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41.
An inhibition ELISA (IH-ELISA) test for foot-and-mouth disease virus (FMDV) was validated using 106 epithelial samples from suspected cases of FMD in Argentina submitted to the Argentine National Diagnostics Laboratory (GELAB) over a period of 12 months and examined in parallel with the complement fixation test (CFT). IH-ELISA was found to be more sensitive, detecting 25% (26 samples) more FMDV positives than the CFT in original suspensions of field samples. The effect of storage conditions on 12S stability was examined. Plates stored at 4 degrees C blocked with 1% ovalbumin and plates stored at -20 degrees C with or without blocking buffer could be used for at least 90 days. When various brands of polystyrene plates were compared for 12ps adsorption it was found that those microplates of higher binding capacity were more efficient. 相似文献
42.
Comparisons of Urban Travel Forecasts Prepared with the Sequential Procedure and a Combined Model 总被引:4,自引:1,他引:3
Justin D. Siegel Joaquín De Cea José Enrique Fernández Renán E. Rodriguez David Boyce 《Networks and Spatial Economics》2006,6(2):135-148
Detailed analyses and comparisons of urban travel forecasts prepared by applying the state-of-practice sequential procedure
and the solution of a combined network equilibrium model are presented. The sequential procedure for solving the trip distribution,
mode choice and assignment problems with feedback is the current practice in most transportation planning agencies, although
its important limitations are well known. The solution of a combined model, in contrast, results from a single mathematical
formulation, which ensures a well-converged and consistent result. Using a real network, several methods for solving the sequential
procedure with feedback are compared to the solution of the combined model ESTRAUS. The results of these methods are shown
to have various levels of instability. The paper concludes with a call for a new paradigm of travel forecasting practice based
on an internally consistent model formulation that can be solved to a level of precision suitable for comparing alternative
scenarios. 相似文献
43.
JR García Fernández MA montalbán ML Martín MC Ortiz MA Martínez A García Marcilla 《Canadian Metallurgical Quarterly》1998,43(5):430-432
Acute nonoliguric renal failure developed in a 13-year-old girl, 1 month after the institution of isoniazid therapy because of a positive tuberculin test at school screening. A renal biopsy demonstrated severe crescentic glomerulonephritis with focal interstitial changes. Discontinuation of isoniazid and a short course of steroids and cyclophosphamide therapy were followed by complete recovery. Whereas isoniazid has been shown to induce a lupus-like syndrome and antihistone antinuclear antibodies, our patient displayed none of the clinical or immunological features that are characteristic of drug-induced lupus. Furthermore, none of the identifiable causes for crescentic glomerulonephritis was evident in this girl. To the best of our knowledge this is the first report suggesting a possible association of crescentic glomerulonephritis to isoniazid treatment. 相似文献
44.
45.
JC López Pacios L Parra Muntaner MC Pi?eiro Fernández SC Gómez Cisneros E Sánchez Sánchez JA Rivas Escudero FJ Madrid García J García Alonso 《Canadian Metallurgical Quarterly》1998,51(4):331-333
The occurrence of secondary hypogonadism is a common finding in males who seek help with erectile dysfunction, although the relationship to diminished testosterone is unclear. Two possibilities exist regarding both the genesis and maintenance of the hypogonadotropic hypogonadal state. First, a defect in hypothalamic function, resulting in downregulation as well as in alterations of anterior pituitary function; second, estradiol inhibition of gonadotropin release, both of which result in decreased testosterone production. As testosterone levels decrease and estradiol levels increase, the ratio of free testosterone to estradiol reaches a critical point and the estrogenic gonadotropin suppressive effects predominate. This ratio may signal the biological point of no return and could become one of the criteria for defining the separation of the transitional hypogonadal state from the final 'end stage' hypogonadotropic hypogonadal state. As the aging process continues, there is a relative accumulation of fatty tissue, and aromatization accelerates the conversion of testosterone to estradiol. This additional secondary estradiol inhibition results in the maintenance of the testosterone deficient state, and the aging process continues uncontested. 相似文献
46.
P Neumann JE Berglund E Fernández Mondéjar A Magnusson G Hedenstierna 《Canadian Metallurgical Quarterly》1998,85(4):1533-1543
Oleic acid (OA) injection, lung lavage, and endotoxin infusion are three commonly used methods to induce experimental lung injury. The dynamics of lung collapse and recruitment in these models have not been studied, although knowledge of this is desirable to establish ventilatory techniques that keep the lungs open. We measured lung density by computed tomography during breath-holding procedures. Lung injury was induced with OA, lung lavage, or endotoxin in groups of six mechanically ventilated pigs. After a stabilization period, repetitive computed tomography scans of the same slice were obtained during prolonged expirations with and without positive end-expiratory pressure and during prolonged inspirations after 5 and 30 s of expiration. Lung collapse and recruitment occurred mainly within the first 4 s of breath-holding procedures in all three lung injury models, and some collapse and recruitment occurred even within 0.6 s. OA-injured lungs were significantly more unstable than lungs injured by bronchoalveolar lavage or endotoxin infusion. In this experimental setting, expiration times <0.6 s are required to avoid cyclic alveolar collapse during mechanical ventilation without extrinsic positive end-expiratory pressure. 相似文献
47.
The Journal of Supercomputing - DNA methylation analysis has become an important topic in the study of human health. DNA methylation analysis requires not only a specific treatment of DNA samples... 相似文献
48.
Fernández Álvaro Fernández Camino Miguel-Dávila José-Ángel Conde Miguel Á. 《The Journal of supercomputing》2021,77(3):2302-2325
The Journal of Supercomputing - The integration of a Supercomputer in the educational process improves student’s technological skills. The aim of the paper is to study the interaction between... 相似文献
49.
Palomares Iván Martínez-Cámara Eugenio Montes Rosana García-Moral Pablo Chiachio Manuel Chiachio Juan Alonso Sergio Melero Francisco J. Molina Daniel Fernández Bárbara Moral Cristina Marchena Rosario de Vargas Javier Pérez Herrera Francisco 《Applied Intelligence》2021,51(9):6497-6527
Applied Intelligence - The17 Sustainable Development Goals (SDGs) established by the United Nations Agenda 2030 constitute a global blueprint agenda and instrument for peace and prosperity... 相似文献
50.
Jose Saldana Julián Fernández-Navajas José Ruiz-Mas Jenifer Murillo Eduardo Viruete Navarro José I. Aznar 《Computer Networks》2012,56(7):1893-1919
This work presents a study of RTP multiplexing schemes, which are compared with the normal use of RTP, in terms of experienced quality. Bandwidth saving, latency and packet loss for different options are studied, and some tests of Voice over IP (VoIP) traffic are carried out in order to compare the quality obtained using different implementations of the router buffer. Voice quality is calculated using ITU R-factor, which is a widely accepted quality estimator. The tests show the bandwidth savings of multiplexing, and also the importance of packet size for certain buffers, as latency and packet loss may be affected. The customer’s experience improvement is measured, showing that the use of multiplexing can be interesting in some scenarios, like an enterprise with different offices connected via the Internet. The system is also tested using different numbers of samples per packet, and the distribution of the flows into different tunnels is found to be an important factor in order to achieve an optimal perceived quality for each kind of buffer. Grouping all the flows into a single tunnel will not always be the best solution, as the increase of the number of flows does not improve bandwidth efficiency indefinitely. If the buffer penalizes big packets, it will be better to group the flows into a number of tunnels. The router processing capacity has to be taken into account too, as the limit of packets per second it can manage must not be exceeded. The obtained results show that multiplexing is a good way to improve customer’s experience of VoIP in scenarios where many RTP flows share the same path. 相似文献