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Shengchen Gu Xupeng Zang Lei Jiang Ting Gu Fanming Meng Sixiu Huang Gengyuan Cai Zicong Li Zhenfang Wu Linjun Hong 《International journal of molecular sciences》2022,23(15)
Litter size is an important indicator to measure the production capacity of commercial pigs. Spontaneous embryo loss is an essential factor in determining sow litter size. In early pregnancy, spontaneous embryo loss in porcine is as high as 20–30% during embryo implantation. However, the specific molecular mechanism underlying spontaneous embryo loss at the end of embryo implantation remains unknown. Therefore, we comprehensively used small RNA sequencing technology, bioinformatics analysis, and molecular experiments to determine the microRNA (miRNA) expression profile in the healthy and arresting embryo implantation site of porcine endometrium on day of gestation (DG) 28. A total of 464 miRNAs were identified in arresting endometrium (AE) and healthy endometrium (HE), and 139 differentially expressed miRNAs (DEMs) were screened. We combined the mRNA sequencing dataset from the SRA database to predict the target genes of these miRNAs. A quantitative real-time PCR assay identified the expression levels of miRNAs and mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed target genes of DEMs, mainly enriched in epithelial development and amino acids metabolism-related pathways. We performed fluorescence in situ hybridization (FISH) and the dual-luciferase report gene assay to confirm miRNA and predicted target gene binding. miR-205 may inhibit its expression by combining 3′-untranslated regions (3′ UTR) of tubulointerstitial nephritis antigen-like 1 (TINAGL1). The resulting inhibition of angiogenesis in the maternal endometrium ultimately leads to the formation of arresting embryos during the implantation period. This study provides a reference for the effect of miRNA on the successful implantation of pig embryos in early gestation. 相似文献
84.
Multiplexed Bioassays: Dual‐Encoded Microbeads through a Host–Guest Structure: Enormous,Flexible, and Accurate Barcodes for Multiplexed Assays (Adv. Funct. Mater. 34/2016) 下载免费PDF全文
85.
移动IPv6安全绑定更新机制研究 总被引:1,自引:0,他引:1
介绍了移动IPv6网络中节点移动后进行三角路由优化所引出的绑定更新安全问题,阐述了在非PKI体系中解决绑定更新安全隐患的几种机制;在阐述的同时对这些机制进行了安全性分析,最后对这几种机制从安全性角度进行比较. 相似文献
86.
Joonwon Bae Gyo Eun Gu Yeon Ju Kwon Jea Uk Lee Jin-Yong Hong 《International journal of molecular sciences》2022,23(9)
As the control over radioactive species becomes critical for the contemporary human life, the development of functional materials for decontamination of radioactive substances has also become important. In this work, a three-dimensional (3D) porous carbon monolith functionalized with Prussian blue particles was prepared through removal of colloidal silica particles from exfoliated graphene/silica composite precursors. The colloidal silica particles with a narrow size distribution were used to act a role of hard template and provide a sufficient surface area that could accommodate potentially hazardous radioactive substances by adsorption. The unique surface and pore structure of the functionalized porous carbon monolith was examined using electron microscopy and energy-dispersive X-ray analysis (EDS). The effective incorporation of PB nanoparticles was confirmed using diverse instrumentations such as X-ray diffraction (XRD), Fourier-transform infrared (FT-IR), and X-ray photoelectron spectroscopy (XPS). A nitrogen adsorption/desorption study showed that surface area and pore volume increased significantly compared with the starting precursor. Adsorption tests were performed with 133Cs ions to examine adsorption isotherms using both Langmuir and Freundlich isotherms. In addition, adsorption kinetics were also investigated and parameters were calculated. The functionalized porous carbon monolith showed a relatively higher adsorption capacity than that of pristine porous carbon monolith and the bulk PB to most radioactive ions such as 133Cs, 85Rb, 138Ba, 88Sr, 140Ce, and 205Tl. This material can be used for decontamination in expanded application fields. 相似文献
87.
在行人重识别任务中存在数据集标注难度大,样本量少,特征提取后细节特征缺失等问题。针对以上问题提出深度双重注意力的生成与判别联合学习的行人重识别。首先,构建联合学习框架,将判别模块嵌入生成模块,实现图像生成和判别端到端的训练,及时将生成图像反馈给判别模块,同时优化生成模块与判别模块。其次,通过相邻的通道注意力模块间连接和相邻空间注意力模块间连接,融合所有通道特征和空间特征,构建深度双重注意力模块,将其嵌入教师模型,使模型能更好地提取行人细节身份特征,提高模型识别能力。实验结果表明,该算法在Market-1501和DukeMTMC-ReID数据集上具有较好的鲁棒性、判别性。 相似文献
88.
浅谈课堂教学中的德育渗透 总被引:1,自引:0,他引:1
德育教育是全体教师的共同职责,本文从课堂德育教育缺失的原因分析出发,紧紧抓住课堂德育渗透这一重要渠道,阐述了德育渗透的重要性,并结合实际提出了课堂教学中德育渗透的基本途径。 相似文献
89.
R. Gul A. Bolotnikov H. K. Kim R. Rodriguez K. Keeter Z. Li G. Gu R. B. James 《Journal of Electronic Materials》2011,40(3):274-279
We studied, by current deep-level transient spectroscopy (I-DLTS), point defects in CdZnTe detectors grown by different techniques. We identified 12 different traps with energy levels from 7 meV to 1.1 eV. Although the levels of most of the identified defects were independent of the crystal growth techniques, nevertheless there were some associated differences in the traps’ energies and densities. 相似文献
90.
三十年前我国的网印技术是落后的,工作者多数是手工作坊式的生产,沿用着传统的刻膜技术和简单的明胶大漆制版方法制作一般的网印版,印制产量低、质量不高。 相似文献