黄皮糯米酒的酿造工艺及香气成分的研究

研究了不同比例黄皮与糯米混合发酵黄皮糯米酒的工艺及风味改善方法。结果表明,黄皮汁与糯米比例为6∶4,糯米糖化48h后添加黄皮汁,并接入酿酒酵母在20℃下进行乙醇发酵,于成熟的酒中勾兑黄皮皮渣蒸馏酒,不但使成品酒突出了黄皮果酒清新淡雅的香气和糯米酒酒香醇厚特点,还对黄皮皮渣进行了有效利用。采用气质联用技术(GC/MS)分析黄皮糯米酒香气成分的结果表明,既含有黄皮主体香,又含有糯米酒的香气成分。     

基于VB的自动绘制立面图程序的设计

在建筑物采光分析测绘中,需提供大量建筑物的立面图,用于建模和后续采光分析。在以往的生产中,主要依靠在CAD平台下手工成图,生产效率低下,且容易出错。因此,开发一种自动绘制立面图的程序显得尤为迫切。本文以VB 6.0为开发环境,运用ActiveX Automation提供的接口进行AutoCAD的二次开发,重点阐述了利用VB控制Au-toCAD实现自动绘制立面图的思路和算法。投入运行两年来,极大地减轻了内业工作量,并有利于成果的标准化。     

多层分区空间负荷预测结果综合调整的区间方法

提出了一种新的能计及不确定性因素的空间负荷预测结果综合调整的区间方法.首先建立了多层分区的空间负荷预测区间模型,将预测单元分为总量层、数据收集层和仿真层,既能结合趋势法和仿真法的优点,又能在保证足够土地划分解析度和预测精度的前提下有效控制数据收集的工作量.然后提出了基于该模型的空间负荷预测综合调整区间方法,这是一种在信息不完备条件下的负荷分布估计方法,解决了实际中空间负荷预测结果综合调整的难题.最后通过实例说明了该方法的实用性和有效性.     

Theoretic model of myocardial revascularization by far infrared laser and experimental validation

A theoretic model of myocardial revascularization by a far infrared laser has been established and a quantificational rela- tionship between the aperture of laser channel and parameters of laser has been concluded according to thermodynamics and the law of interaction of far infrared laser and myocardium. The experiment of a carbon dioxide laser revascularization in porcine myocardium has been done for different laser powers and irradiation time. The relative errors between experi- mental result and theoret...     

Mass spectrometric detection of marker peptides in tryptic digests of gelatin: A new method to differentiate between bovine and porcine gelatin

Gelatin is a mixture of polypeptides obtained by hydrolysis of collagen primarily from bovine and porcine skin and bones. The similarity between different gelatins makes it difficult to trace their species origin. In this work, a new method for differentiation between bovine and porcine gelatin was developed based on detection and identification of marker peptides in digested gelatins. Sequence alignment analysis indicates that bovine and porcine Type I collagen contain differential sequences. The gelatins were digested by trypsin, and the resulting peptides were analyzed by high performance liquid chromatography/tandem mass spectrometry (HPLC–MS/MS). The marker peptides specific for bovine and porcine were successfully detected in the digested bovine and porcine gelatin, respectively. Comparative analysis indicated that more marker peptides could be detected in gelatin with a higher mean molecular weight. It was found that proline hydroxylation was a key factor affecting the peptide identification. For peptides such as GPPGSAGSPGK and GPPGSAGAPGK detected in digested bovine and porcine gelatin, respectively, the sequence should be verified manually since the mass shift caused by proline hydroxylation can be confused with the mass difference between Ser and Ala residues. The results indicate that detection of marker peptides in the digested gelatin sample using HPLC–MS/MS is an effective method to differentiate between bovine and porcine gelatin.     

健康人体及保健品中乳酸菌和双歧杆菌的抗药性分析

目的:研究常用抗生素对来自健康人体和保健品的乳酸菌的影响.为构建乳酸菌和双歧杆菌食品级基因克隆和表达系统,为研制益生菌食品级微生态制剂而筛选无耐药性受体菌株和安全菌株.为乳酸菌的抗性筛选提供科学依据.方法:采用标准药敏纸片琼脂扩散法(K-B法),以金黄色葡萄球菌ATCC 25923为标准对照菌株,研究15株乳酸球菌,14株乳酸杆菌,5株双歧杆菌对8大类19种常用抗生素的敏感性.结果:15株乳酸球菌对氨苄西林、青霉素G、头孢霉素、头孢曲松、卡那霉素、四环素、红霉素、氯霉素均敏感或中度敏感:对磺胺、多黏菌素B均耐药;对其它抗生素表现出不同药敏性.14株乳杆菌都对氨苄西林、青霉素G、头孢霉素、卡那霉素、红霉素、四环素、氯霉素敏感或中度敏感;对磺胺、甲氧嘧啶、多黏菌素B均耐药:对其它表现出不同药敏性.5株双歧杆菌均对氨苄西林、青霉素G、头孢霉素、卡那霉素、甲氧苄氨嘧啶、红霉素、四环素、氯霉素敏感或中度敏感;对庆大霉素、阿米卡星、链霉素、磺胺、杆菌肽、环丙沙星均耐药;对其它抗生素表现出不同药敏性.     

外在因素对芦荟多糖提取率的影响

研究结果表明,芦荟品种、干燥方法及冷藏过程对芦荟多糖提取率有一定的影响,适合芦荟多糖提取的芦荟品种是库拉索芦荟;采用冻干技术,能提高芦荟多糖的提取率;随着芦荟鲜叶冷藏时间的延长,芦荟多糖的提取率明显降低。     

超声对酵母细胞膜通透性的影响

用不同参数的变幅杆浸入式超声波作用酵母细胞,测定细胞内核酸、蛋白质及FDP(1,6-二磷酸果糖)3种物质渗出率,再用透射电镜观察细胞形态。探讨超声场对酵母细胞膜通透性影响的最佳参数及机理。结果表示,超声场的最佳参数是:电功率500W、脉冲时间3s、间隙时间4s、脉冲总时间共225s,影响酵母细胞膜通透性变化的主要机制是空化作用使细胞膜在一定程度上损伤产生穿孔效应,细胞膜的通透性提高。     

萝卜汁与苹果汁复合饮料的研制

研究采用萝卜与苹果为原料，利用酶水解提高出汁率，结合热澄清与明胶沉淀优化澄清的效果，并通过止交试验确定参数，制成果蔬复合汁。结果表明：以萝卜汁：苹果汁=1：2，加糖量为10％，柠檬酸为0．01％，稳定剂为0．1％海藻酸钠 0．1％黄原胶，制成的萝卜苹果复合汁营养丰富，口感独特。     

烤烟新品种中烟90选育及其特征特性

烤烟新品种中烟90是根据基因重组和累加效应原理,通过 G-28、单育2号和净叶黄三亲本复合杂交,经加代繁殖和混合选择,于1990年育成。1991年通过省级品种审定。1992年通过全国烟草品种审定。其原烟多桔黄或金黄色,光泽较强,结构疏松,厚度适中。还原糖19.30%,尼古丁2.24%,总氮1.85%,钾(K_2O)2.48%,糖碱比8.62,氮碱比0.82,主要化学成分适宜,比例较协调。卷烟评吸香气质较好,香气量较多,劲头适中,燃烧性好。烟叶外观质量与 G-28和NC89相近,内在品质优于 NC89。与 G-28相当。经六年多点试验、全国区试和生产示范,中烟90平均亩产176.3kg,均价2.89元,上等烟25.22%,亩产值513.72元。分别比对照品种增产17.64—27.81%,均价提高0.28—1.14元,上等烟增加2.67—8.28个百分点,亩增收102.84—165.92元,提高30.86—38.28%。高抗黑胫病,抗气候斑点病,耐赤星病和花叶病。主要农艺性状遗传相对稳定。具有优质、稳产、多抗(耐)、适应性广、抗逆力较强、耐肥和易烘烤等特点。