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41.
Jacob T. Heggestad Cassio M. Fontes Daniel Y. Joh Angus M. Hucknall Ashutosh Chilkoti 《Advanced materials (Deerfield Beach, Fla.)》2020,32(2):1903285
“Nonfouling” polymer brush surfaces can greatly improve the performance of in vitro diagnostic (IVD) assays due to the reduction of nonspecific protein adsorption and consequent improvement of signal-to-noise ratios. The development of synthetic polymer brush architectures that suppress adventitious protein adsorption is reviewed, and their integration into surface plasmon resonance and fluorescent sandwich immunoassay formats is discussed. Also, highlighted is a novel, self-contained immunoassay platform (the D4 assay) that transforms time-consuming laboratory-based assays into a user-friendly and point-of-care format with a sensitivity and specificity comparable or better than standard enzyme-linked immunosorbent assay (ELISA) directly from unprocessed samples. These advancements clearly demonstrate the utility of nonfouling polymer brushes as a substrate for ultrasensitive and robust diagnostic assays that may be suitable for clinical testing, in field and laboratory settings. 相似文献
42.
S Cho BT Volpe Y Bae O Hwang HJ Choi J Gal LC Park CK Chu J Du TH Joh 《Canadian Metallurgical Quarterly》1999,19(3):878-889
The generation of nitric oxide (NO) aggravates neuronal injury. (6R)-5,6,7,8-Tetrahydro-L-biopterin (BH4) is an essential cofactor in the synthesis of NO by nitric oxide synthase (NOS). We attempted to attenuate neuron degeneration by blocking the synthesis of the cofactor BH4 using N-acetyl-3-O-methyldopamine (NAMDA). In vitro data demonstrate that NAMDA inhibited GTP cyclohydrolase I, the rate-limiting enzyme for BH4 biosynthesis, and reduced nitrite accumulation, an oxidative metabolite of NO, without directly inhibiting NOS activity. Animals exposed to transient forebrain ischemia and treated with NAMDA demonstrated marked reductions in ischemia-induced BH4 levels, NADPH-diaphorase activity, and caspase-3 gene expression in the CA1 hippocampus. Moreover, delayed neuronal injury in the CA1 hippocampal region was significantly attenuated by NAMDA. For the first time, these data demonstrate that a cofactor, BH4, plays a significant role in the generation of ischemic neuronal death, and that blockade of BH4 biosynthesis may provide novel strategies for neuroprotection. 相似文献
43.
L. Wolfrum und Joh Pinnow 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1914,28(1):30-32
Ohne ZusammenfassungMitteilung aus dem Chemischen Staatslaboratorium zu Bremen. 相似文献
44.
Joh Pinnow 《European Food Research and Technology》1920,40(9-10):243-246
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Effects of the capillary number and size distribution of bubbles on rheological properties of bubble suspensions with different volume fractions were investigated through both experimental and theoretical analyses. Polydispersed bubble suspensions were prepared by using a bubble making device and characterized in steady and dynamic oscillatory shear flows to examine the effect of the bubble size distribution. To predict the rheological properties of the polydispersed bubble suspension, a constitutive equation was modified by applying superposition schemes, i.e., a probability density function was introduced to reflect the size distribution of bubbles. Dilute bubble suspensions showed shear thinning and linear viscoelastic behavior. At small capillary numbers, relative viscosity increased with increasing bubble volume fraction, whereas it decreased as bubble volume fraction increased at relatively large capillary numbers. It was found that rheological properties had been affected significantly by polydispersity of bubble suspensions and theoretical results were in good agreement with the experimental results. POLYM. ENG. SCI., 2010. © 2009 Society of Plastics Engineers 相似文献
48.
Park OK Hahm MG Lee S Joh HI Na SI Vajtai R Lee JH Ku BC Ajayan PM 《Nano letters》2012,12(4):1789-1793
Highly conductive reduced graphene oxide (GO) polymer nanocomposites are synthesized by a well-organized in situ thermochemical synthesis technique. The surface functionalization of GO was carried out with aryl diazonium salt including 4-iodoaniline to form phenyl functionalized GO (I-Ph-GO). The thermochemically developed reduced GO (R-I-Ph-GO) has five times higher electrical conductivity (42,000 S/m) than typical reduced GO (R-GO). We also demonstrate a R-I-Ph-GO/polyimide (PI) composites having more than 10(4) times higher conductivity (~1 S/m) compared to a R-GO/PI composites. The electrical resistances of PI composites with R-I-Ph-GO were dramatically dropped under ~3% tensile strain. The R-I-Ph-GO/PI composites with electrically sensitive response caused by mechanical strain are expected to have broad implications for nanoelectromechanical systems. 相似文献
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50.
Cushing GW Navin JK Valadez L Johánek V Harrison I 《The Review of scientific instruments》2011,82(4):044102
An effusive molecular beam technique is described to measure alkane dissociative sticking coefficients, S(T(g), T(s); ?), on metal surfaces for which the impinging gas temperature, T(g), and surface temperature, T(s), can be independently varied, along with the angle of incidence, ?, of the impinging gas. Effusive beam experiments with T(g) = T(s) = T allow for determination of angle-resolved dissociative sticking coefficients, S(T; ?), which when averaged over the cos?(?)/π angular distribution appropriate to the impinging flux from a thermal ambient gas yield the thermal dissociative sticking coefficient, S(T). Nonequilibrium S(T(g), T(s); ?) measurements for which T(g) ≠ T(s) provide additional opportunities to characterize the transition state and gas-surface energy transfer at reactive energies. A resistively heated effusive molecular beam doser controls the T(g) of the impinging gas striking the surface. The flux of molecules striking the surface from the effusive beam is determined from knowledge of the dosing geometry, chamber pressure, and pumping speed. Separate experiments with a calibrated leak serve to fix the chamber pumping speed. Postdosing Auger electron spectroscopy is used to measure the carbon of the alkyl radical reaction product that is deposited on the surface as a result of alkane dissociative sticking. As implemented in a typical ultrahigh vacuum chamber for surface analysis, the technique has provided access to a dynamic range of roughly 6 orders of magnitude in the initial dissociative sticking coefficient for small alkanes on Pt(111). 相似文献