米曲霉固态发酵麻疯树籽饼产中性蛋白酶条件的研究

以实验室筛选出的米曲霉(Aspergillus oryzae)Asp-1为菌种,麻疯树籽饼为发酵主料,采用固态发酵法生产中性蛋白酶.考察了固态发酵培养基液固比、初始pH、接种量等发酵条件,以及麻疯树籽饼中添加麸皮、碳源、氮源对该菌株产酶能力的影响.经单因素和正交实验获得最佳发酵条件为:培养基液固比1:1,初始pH 6,接种量每10 g培养基含有1×108个孢子,培养基中麸皮添加量10%、葡萄糖添加量0.5%、蛋白胨添加量1%.最佳条件下发酵2 d酶活可达3 759.8 U/g.     

合成单宁在棉锦交织物染色中的应用

采用合成单宁防止活性染料沾染棉锦交织物的锦纶组分,研究了合成单宁在锦纶和棉上的吸附差异,探讨了合成单宁预处理条件对防染效果的影响,并分析了染料结构对合成单宁防染作用的影响效果.结果表明,合成单宁在锦纶上的吸附量远高于棉,能有效阻止活性染料沾染锦纶组分;防染效率随着预处理时的pH值降低、温度升高和合成单宁用量增加而增加;染料I/O值越大,亲水性越强,分子结构越大,所含磺酸基数越多,合成单宁的防染效果越好.     

Effect of fermented milk prepared with two probiotic strains on Japanese cedar pollinosis in a double-blind placebo-controlled clinical study

There has been much interest in the potential of using probiotic bacteria for treating allergic diseases. A double-blind, placebo-controlled study was conducted to examine the effectiveness of Lactobacillus GG (LGG) and L. gasseri TMC0356 (TMC0356) in alleviating Japanese cedar pollinosis (JCP), a seasonal allergic rhinitis caused by Japanese cedar pollen. Fermented milk prepared with the tested bacteria or placebo yoghurt was administered to 40 subjects with a clinical history of JCP for 10 weeks. Subjective symptoms, self-care measures and blood samples were compared between the two groups. Peripheral blood mononuclear cells (PBMCs) were collected from seven patients with JCP and in vitro cytokine production by the isolated PBMCs was analysed in the presence of heat-killed lactic acid bacteria. Consumption of the fermented milk significantly decreased the mean symptom score for nasal blockage after 9 weeks (P<0.05) and mean symptom-medication scores after 9 and 10 weeks when compared with the placebo group (P<0.01 and P<0.05 respectively). The tested strains of lactic acid bacteria affected cytokine production by isolated PBMCs in vitro in a strain-dependent manner. LGG significantly inhibited IL-4 and IL-5 production by PBMCs stimulated by both Cry j 1 and PHA. TMC0356 only suppressed IL-5 production stimulated by PHA. The fermented milk prepared with LGG and TMC0356 might be beneficial in JCP because of its effect on nasal blockage. The effects of LGG and TMC0356 might arise at least partly from their specific down-regulation of the human Th2 immune response.     

新原料市场应用的若干思考

找出目前纺织新材料在国内市场中存在的问题,以纽代尔、麻塞尔纤维开发与应用的成功案例说明新型纺织纤维和纱线应有差异化的市场定位,在开发应用过程中,应找到新型纺织纤维与纱线技术之间的内在联系.重点分析了思维创新对产品创新与开发的影响.     

螺旋槽干气密封气膜刚度测试与稳定性分析

干气密封的关键技术是动压效应和稳定性问题,而气膜刚度是影响振动稳定性的主要参数之一。采用高精度改进型的电涡流微型传感器和Labview软件,并采用一系列的抗干扰措施,建立了螺旋槽干气密封的测试系统。通过测量干气密封的轴向静环振幅和轴向气膜振幅,而获得气膜刚度,并分析了其气膜振动的稳定性。试验研究表明：随着转速的增加和压力的升高,气膜刚度随之增大,气膜稳定性越好。本例中：压力变化范围为0.2-0.5MPa,转速变化范围为500 - 3000 r/min,得气膜刚度值的变化范围为0.01 - 0.03 KN/μm,且气膜刚度分别与压力和转速基本成线性关系;试验中测试了三种不同螺旋角的样机,得螺旋角为74°时,气膜刚度最大,气膜稳定性最好,选取合适的螺旋角可增加气膜的稳定性,从而对结构参数的优化提供了指导。     

螺杆膨胀机电液调速系统的建模与仿真分析

以螺杆膨胀机电液调速系统为研究对象,用机理分析法建立了系统的数学模型,并在MATLAB/SIMULINK环境中分别对转速控制的鲁棒性和甩负荷试验以及功率控制的调节阀扰动进行了仿真分析.结果显示控制效果理想,基本与实际相符,证实了数学模型建立的正确性,得到的仿真模型对将来系统的优化设计与调试维护具有一定的实用价值.     

Binding properties of bovine colostral anti-dinitrophenyl (dnp) immunoglobulins g2 (igg2)

The number of combining sites per mole of bovine colostral anti-DNP IgG2 was found to be 2 and the total affinity constant was 0.81 X 10(4) M-1. Unlike bovine colostral IgG1, the nonspecific binding of H3-epsilon-DNP-1-lysine by IgG2 as a function of increased concentrations, did not show a negative, cooperative slope. Spectral measurements made with anti-DNP IgG2 in the reference cell vs. anti-DNP IgG2 plus hapten in the experimental cell revealed a hypochromic, red shift from 363 to 365 nm. If the reference cell contained hapten, a red shift from 280 to 283 nm and an enhancement in the extinction coefficient of IgG2 was observed. These spectral changes were not observed if nonspecific IgG2 was substituted for anti-DNP IgG2 in the experimental cell. The enhancement in the extinction coefficient was interpreted to be due to a possible exposure of previously buried tryosine and tryptophan residues.     

Studies on the antitesticular action of DL-6-(N-2-pipecolinomethyl)-5-hydroxy-indane (PMHI) in the rat

Both prepubertal and adult rats were treated with a single oral dose of either 60 mg or 120 mg of dl-6-(N-pipecolinomethyl)-5-hydroxy indane maleate (PMHI) per kg of body weight. Their testicular weights were drastically reduced compared with those of the controls. A follow-up, beginning on the third day post-treatment and continuing for a period of 50 days, showed that the body weight growth of PMHI-treated rats was not retarded. The hormonal profile indicated that, except for FSH which showed a transitory elevation in PMHI-treated immature rats, the serum levels of LH, estrogen, and testosterone were indistinguishable from those of the controls. Testicular histology revealed that the spermatogenic process in PMHI-treated rats recovered at a dose-related rate. EM sections of testes of adult rats indicated that cytoplasmic vacuolation appeared in the Sertoli cells 5 h post-treatment. The consequent cascade of arrested spermiogenesis included abnormal acrosomal condensation of spermatids and sloughing of polynucleated spermatids. Some spermatocytes also seemed to be affected, but spermatogonia and Leydig cells remained intact. These results indicate the PMHI acts primarily on Sertoli cells and causes arrest in the spermiogenetic stage of the spermatids. At a higher and toxic dose of PMHI, however, the earlier germinal elements might also be affected, due to the extensive damage to the supporting Sertoli cells.     

有界随机噪声参数激励下碰撞系统的矩稳定性

研究了单自由度线性单边碰撞系统在有界随机噪声参数激励下系统的矩稳定性问题.用Zhuravlev变换将碰撞系统转化为连续的非碰撞系统,然后用随机平均法得到了关于慢变量的随机微分方程.利用伊藤法则给出了系统一、二阶矩满足的常微分方程,根据微分方程的稳定性理论得到了系统一阶矩稳定充分必要条件的解析表达式和二阶矩稳定充分必要条件的数值算法,并对理论结果用数值方法进行了仿真计算.理论分析和数值仿真表明,无论是相对于一阶矩还是二阶矩的稳定性,随着随机激励振幅变大,系统的稳定性区域变小从而使得系统变得不稳定.而当调谐参数趋于零系统达到参数主共振情形时,系统的稳定性区域变得最小.当随机噪声强度逐渐变小趋于零时,由二种矩稳定性给出的稳定性区域变得一致.在一定的参数区域内,随机噪声使得系统稳定化.     

火试金重量法测定导电银浆中银量

报导运用火试金重量法测定导电银浆中银含量的方法。考察了称样载体、焙烧温度、铅箔用量、灰吹温度对银含量分析结果的影响,结果表明样品用铅箔作称重载体,250 ℃焙烧,15 g铅箔包裹,900 ℃灰吹是比较理想的方案。实验称取与样品中银量相当的金属银按试样的测定步骤和环境条件分析,银量的回收率作为补正因子k对试样分析结果进行补正。方法回收率为99.6 %～100.2%。对实际样品进行分析,测定值与推荐值相一致,相对标准偏差为0.51%和0.71 %（n=5）。