Proinflammatory macrophage-activating properties of the novel phospholipid diacylglycerol pyrophosphate

We have found that the novel phospholipid diacylglycerol pyrophosphate (DGPP), identified in bacteria, yeast, and plants, but not in mammalian cells, is able to potently activate macrophages for enhanced secretion of arachidonate metabolites, a key event in the immunoinflammatory response of leukocytes. Macrophage responses to DGPP are specific and are not mediated by its conversion into other putative lipid mediators such as phosphatidic acid, lysophosphatidic acid, or diacylglycerol. The responses to DGPP are compatible with a receptor-recognition event because they are blocked by suramin. Intracellular signaling initiated by DGPP includes phosphorylation and activation of the Group IV cytosolic phospholipase A2 and of the extracellular-signal regulated p42 mitogen-activated protein kinase (MAPK) and p44 MAPK, and membrane translocation of the protein kinase C isoenzymes alpha, epsilon, delta. These results establish DGPP as a novel macrophage-activating factor and suggest a potential role for this compound in triggering homeostatic cellular responses.     

Substrate phosphorylation in the protein kinase Cgamma knockout mouse

The phosphorylation state of three identified neural-specific protein kinase C substrates (RC3, GAP-43/B-50, and MARCKS) was monitored in hippocampal slices of mice lacking the gamma-subtype of protein kinase C and wild-type controls by quantitative immunoprecipitation following 32Pi labeling. Depolarization with potassium, activation of glutamate receptors with glutamate, or direct stimulation of protein kinase C with a phorbol ester increased RC3 phosphorylation in wild-type animals but failed to affect RC3 phosphorylation in mice lacking the gamma-subtype of protein kinase C. Our results suggests the following biochemical pathway: activation of a postsynaptic (metabotropic) glutamate receptor stimulates the gamma-subtype of protein kinase C, which in turn phosphorylates RC3. The inability to increase RC3 phosphorylation in mice lacking the gamma-subtype of protein kinase C by membrane depolarization or glutamate receptor activation may contribute to the spatial learning deficits and impaired hippocampal LTP observed in these mice.     

Minimization of the Bode sensitivity integral

In this paper we present two new results on Bode-type integrals. Firstly, we obtain, for a given scalar or multivariable continuous-time plant, the infimum of the Bode sensitivity integral which can be obtained with any stabilizing controller. The result involves the unstable plant poles and, perhaps surprisingly, a subset of the plant nonminimum phase zeros. Secondly, we obtain an apparently new expression for the Bode integral for the complementary sensitivity for a stable discrete-time scalar system.     

A note on knowledge-based programs and specifications

Summary. Knowledge-based program are programs with explicit tests for knowledge. They have been used successfully in a number of applications. Sanders has pointed out what seem to be a counterintuitive property of knowledge-based programs. Roughly speaking, they do not satisfy a certain monotonicity property, while standard programs (ones without tests for knowledge) do. It is shown that there are two ways of defining the monotonicity property, which agree for standard programs. Knowledge-based programs satisfy the first, but do not satisfy the second. It is further argued by example that the fact that they do not satisfy the second is actually a feature, not a problem. Moreover, once we allow the more general class of knowledge-based specifications, standard programs do not satisfy the monotonicity property either. Received: January 1997 / Accepted January 2000     

Turing's Test and the ideology of artificial intelligence

A close examination is offered of the Turing Test, whose lasting influence is ascribed to its power as a myth which is expressive of the ideology that underlies strong AI claims. Arguments are advanced to show that the Test is not a bonafide thought-experiment, nor an acceptable substitute for the question, Can a Machine Think? AI work is held to be highly valuable, but by reason of its achievement of thought-like results through the power of algorithms, not through its supposed emulation of thought. Finally, evidence is offered for thinking that the issue at hand is not only a practical, but an urgent one.     

Cryptic invasions of the crab Carcinus detected by molecular phylogeography

Coastal marine ecosystems world-wide are threatened by invasions of nonindigenous species. The ubiquity of marine sibling species identifiable only by genetic analysis suggests that many invasions are cryptic and therefore undetected, causing an underestimation of the actual number and impacts of invading species. We test this hypothesis with European crabs in the genus Carcinus that have invaded five regions globally. Partial 16S ribosomal RNA gene sequences confirm sibling species status of morphologically similar Atlantic C. maenas and Mediterranean C. aestuarii. Based on 16S rRNA haplotypes, crabs from California, New England and Tasmania were all C. maenas. However, we report the cryptic multiple invasion of both species in Japan and South Africa, where only C. aestuarii and C. maenas, respectively, were previously recognized.