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961.
The present experiments examined the effects of posttraining intrahippocampal injections of the degradative enzyme-resistant methylcarbamyl analog of the bioactive phospholipid platelet-activating factor (mc-PAF) and the platelet-activating factor (PAF) receptor antagonists BN52021 and BN 50730 on memory in male Long-Evans rats trained in a hidden platform version of the Morris water maze. Following an eight-trial training session, rats received a unilateral intrahippocampal injection of mc-PAF (0.5, 1.0, or 2.0 microgram/0.5 microliter), lyso-PAF (1.0 microgram/0.5 microliter), the cell surface PAF receptor antagonist BN 52021 (0.25, 0.5, or 1.0 micrigram/0.5 microliter/, the intracellular PAF receptor antagonist BN 50730 (2.0, 5.0, or 10.0 microgram/0.5 microliter), or vehicle (50% DMSO in 0.9% saline; 0.5 microliter). On a retention test conducted 24 h after training, the escape latencies of rats administered mc-PAF (1.0 or 2.0 microgram) were significantly lower than those of the vehicle-injected controls, demonstrating a memory-enhancing effect of mc-PAF. Injections of lyso-PAF, a structurally similar metabolite of PAF, had no influence on memory, indicating that the memory-enhancing effect of mc-PAF is not caused by membrane perturbation by the phospholipid. The retention test escape latencies of rats administered BN 52021 (0.5 microgram) and BN 50730 (5.0 or 10 microgram) were significantly higher than those of the controls, indicating a memory impairing effect of both PAF antagonists. When mc-PAF, BN 52021, or BN 50730 was administered 2 h posttraining, no effect on retention was observed, indicating a time-dependent effect of the neuroactive substances on memory storage. The findings suggest a role for endogenous PAF in hippocampal-dependent memory processes. 相似文献
962.
JF Richmond S Lu JC Santoro J Weng SL Hu DC Montefiori HL Robinson 《Canadian Metallurgical Quarterly》1998,72(11):9092-9100
DNA vaccination is an effective means of eliciting strong antibody responses to a number of viral antigens. However, DNA immunization alone has not generated persistent, high-titer antibody and neutralizing antibody responses to human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env). We have previously reported that DNA-primed anti-Env antibody responses can be augmented by boosting with Env-expressing recombinant vaccinia viruses. We report here that recombinant Env protein provides a more effective boost of DNA-initiated antibody responses. In rabbits primed with Env-expressing plasmids, protein boosting increased titer, persistence, neutralizing activity, and avidity of anti-Env responses. While titers increased rapidly after boosting, avidity and neutralizing activity matured more slowly over a 6-month period following protein boosting. DNA priming and protein immunization with HIV-1 HXB-2 Env elicited neutralizing antibody for T cell line-adapted, but not primary isolate, viruses. The most effective neutralizing antibody responses were observed after priming with plasmids which expressed noninfectious virus-like particles. In contrast to immunizations with HIV-1 Env, DNA immunizations with the influenza virus hemagglutinin glycoprotein did not require a protein boost to achieve high-titer antibody with good avidity and persistence. 相似文献
963.
并行工程作为一个新的设计哲理在工程实践中的实现手段不尽相同,本文中我们提出了CAD/CAPP并行集成概念,并开发了原型系统CECADP,初步实现了一种并行设计的运行模式。 相似文献
964.
965.
基于ARToolkit Plus的增强现实系统实现及框架研究 总被引:1,自引:0,他引:1
文章首先对基于ARToolkit Plus和WPF的增强现实系统实现的关键技术问题进行了描述,具体讨论了在MVC框架下如何将数据采集层与呈现层进行分划,在保证可用性的同时提供较强的扩展能力,并基于上述框架实现了一个应用于住房装修的实例。 相似文献
966.
967.
针对GPS输出信息的随机延迟特性,基于航位推算(DR)系统惯性器件的实时性与短时高精度,提出了航向差和速度自适应GPS滞后时间检测方法,在此基础上,对GPS和DR数据进行数据对准,提出了自适应GPS滞后时间处理算法。在GPS/DR实际系统中的应用结果表明,本算法能够准确检测出GPS的滞后时间,提高车辆的定位精度。 相似文献
968.
969.
970.
Space-time adaptive processing (STAP) is an effective method for detecting moving target in airborne/spaceborne radar.However,the detection of fast air moving targets from high speed platform is challenged by the range walk of both the clutter and moving targets.It is well known that keystone formatting can be used to compensate for the range walk of multiple moving targets simultaneously without using the knowledge of the motion parameters.However,in the presence of serious Doppler ambiguity of fast air moving targets,distribution of the clutter will be affected by the keystone formatting matched to the ambiguity number of targets,and as a result the STAP performance will degrade.In this paper,a novel STAP method is proposed for the detection of fast air moving targets from high speed platform,which can deal with the range walk of both clutter and targets.Effectiveness of the new method is verified via simulation examples. 相似文献