Comparison of the Cisterna Maturation-Progression Model with the Kiss-and-Run Model of Intra-Golgi Transport: Role of Cisternal Pores and Cargo Domains

The Golgi complex is the central station of the secretory pathway. Knowledge about the mechanisms of intra-Golgi transport is inconsistent. Here, we compared the explanatory power of the cisterna maturation-progression model and the kiss-and-run model. During intra-Golgi transport, conventional cargoes undergo concentration and form cisternal distensions or distinct membrane domains that contain only one membrane cargo. These domains and distension are separated from the rest of the Golgi cisternae by rows of pores. After the arrival of any membrane cargo or a large cargo aggregate at the Golgi complex, the cis-Golgi SNAREs become enriched within the membrane of cargo-containing domains and then replaced by the trans-Golgi SNAREs. During the passage of these domains, the number of cisternal pores decreases. Restoration of the cisternal pores is COPI-dependent. Our observations are more in line with the kiss-and-run model.     

Physical filtration efficiency analysis of a polyaniline hybrid composite filter with graphite oxide for particulate matter 2.5

Particulate matter (PM) 2.5 pollution is a prevalent environmental and public health issue that has raised serious global concerns. Because standard heating, ventilation, and air conditioning filters are incapable of filtering out PM 2.5 particles efficiently, different methods of PM 2.5 filtration, such as physical filtration and electrostatic filtration, are under investigation to develop a filter with a high filtration efficiency and a low pressure drop. According to various studies, pressure drop has a significant influence on the filtration efficiency. An equation for the theoretical trend was generated based on the composite data gathered from similar filtration studies and was used to evaluate the relationship between pressure drop and filtration efficiency. Here, the theoretical equation indicated that the filtration efficiency increased as the pressure drop on a filter increased until 0.01 psi where the efficiency remained near constant at approximately 99.9%. In this study, we introduce a graphite oxide (GO) and polyaniline (PANi) composite hybrid filter in order to create a low-pressure (1.2 psi) drop filter. By adding GO flakes to the PANi matrix, we not only produced a highly permeable filter while allowing continuous gas flow, but also achieved a remarkable and highly effective PM 2.5 filter with a filtration efficiency of 99.7 ± 0.08%.     

A methodology for determining the true stress-strain curve of SA-508 low alloy steel from a tensile test with finite element analysis

The true stress-strain curve of a material should be determined for plastic property input to numerical analysis. This study proposes a simple methodology for determining the true stress-strain curve of SA-508 Grade 3 Class 1 low alloy steel using limited information from a general tensile test with finite element analysis. Measured engineering stresses and strains can be reasonably converted to true stresses and strains under uniform deformation before necking. True stress-strains are difficult to determine after necking because of nonuniform deformation without specialized measurement techniques. Five post-necking strain hardening models are considered, namely, linear, swift, Ludwick, Hollomon-linear (HL) and Hollomon-linear-constant (HLC) models. The equations for each model can be determined using the results of the tensile test, which include the true stress-strain value at the maximum load point and the corrected true stress-strain value at the fracture point plus the Considere instability criterion. The HL and HLC models suggested that the engineering stress-strains from the finite element analysis are consistent with the experimental results.     

Molecular dynamics study on the effect of solution-wall interaction potential on the properties of solution in uniformly charged hydrophobic channel

We numerically investigate the effect of the solution-wall (i.e., water-wall and ion-wall) interaction potential on the properties of confined aqueous solution by using molecular dynamics (MD) simulations. The effect is determined by comparing results obtained from the MD simulation using the Lennard-Jones (L-J) potential for the water-wall and ion-wall interactions and those using a purely repulsive potential, i.e., the Weeks-Chandler-Andersen-like potential. In the MD simulations, 680 water molecules and 20 chloride ions are included between uniformly charged plates that are separated by 2.6 nm. The results show that the properties of solution are influenced only in the region close to the wall: The water molecules are more densely packed for the case of the L-J potential. Comparison of the results of the MD simulations in the case of the L-J potential with those provided by solving the Poisson-Boltzmann equation, we found that classical continuum theory fails to predict the ion density and electrostatic potential distributions in the region near to the wall, but far way from the wall, the prediction from the continuum theory is in line with the MD simulation.     

Preparation and electrostatic properties of antistatic acrylics

Very good antistatic acrylic films or fabrics can be prepared by exposing the acrylic samples containing oxidizing agent to aniline vapor. The conductivity, the maximum static charge, and the half-life time are used for estimation of electrostatic properties. The effects of the content of polyaniline and the temperature and time of exposure to aniline vapor on the electrostatic properties have been investigated. The stability of conductivity to ambient air exposure and the temperature dependence of conductivity for antistatic acrylic films have also been investigated.     

Refinement method for structure from stereo motion

A new method for overcoming bas-relief ambiguity, using a calibrated stereo image sequence, is presented. The proposed method uses a direct method for motion estimation as the initial guess, and refines both stereo and motion displacement with sub-pixel accuracy. Experimental results show that the proposed algorithm improves the estimation accuracy under such ambiguity     

Oxygen barrier properties of biaxially oriented polypropylene/polyvinyl alcohol blend films

The biaxially oriented PP/PVA blend film was prepared and had the higher oxygen barrier property by about 130 times than that of a biaxially oriented PP film. When the viscosity ratio (η_d(PVA)/η_m(PP)) decreased, the dispersed PVA phase was developed into platelets during stretching process. Oxygen permeability was dependent on the number and size of PVA platelet. However, the Oxygen permeability was not sensitively changed in above 25 wt% of PVA. To obtain excellent barrier property, the optimum amount of plasticizer and initiator was required. A pasticizer was related to the size and degree of crystallization of PVA platelet. An initiator played the role of a compatibilizer. The oxygen barrier was enhanced with increasing the viscosity of PP and draw ratio. The higher viscosity of PP was advantageous for preventing the delamination of a blend film, and the moisture vapor permeability was not affected with the laminar structure. As a result, the biaxially oriented PP/PVA blend film had the potential of substituting for the PVDC coated BOPP film.     

Incompressible laminar flow near a corner of 90° angle

Numerical integration is conducted for two-dimensional incompressible laminar flow over a 90° corner. Using Newton’s method, the Navier-Stokes equations are generated up toRe=2800, with the result that the corner generates a second bubble nearRe=800. There exist distinct patterns for the evolution of the pressure gradient and the position of a separation point. AsRe is increased the pressure gradient tends to approach zero over the recirculated region and shows sharper variations near the separation and reattachment points. Thus, these results confirm the free streamline model for separation proposed by Sychev.     

Observational method for field performance of prefabricated vertical drains

An exponential formula is used to best-fit theoretical and measured time–settlement (or excess pore pressure) data over the full range of consolidation. The formula fits well theoretical consolidation solutions and measured data regardless of using the incompletely consolidated data, and it is possible to reliably predict the ultimate values. This result has a different trend from those of the hyperbolic and Asaoka (1978) methods. Thus the coefficients of horizontal consolidation and the mobilised discharge capacity q_w(mob) can be expressed in terms of parameters of the exponential formula corresponding to the measured data and the theoretical solutions. The application of the proposed method to six case records on three construction sites (with a maximum drainage path l_m of 7−50 m) indicates that the coefficient of horizontal consolidation for the ideal condition are likely to be used to reconstruct the monitored time–settlement curve and also to adjust the hydraulic and consolidation properties of each monitored point. Based on back-analysis, the mobilised and required discharge capacity for a preliminary design guideline are recommended as: q_w(mob) = (1–5)k_hl_m² and q_w(req) = 19.63k_hl_m², where k_h is the horizontal permeability of soil.