Influence of chlorhexidine on dentin adhesive interface micromorphology and nanoleakage expression of resin cements

This study focused on adhesive interface morphologic characterization and nanoleakage expression of resin cements bonded to human dentin pretreated with 1% chlorhexidine (CHX). Thirty‐two non‐carious human third molars were ground flat to expose superficial dentin. Resin composite blocks were luted to the exposed dentin using one conventional (RelyX ARC) and one self‐adhesive resin cement (RelyX U100), with/without CHX pretreatment. Four groups (n = 8) were obtained: control groups (ARC and U100); experimental groups (ARC/CHX and U100/CHX) were pretreated with 1% CHX prior to the luting process. After storage in water for 24 h, the bonded teeth were sectioned into 0.9 × 0.9 mm² sticks producing a minimum of 12 sticks per tooth. Four sticks from each tooth were prepared for hybrid layer evaluation by scanning electron microscope analysis. The remaining sticks were immersed in silver nitrate for 24 h for either nanoleakage evaluation along the bonded interfaces or after rupture. Nanoleakage samples were carbon coated and examined using backscattered electron mode. Well‐established hybrid layers were observed in the groups luted with RelyX ARC. Nanoleakage evaluation revealed increase nanoleakage in groups treated with CHX for both resin cements. Group U100/CHX exhibited the most pronouncing nanoleakage expression along with porous zones adjacent to the CHX pretreated dentin. The results suggest a possible incompatibility between CHX and RelyX U100 that raises the concern that the use of CHX with self‐adhesive cements may adversely affect resin‐dentin bond. Microsc. Res. Tech. 76:788–794, 2013. © 2013 Wiley Periodicals, Inc.     

Antimicrobial, Antiproliferative and Proapoptotic Activities of Extract, Fractions and Isolated Compounds from the Stem of Erythroxylum caatingae Plowman

In the study, we have examined the antitumor and antimicrobial activities of the methanol extract, the fractions, a fraction of total alkaloids and two alkaloids isolated from the stem of Erythroxylum caatingae Plowman. All test fractions, except the hexane fractions, showed antimicrobial activity on gram-positive bacteria and fungi. The acetate: methanol (95:5), acetate, chloroform and hexane fractions show the highest cytotoxicity activity against the NCI-H292, HEp-2 and K562 cell lines using MTT. The absence of hemolysis in the erythrocytes of mice was observed in these fractions and 6β-Benzoyloxy-3α-(3,4,5- trimethoxybenzoyloxy) tropane (catuabine B). Staining with Annexin V-FITC and JC-1 was used to verify the mechanism of action of the compounds of E. caatingae that showed cytotoxicity less than 30 μg/mL in leukemic cells. After 48 h of incubation, we observed that the acetate: methanol (95:5), acetate, and chloroform fractions, as well as the catuabine B, increased in the number of cells in early apoptosis, from 53.0 to 74.8%. An analysis of the potential of the mitochondrial membrane by incorporation of JC-1 showed that most cells during incubation of the acetate: methanol (95:5) and acetate fractions (63.85 and 59.2%) were stained, suggesting the involvement of an intrinsic pathway of apoptosis.     

Monitoring pesticide residues in greenhouse tomato by combining acetonitrile-based extraction with dispersive liquid-liquid microextraction followed by gas-chromatography-mass spectrometry

A multiclass and multiresidue method for pesticide analysis in tomato was validated. Extraction and pre-concentration of the pesticide residues from acetonitrile extracts was performed by using dispersive liquid-liquid microextraction (DLLME) technique, followed by gas chromatography-mass detection. DLLME was performed using carbon tetrachloride as extractive solvent and acetonitrile extract as dispersive solvent, in order to increase enrichment factor of the extraction procedure. Validation parameters indicated the suitability of the method for routine analyses of thirty pesticides in a large number of samples. In general, pesticide recoveries ranged between 70% and 110% and repeatability ranged between 1% and 20%. The proposed method was applied to the monitoring of pesticides in tomatoes grown during winter in greenhouses. Among the compounds considered in this work, cyprodinil was found in tomato at concentrations of 0.33mg/kg, other pesticides like azoxystrobin, fenhexanid, tolyfluanid, λ-cyhalothrin and trifloxystrobin were also detected, but, not quantified.     

Eight-and-a-half-month-old infants' reasoning about containment events

The present research examined whether 8.5-month-old infants take into account the width and compressibility of an object when determining whether it can be inserted into a container. The infants in Experiment 1 saw 2 test events. At the start of each event, a tall container rested on the apparatus floor. Next, the container was hidden by a screen, and a large ball attached to the lower end of a rod was introduced into the apparatus and lowered behind the screen into the container. Finally, the screen was removed to reveal the ball's rod protruding above the container's rim. The only difference between the 2 test events had to do with the width of the containers: in 1 event (large-container event), the container was slightly wider than the ball; in the other event (small-container event), the container was only half as wide as the ball, so that it should have been impossible for the ball to be lowered into it. Infants in a control condition saw identical test events except that a small ball was used that could fit into either the large or the small container. The infants in the experimental condition looked reliably longer at the small- than at the large-container event, whereas those in the control condition tended to look equally at the 2 events. These results suggested that, although the infants never saw the ball and the container simultaneously, they realized that the large ball could fit into the large but not the small container, whereas the small ball could fit into both containers. In Experiment 2, the large ball used in Experiment 1 was replaced with an equally large but compressible ball. The results were negative, suggesting that the infants understood that the large compressible ball could be inserted into either the small or the large container. Finally, Experiment 3 confirmed the results of the experimental condition in Experiment 1, with a slightly different procedure. Together, the present results indicate that, by 8.5 months of age, infants are already capable of sophisticated reasoning about containment events.     

Solid-state 13C nuclear magnetic resonance spectra of 6-aminopenicillanic acid

Factors which affected the severity of scoliosis induced by rib resection were examined. Seventy chickens were divided into a rib transection group and a rib resection group with seven subgroups, according to the number of ribs treated and the age at surgery, and examined radiographically until 20 weeks postoperatively. Rib transection group chickens showed fusion at the transected site and no marked scoliosis. In the rib resection group, the more ribs resected and the younger the age at operation, the more severely the scoliosis developed. Bone regeneration at the resection site made the progression of scoliosis milder. These findings indicate that the age at rib resection, the number of ribs that are operated on, and nonunion of the resected sites play important roles in the progression of scoliosis induced by rib resection.     

Misalignment problem in matrix decomposition with missing values

Data collection within a real-world environment may be compromised by several factors such as data-logger malfunctions and communication errors, during which no data is collected. As a consequence, appropriate tools are required to handle the missing values when analysing and processing such data. This problem is often tackled via matrix decomposition. While it has been successfully applied in a wide range of applications, in this work we report an issue that has been neglected in literature and “degenerates” the quality of the imputations obtained by matrix decomposition in multivariate time-series (with smooth evolution). Briefly, the problem consists of the misalignment of the matrix decomposition result: the missing values imputations fall within an incorrect range of values and the transitions between observed and imputed values are not smooth. We address this problem by proposing a post-processing alignment strategy. According to our experiments, the post-processing adjustment substantially improves the accuracy of the imputations (when the misalignment occurs). Moreover, the results also suggest that the misalignment occurs mostly when dealing with a small number of time-series due to lack of generalization ability.

     

A Computational Geometry Approach for Localization and Tracking in GPS‐denied Environments*

Localization and tracking of vehicles is still an important issue in GPS‐denied environments (both indoors and outdoors), where accurate motion is required. In this work, a localization system based on the random disposition of LiDAR sensors (which share a partially common field of view) and on the use of the Hausdorff distance is addressed. The proposed system uses the Hausdorff distance to estimate both the position of the LiDAR sensors and the pose of the vehicle as it drives within the environment. Our approach is not restricted to the number of LiDAR sensors (the estimation procedure is asynchronous), the number of vehicles (it is a multidimensional approach), or the nature of the environment. However, it is implemented in open spaces, limited by the range of the LiDAR sensors and the geometry of the vehicle. An empirical analysis of the presented approach is also included here, showing that the error in the localization estimation remains bounded in approximately 50 cm. Real‐time experimentation as validation of the proposed localization and tracking techniques as well as the pros and cons of our proposal are also shown in this work.     

Spectral separability of riparian forests from small and medium-sized rivers across a latitudinal gradient using multispectral imagery

Spectral discrimination between riparian forests is a challenging issue due to the inherent complexity of species composition and the high spatial structural variability of these vegetation types. This study aimed to evaluate spectral separability among riparian forests, in small and medium-sized river catchment areas, in three bioclimatic zones of Portugal (temperate, transitional, and Mediterranean). We also assess the spectral differences using only the dominant riparian woody species in each riparian forest class, namely Alnus glutinosa, Salix salviifolia, and Nerium oleander.

Pixel values were extracted from high-resolution airborne multispectral imagery (red, green, blue, and near-infrared, 50 cm pixels) of 26 riparian forests located in the three bioclimatic zones. Spectral separability was calculated using the transformed divergence (TD) distance. Discriminant analysis (DA) was used to select the bands that contribute most to the spectral separability and for the classification accuracy assessment of the riparian forests. Species composition and percentage of canopy closure were collected for all the riparian forests in a field campaign and subjected to hierarchical clustering in order to validate the spectral separability analyses. Optical traits derived from field data were used to interpret the spectral differences between riparian forest classes.

The greatest spectral separability was observed between the temperate and the Mediterranean riparian forest classes. Global classification accuracy for the DA was 86.3% for riparian forest classes along medium-sized rivers and 70.1% in small-sized ones. The high floristic and spatial structure variability was responsible for the misclassification errors that occurred between the transitional and the other riparian forest classes. The spectral separability using only the dominant species was greater than that obtained using the overall species assemblages of the riparian forests. Alnus glutinosa had the highest level of classification accuracy, and this may be related to its peculiar yellowish-green tone. DA also revealed that all spectral bands were needed in order to distinguish the riparian forest classes.

This study provided evidence that the spectral discrimination of riparian forests can be explained on the basis of differences in species composition and cover, and by a convergence of optical traits, at both leaf and canopy levels. Spectral signatures of these riparian forests and related spectral signatures of key species are useful tools for evaluating the floristic deviations of actual riparian forests from their near-natural benchmarks.

     

MobiLine: A Nested Software Product Line for the domain of mobile and context-aware applications

Mobile devices are multipurpose and multi-sensor equipments supporting applications able to adapt their behavior according to changes in the user’s context (device, location, time, etc.). Meanwhile, the development of mobile and context-aware software is not a simple task, mostly due to the peculiar characteristics of these devices. Although several solutions have been proposed to facilitate their development, reuse is not systematically used throughout the software development life-cycle. In this paper, we discuss an approach for the development of mobile and context-aware software using the Software Product Line (SPL) paradigm. Furthermore, a Nested SPL for the domain of mobile and context-aware applications is presented, lessons learned in the SPL development are discussed and a product for a context-aware visit guide is shown.     

Cellulose exopolysaccharide from sugarcane molasses as a suitable substrate for 2D and 3D neuron and astrocyte primary cultures

Bacteria-synthesized polysaccharides have attracted interest for biomedical applications as promising biomaterials to be used as implants and scaffolds. The present study tested the hypothesis that cellulose exopolysaccharide (CEC) produced from sugarcane molasses of low cost and adequate purity would be suitable as a template for 2D and 3D neuron and/or astrocyte primary cultures, considering its low toxicity. CEC biocompatibility in these primary cultures was evaluated with respect to cell viability, adhesion, growth and cell function (calcium imaging). Polystyrene or Matrigel® matrix were used as comparative controls. We demonstrated that the properties of this CEC in the 2D or 3D configurations are suitable for differentiation of cortical astrocytes and neurons in single or mixed cultures. No toxicity was detected in neurons that showed NMDA-induced Ca²⁺ influx. Unlike other polysaccharides of bacterial synthesis, the CEC was efficient as a support even in the absence of surface conjugation with extracellular matrix proteins, maintaining physiological characteristics of cultured neural cells. These observations open up the perspective for development of a novel 3D biofunctional scaffold produced from bacterial cellulose and obtained from renewable sources whose residues are not pollutants. Its low cost and possibility to be manufactured in scale are also suitable for potential applications in regenerative medicine.

Open image in new window