相似文献
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38.
Use of β-hydroxyacyl-CoA-dehydrogenase (HADH) activity to differentiate frozen from unfrozen fish and shellfish 总被引:1,自引:0,他引:1
Manuela Fernández Sergio Mano Gonzalo D. García de Fernando Juan A. Ordóñez L. Hoz 《European Food Research and Technology》1999,209(3-4):205-208
Further work on an enzymic method to differentiate frozen from unfrozen fish and shellfish is reported. The method is based
on the release of the β-hydroxyacyl-CoA-dehydrogenase (HADH) from mitochondria during freezing. Enzymic activity was evaluated
in fresh and frozen thawed samples from sole (Solea solea), sea bream (Pagellus centrodontus), hake (Merluccius merluccius), gilt headed bream (Sparus aurata), sea bass (Dicentrarchus labrax), salmon (Salmo salar), prawn (Penaeus japonicus) and Norwegian lobster (Nephrops norvegicus). Changes in the HADH activity of fresh and frozen thawed samples were compared after freezing at –196 °C for 15 min. Two
values were obtained: U (by dividing: HADH activity of samples frozen at –196 °C, then thawed/HADH activity of unfrozen samples) and F (by dividing: HADH activity of samples frozen at –18 °C, thawed, then frozen at –196 °C /HADH activity of samples frozen
at –18 °C, then thawed). Statistical analysis showed significant differences (P≤0.05) between both quotients for gilt headed bream, salmon, sea bream, sole and prawn, and an arbitrary limit was set at
2 to differentiate frozen thawed from unfrozen samples. The application of this limit made it possible to discriminate the
unfrozen from the frozen thawed state of around 90% of the total samples analysed. Best results were obtained for prawn (100%
of samples differentiated). In the present paper, a laboratory routine is proposed based on the comparison of the HADH activity
of a sample analysed straight away and that of a sample frozen at –196 °C and then thawed. The reported method is simple
and fast. The entire laboratory procedure can be performed in 45 min.
Received: 20 July 1998 / Revised version: 2 November 1998 相似文献
39.
Carmen Coya Constanza Ruiz Ángel Luis Álvarez Susana Álvarez-García Eva M. García-Frutos Berta Gómez-Lor Alicia de Andrés 《Organic Electronics》2012,13(10):2138-2148
We present a series of differently substituted star-shaped hexaaryltriindoles with tunable light-emitting properties. The deep blue emission is unchanged by donor peripheral substituents while an increasing acceptor character produces a reduction of the optical gap, an increased Stokes shift and eventually leads to the appearance of a new electronic level and to the simultaneous deep blue (413 nm) and green (552 nm) emission in solution. Quenching by concentration increases with the acceptor character but is lower as the tendency of these compounds to aggregate is stronger. Solution processed thin films present optical and morphological qualities adequate for device fabrication and similar electronic structure compared to solutions with an emission range from 423 nm up to 657 nm (red), demonstrating the possibility of tuning the energy levels by chemical functionalization. We have fabricated and characterized single-layer solution processed organic light emitting diodes (OLED) to investigate the influence on transport and emission properties of the substituting species. We analyzed the I–V response using a single-carrier numerical model that includes injection barriers and non-uniform electric-field across the layer. As a result, we obtained the electric field dependence of the mobility for each device. Best results are obtained on the most electron rich derivative functionalized with six donor methoxy groups. This material shows the highest emission efficiency in solid state, due to aggregation-induced enhancement, and better transport properties with the highest mobility and a very low turn-on voltage of 2.8 V. The solution processed OLED devices produce stable deep blue (CIE coordinates (0.16, 0.16)) to white (CIE coordinates (0.33, 0.3)) emission with similar luminous efficiencies. 相似文献
40.
Alexander Pavlov Gonzalo D Garcia De Fernando Juan A Ordoez Lorenzo Hoz 《Journal of the science of food and agriculture》1994,64(2):141-143
The β-hydroxyacyl-CoA-dehydrogenase (HADH) activity of unfrozen and thawed frog legs was investigated. The enzyme was extracted by either immersing frog legs in phosphate buffer 0.1 M, pH 6.0 at 25°C for 15 min or pressing them between trichinoscopy glasses. The enzyme activity was assayed using acetoacetyl-CoA as substrate and measured spectrophotometrically at 340 nm. It was possible by both extraction methods to distinguish between thawed and unfrozen samples although when the juice was obtained by pressing the HADH activity of the dilution was ~ 1.5 times higher than that obtained by immersion. The HADH activity was significantly higher (P≤0·001) in frozen-thawed frogs than in unfrozen legs because during freezing there is a release of HADH. No significative differences were found in the HADH activity in samples frozen in the temperature range -10 to -196°C. HADH activity was not affected by the storage time in crushed ice up to 6 days. 相似文献