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31.
SZ Zhou S Cooper LY Kang L Ruggieri S Heimfeld A Srivastava HE Broxmeyer 《Canadian Metallurgical Quarterly》1994,179(6):1867-1875
Recombinant adeno-associated virus 2 (AAV) virions were constructed containing a gene for resistance to neomycin (neoR), under the control of either the herpesvirus thymidine kinase (TK) gene promoter (vTK-Neo), or the human parvovirus B19 p6 promoter (vB19-Neo), as well as those containing an upstream erythroid cell-specific enhancer (HS-2) from the locus control region of the human beta-globin gene cluster (vHS2-TK-Neo; vHS2-B19-Neo). These recombinant virions were used to infect either low density or highly enriched populations of CD34+ cells isolated from human umbilical cord blood. In clonogenic assays initiated with cells infected with the different recombinant AAV-Neo virions, equivalent high frequency transduction of the neoR gene into slow-cycling multipotential, erythroid, and granulocyte/macrophage (GM) progenitor cells, including those with high proliferative potential, was obtained without prestimulation with growth factors, indicating that these immature and mature hematopoietic progenitor cells were susceptible to infection by the recombinant AAV virions. Successful transduction did not require and was not enhanced by prestimulation of these cell populations with cytokines. The functional activity of the transduced neo gene was evident by the development of resistance to the drug G418, a neomycin analogue. Individual high and low proliferative colony-forming unit (CFU)-GM, burst-forming unit-erythroid, and CFU-granulocyte erythroid macrophage megakaryocyte colonies from mock-infected, or the recombinant virus-infected cultures were subjected to polymerase chain reaction analysis using a neo-specific synthetic oligonucleotide primer pair. A 276-bp DNA fragment that hybridized with a neo-specific DNA probe on Southern blots was only detected in those colonies cloned from the recombinant virus-infected cells, indicating stable integration of the transduced neo gene. These studies suggest that parvovirus-based vectors may prove to be a useful alternative to the more commonly used retroviral vectors for high efficiency gene transfer into slow or noncycling primitive hematopoietic progenitor cells, without the need for growth factor stimulation, which could potentially lead to differentiation of these cells before transplantation. 相似文献
32.
Research in the 1980s uncovered ubiquitous neuropeptide-receptor distribution in brain structures associated with emotional processing, and throughout many organ systems. This finding supported neuropeptides as biochemical substrates of emotion, and the neuropeptide-receptor network as a parasynaptic system crossing traditional brain-body boundaries. The medical relevance of these findings was affirmed by psychoneuroimmunology research: neuropeptides help to regulate immunocyte trafficking, there is bidirectional communication between nervous and immune system components, immunocytes produce neuropeptides, and nerve cells produce immune-associated cytokines. In the past decade, the concept of a unified psychosomatic network has been strengthened by animal and human research demonstrating relationships between behavior and neuropeptide-mediated regulation of immune functions. Research on emotional expression or disclosure in healthy human subjects as well as in cancer and HIV-positive patients has shown significant positive correlations with clinically relevant immune functions and/or positive health outcomes. Psychosocial interventions emphasizing emotional expression or active coping have evidenced survival benefits in breast cancer and melanoma. These findings suggest that emotional expression generates balance in the neuropeptide-receptor network and a functional healing system. Emotional expression is also a marker for psychospiritual vitalization, and further research should evaluate links between energy-based models of health and neuropeptide-receptor-based models under the rubric of an informational paradigm. 相似文献
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何纪红 《数码设计:surface》2012,(7):133-134
研究丹东地区满族舞蹈音乐的历史特点与发展,通过研究丹东地区满族风俗对满族民间舞蹈音乐的影响,分析丹东地区特有的四种满族节庆舞蹈,发现满族民间舞蹈音乐在调式、旋律、节奏及形式方面的特点,并根据民间舞蹈音乐的特点提出丹东地区民间舞蹈音乐发展的方向。本文主要研究丹东地区满族风俗对满族民间舞蹈音乐的影响与发展。 相似文献
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何绍林 《电脑与微电子技术》2012,(17):29-31
针对均值滤波和中值滤波的不足,提出一种改进的基于双阈值滤波算法。该算法通过两个阈值和均值的关系来判断当前点应取的值。和传统的滤波算法相比.该算法在滤出噪声的同时,能最大限度保留原始图像信息,滤波效果较好。 相似文献
37.
Zhang Zhe Wang Chunyu Qiu Weichao Qin Wenhu Zeng Wenjun 《International Journal of Computer Vision》2021,129(3):703-718
International Journal of Computer Vision - Occlusion is probably the biggest challenge for human pose estimation in the wild. Typical solutions often rely on intrusive sensors such as IMUs to... 相似文献
38.
研究了煤矿安全监测及灾害预警的检测问题,提高检测的准确性。已有的煤矿安全监测技术多是基于单层传感网络设计,当瓦斯等危险气氛浓度泄露受到外界干扰,容易发生检测节点特征退化,传统方法抗噪能力差,效率低,监测结果不稳定。为了解决这一问题,提出一种基于改进最大熵算法的井下实时监控与灾害监测预警系统设计方法。设计了基于抗干扰的最大熵检索建模功能,快速计算跳数和能耗信息,实现安全隐患的准确检测。仿真结果表明,系统能够大幅降低干扰风险,取得良好的效果。 相似文献
39.
本文以福建高唐水电站灯泡贯流式水轮机真机为研究对象,对贯流式水轮机满负荷工况、额定工况和低负荷工况进行了三维非定常数值模拟,各工况导、桨叶均为各自对应的最优协联角度,来分别研究三种工况压力脉动特性和尾水管的流动情况.结果表明,灯泡贯流式水轮机在导叶进口、转轮进口和尾水管进口压力脉动主要是受叶片通过频率影响,即由转轮旋转所引起的,而在尾水管出口主要受低频压力脉动的影响.同时,满负荷工况时,脉动幅值最小,尾水管流线相对分布均匀,形成的涡量最少,额定工况至低负荷工况,幅值越来越大,尾水管流线也越来越紊乱,漩涡数量明显增加. 相似文献
40.