Aggregated Scheduling of a Multiproduct Batch Plant by Two-Stage Stochastic Integer Programming

We consider the mid-term scheduling of a multiproduct batch plant. Market requirements for the final products are uncertain but given in terms of a probability distribution. We introduce a two-stage stochastic integer programming model for the problem and solve it by a scenario decomposition method based on Lagrangian relaxation. We describe heuristics and preprocessing for both the single- and the multi-scenario models. Preliminary computational results are presented.     

Simulation of batch and continuous reactors with co-immobilized yeast and β-galactosidase

A reaction-diffusion model was used to simulate a co-immobilized system utilizing the numerical method of orthogonal collocation. The production of ethanol from deproteinized whey using β-galactosidase co-immobilized with Saccharomyces cerevisiae in calcium alginate gel beads was chosen as a model system. Calculated concentrations of lactose, glucose, galactose and ethanol were compared with experimental data for a batch reactor and a continuous horizontal packed-bed reactor. The mathematical model has been used to analyse the influence of internal and external mass transfer for the continuous reactor. The external mass transfer was shown to be of minor importance. The introduction of baffles decreased the backmixing in the horizontal packed-bed reactor. Internal mass transfer was found to be the main cause of the reduction in the apparent reaction rate. Thus, much of the expected increase in reaction rate is diminished by mass transfer hindrance when the cell concentration is increased.     

Inhibition of nitric oxide synthesis aggravates reperfusion injury after hepatic ischemia and endotoxemia

The potential role of nitric oxide (NO) was investigated in the pathophysiology of liver injury after priming with 20 min hepatic ischemia-reperfusion and administration of .5 mg/kg Salmonella enteritidis endotoxin. Liver injury during the early reperfusion phase of 4 h was characterized by severe vascular oxidant stress, lipid peroxidation (LPO), neutrophil infiltration, and a 33% reduction of the microvascular blood flow in the liver. Inhibition of NO synthesis with N omega-nitro-L-arginine methyl ester hydrochloride (L-NAME) aggravated liver injury by 90%, reduced LPO, and did not affect liver neutrophils but further impaired microvascular blood flow. Treatment with the NO-donor spermine-NONOate or L-arginine did not affect these parameters in postischemic animals, however, treatment did restore all values of L-NAME-treated animals back to disease control levels. These data suggest that endogenous NO formation is sufficient to limit ischemic liver injury during reperfusion but inhibition of NO synthesis will result in additional ischemic damage. NO may also be involved in scavenging of superoxide in the vasculature and in inducing LPO.     

Analysis of low molecular weight plant phenolics by thermospray liquid chromatography/mass spectrometry

High performance liquid chromatography-mass spectrometry using a thermospray interface and discharge ionisation has been conducted on a group of plant phenolic standards and on a wheat straw acid hydrolysate. Protonated molecules, [M + H]⁺, were observed for the phenolic standards and it was possible to identify several phenolic compounds in the hydrolysate. These results indicate that thermospray HPLC/MS has potential as a technique for determining the presence of useful marker compounds which are indicative of the progress of the lignification during plant growth.     

Rough surface absorption of Nd:YAG laser rods

The rough surface of Nd:YAG-laser rods exhibits a broad spectral absorption due to impurities. The optical density of the investigated surfaces was determined up to several percent in the near infrared and visible spectral range. The characteristics of the rough surface absorption was investigated.     

Engineering camel single-domain antibodies and immobilization chemistry for human prostate-specific antigen sensing

The specificity and affinity characteristics of antibodies make them excellent probes in biosensor applications. Unfortunately, their large size, unstable behavior, and random immobilization properties create numerous problems. The single-domain antigen-binding fragment derived from heavy-chain antibodies of camelids (termed VHH) offers special advantages in terms of size, stability, and ease of generating different antibody constructs. In this study, we show the potential of those VHHs in sensing human prostate-specific antigen (hPSA) by SPR technology. Different VHH constructs were immobilized onto commercial and custom-built sensor surfaces by metal chelation, biotin-streptavidin interaction, or covalent coupling. The detection of subnanogram per milliliter hPSA concentrations could be attained on a covalently coupled three-dimensional dextran surface. Moreover, the ratio of different hPSA isoform concentrations could be assessed via a sandwich assay and resulted in the detection of clinically significant antigen concentrations within 15 min. In addition, for the first time, the intrinsic protein stability is presented as an important probe design factor, since our results reveal that higher intrinsic stability offers higher resistance to harsh regeneration conditions. In conclusion, we present VHHs as a novel class of biosensor probes rivaling conventional antibodies and their derived antibody fragments.     

Multi-VDD Testing for Analog Circuits

We present industrial results of a quiescent current testing technique suitable for RF testing. The operational method consists of ramping the power supply and of observing the corresponding quiescent current signatures. When the power supply is swept, all transistors are forced into various regions of operation. This has as advantage that the detection of faults is done for multiple supply voltages and corresponding quiescent currents, enhancing in this form the detectability of faults. We found that this method of structural testing yields fault coverage results comparable to functional RF tests making it a potential and attractive technique for production wafer testing due to its low cost, low testing times and low frequency requirements.José Pineda de Gyvez received the Ph.D. degree from the Eindhoven University of Technology. He is currently a principal scientist at Philips Research Laboratories, The Netherlands. Dr. Pineda was Associate Editor in IEEE Transactions on Circuits and Systems Part I and also Associate Editor for Technology in IEEE Transactions on Semiconductor Manufacturing. His research interests are in the general areas of design for manufacturability and analog signal processing.Guido Gronthoud received the electrical engineering degree from the Delft University in 1975. From 1976 to 1980 he worked at the Delft University on the design of Microwave systems. From 1980 he works with Philips. He has been working in the fields of circuit simulation and modelling for IC designs, CAD development for PCB design and electronic circuits and systems reliability. Since 1998 he is working on test innovation of digital and mixed-signal circuits. His interests are Defect Oriented Test, fault modeling and Process Related Test. He has authored and co-authored technical papers.