Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq

Hfq is a pleiotropic regulator that mediates several aspects of bacterial RNA metabolism. The protein notably regulates translation efficiency and RNA decay in Gram-negative bacteria, usually via its interaction with small regulatory RNAs. Previously, we showed that the Hfq C-terminal region forms an amyloid-like structure and that these fibrils interact with membranes. The immediate consequence of this interaction is a disruption of the membrane, but the effect on Hfq structure was unknown. To investigate details of the mechanism of interaction, the present work uses different in vitro biophysical approaches. We show that the Hfq C-terminal region influences membrane integrity and, conversely, that the membrane specifically affects the amyloid assembly. The reported effect of this bacterial master regulator on membrane integrity is discussed in light of the possible consequence on small regulatory RNA-based regulation.     

Postischemic Neuroprotection of Aminoethoxydiphenyl Borate Associates Shortening of Peri-Infarct Depolarizations

Brain stroke is a highly prevalent pathology and a main cause of disability among older adults. If not promptly treated with recanalization therapies, primary and secondary mechanisms of injury contribute to an increase in the lesion, enhancing neurological deficits. Targeting excitotoxicity and oxidative stress are very promising approaches, but only a few compounds have reached the clinic with relatively good positive outcomes. The exploration of novel targets might overcome the lack of clinical translation of previous efficient preclinical neuroprotective treatments. In this study, we examined the neuroprotective properties of 2-aminoethoxydiphenyl borate (2-APB), a molecule that interferes with intracellular calcium dynamics by the antagonization of several channels and receptors. In a permanent model of cerebral ischemia, we showed that 2-APB reduces the extent of the damage and preserves the functionality of the cortical territory, as evaluated by somatosensory evoked potentials (SSEPs). While in this permanent ischemia model, the neuroprotective effect exerted by the antioxidant scavenger cholesteronitrone F2 was associated with a reduction in reactive oxygen species (ROS) and better neuronal survival in the penumbra, 2-APB did not modify the inflammatory response or decrease the content of ROS and was mostly associated with a shortening of peri-infarct depolarizations, which translated into better cerebral blood perfusion in the penumbra. Our study highlights the potential of 2-APB to target spreading depolarization events and their associated inverse hemodynamic changes, which mainly contribute to extension of the area of lesion in cerebrovascular pathologies.     

Double‐frequency buck converter as a candidate topology for integrated envelope elimination and restoration applications in power supply of RFPAs

This paper proposes the use of double‐frequency (DF) buck converter architecture consisting of a merged structure of high and low frequency buck cells as a candidate topology for envelope elimination and restoration (EER) applications and integrated power supply of RF power amplifiers (RFPA) to obtain favorable tradeoffs in terms of efficiency, switching ripple, bandwidth, and tracking capability. It is shown that having two degrees of freedom in designing the DF buck helps to achieve high efficiency, low output ripples, and tracking capability with low ripples, simultaneously. A comparison analysis is done with regards to the mentioned performance indexes with the standard and three‐level buck converters; in addition, the results are validated in HSPICE in BSIM3V3 0.35‐µm CMOS process. Copyright © 2015 John Wiley & Sons, Ltd.     

Dimensionality reduction and classification of hyperspectral image data using sequences of extended morphological transformations

This work describes sequences of extended morphological transformations for filtering and classification of high-dimensional remotely sensed hyperspectral datasets. The proposed approaches are based on the generalization of concepts from mathematical morphology theory to multichannel imagery. A new vector organization scheme is described, and fundamental morphological vector operations are defined by extension. Extended morphological transformations, characterized by simultaneously considering the spatial and spectral information contained in hyperspectral datasets, are applied to agricultural and urban classification problems where efficacy in discriminating between subtly different ground covers is required. The methods are tested using real hyperspectral imagery collected by the National Aeronautics and Space Administration Jet Propulsion Laboratory Airborne Visible-Infrared Imaging Spectrometer and the German Aerospace Agency Digital Airborne Imaging Spectrometer (DAIS 7915). Experimental results reveal that, by designing morphological filtering methods that take into account the complementary nature of spatial and spectral information in a simultaneous manner, it is possible to alleviate the problems related to each of them when taken separately.     

Chronic-Antibiotics Induced Gut Microbiota Dysbiosis Rescues Memory Impairment and Reduces β-Amyloid Aggregation in a Preclinical Alzheimer’s Disease Model

Alzheimer’s disease (AD) is a multifactorial pathology characterized by β-amyloid (Aβ) deposits, Tau hyperphosphorylation, neuroinflammatory response, and cognitive deficit. Changes in the bacterial gut microbiota (BGM) have been reported as a possible etiological factor of AD. We assessed in offspring (F1) 3xTg, the effect of BGM dysbiosisdysbiosis in mothers (F0) at gestation and F1 from lactation up to the age of 5 months on Aβ and Tau levels in the hippocampus, as well as on spatial memory at the early symptomatic stage of AD. We found that BGM dysbiosisdysbiosis with antibiotics (Abx) treatment in F0 was vertically transferred to their F1 3xTg mice, as observed on postnatal day (PD) 30 and 150. On PD150, we observed a delay in spatial memory impairment and Aβ deposits, but not in Tau and pTau protein in the hippocampus at the early symptomatic stage of AD. These effects are correlated with relative abundance of bacteria and alpha diversity, and are specific to bacterial consortia. Our results suggest that this specific BGM could reduce neuroinflammatory responses related to cerebral amyloidosis and cognitive deficit and activate metabolic pathways associated with the biosynthesis of triggering or protective molecules for AD.     

A quantitative and comparative analysis of endmember extraction algorithms from hyperspectral data

Linear spectral unmixing is a commonly accepted approach to mixed-pixel classification in hyperspectral imagery. This approach involves two steps. First, to find spectrally unique signatures of pure ground components, usually known as endmembers, and, second, to express mixed pixels as linear combinations of endmember materials. Over the past years, several algorithms have been developed for autonomous and supervised endmember extraction from hyperspectral data. Due to a lack of commonly accepted data and quantitative approaches to substantiate new algorithms, available methods have not been rigorously compared by using a unified scheme. In this paper, we present a comparative study of standard endmember extraction algorithms using a custom-designed quantitative and comparative framework that involves both the spectral and spatial information. The algorithms considered in this study represent substantially different design choices. A database formed by simulated and real hyperspectral data collected by the Airborne Visible and Infrared Imaging Spectrometer (AVIRIS) is used to investigate the impact of noise, mixture complexity, and use of radiance/reflectance data on algorithm performance. The results obtained indicate that endmember selection and subsequent mixed-pixel interpretation by a linear mixture model are more successful when methods combining spatial and spectral information are applied.     

An application framework for developing collaborative handheld decision-making tools

This paper describes an application framework supporting collaborative handheld decision-making (CHDM). The main characteristics of the framework are: (1) extensive usage of visual elements and gestures; and (2) independence from specific decision-making methods, processes and tasks. The research departed from the analysis and systematisation of several CHDM scenarios, highlighting repeatable behaviour across multiple decision-making contexts. From these scenarios, we distilled a coherent set of common functional requirements organised in three main categories: process, macro- and micro-functionality. The proposed framework has been validated at length through the development of several CHDM tools. Six different tools are described in the paper. The main contribution of this work is a common foundation for developing CHDM tools.     

Web-GIS models: accomplishing modularity with aspects

Spatial concerns of Web geographical information systems (Web-GIS) are inherently crosscutting and volatile: crosscutting because they affect multiple functionalities of Web-GIS systems, and volatile because their status may change often. If these concerns are not modularized properly, the quality of Web-GIS services, particularly with regard to adaptation and evolution, can be severely compromised. This paper uses aspect-orientation to model crosscutting and volatile spatial concerns. By modeling both types of concerns, crosscutting and volatile, as candidate aspects, one can use dynamic weaving to add or remove them from a system at runtime. The aspect-oriented approach proposed starts with the identification and specification of crosscutting concerns and follows by composing these using modeling aspects using a transformation approach, an aspect-oriented modeling technique. The conflicts that can emerge due to the composition order are also taken into consideration. Finally, this paper proposes a set of reusable GIS crosscutting concerns, documenting them in a concern catalogue.     

Stability and mechanical evaluation of bovine pericardium cross-linked with polyurethane prepolymer in aqueous medium

The present study investigates the potential use of non-catalyzed water-soluble blocked polyurethane prepolymer (PUP) as a bifunctional cross-linker for collagenous scaffolds. The effect of concentration (5, 10, 15 and 20%), time (4, 6, 12 and 24 h), medium volume (50, 100, 200 and 300%) and pH (7.4, 8.2, 9 and 10) over stability, microstructure and tensile mechanical behavior of acellular pericardial matrix was studied. The cross-linking index increased up to 81% while the denaturation temperature increased up to 12 °C after PUP crosslinking. PUP-treated scaffold resisted the collagenase degradation (0.167 ± 0.14 mmol/g of liberated amine groups vs. 598 ± 60 mmol/g for non-cross-linked matrix). The collagen fiber network was coated with PUP while viscoelastic properties were altered after cross-linking. The treatment of the pericardial scaffold with PUP allows (i) different densities of cross-linking depending of the process parameters and (ii) tensile properties similar to glutaraldehyde method.     

Patenting and licensing by Spanish firms: Available survey evidence

This paper presents the results of the OEPM (Spanish Patent and Trademark Office)/OECD (Organisation for Economic Co-operation and Development) survey on the economic and financial uses of patents carried out in 2008 among Spanish business applicants of OEPM patents. It also summarizes evidence from previous surveys. All available information indicates that only a few Spanish firms holding patents license them out. Results from the OEPM/OECD survey also reveal that obtaining public support is the first financial use of patents for Spanish firms. Responses to the same OECD questionnaire by EPO patent applicants from several European countries are quite different: licensing out is an extended practice among EPO patent holders from different countries, including Spain, and convincing venture capitalists and private investors are their two most important financial uses of patents. In our view this suggests that there are significant differences across European countries as regards the development of national technology markets and results from international surveys are driven by responses from the largest and more developed countries. The drivers and actors in markets for patents that only protect inventions nationally may be quite distinct from those in markets for patents that protect inventions regionally, such as EPO patents within Europe.